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Citations for Promega :
1501 - 1550 of 4304 citations for Human Immunodeficiency Virus Tat Protein HIV 1 Clade C TV019 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2019Quote: ... Proteins were in-gel digested using 0.6 µg of modified sequencing grade trypsin (Promega) in 50 mM ammonium bicarbonate overnight at 37°C ...
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bioRxiv - Developmental Biology 2019Quote: ... we produced recombinant H3 mutant proteins from mRNAs using rabbit reticulocyte lysate (Promega L4600). After 3h of incubation at 4°C in interphase extracts followed by another 3h incubation with anti-HA beads ...
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bioRxiv - Developmental Biology 2020Quote: ... [35S]-labeled Zc3h10 protein was produced by using TNT coupled transcription/translation kit (Promega). 20 ug of GST fusion proteins were incubated for 2 hrs at 4°C with in vitro translated Zc3h10 and glutathione sepharose beads ...
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bioRxiv - Plant Biology 2020Quote: ... and used for protein digestion with sequencing-grade modified trypsin (Promega, Madison, WI, USA). Resulting peptides were purified using Sep-Pak Vac tC18 100 mg cartridge (Waters ...
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bioRxiv - Synthetic Biology 2020Quote: ... Proteins were digested with two separate and sequential aliquots of sequencing grade trypsin (Promega) of 1 μg ...
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bioRxiv - Developmental Biology 2020Quote: ... and 200 ng purified recombinant mouse DLX2 protein in Gel Shift Binding Buffer (Promega). Unlabeled probe was added for ‘cold’ competition ...
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bioRxiv - Physiology 2020Quote: The protein solution samples were hydrolyzed into peptide segments by trypsin (V9012, Promega (Beijing) Biotech Co. ...
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bioRxiv - Biochemistry 2021Quote: ... the cells were transiently transfected with tagged-target proteins using Fugene® HD (Promega) following manufacturer’s recommendations ...
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bioRxiv - Microbiology 2021Quote: ... proteins were digested ingel using modified trypsin (sequencing grade, Promega, Charbonnières les Bains/France) as previously described (50).
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bioRxiv - Biochemistry 2022Quote: ... ⑤Resuspended the denatured proteins with 25 mmol/L NH4HCO3 and digested with trypsin (Promega, Fitchburg ...
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bioRxiv - Plant Biology 2022Quote: ... PsiYAB11 protein was expressed using the TNT SP6 Coupled Wheat Germ Extract System (Promega). The protein-bound beads were incubated with 50 ng of adaptor-ligated gDNA fragments on a rotator for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: In-gel protein digestion of every spot was performed using sequencing-grade trypsin (Promega) as described elsewhere (26) ...
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bioRxiv - Molecular Biology 2022Quote: Fifty μl protein expression reaction using TNT SP6 Coupled Reticulocyte Lysate System (Promega, L4600) containing 1000 ng pIX-Halo-bZIP plasmids was incubated for 3 h at 30°C ...
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bioRxiv - Molecular Biology 2022Quote: ... proteins were precipitated with acetone and trypsin-digested using sequencing grade-modified trypsin (Promega). The resulting peptide mixture was purified by STAGE-TIP method using a C18 resin disk (3M Empore ...
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bioRxiv - Plant Biology 2022Quote: ... Proteins eluted at pH 9.0 were further treated with 5 U of CIAP (Promega) and the buffer provided by the manufacturer (a negative control was prepared without phosphatase) ...
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bioRxiv - Synthetic Biology 2022Quote: ... The proteins were digested with sequencing-grade modified trypsin (Promega Corporation, Madison, WI, USA). The peptides were analyzed using a nano LC–MS system (UltiMateTM 3000 RSLCnano and Orbitrap ExplorisTM 240 ...
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bioRxiv - Molecular Biology 2022Quote: ... proteins were digested with chymotrypsin (5 ng/μL) at 25ºC overnight (Trypsin gold, Promega). Digestion was stopped by addition of 5% formic acid and peptides extracted twice with 70% acetonitrile and 5% formic acid (10 min sonication) ...
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bioRxiv - Systems Biology 2023Quote: ... dark) and digestion using 0.015 µg Trypsin for 1.5 µg protein (Trypsin Gold, Promega, Fitchburg ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were digested by trypsin (Trypsin Gold, Mass Spectrometry Grade, Promega; protein:enzyme (w/w) ratio – 100:1 ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were then digested into peptides with sequencing grade modified trypsin (Promega V5113) at 37 °C overnight ...
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bioRxiv - Biophysics 2023Quote: ... the desired volume of protein solution was incubated with Factor Xa (Promega, Madison, WI). All experiments were carried out in a tris buffer (150 mM NaCl ...
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bioRxiv - Cell Biology 2023Quote: ... and proteins were further digested by the addition of 2 μg trypsin (Promega #V5113) for 4 hours at room temperature on a shaker ...
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bioRxiv - Physiology 2023Quote: ... Chemiluminescent detection of in vitro translated protein was performed following the manufacturer’s protocol (Promega).
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bioRxiv - Biochemistry 2024Quote: ... The HaloTag-JF503-SWSAP1 was quantified by using recombinant HALO-GFP protein standard (Promega) pre-incubated with JF503 at a 1:1 molar ratio and titrated into single molecule buffer (20 mM Tris pH7.5 ...
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bioRxiv - Microbiology 2024Quote: ... Prior to tryptic digestion of the protein overnight using mass-spec grade Trypsin (Promega), protein was reduced and alkylated using dithiothreitol and iodoacetamide ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were digested using filter-aided sample preparation with sequence-grade trypsin (Promega, # V5111) at 37°C overnight ...
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bioRxiv - Bioengineering 2024Quote: ... The reactions were performed using the S30 T7 High-Yield Protein Expression System (Promega). 3 μl of peptide solution were added to reaction mixtures to get a final concentration respectively of 32 μg/ml or 512 μg/ml in a final volume of 15 μL in nuclease-free PCR tubes ...
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bioRxiv - Microbiology 2024Quote: ... The proteins were resuspended in 100 mM NH4HCO3 and digested with trypsin (Promega, UK) at an enzyme-to-substrate ratio of 1:20 and protease max 0.1% (Promega ...
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bioRxiv - Biochemistry 2024Quote: ... the blotted proteins were developed using Nano-Glo® In-Gel Detection System (Promega) and scanned with a ChemiDoc MP Imaging System (Bio-Rad).
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bioRxiv - Molecular Biology 2024Quote: ... 50 ng/μl of mRNA and 6 ng/μl QuantiLum Recombinant Luciferase protein (Promega) were coinjected ...
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bioRxiv - Cell Biology 2019Quote: Proteins were incubated overnight at 37°C with 20 µl of 25 mM NH4HCO3 containing sequencing-grade trypsin (12.5µg/ml, Promega) at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... The sample was digested for 2 h with 0.5 µg of endoproteinase lysine-C (Wako chemicals) and then digested with 0.5 µg of trypsin (Promega) overnight ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were incubated at 37°C for 72 hours and cell viability was measured using Cell TiterGlo (Promega). Percent viability of treated wells was calculated by normalizing to the average Cell TiterGlo values from untreated wells.
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bioRxiv - Cell Biology 2019Quote: ... RNAs were subsequently generated by in vitro transcription overnight at 37°C using RiboMAX transcription kits (Promega Corporation). After RQ1 DNAse digestion of the template cDNA ...
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bioRxiv - Genomics 2020Quote: ... Beads were resuspended in 50μL 100mM AMBIC and incubated overnight at 37°C with 400ng Trypsin (Promega V5280). Beads were supplemented with an additional 400ng Trypsin and incubated for a further 4 hours at 37°C ...
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bioRxiv - Cell Biology 2019Quote: ... Samples were diluted two-fold again and incubated overnight at 37 °C with trypsin (sequencing grade modified, Promega) at a 1:50 (w/w ...
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bioRxiv - Biochemistry 2020Quote: ... Enzymatic digestion of was performed overnight at 37°C by the addition of sequencing grade trypsin (Promega, Wisconsin) (100 ng per sample ...
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bioRxiv - Cancer Biology 2021Quote: ... the protein extracts were then incubated overnight at 37°C with 20 μl of 25mM NH4HCO3 containing sequencing-grade trypsin (12.5μg/ml, Promega). The resulting peptides were desalted using ZipTip µ-C18 Pipette Tips (Millipore ...
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bioRxiv - Molecular Biology 2022Quote: ... at 72°C for 20min and then TA cloned into the pGEM-T Easy vector (Promega, Madison, WI). The cloned PCR products were Sanger Sequenced with M13 Forward (5’-TGTAAAACGACGGCCAGT-3’ ...
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bioRxiv - Microbiology 2020Quote: ... emission 410 nm) was immediately measured every 15 minutes at 37°C using a GloMax plate reader (Promega).
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bioRxiv - Neuroscience 2020Quote: ... The mixture was then and incubated for 7min at 55°C followed by an additional 7 minute incubation with 3.5µl of SDS 0.2% (Promega, #V6551) to ensure that Tn5 was dissociated from the cDNA ...
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bioRxiv - Molecular Biology 2019Quote: 1875 ng of circular plasmids were incubated at 37°C with 50 U of T3 RNA Polymerase (Promega) in a transcription buffer (40 mM Tris-HCl pH 7.9 ...
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bioRxiv - Microbiology 2019Quote: ... were reverse transfected with 50 ng pCAGEN-HA plasmid and 12.5 ng pcDNA3.1+/C-(K)DYK protease-plasmid using Fugene 6 (Promega). After 24 h ...
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bioRxiv - Cell Biology 2021Quote: ... the samples were diluted to 1.5 M urea using 20 mM HEPES (pH 8.5) and digested at 30°C overnight with trypsin (Promega) at a ratio of 70:1 (w/w) ...
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bioRxiv - Developmental Biology 2021Quote: ... into 100 mM ammonium bicarbonate and digested overnight at 37 °C with mass spectrometry grade trypsin gold (Promega) at a 1:50 trypsin:protein ratio ...
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bioRxiv - Microbiology 2020Quote: ... where the samples were destained and subjected to in-gel proteolytic digestion with trypsin/Lys-C mixture (Promega). The digests were extracted ...
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bioRxiv - Microbiology 2022Quote: ... The concentrated phages were next treated for 30 min at 37°C with 10 μL of DNAse (Promega) and 2.5μL of RNAse (Macherey-Nagel ...
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bioRxiv - Biophysics 2022Quote: ... and the two halves were digested with either Sequencing Grade Modified Trypsin or endoproteinase Glu-C (both Promega, Madison ...
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bioRxiv - Molecular Biology 2023Quote: ... Enzymatic digestion was performed at 37°C and overnight with 500 ng trypsin (Promega, Charbonnieres les Bains, France). Peptide mixtures were then desalted on C18 spin-column and dried in a Speed-Vacuum.
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bioRxiv - Biochemistry 2023Quote: ... The samples were further processed by digesting them overnight with a Trypsin/Lys-C mix (Promega, Madison, USA) at a 50:1 substrate:enzyme ratio (w/w ...