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Citations for Promega :
1501 - 1550 of 5634 citations for Retinol Binding Protein RBP Multi format ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: HeLa cells were cultured on 12-well plates and transfected with the indicated plasmids using Viafect (Promega, Madison, WI). After expression for 24h ...
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bioRxiv - Molecular Biology 2021Quote: ... sphere cells growing in the low attachment plates were quantitated by CellTiter-Glo luminescent cell viability assay (Promega, G7571) using a SpectraMax M5 Multi-Mode Microplate Reader (Molecular Devices) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Cells were seeded at a density of 50,000-100,000 cells per well in quadruplicate wells of a 96-well plate in medium supplemented with 0.5 mM beetle luciferin (Promega), and drugs were prepared in water and added at the concentrations indicated in the figures and legends ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... HEK293T cells in 12-well plates were co-transfected with 0.05 µg each of GLP-1R-SmBiT and βarr2-LgBiT plasmid (Promega) plus 0.9 µg pcDNA3.1 ...
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bioRxiv - Synthetic Biology 2021Quote: Cells for were transferred to opaque-bottomed white plates (Grenier-Bio #655073) and then assessed for luminescence (Promega #E1501) per manufacturer’s protocol ...
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bioRxiv - Bioengineering 2020Quote: ... Samples were mixed by shaking the plates after which luminescence was recorded with a GloMax Explorer Model GM3510 (Promega) 10 min after adding the reagent.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 25 µL of the lysates were transferred to a white plate and mixed with 50µL of luciferase assay reagent (Luciferase assay system, Promega). We immediately measured the luciferase activity with a Tecan SPARK multifunctional microplate reader (Tecan ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmids were transfected into HeLa cells for 24 h (2 μg of cDNA/9.6 cm2 plate) using Fugene (Promega). For gene silencing ...
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bioRxiv - Molecular Biology 2022Quote: ... Fresh bacmid DNA was transfected into Sf9 cells at 0.5×106 cells/mL in 6-well cell culture plates using FuGene HD (Promega) according to the manufacturer’s protocol (final concentration 10 μg/mL) ...
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bioRxiv - Biophysics 2022Quote: Plates were left to equilibrate at room temperature for 20 minutes before addition of NanoGlo live cell substrate (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Plates were then equilibrated to room temperature and lysed by the addition of Bright-Glo reagent (100 µL; Promega) to each well ...
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bioRxiv - Neuroscience 2022Quote: ... and placed in 96-well plates (white with a clear flat bottom) containing Nano-Glo Luciferase Assay Reagent (Promega) diluted 1000-fold in PBS at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... Organoids from 2 wells of a 24 wells plate were collected in 500μl of cell lysis buffer and processed using RealiaPrep RNA Cell Miniprep System (Promega), according to manufacturer’s instructions.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 25 µL of the lysates were transferred to a white plate and mixed with 50µL of luciferase assay reagent (Luciferase assay system, Promega). We immediately measured the luciferase activity with a Tecan SPARK multifunctional microplate reader (Tecan ...
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bioRxiv - Microbiology 2020Quote: ... 48 hours after transfection into 96 well plates in triplicates and cell number was measured by MTT assay (Promega) at 4 hours after addition of tetrazolium dye.
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bioRxiv - Cell Biology 2019Quote: ... cells were grown on Precision cover glass in 24-well plates or into glass bottom imaging dishes and transfected with 0.5 μg DNA construct complexed with Fugene6 (Promega) following the manufacturer’s instruction ...
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bioRxiv - Immunology 2021Quote: ... Luciferase activity was measured on a Berthold Centro LB 960 plate luminometer using D-luciferin as a substrate (Promega). The half maximal inhibitory concentrations (IC50 ...
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bioRxiv - Molecular Biology 2019Quote: ... Plasmids were recombined into 80,000 U-2 OS cells in a 24 well plate using 1.5 uL of FuGENE6 (Promega E2691) in 100 uL OPTIMEM (Fisher Scientific 31985070 ...
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bioRxiv - Biochemistry 2021Quote: ... per well of a 96-well plate 4×104 Drosophila SR2+ cells were seeded and transfected using Fugene (Promega) according to the manufacturer’s instructions with the following plasmids ...
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bioRxiv - Microbiology 2020Quote: ... Luciferase activity was measured on a Berthold Centro LB 960 plate luminometer using D-luciferin as a substrate (Promega). The percentage of infectivity was calculated as ratio of luciferase readout in the presence of mAbs normalized to luciferase readout in the absence of mAb ...
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bioRxiv - Immunology 2020Quote: ... Luciferase activity was measured on a Berthold Centro LB 960 plate luminometer using D-luciferin as a substrate (Promega). The percentage of infectivity was calculated as ratio of luciferase readout in the presence of mAbs normalized to luciferase readout in the absence of mAb ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was transferred into a fresh 384-well assay plate containing NanoGlo Luciferase Assay Substrate (Promega, Madison, WI), and luciferase intensity was measured by EnSpire Plate Reader (PerkinElmer ...
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bioRxiv - Biochemistry 2022Quote: ... the D10ACas9 nickase and the HDR template on 6-well plates at 70% confluency using FuGene® HD (Promega). Cells were allowed to recover for 7-10 days (splitting if necessary ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell viability was assayed with CellTiter-Glo according to the manufacturer’s protocol using a Spectramax M5 plate reader (Promega).
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bioRxiv - Cancer Biology 2022Quote: ... and the viability of the cancer cells was assessed by measuring luminescence with a GloMax Discover plate reader (Promega) or an IVIS Lumina III XR (Perkin-Elmer ...
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bioRxiv - Developmental Biology 2022Quote: ... into a 10 cm plate of HEK293T cells at 60-70% using Fugene 6 transfection reagent (Promega, Cat #E2691). Media containing lentiviral particles were collected at 24 ...
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bioRxiv - Genomics 2022Quote: MM001 and MM047 were seeded in 24-well plates and transfected with 400 ng pGL4.23-enhancer vector + 40 ng pRL-TK renilla vector (Promega) with Lipofectamine 2000 (Thermo Fisher Scientific) ...
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bioRxiv - Synthetic Biology 2022Quote: ... Samples were then transferred to white 96-well plates and 50 μL of Bright-Glo Luciferase Assay Reagent (Promega) was added and mixed by manual orbital agitation ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Media was removed by spinning the inverted plates at 150g for 30 seconds followed by addition of BrightGlo (Promega) for quantification of P ...
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bioRxiv - Molecular Biology 2022Quote: HEK-293T cells were plated in a 24-well plate at 400,000 cells per well before being transiently transfected with pGL3-control (Promega), a construct constitutively expressing a firefly luciferase ...
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bioRxiv - Cell Biology 2022Quote: ... were seeded onto 6-well plates coated with 0.1 mg ml−1 poly-L-lysine and transfected using FuGENE HD transfection reagent (Promega) with a mixture of third-generation lentiviral backbone vector pJHUMCS that encodes BDNF-pHluorin ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were lysed in plate and luciferase activity was measured using Luciferase 1000 Assay System (Cat # E4550, Promega, WI).
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bioRxiv - Molecular Biology 2023Quote: ... fresh bacmid DNA was transfected into Sf9 cells at 0.5×106 cells/mL in 6-well cell culture plates using FuGene HD (Promega) according to the manufacturer’s protocol (final concentration 10 μg/mL) ...
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bioRxiv - Systems Biology 2023Quote: ... Plates were incubated at 37°C for 96 hours and lysed using CellTiter-Glo Reagent (Promega, catalog. no. G7570). Luminescence was measured using a PHERAstar FS instrument and growth inhibition was calculated relative to DMSO-treated wells.
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bioRxiv - Microbiology 2023Quote: ... Sister plates were exposed to drug but not infected to gauge cytotoxicity using a CellTiter-Glo assay (CTG, Promega) at 48 hours post treatment ...
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bioRxiv - Neuroscience 2023Quote: ... Tg(elavl3:EGFP)knu3 incross were individually placed in wells of 24-well plates in water containing 0.5 mM beetle luciferin (Promega). From ZT14 (the light to dark transition ...
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bioRxiv - Bioengineering 2023Quote: ... Lysates (20 mg per well) were assessed in 96-well plates using 90 μl reconstituted Luciferase Assay Substrate (Promega) according to the manufacturer’s directions ...
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bioRxiv - Cell Biology 2023Quote: ... cells were plated on culture plates and transfected the next day with DNA constructs using FugeneHD reagent (#E2311, Promega), according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2023Quote: ... Bacteria were plated onto pre-warmed kanamycin resistance LB agar plates in the presence of X-gal (Promega, #V3941) for blue/white screening ...
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bioRxiv - Immunology 2023Quote: ... RH5ΔNC-specific B cells were identified as live CD19+ IgG+ RH5ΔNC-APC+ RH5ΔNC-PE+ cells and single cell sorted into 96-well plates containing 10µL/well lysis buffer (10mM Tris [T3038, Merck], 1 unit/mL RNasin Ribonuclease Inhibitor [N2515, Promega]) and frozen at -80 °C.
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bioRxiv - Immunology 2023Quote: ... Bacterial growth was followed for 24h or 18h with OD600nm measurements every 30 min using GloMax Plate Reader (Promega). Anaerobia was maintained with a O2 less sealing film (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: HFF or HEK293 cells (3 × 104 cells per well of a 24-well plate or 1 × 105 cells per well of a 6-well plate) were reverse transfected with 0.5 μg of pCW57-CMV-KDEL-mCherry-GF using Fugene HD (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Plates were then incubated at 37°C overnight before luciferase activity was quantified using the Luciferase Assay System (Promega) on SoftMax Pro v.7.0.2 (Molecular Devices) ...
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bioRxiv - Microbiology 2023Quote: ... OD600nm was monitored every hour at 37°C for 24 h by using a GloMax Explorer plate-reader (Promega).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 105 HEK293T cells were seeded into 24-well plates and co-transfected with pGL4.10-based plasmids and pRL-SV40 Renilla luciferase plasmid (Promega). After 48 hours ...
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bioRxiv - Molecular Biology 2024Quote: Cells were seeded in white 96-well plates and incubated with the RealTime-Glo MT Cell Viability Assay (Promega). Luminescence was detected using a GloMax-96 instrument (Promega).
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bioRxiv - Genomics 2021Quote: ... was extracted from tissue samples using a commercial kit (Wizard R Genomic DNA Purification Kit, Promega) following manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: ... pGEM®-T kit from Promega were used ...
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bioRxiv - Immunology 2019Quote: ... using a luciferase assay kit (Promega). Results were normalized to β-galactosidase activity (Galactostar ...
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Proteomics of broad deubiquitylase inhibition unmasks redundant enzyme function to reveal substratesbioRxiv - Biochemistry 2020Quote: ... with the TNT kit (Promega: L1770). Each substrate was amplified with primers by PCR to allow T7-dependent transcription of the PCR product or transcribed directly if plasmids contained a T7 promoter ...