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Citations for Promega :
1651 - 1700 of 5634 citations for Retinol Binding Protein RBP Multi format ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... After the sequential digestion with LysC and trypsin (Promega, each protease to protein ratio 1:80), beads were retained with a magnetic rack and samples were filtered with Costar Spin-X spin filters (0·22 µm cut-off ...
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bioRxiv - Synthetic Biology 2023Quote: ... Protein was quantified using the Nano-Glo® HiBiT Extracellular Detection System (N2420, Promega, Madison, WI) using the HiBiT Control Protein (20 µM ...
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bioRxiv - Biophysics 2023Quote: ... The purified fused protein was cleaved by treating it with Factor Xa (FXa; Promega, Madison, WI) at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... and proteins were digested overnight with LysC (FUJIFILM Wako) and trypsin (sequence grade; Promega, Madison, WI) at the protein:enzyme ratio of 100:1 for each enzyme at 25 °C ...
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bioRxiv - Biochemistry 2023Quote: ... 10 µg of reconstituted protein was digested in 0.05 N HCl with 1 µg pepsin (Promega) without prior reduction/alkylation at a volume of 200 µl 50 °C for 3 h ...
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bioRxiv - Systems Biology 2023Quote: ... pH 8.0 were added and proteins were digested with 1.5 μl trypsin (0.5 μg/μl; Promega) with constant shaking at 1,100 rpm and incubation at 37 °C on a ThermoMixer C incubator for 4 h ...
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Short-range interactions between fibrocytes and CD8+ T cells in COPD bronchial inflammatory responsebioRxiv - Pathology 2023Quote: Proteins were digested by incubating each gel slice with 10 ng/µl of trypsin (V5111, Promega) in 40 mM NH4HCO3 ...
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bioRxiv - Microbiology 2023Quote: ... The protein extracts were digested by Lys-C endoproteinase and then by trypsin (Trypsin Gold, Promega). Peptides were vacuum-dried and resuspended in 100 µl of solvent A (0.1% (v/v ...
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bioRxiv - Cell Biology 2023Quote: ... 35S-labeled proteins were synthesized in vitro using the TnT T7 coupled reticulocyte lysate system (Promega). Translation reaction products from 0.25 μg of plasmid DNA were incubated with GST-labeled proteins on glutathione-Sepharose beads in NETN-E buffer (50 mM Tris ...
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bioRxiv - Microbiology 2023Quote: ... the pcDNA3.1+ expression plasmids for spike proteins were co-transfected using Fugene (Promega, Madison, WI, USA) into HEK293T/17 cells ...
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bioRxiv - Systems Biology 2024Quote: ... proteins were digested overnight at 37 °C and 800 rpm using sequencing-grade modified trypsin (Promega) at a protein-enzyme-ratio of 50:1 ...
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bioRxiv - Neuroscience 2024Quote: ... Protein samples (15 µg) were digested overnight at 37 °C with trypsin (Promega, Madison, WI-USA) in a protein:trypsin ratio of 50:1 (w/w) ...
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bioRxiv - Cancer Biology 2024Quote: ... the fusion proteins were expressed using the cell-free transcription and translation system (Cat. # L4140, Promega) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... HEK293TN cells were seeded in 6-well plates and transiently transfected with plasmid of choice using FuGene transfection reagent (Promega) and OptiMem (Gibco) ...
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bioRxiv - Neuroscience 2021Quote: ... Lysates were loaded in triplicate to 96-well microtiter plates and luminescence was measured in a GloMax 96 microplate luminometer (Promega). Gene expression was measured by firefly luciferase activity (LAR II ...
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bioRxiv - Cancer Biology 2021Quote: ... The 96-well plate was then allowed to equilibrate at room temperature for 30 minutes followed by addition of Cell Titer Glo (Promega) to each well according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... S2-CP8 cells were then transiently transfected with both mRFP-FKBP-5-ptase-dom and PM-FRB-CFP (0.5 μg/well of a 6-well plate for each vector) with ViaFect (Promega Corp.). After 24 h culture ...
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bioRxiv - Cell Biology 2020Quote: ... 20 µL lysates were loaded into a 96 well plate and luminescence following administration of 50 µL Luciferase Assay Reagent II (Promega) and 50 µL Stop & Glo Reagent (Promega ...
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bioRxiv - Neuroscience 2021Quote: ... coated 12-well plate and transfected the next day using the Fugene 6 transfection reagent and according to the manufacturer’s directions (Promega E2691). The same amount of plasmid was transfected per condition ...
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bioRxiv - Molecular Biology 2021Quote: ... Cytotoxicity on mock infected plates was determined using the Promega CellTiter-Glow Luminescent Cell Viability Assay (Promega, Madison, Wisconsin, USA). Half-maximal inhibitory concentration (IC50 ...
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bioRxiv - Developmental Biology 2020Quote: ... neural stem / progenitor cells were seeded in 96-well coated plates (1×104 cells per well) and RealTime-GloTM MT Cell Viability Assay (Promega) was used with the continuous-read protocol at 0 ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated 1:1 for 5 minutes in the dark in opaque 96 well plates with NanoGlo Substrate freshly diluted 1:50 in NanoGlo Buffer (Promega). Luminescence was measured on a GloMax 96 Microplate Luminometer.
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bioRxiv - Biophysics 2022Quote: ... The reaction was further incubated for 60 min at room temperature prior to measuring absorbance with a plate-reading luminometer (GloMax, Promega). Samples were correlated to phosphate concentration standard controls ...
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bioRxiv - Biochemistry 2019Quote: JAK2-deficient ϒ2A human fibrosarcoma cells were transfected with different human JAK2-hemagglutinin (HA) constructs in pCIneo vector (100 ng per 12-well plate well) with FuGENE HD (Promega). After 48 hours ...
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bioRxiv - Molecular Biology 2019Quote: ... WT and m6A-mut expressing cells in 24-well plates were infected with the indicated virus (MOI 1) and dual luciferase assay (Promega) was performed at 48 hours post-infection according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... purified by ethanol precipitation and co-transfected into Huh7 cells in 6-well plates (90 ng reporter, 10 ng pcDNA-Blast) using FuGENE 6 transfection reagent (Promega). Cells were selected with blasticidin (0.2 μg/mL ...
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bioRxiv - Biochemistry 2019Quote: ... cells were grown in 96-well polyD-lysine coated black FLIPR plates and transfected using FuGENE HD (Promega, Madison, WI) as the transfection reagent according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Luciferase activity was quantified by transferring 20 μL of lysed cells to a 96-well plate and adding 50 μL of luciferase assay reagent (Promega). Luminescence was measured on a Perkin-Elmer Victor II plate reader ...
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bioRxiv - Microbiology 2021Quote: ... NF54-HGL parasites were treated with 3× IC50 for two weeks in two independent experiments and cultures were monitored by luminescence readout on the BioTek Synergy 2 Plate reader using ONE-Glo reagent (Promega). Whole-genome sequencing was performed to test for single nucleotide polymorphisms (SNPs ...
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bioRxiv - Bioengineering 2021Quote: ... Luminescence was measured with a FluoStar Omega plate reader (BMG Labtechnologies) and treated with the protocol and reagents of the Dual-Luciferase Reporter Assay System (Promega). All reagents were used accordingly to the manufactures protocol.
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bioRxiv - Bioengineering 2020Quote: ... Luminescence from the activity of luciferase was measured 48 h post-infection using a TECAN infinite M200 pro plate reader after applying Bright-Glo reagent (Promega) on cells.
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bioRxiv - Neuroscience 2019Quote: ... in wells of a 6-well plate and transfected 24 hours later with 500-800 ng plasmid DNA using FUGENE 6 (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... and 50 μl of diluted media added to a white 96 well plate (CLS3610) before the addition of 50 μl of Lactate/Glucose detection reagent (Promega). Plates were incubated for 1 h at RT and luminescence recorded (Glowmax Luminometer ...
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bioRxiv - Cancer Biology 2020Quote: ... The cell lysates were transferred to a 96-well black plate and then an equal volume of Luciferase assay reagent (Promega) was added ...
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bioRxiv - Biochemistry 2021Quote: ... and firefly luciferase expression was measured on a Berthold Centro LB 960 plate luminometer using D-luciferin as a substrate (Promega). Percentage of infectivity was calculated as the ratio of luciferase readout in the presence of mAbs normalized to luciferase readout in the absence of mAb ...
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bioRxiv - Microbiology 2020Quote: ... the plate was incubated at room temperature for 10 minutes to stabilize luminescent signal before luminescence was recorded on a GloMax plate reader (Promega).
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bioRxiv - Microbiology 2021Quote: ... Plates were incubated for 30 minutes in the dark before being read at 405 nm on a GloMax Explorer (Promega).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... were seeded in a 6-well plate and transfected with 750 ng of each of the AT1R-TANGO plasmids using FUGENE 6 Transfection (PROMEGA). The following day ...
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bioRxiv - Microbiology 2021Quote: ... The cells were transferred to white- walled 96-well plates and processed using the Bright-Glo Luciferase Assay System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... lysates were transferred into white 96-well plates and FLuc activity was measured using a commercial substrate (Beetle-Juice, PJK; Luciferase Assay System, Promega) and a plate luminometer (Hidex Sense Plate Reader ...
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bioRxiv - Microbiology 2020Quote: ... 2×105 HeLa-HA cells were seeded per well of a six-well plate and transfected using Fugene-HD transfection reagent (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... Two Days after infection media was removed from the cells and at least 50μl of the CellTiter-Glo® Reagent was then quickly added to all wells in the luminometer plate and the luciferase reading was quantified with a Veritas luminometer (Promega). The cytopathic effect was quantified by the CellTiter-Glo Luminescent Cell Viability Assay system (Promega ...
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bioRxiv - Cancer Biology 2020Quote: HF3016 cells were plated in individual wells of a 96-well plate and viable cells were quantified using CellTiter-Glo 3D Cell Viability Assay (Promega) in triplicate wells as per manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... mass ratio) into LentiX cells grown to approximately 80% confluency in 6-well plates using FuGENE HD Transfection Reagent (Promega) per manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... 140 mM NaCl and 1% Triton X-100) and lysates used to assay luciferase activity in 96-well plates using the Dual-Luciferase Reporter Assay (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... 25,000 leukemia cells in 250µL of drug-containing medium were seeded in a 48-well plate and viability was assessed every 4 days by using the CellTiter-Glo Luminescent Cell Viability assay (Promega). For RNA-Seq and ChIP-Seq experiments ...
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bioRxiv - Cell Biology 2022Quote: ... The cell plates were incubated at 37°C for 48-60 hours before 50 μL of CellTiter-Glo reagent (Promega) was added ...
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bioRxiv - Microbiology 2022Quote: ... The expression of firefly luciferase was measured on a Berthold Centro LB 960 plate luminometer using D-luciferin as a substrate (Promega). The percentage of neutralization was calculated as the ratio of the reduction in luciferase readout in the presence of mAbs normalized to luciferase readout in the absence of mAb ...
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bioRxiv - Biophysics 2022Quote: ... mass ratio) into LentiX cells grown to approximately 80% confluency in 6-well plates using FuGENE HD Transfection Reagent (Promega) per manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... the plates were equilibrated to room temperature and the culture media was replaced by the Nano-Glo HiBiT extracellular reagent (Promega), which was prepared according to the manufacturer’s protocol ...