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Citations for Promega :
1701 - 1750 of 4304 citations for Human Immunodeficiency Virus Tat Protein HIV 1 Clade C TV019 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... proteins were produced from pcDNA3-based plasmids at 30 °C for 90 min using the in vitro transcription & translation (IVTT) kit (Promega), following manufacturer’s instructions.
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bioRxiv - Neuroscience 2020Quote: ... corresponding samples (PSD-95 and MPP2) were incubated overnight at 37°C with 50 ng trypsin (sequencing grade modified, Promega) in 25 µl of 50 mM ammonium bicarbonate in the presence of heavy water (Campro Scientific GmbH ...
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bioRxiv - Cancer Biology 2019Quote: ... NSCs were then incubated for 45 minutes at 37°C in DNase I solution consisting of 20µl DNase I (Promega RQ1) in 250µl DNase buffer solution ...
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bioRxiv - Molecular Biology 2021Quote: ... FASP was carried out in two 10 kDa MWCO filters with a 50 mM iodoacetamide alkylation step and proteins were digested in 2M urea with 2% wt/wt Lys-C (Wako) for 6 h and 2% modified trypsin (Promega) for 12 h at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 µg of this RNA was treated for 10 minutes at 65 °C with a unit of RQ1 RNAse-free DNase (Promega) in a total reaction volume of 10 µl ...
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Quantitative proteomics reveals remodeling of protein repertoire across life phases of Daphnia pulexbioRxiv - Systems Biology 2019Quote: ... pH 8.5 and digested in a two-step process starting with an overnight digest at room temperature with Lys-C (Wako) followed by six hours of digestion with trypsin (sequencing grade, Promega) at 37 ° C ...
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bioRxiv - Synthetic Biology 2019Quote: ... Backbone and inserts were ligated either for 3 h at RT or overnight at 4°C using T4 ligase (Promega). All vector sequences (Table S5 ...
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bioRxiv - Biochemistry 2019Quote: ... Reactions (3 μL) were incubated for 60 min at 37 °C and quantified with the Nano-glo assay system (Promega).
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bioRxiv - Genomics 2019Quote: ... Tagmentation was performed on 250,000 HCT116 Parental and DKO cells using 700nM Tn5 Transposase assembled using preannealed Tn5ME-A_mC and Tn5ME-B_mC (Table S3) for 30 minutes at 37°C following the addition of 0.01ng of unmethylated Lambda DNA (Promega #D1521). We recommend performing a titration of Tn5 transposase to nuclei input to assay minimum amounts required as in Figure S5 ...
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bioRxiv - Biophysics 2021Quote: ... Samples were incubated at 25°C for 30 minutes with gentle shaking before addition of 50 µL Kinase GloTM Max reagent (Promega) and further incubation at 25°C for 10 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... The sequences ligated by the T4 ligase at 4 °C for 12 h were cloned in the pGEM T-easy vector (Promega). The competent Escherichia coli X10-Gold (Stratagene ...
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bioRxiv - Biochemistry 2020Quote: ... cDNAs were obtained from Origene in pCMV6 vector that also incorporates C-terminal Myc and FLAG tags (Myc-FLAG) or subcloned into a pHTC (Promega)-derived vector that expresses C-terminal HaloTag (41) ...
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bioRxiv - Plant Biology 2021Quote: ... 2 μg of each purified plasmid (pTnT-SEP3-3FLAG and pTnT- AGIAP1-5MyC) were used as input in a 50 μl TnT reaction incubated at 25 °C for 2 hr (Promega). The reaction solution was then combined with 50 μl IP buffer (PBS supplemented with 0.005 % NP40 and proteinase inhibitors (Roche) ...
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bioRxiv - Biochemistry 2021Quote: ... 55 mM IAA at room temperature for 30 min in the dark and digested at 37°C for 16 h with 2 μg porcine trypsin (sequencing grade, Promega). The resultant tryptic peptides were extracted in 40% ACN/0.1% TFA into fresh Protein LoBind® microtubes ...
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bioRxiv - Cell Biology 2021Quote: ... alkylated with 5 mM iodoacetamide for 20 min at room temperature and digested overnight at 37 °C using 13 ng/μl trypsin (Promega). Digested peptides were loaded onto C18-Stage-tips (Rappsilber et al. ...
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bioRxiv - Microbiology 2020Quote: ... and digested for 2 hours at 37°C with ApaI and SpeI restriction enzymes and ligated with T4 DNA ligase (Promega) overnight at 16° to a double-digested pKNG101 plasmid ...
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bioRxiv - Microbiology 2021Quote: ... After 30 min at 60°C the samples were cooled to room temperature and 4 μg of Sequencing Grade Trypsin (Promega) for digestion were added ...
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bioRxiv - Microbiology 2021Quote: ... samples were washed-dried once again and digested overnight at 37°C using mass spectrometry grade Trypsin gold (Trypsin Gold, Promega) at a concentration of 12.5 ng/μl in 50 mM ammonium bicarbonate and 10 mM CaCl2 ...
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bioRxiv - Microbiology 2020Quote: ... The cells were then left at 32°C or moved to 37°C for 9 hours before read on a luminometer using the Dual-Luciferase Reporter Assay Step (Promega) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Plates were frozen for at least 30 minutes at −80°C before thawing and reading on a GloMax Multi plate reader (Promega). Briefly ...
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A Bidirectional Switch in the Shank3 Phosphorylation State Biases Synapses toward Up or Down ScalingbioRxiv - Neuroscience 2021Quote: ... Proteins were digested with endoproteinase LysC (Wako Laboratories) for 2 h at 25 °C followed by overnight digest with sequencing-grade trypsin (Promega) at 25 °C (enzyme-to-substrate ratios of 1:50) ...
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bioRxiv - Cell Biology 2021Quote: ... which were washed with a series of harsh washes before cross-linker reversion and on bead digest with Trypsin Lys-C (Promega). Peptides were then desalted and prepared for mass spectrometry ...
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bioRxiv - Cell Biology 2021Quote: ... On-bead digestion was performed by overnight incubation at 37°C with 2 μg of mass spectrometry grade trypsin (Promega). The following morning an additional 2 μg of trypsin was added to each sample and incubated at 37°C for 4-6 hours ...
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bioRxiv - Cell Biology 2021Quote: ... while the sequences for NSD2 or NSD3 PWWP1 domain was cloned in-frame into a pNLF1-C [CMV/Hygro] vector from Promega using the In-Fusion HD Cloning kit (Takara) ...
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bioRxiv - Cell Biology 2021Quote: ... NY) and washed with S-Trap buffer prior to overnight proteolysis at 37°C with LysC/trypsin (Promega, Madison, WI) in 50 mM ammonium bicarbonate (pH 8.0 ...
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bioRxiv - Neuroscience 2020Quote: ... 8 min, 4 °C) and resuspended in staining buffer (PBS, pH 7.4, with 0.5% RNase-free BSA (Promega, Heidelberg, Germany) and 0.2 U/μl of RNasin Plus RNase inhibitor (Promega)) ...
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bioRxiv - Cell Biology 2022Quote: ... The cell plates were incubated at 37°C for 48-60 hours before 50 μL of CellTiter-Glo reagent (Promega) was added ...
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bioRxiv - Cell Biology 2022Quote: ... Trapped proteins were washed four times with the methanol TEAB buffer and then digested overnight at 37°C using 1ug Trypsin (Promega). Digested peptides were eluted and dried in a vacuum centrifuge before LC-MS analysis.
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bioRxiv - Biochemistry 2022Quote: ... SpCas9 gRNAs were transcribed at 37 °C for 16 hours using the T7 RiboMAX Express Large Scale RNA Production Kit (Promega). For gRNAs utilized in in vitro cleavage reactions containing SpRY from human cell lysates ...
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bioRxiv - Immunology 2022Quote: ... pH 8.0 using Vivaspin columns with a 3 kDa molecular weight cutoff and digested separately overnight using trypsin chymotrypsin or Glu-C (Mass Spectrometry Grade, Promega) at a ratio of 1:30 (w/w) ...
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bioRxiv - Biochemistry 2022Quote: ... The samples were then diluted to 6 M urea with 50 mM Tris-HCl pH 8 and Trypsin/Lys-C mix (Promega) was added to an enzyme:substrate ratio of 1:25 (w/w ...
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bioRxiv - Cell Biology 2019Quote: ... RT reaction was carried out in 15 ul reaction mixture at 40 °C for 60 min after addition of ImProm II (Promega). DNA fragments for L chain and the variable region of H chain (VH ...
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bioRxiv - Microbiology 2021Quote: ... The virus-serum mix was subsequently used to infect A549-hACE2-TMPRSS2 cells7 for 18 hours at 37 °C before adding ONE-Glo reagent (Promega) for measurement of the luciferase signal by relative luminescence units (RLUs) ...
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bioRxiv - Microbiology 2021Quote: ... primer TTC CGC AAG TTC ACC TAC C and the reverse (3’ – 5’) primer CGG GCC GGC CAT GCT TTA CG with GoTaq Flexi DNA polymerase (Promega) and the following cycling conditions ...
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bioRxiv - Microbiology 2022Quote: All isolates were heat-extracted at 95°C for 20 minutes for polymerase chain reaction (PCR) using GoTaq (Promega M7132) and 8F and 1492R primers to amplify the whole 16S rRNA gene (4) ...
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bioRxiv - Biochemistry 2022Quote: ... The samples were then diluted to 4 M urea with 50 mM Tris-HCl pH 8 and Trypsin/Lys-C mix (Promega) was added to an enzyme:substrate ratio of 1:250 (w/w ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein precipitation was done using 6 volume of ice cold acetone and digested overnight at 37 o C using modified trypsin (Promega). Then peptides were fractionated by bRPLC and 8 fractions were collected ...
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bioRxiv - Microbiology 2020Quote: ... One aliquot was stored at −80°C for later infectivity analyses and the other aliquot was treated with DNAse I (RQ1 DNAse, Promega) prior to purification of viral DNA using a PureLink Viral RNA/DNA minikit (ThermoFisher) ...
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bioRxiv - Microbiology 2021Quote: ... The virus-serum mix was subsequently used to infect A549-hACE2-TMPRSS2 cells [41] for 18 hours at 37°C before adding ONE-Glo reagent (Promega) for measurement of the luciferase signal by relative luminescence units (RLUs) ...
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bioRxiv - Microbiology 2020Quote: ... coli was grown in NB liquid medium (180 rpm, at 30 ° C) and had its DNA extracted with Wizard Genomic DNA Purification Kit (Promega). The number of bacteria in the seed and root was calculated based on the values of Ct (cycle threshold ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were incubated for 3 days at 35°C with 5% CO2 and cell viability was evaluated using the CellTiter Glo (Promega) post the 3-day incubation ...
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bioRxiv - Cancer Biology 2021Quote: The full length 3’ UTR of c-Myc was cloned into the pmirGLO Dual-Luciferase miRNA target expression vector (Promega). Luciferase activity was assayed in transfected cells using the Dual-Luciferase Reporter Assay (Promega) ...
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bioRxiv - Systems Biology 2021Quote: ... alkylated in the dark with 2 mM iodoacetamide for 45 minutes at room temperature and digested overnight at 37°C with 80 ng trypsin (Promega). Following digestion ...
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bioRxiv - Molecular Biology 2021Quote: The 1-450bp DNA region of the luciferase coding sequence was synthesized with G/C content optimized and ‘ATG’ codons removed and then inserted into the pGL3-Promoter vector (E1761, Promega) in place of the original full-length luciferase coding region using ClonExpress®II One Step Cloning Kit (C112-01 ...
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bioRxiv - Microbiology 2020Quote: ... protein lanes were cut into 10 pieces, destained (600 rpm, 37 °C, 200 mM NH4HCO3 in 30% acetonitrile) and digested with trypsin (sequencing grade, Promega) overnight at 37 °C ...
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bioRxiv - Microbiology 2020Quote: ... coli was grown in NB liquid medium (180 rpm, at 30 °C) and had its DNA extracted with Wizard Genomic DNA Purification Kit (Promega). The reference DNA was diluted in series of 10−2 to 10−8 (20 – 2 × 109 ng of DNA) ...
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bioRxiv - Microbiology 2020Quote: ... Reduced and alkylated proteins from band slices excised from the entire separation profile were in-gel digested at 37°C overnight with 100 ng trypsin (modified sequencing grade, Promega). Supernatants were dried in a vacuum centrifuge and resuspended in 0.1% TFA ...
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bioRxiv - Immunology 2019Quote: ... Samples were diluted 10-fold with 50 mM Triethylammonium bicarbonate buffer at pH 8 and incubated overnight at 37°C with sequencing grade trypsin (Promega) added at a 1:50 enzyme:substrate ratio (wt/wt).
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bioRxiv - Biochemistry 2019Quote: ... for the indicated times at 37 °C and activity was measured using the Dual-Glo Luciferase Assay System (Promega, E2940) in a Tecan Infinite M200 Pro multi-well plate reader ...
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bioRxiv - Cell Biology 2019Quote: ... Samples were diluted 10-fold with 50 mM triethylammonium bicarbonate buffer at pH 8 and incubated overnight at 37°C with sequencing grade trypsin (Promega) at a 1:50 enzyme:substrate ratio (wt/wt).