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Citations for Promega :
1651 - 1700 of 4304 citations for Human Immunodeficiency Virus Tat Protein HIV 1 Clade C TV019 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... with the required amount of cells (cell number equal to the number of wells multiplied by 1.5 × 104).The effector and target cells were mixed 20 h post-transfection at 37°C in fresh medium containing 60μMmembrane-permeable substrate for Renilla luciferase (RL) (Enduren Live Cell Substrate; Promega)1 h prior to mixing ...
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bioRxiv - Cancer Biology 2020Quote: ... Plates were incubated for 72hr at 37°C with 5% CO2 and then measured by CellTiter-Glo assay (Promega). Briefly ...
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bioRxiv - Cancer Biology 2022Quote: ... before the addition of digestion buffer (50 mm Tris) containing 0.8 μg Lys-C (Wako, 129–02541) and 0.8 μg Trypsin-gold (Promega, V5280). Enzymatic digestions proceeded under agitation (400 rpm ...
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bioRxiv - Plant Biology 2022Quote: ... Samples were diluted with 900 µl of 25 mM ammonium bicarbonate and digested overnight at 37°C in the presence of 0.35 µg of sequencing grade modified porcine trypsin (Promega). Peptides were vacuum-dried in a centrifugal evaporator to approximately 250 µl ...
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bioRxiv - Microbiology 2022Quote: ... HEV) or at 33°C (HRV) until harvested by washing in PBS and lysis in Passive Lysis Buffer (Promega). Luciferase activity was determined using either the Renilla Luciferase Assay Kit (Biotium) ...
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bioRxiv - Cell Biology 2022Quote: ... for 30 min at 30° C in the dark and finally tryptically digested with 1.25 µg of sequencing grade porcine trypsin (Promega) in 100 mM ammonium bicarbonate at 37° C for 16 h ...
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bioRxiv - Microbiology 2020Quote: ... alkylated with 100 mM iodoacetamide and proteolytically cleaved over night at 37°C using trypsin (Promega, Madison, WI, USA). Peptides were extracted ...
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bioRxiv - Molecular Biology 2022Quote: ... A second digestion was performed o/n at 37°C while shaking at 1,400 rpm using 500 ng of sequencing grade modified trypsin (Promega). Trypsin digested peptides were separated from Ni-NTA beads by filtering through a 0.45 µm filter Ultrafree-MC-HV spin column (Merck-Millipore).
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bioRxiv - Evolutionary Biology 2022Quote: ... Protein pellets were resuspended in 40μl of 100mM TEAB and digested overnight at 37°C with shaking by the addition of 2μl of 1μg/μl trypsin (Promega, V5111) and 1μl of 80mM CAM (2mM final ...
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bioRxiv - Immunology 2022Quote: ... Transduced cells were incubated for 24 hours at 37°C/5% CO2 and luminescence measured by addition of 40μl of ONE-Glo reagent (Promega) with detection using a Tecan Spark plate reader ...
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bioRxiv - Cancer Biology 2022Quote: ... The beads were finally added with 2.5 μl of 200 ng/µl Trypsin/Lys-C Mix (Cat. No. V5073, Promega) at 37°C with shaking overnight ...
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bioRxiv - Cancer Biology 2019Quote: ... cDNA was stored at −20°C until analysed by polymerase chain reaction using GoTaq®G2 DNA polymerase (Promega) according to the manufacturers protocol ...
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bioRxiv - Synthetic Biology 2020Quote: ... at 54°C for 10 min and the resulting cDNA amplified with GoTaq Flexi DNA polymerase (Promega; Madison, WI) for 8 PCR cycles in the same reaction with a final volume of 100 μL ...
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bioRxiv - Cell Biology 2019Quote: Proteins were then digested overnight at 37°C in 20 μL of 25 mM NH4HCO3 containing sequencing-grade trypsin (12.5 μg/mL; Promega). The resulting peptides were sequentially extracted with 70% acetonitrile ...
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bioRxiv - Bioengineering 2019Quote: ... Ligations of the digested plasmid backbones and PCR products occurred overnight at 16°C using T4 DNA ligase (Promega) prior to transformation into chemically competent Novablue E ...
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bioRxiv - Genetics 2021Quote: ... The samples were reconstituted in 50 µL of 20% acetonitrile 80 mM triethyl ammonium bicarbonate (TEAB) and digested with 3.3 µg of trypsin/Lys-C mix (Promega) at 37C overnight ...
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bioRxiv - Cell Biology 2020Quote: ... The sample was first treated by 2.5 μg of LysC (Wako) for 3 h at 37 °C with shaking and then treated with 2.5 μg of Trypsin (Promega) for over-night at 37 °C with shaking ...
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bioRxiv - Biochemistry 2021Quote: ... Reactions were then incubated at 37 °C for 2 min before the addition of 30 units of SmaI (Promega), 46 kBq [a-32P]-dCTP (111 TBq/mmol ...
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bioRxiv - Neuroscience 2021Quote: ... equilibrated at 20°C for 30 minutes and 30 μl CellTiter-Glo Luminescent Cell Viability Assay reagent (Promega, #G7573) was distributed into each well ...
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bioRxiv - Biophysics 2021Quote: ... the C terminus of MC1R was fused with a 15-amino-acid polypeptide linker (GSSGGGGSGGGGSSG) and a LgBiT (Promega).
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bioRxiv - Molecular Biology 2021Quote: ... Digestion was performed for 8 h at 37°C using 70 ng modified porcine trypsin (Promega, Fitchburg, WI, USA). Tryptic peptides were extracted using 70% acetonitrile and dried using a SpeedVac vacuum concentrator.
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bioRxiv - Neuroscience 2021Quote: ... all DNA constructs were prepared from bacterial cultures grown at 37 °C using PureYield™ Plasmid Midiprep System (Promega) according to manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... Samples were digested initially with LysC (WAKO) at 37°C for 2.5 h and subsequently with 10 μg trypsin (modified sequencing grade, Promega) overnight ...
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bioRxiv - Microbiology 2020Quote: ... was then diluted into fresh 50mM ammonium bicarbonate and incubated overnight at 37°C either with 0.056 μg of trypsin (Promega), chymotrypsin (Sigma Aldrich ...
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bioRxiv - Genomics 2021Quote: ... ligation was performed at 15°C for 16 hours using 100 ng of the digested DNAs and 4.5 U of T4 DNA ligase (Promega) in a final volume of 100 μl ...
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bioRxiv - Molecular Biology 2023Quote: ... A second digestion was performed o/n at 37°C while shaking at 1,400 rpm using 500 ng of sequencing grade modified trypsin (Promega). Trypsin digested peptides were separated from Ni-NTA beads by filtering through a 0.45 μm filter Ultrafree-MC-HV spin column (Merck-Millipore).
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bioRxiv - Microbiology 2022Quote: ... Samples were digested for 2 hr at 25°C with constant agitation with a Trypsin/LysC mix (V5071, Promega), and overnight at 25°C with proteomics-grade porcine trypsin (T6567 ...
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bioRxiv - Systems Biology 2023Quote: ... cooled on ice to room temperature for 5 min and digested overnight at 37 °C with 0.5 μg of sequencing-grade trypsin (Promega). Peptide mixtures were acidified to pH 3 with 1.5 µl 5% FA ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then incubated for 30 min at 4 °C slowing rotating with Passive Lysis Buffer (Promega Cat#E1941). After incubation with the lysis buffers ...
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bioRxiv - Molecular Biology 2023Quote: ... Full-length GID4 N or C-terminal NanoLuc-fusion was transfected into HEK293T cells using FuGENE HD (Promega, E2312) according to manufacturer’s instructions and proteins were allowed to express for 20 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... After incubation at 37 °C/5 % CO2 for 20 h 40 nl HaloTag® NanoBRET™ 618 Ligand (PROMEGA) was added to the cells using an Echo acoustic dispenser (Labcyte ...
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bioRxiv - Cell Biology 2023Quote: ... we isolated individual clones and screened for expression using the C-terminal nanoluc fusion (Nanoluc Lytic Detection Kit, Promega), followed by additional characterization of SMO agonist-induced ciliary accumulation via microscopy ...
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bioRxiv - Systems Biology 2023Quote: ... Plates were incubated at 37°C for 96 hours and lysed using CellTiter-Glo Reagent (Promega, catalog. no. G7570). Luminescence was measured using a PHERAstar FS instrument and growth inhibition was calculated relative to DMSO-treated wells.
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bioRxiv - Biochemistry 2023Quote: ... samples were washed twice with S-Trap buffer before overnight digestion at 37° C with Trypsin (Promega Cat# V511X) in digestion buffer (50 mM ammonium carbonate ...
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bioRxiv - Genomics 2023Quote: ... The column was washed twice with 150 µL of loading buffer and gently centrifuged before overnight digestion at 37°C with 0.1 µg/µL of trypsin (Promega) in 50 mM ammonium bicarbonate ...
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bioRxiv - Cell Biology 2023Quote: DNA was prepared from bacterial cultures grown at 37°C using a Midiprep kit (PureYield Plasmid Midiprep System, Promega) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... at 10,000 x g and 4°C for 2 min and digested by addition of RNAse-free DNAse I (Promega) for 10 min on ice ...
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bioRxiv - Biochemistry 2023Quote: ... each sample was digested overnight at 37 °C with one or more of the following enzymes separately: trypsin (Promega), Lys-C (Roche) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Probe synthesis reactions were performed at 37 °C for 3 h and then were treated with DNase I (Promega) at 37 °C for 20 min ...
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bioRxiv - Pathology 2023Quote: ... Overnight digestion was performed at 37 °C with 2 μL of freshly prepared trypsin at 0.1 μg/μl (V5111, Promega) in 50 mM ammonium bicarbonate (per 100 μl of sample) ...
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bioRxiv - Microbiology 2023Quote: ... Plates were then incubated at 37°C overnight before luciferase activity was quantified using the Luciferase Assay System (Promega) on SoftMax Pro v.7.0.2 (Molecular Devices) ...
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bioRxiv - Microbiology 2023Quote: ... OD600nm was monitored every hour at 37°C for 24 h by using a GloMax Explorer plate-reader (Promega).
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bioRxiv - Cancer Biology 2023Quote: ... beads were reconstituted in 5 μL 50 mM HEPES pH 8.0 buffer containing trypsin/rLys-C enzyme mix (Promega) at a 1:25 enzyme to protein ratio ...
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bioRxiv - Developmental Biology 2024Quote: ... Washes were performed 6x with 200 µL each and digestion was performed with 0.75 µg Trypsin/Lys-C Mix (Promega) per 10 µg total protein ...
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bioRxiv - Cell Biology 2019Quote: ... diluted 1/500 in PBS-BSA 1% and 0.4 U.mL-1 RNAsin (Promega). We visualized and quantified 5-FUrd incorporation by using high content imaging device (OPERETTA ...
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bioRxiv - Cancer Biology 2021Quote: ... short tandem repeat analysis verified the identity of mouse (Bioassay Methods Group, National Institute of Standards and Technology) and human (Promega GenePrint 10 System) cells.
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bioRxiv - Microbiology 2019Quote: ... These stock solutions were then diluted to the indicated concentrations in sequencing-grade water and 10 ng commercial human DNA (Promega Corp cat#G3041) was added to all samples ...
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bioRxiv - Synthetic Biology 2022Quote: Cytotoxicity assay was performed on HCT116 human colon cells (ATCC, #CCL-247™) using CellTiter 96® AQueous One Solution Cell Proliferation Assay (Promega, #G3580) which is a colorimetric method based on MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2- (4-sulfophenyl)-2H-tetrazolium ...
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bioRxiv - Systems Biology 2023Quote: ... Peptide loading was assessed by comparing the intensity of the total ion current to 200 ng of a human K562 whole cell lysate standard (Promega, Madison, Wisconsin, USA) prepared at 0.2 μg/μL.
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Development of a genetically-encoded sensor for probing endogenous nociceptin opioid peptide releasebioRxiv - Neuroscience 2023Quote: HEK293 cells growing at 70% confluency in a 10 cm dish were transfected with wild-type human NOPR or NOPLight (3 µg DNA) and GloSensor-20F (Promega, 2.5 µg DNA) using 12 µL Lipofectamine 2000 (Thermo Fisher ...