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Citations for Promega :
1751 - 1800 of 4304 citations for Human Immunodeficiency Virus Tat Protein HIV 1 Clade C TV019 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: Transcription reactions were carried out for 20 minutes at 37 °C in a reaction volume of 250 μL containing 20 μg of plasmid template in 1x transcription buffer (Promega; 40 mM Tris-HCl pH 7.9 / 6 mM MgCl2 / 10 mM spermidine / 50 mM NaCl ...
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bioRxiv - Zoology 2021Quote: ... The cells transfected with different recombinant vectors were cultured for additional 36 h at 37 °C for transcriptional activity analysis by using the Dual-Glo Luciferase Assay System (Promega) with a luminometer (Promega ...
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bioRxiv - Microbiology 2020Quote: ... enriched proteins were digested at 37 °C overnight on agarose beads in RapiGest SF solution (Waters, 186001861) supplemented with 0.75 μg trypsin (Promega, V511A). The reaction was stopped using 1% TFA (Sigma ...
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bioRxiv - Biochemistry 2020Quote: ... and bound proteins were digested for ∼14 hr at 37°C in 200 μL of trypsin premix solution containing sequence grade trypsin (2 mg, Promega), urea (0.5 M in 200 mM EPPS pH 8.4) ...
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bioRxiv - Microbiology 2019Quote: ... After 1 h incubation at 37°C the sample was cooled down and mixed with 30 Weiss Units of T4 DNA Ligase (Promega) and incubated for 4 h in a water bath at 16°C ...
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bioRxiv - Cancer Biology 2021Quote: ... and pellets were resuspended in 100 μl of 100 mM triethylammonium bicarbonate (TEAB) and subsequently digested overnight at 37 °C by adding 1.1 μg of sequencing-grade modified porcine trypsin (Promega, V5113). TMT reagents were reconstituted in 40 μl of anhydrous acetonitrile ...
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bioRxiv - Immunology 2021Quote: ... extensively washed to remove unbound virus and co-cultured with Huh7.5 for 5 days at 37°C after which they were analyzed for with the Luciferase assay system (Promega, USA) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... proteins were reduced by dithiothreitol and alkylated by iodoacetamide in the dark at room temperature, followed by in-solution digestion sequentially using lysyl endopeptidase (Lys-C, Wako) for 3 h and trypsin (Promega) overnight at room temperature as previously described (Paul et al. ...
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bioRxiv - Cancer Biology 2020Quote: ... digestion was continued by incubation of the sample at 37°C for 6 hours with trypsin (Promega, Catalog Number V5113) at an enzyme to substrate ratio of 1:75 ...
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bioRxiv - Biophysics 2022Quote: ... In vitro transcription reactions were incubated for 16 hours at 37 °C and then treated with 45 U/mL of RQ1 DNase (Promega) at 37 °C for 30 minutes ...
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bioRxiv - Evolutionary Biology 2022Quote: ... or RepNDK-RNA was incubated at 37 °C for 12 h in a translation system and FluoroTect GreenLys tRNA (Promega), with neither CTP nor CDP to preclude RNA replication ...
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bioRxiv - Plant Biology 2022Quote: ... The gel pieces of the different samples were washed and digested over night at 37 °C with MS-grade trypsin (Promega). MS/MS analysis was done using a previously described setup of a Waters nanoACQUITY gradient UPLC (www.waters.com ...
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bioRxiv - Physiology 2022Quote: ... Denatured proteins were then enzymatically digested into peptides upon incubation first with endoproteinase LysC (Wako Laboratories) at 25 °C shaking for 2 h and then with sequencing-grade trypsin (Promega) at 25 °C shaking overnight ...
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bioRxiv - Microbiology 2022Quote: ... at 72° C for 10 min to add 3’-A overhangs for T/A cloning with pGEM-T Easy plasmid (Promega). A 610 bp DNA fragment upstream of flgV was amplified using PCR primers 85 and 86 ...
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bioRxiv - Microbiology 2022Quote: Salmonella strains were cultured overnight at 37°C in LB broth and DNA was extracted using a Maxwell RSC 48 instrument (Promega) and associated Maxwell RSC cultured cells DNA kit (Promega) ...
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bioRxiv - Molecular Biology 2023Quote: ... Oligos were then ligated at 4°C overnight to the appropriate digested ACR-16-REM sequence (T4 ligase, Promega, USA) and transformed into DH5α (ThermoScientific ...
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bioRxiv - Microbiology 2023Quote: ... The plate was incubated for 48 hours at 37°C in a CO2 incubator and luciferase activity was quantified by adding nano-glo luciferase substrate (Promega). The luminescence was recorded by using Cytation 5 plate reader (BioTek inc) ...
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bioRxiv - Molecular Biology 2022Quote: ... the Immunoprecipitated samples were in-solution digested overnight at 37 °C in 400 ng of mass spectrometry grade trypsin (Promega) enzyme ...
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bioRxiv - Molecular Biology 2022Quote: ... expressing PALLD and pNLF1-N [CMV Hygro] or pNLF1-C [CMV Hygro] vector (Promaga) expressing ANKRD1 were treated with 100 nM HaloTag NanoBRET 618 Ligand (Promega), whereafter signals were detected six hours later using a Synergy 4 instrument (BioTek) ...
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bioRxiv - Plant Biology 2022Quote: ... Hybridization probes were generated by PCR amplifi cation using gene specifi c primers (Table S1) and radiolabeled with the Prime A Gene labeling kit (Promega) following the recommendations of the manufacturer.
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bioRxiv - Systems Biology 2022Quote: ... and digested with 3 μg LysC (Wako) O/N at 37°C and then with 3 μg of trypsin (Promega) for eight hours at 37°C following FASP procedure (Filter-aided sample preparation 48) ...
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bioRxiv - Microbiology 2022Quote: ... The plates were incubated at 37°C for ~12 hours before luciferase activity was quantified using the Luciferase Assay System (Promega) using SoftMax Pro v.7.0.2 (Molecular Devices) ...
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bioRxiv - Microbiology 2022Quote: ... and alkylated with 5 mM of iodoacetamide 30 min in the dark at RT°C prior to digestion with 5 μg of trypsin Gold (Promega) for 15 h at 37 °C ...
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bioRxiv - Molecular Biology 2023Quote: Protein samples were treated with Endoprotease Lys-C (Fujifilm Wako chemicals, Osaka, Japan) and Trypsin (Trypsin Gold Mass Spec Grade, Promega). Peptide samples were desalted using OMIX C18 pipette tips (Agilent Technologies) ...
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bioRxiv - Molecular Biology 2023Quote: ... The mixture was incubated at 25°C for 30 minutes and the production of ADP was measured using the ADP-Glo kinase assay kit (Promega). Vmax was calculated after fitting the kinase activity data to the Michaelis–Menten equation ...
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bioRxiv - Biochemistry 2023Quote: ... Translation reactions were incubated for 23 min at 30 °C and nanoluciferase activity was monitored using the Nano-Glo Luciferase Assay Kit (Promega) in a Microplate Luminometer (Veritas) ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were subjected to in gel tryptic digest overnight at 37 °C with approximately 2 µg trypsin per 100 µl gel volume (Trypsin Gold, mass spectrometry grade, Promega). Peptides were eluted twice with 100 mM NH4HCO3 followed by an additional extraction with 50 mM NH4HCO3 in 50% acetonitrile ...
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bioRxiv - Developmental Biology 2023Quote: Protein extracted from 10 CNC explants as described above or immunoprecipitated with the Flag antibody from embryos or Hek293T cells were diluted in 8M urea and processed with Trypsin/Lysine-C according to manufacturer instruction (Promega). Tandem mass spectrometry was performed using a Thermo orbitrap Fusion (Mass spectral data were obtained at the University of Massachusetts Mass Spectrometry Core Facility ...
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bioRxiv - Biochemistry 2023Quote: ... stable HEK293T cells expressing wild type COMT were transiently transfected with pTagBFP-C expressing the KDEL marker using FugeneHD (Promega) following the manufacturer’s instructions with 100 ng plasmid ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with or without 50 μM adenosine 5′-triphosphate (ATP) at 30 °C for 30 minutes followed by kinase assays using ADP-Glo Kinase Assay kit (Promega). Data were analyzed using GraphPad Prism 9 ...
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bioRxiv - Cell Biology 2022Quote: ... Tris 50mM pH8.0 and samples were heated 5 min at 90°C before digestion in a mix of 500 ng of LysC (Promega) at 37°C for 2h ...
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bioRxiv - Developmental Biology 2023Quote: ... including the identified m6A site (wildtype sequence or A-to-C mutation) was cloned into the pmirGLO Dual-Luciferase miRNA Target Expression Vector (Promega), per manufacturer ’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... samples resuspended in 5%SDS buffer were reduced with DTT and alkylated, followed by digestion on a S-Trap column (ProtiFi, LLC)with sequencing-grade Lys-C/trypsin (Promega). The resulting peptides were were analyzed with a nanoAcquity UPLC system (Waters ...
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bioRxiv - Developmental Biology 2023Quote: ... Sample cleanup was performed using the SP3 method 83 and on-bead digestion was performed overnight using Trypsin/Lys-C (Promega). Eluted tryptic peptides from SP3 beads were pre-fractionated by high pH reverse phase chromatography into 96 individual fractions using a 64-minute gradient from 1% to 50% solvent B (A ...
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bioRxiv - Biochemistry 2023Quote: ... Cell culture medium was replaced with pre-heated (37 °C) fresh medium containing EnduRen (60 µM) or 1x Nano-Glo Endurazine (Promega). Luciferase activity was measured using LuminoCell device ...
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bioRxiv - Plant Biology 2023Quote: ... Leaf samples of the high dyad producing plants were stored at -80°C before DNA extraction with the Wizard genomic DNA purification kit (Promega). A pooled DNA sample (equal mass ...
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bioRxiv - Microbiology 2023Quote: ... In vitro mRNA synthesis was then carried out at 37 °C for 4 h in T7 RiboMAX Large Scale RNA Production System kit (Promega) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2023Quote: ... In solution digests were performed overnight at pH 8 and 37 °C with mass spectrometry grade trypsin (Promega, Madison, WI) at a 1:33 enzyme-to-protein ratio and quenched with formic acid (0.15%) ...
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bioRxiv - Biochemistry 2023Quote: ... for 30 min at 37□C (For NVX-CoV2373, 9 different conditions were used viz., 16 h of trypsin (Promega) digestion ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were incubated for 24hr at 37°C and cell death was measured through a lactate dehydrogenase (LDH) assay using the CytoTox 96 kit (Promega). 50 μL of media was collected from all experimental wells with two replicates each and placed in a fresh 96-well plate ...
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bioRxiv - Developmental Biology 2023Quote: ... Wnt3A was added and incubated for another 6h at 37°C and luciferase activity was measured using the Dual-Luciferase Assay System (Promega). Each measurement was repeated with at least three independent transfections.
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bioRxiv - Cancer Biology 2023Quote: ... In-solution digestion was performed overnight on a shaker set at 37 °C using sequencing grade trypsin (Promega, Madison, WI) at ∼1:25 w/w enzyme/protein ratio ...
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bioRxiv - Molecular Biology 2024Quote: ... 40 µL of digestion solution (1.25 mM TCEP, 5 mM chloroacetamide, 0.2 µg trypsin/Lys-C mix (Promega, cat#V5073), in 100 mM HEPES pH 8.5 ...
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bioRxiv - Microbiology 2024Quote: ... cells were infected at MOI 1 for 24 h and cells were frozen dry at -80°C for 30 min and lysed in Passive Lysis Buffer (Promega). Bioluminescence was measured using the Nano-Glo® Luciferase Assay System (Promega ...
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bioRxiv - Cell Biology 2024Quote: ... Digestion was performed over-night at 37°C by addition of 20 µL of trypsin (Sequencing Grade Modified Trypsin, Promega) at 37.5 ng/µL in 50 mM ammonium bicarbonate ...
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bioRxiv - Physiology 2024Quote: ... Cysteine residues were alkylated in presence of 20 mM iodoacetamide at 37 °C in the dark for 30 min and tryptic digestion (sequencing grade, Promega) was performed at a 100:1 protein to enzyme ration at 37 °C overnight ...
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bioRxiv - Microbiology 2023Quote: ... The inoculum was added to HEK293T cells and incubated at 37 ° C with 5% CO2 and the luciferase signal was with analyzed using luciferase assay kit (Promega).
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bioRxiv - Cancer Biology 2024Quote: ... The solution was then diluted to 4 mL with 50 mM ammonium bicarbonate and subjected to overnight digestion at 37°C with trypsin (Promega) using an enzyme to substrate ratio of 1:200 (w/w) ...
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bioRxiv - Cancer Biology 2024Quote: ... Reactions were incubated for 1.5 hr at 30 °C and luciferase signal was measured using the Dual-Glo Luciferase Assay System (Promega E2920).
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bioRxiv - Microbiology 2024Quote: ... In vitro mRNA synthesis was then carried out at 37 °C for 4 h in T7 RiboMAX Large Scale RNA Production System kit (Promega) according to the manufacturer’s recommendations ...