Labshake search
Citations for Promega :
1901 - 1950 of 5951 citations for Retinol Binding Protein RBP Multi format ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2023Quote: A mixture of 10 ng of biotinylated BSA digest and 5 µg of K562 cell digest (Promega) was added to streptavidin beads (10 µL of suspension used ...
-
bioRxiv - Physiology 2023Quote: ... Each 10 µl PCR reaction consisted of 5 µl of GoTaq® qPCR Master Mix (Promega Corporation), 0.5 µl of 10 µM forward primer ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... 0.5 µg (HEK293A, HEK293T, HEK293AΔARRB1/B2) or 2 µg (HEK293AΔGs, HEKS293AΔGi) Glo-22F cAMP plasmid DNA (Promega) and 2 µg MOCK DNA (HEK293A ...
-
bioRxiv - Developmental Biology 2023Quote: ... 16/32-cell embryos were co-injected with 5 pg of pRL-TK:renilla luciferase plasmid (Promega E2241) + 80 pg of the pGL4.23 luc2/miniP HumanPDX1 enhancer:firefly luciferase plasmid (wt or 6RFX motif mutant ...
-
bioRxiv - Cancer Biology 2023Quote: ... day 5 and day 7 for each condition using the CellTiter-Glo (CTG) luminescence-based assay (Promega). Diluted CTG reagent (100 uL 1:4 CTG reagent to PBS ...
-
bioRxiv - Molecular Biology 2023Quote: The reaction mixture (10 μl) was prepared with 5 μl of rabbit reticulocyte lysate nuclease-treated (Promega), 2 μl of H2O ...
-
bioRxiv - Plant Biology 2021Quote: ... Genomic DNA was extracted using a DNA extraction kit (Wizard Genomic DNA Purification Kit; Promega Corp, Madison, WI). Genomic DNA was extracted from 1-week old bacterial sub cultures by collecting a loop of bacteria with a sterile pipette tip and inoculating a 0.2 ml PCR tube containing 15 μl of Molecular Grade Water and ran in a thermal cycler for 15 minutes at 95 °C.
-
bioRxiv - Plant Biology 2023Quote: ... multiflorum samples using a commercial DNA extraction kit (Wizard HMW DNA Extraction Kit, Promega Corporation, Madison, WI, USA).
-
bioRxiv - Cell Biology 2020Quote: ... Proteins were renatured and assayed according to the Nano-Glo® In-Gel Detection System protocol (Promega). Light produced by NLuc was captured using a Chemidoc MP Imager (Biorad).
-
bioRxiv - Genetics 2021Quote: ... protein samples were digested overnight at 37°C with 125 ng modified porcine trypsin (Trypsin Gold, Promega). Peptides were extracted under acidic conditions and dried out using a speedvac concentrator ...
-
bioRxiv - Microbiology 2021Quote: Peptides from cell-surface/biofilm-ECM-associated proteins were released via a Trypsin/Lys-C mix (Promega) at a final concentration of 50 ng µL-1 ...
-
bioRxiv - Molecular Biology 2022Quote: ... supernatant was collected and purification was done via His-tag using HisLink Protein Purification Resin (V8823, Promega) according to manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2020Quote: In vitro transcription/translation assays were carried out in a S30 T7 HighYield Protein Expression System (Promega). The full lenght Hrnpk cDNA template was cloned from total RNA obtained from GH4C1 cells and transferred to a pET28a Escherichia coli T7 expression vector (Novagen ...
-
bioRxiv - Cell Biology 2019Quote: ... FLAG-GABARAPL2 were generated by subcloning coding sequences for each protein into pCI-neo vector (Promega, E1841) with N-terminal FLAG tag ...
-
bioRxiv - Biochemistry 2019Quote: ... N-glycans were released from the membrane-bound protein using 2 U PNGase F (Promega, WI, USA) with overnight incubation at 37°C ...
-
bioRxiv - Systems Biology 2019Quote: ... and enzymatic protein hydrolysis was initiated by the addition of trypsin (Sequencing Grade Modified Trypsin, Frozen, Promega) at a ratio of 1:50 ...
-
bioRxiv - Cancer Biology 2019Quote: ... Protein digestion on beads was carried out using a ratio 1:50 sequencing grade modified trypsin (Promega): protein extract in 100 mM ammonium bicarbonate at 37 °C overnight ...
-
bioRxiv - Biochemistry 2019Quote: ... The slices were washed and dried before digestion of proteins with 20 ng/μl of trypsin (Promega) or 12 ng/μl of chymotrypsin (Roche ...
-
bioRxiv - Bioengineering 2021Quote: Target proteins were in-vitro expressed using rabbit reticulocyte lysate (TNT-coupled reticulocyte system; Promega; #L4600, #L4610) supplemented with either 35S-methionine/S-L-cysteine mix (PerkinElmer ...
-
bioRxiv - Genetics 2019Quote: ... The resulting BMP15 protein was revealed using Transcend non-radioactive translation detection system with chemiluminescent method (Promega) after reducing SDS-PAGE on a gradient (4-15% ...
-
bioRxiv - Plant Biology 2020Quote: ... The supernatant from each sample containing precisely 10 mg of protein was digested with Trypsin Gold (Promega) at 1:50 enzyme-to-substrate ratio ...
-
bioRxiv - Systems Biology 2021Quote: ... proteins were precipitated using acetone and digested using LysC (Wako sequencing grade) and trypsin (Promega sequencing grade), as described in (Buczak et al ...
-
bioRxiv - Microbiology 2019Quote: Cell-free translated His-VqmA was produced using the S30 T7 High-Yield Protein Expression System (Promega) according to the manufacturer’s protocol with the following amendments ...
-
bioRxiv - Cancer Biology 2022Quote: ... Proteins were digested in-gel using trypsin (Gold Promega, 1 μg per band, overnight at 30°C,) as previously described (Thouvenot et al. ...
-
bioRxiv - Molecular Biology 2022Quote: ... and proteins were digested overnight with a solution of sequencing-grade trypsin (Promega, San Luis Obispo, CA) in 50 mM TEAB at a 1:50 (wt:wt ...
-
bioRxiv - Neuroscience 2022Quote: ... The samples were then incubated with 1:50 enzyme to protein ratio of sequencing-grade trypsin (Promega) overnight at 37 °C ...
-
bioRxiv - Molecular Biology 2022Quote: ... and proteins were digested overnight with a solution of sequencing-grade trypsin (Promega, San Luis Obispo, CA) in 50 mM TEAB at a 1:50 (wt:wt ...
-
bioRxiv - Cancer Biology 2022Quote: ... The proteins were then digested in situ in the column overnight using 4μg of trypsin (Promega, V5111) per column at 37C on a rotor ...
-
Herpes simplex virus 1 entry glycoproteins form stable complexes prior to and during membrane fusionbioRxiv - Microbiology 2022Quote: ... and Sm- and Lg-BiT plasmids for tagging proteins of interest were purchased from Promega (Madison, WI) [48] ...
-
bioRxiv - Plant Biology 2022Quote: ... Protein expression was confirmed by Western blot with Anti-Halo Tag monoclonal antibody (1:2000) (G9211, Promega).
-
bioRxiv - Cell Biology 2022Quote: ... SDS was removed and proteins were first digested with Lys-C (Wako) and subsequently with Trypsin (Promega) with an enzyme to protein ratio (1:50) ...
-
bioRxiv - Molecular Biology 2021Quote: ... Proteins were in vitro translated using TnT Quick Coupled Transcription/Translation System (Promega, USA, Cat. No. L1170) according to the manufacturer’s protocol.
-
bioRxiv - Neuroscience 2020Quote: ... Protein digestion was performed overnight at 37 °C in a shaking incubator using 0.12 μg trypsin (Promega) at an enzyme to protein ratio of 1:50 (protein content was calculated using independent samples) ...
-
bioRxiv - Microbiology 2021Quote: ... SDS was removed according to previous work (52) and the proteins were proteolytically digested with trypsin (Promega) and purified using OMIX C18 Mini-Bed tips (Agilent Technologies ...
-
bioRxiv - Neuroscience 2022Quote: ... The samples were then incubated with 1:50 enzyme to protein ratio of sequencing-grade trypsin (Promega) overnight at 37 °C ...
-
bioRxiv - Cell Biology 2022Quote: ... IVT reactions were done with the TNT® SP6 High-Yield Wheat Germ Protein Expression System (Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Systems Biology 2019Quote: ... Proteins were digested on-filter (16 hr, 37 °C) with sequencing-grade modified trypsin (50:1, Promega). Proteolysis was terminated and peptides eluted with 20 μL of 10% trifluoroacetic acid followed by centrifugation (13,000 ×g ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were selected by detection of HiBit protein using Nano Glo HiBiT Lytic Detection System (N3030, Promega). pX330 was a kind gift from Dr ...
-
bioRxiv - Plant Biology 2021Quote: ... The entire protein region of the gel was excised and subjected to in-gel trypsin (Promega V511A) digestion after reduction with DTT and alkylation with iodoacetamide ...
-
bioRxiv - Physiology 2020Quote: ... proteins were digested by incubation with sequencing-grade modified trypsin (1/50, w/w; Promega, Madison, Wisconsin) for 12 h at 37°C ...
-
bioRxiv - Plant Biology 2020Quote: ... Protein for MUG and LUC assays was extracted on ice in 1x passive lysis buffer (PLB: Promega). MUG assays were performed by adding 40μl of protein extract to 100μl of MUG assay buffer (2mM MUG ...
-
bioRxiv - Molecular Biology 2020Quote: ... protein samples were treated with Endoprotease Lys-C (Wako) and Trypsin (Trypsin Gold Mass Spec Grade; Promega). Peptide samples were desalted by OMIX C18 pipette tips (Agilent Technologies ...
-
bioRxiv - Microbiology 2021Quote: ... proteins were digested overnight with sequencing-grade porcine trypsin (1:25, w/w, Promega, Fitchburg, MA, USA). The resulting vacuum-dried peptides were resuspended in water containing 0.1% (v/v ...
-
bioRxiv - Genetics 2019Quote: ... proteins were digested overnight with sequencing-grade porcine trypsin (1:25, w/w, Promega, Fitchburg, MA, USA). The resulting vacuum-dried peptides were resuspended in water containing 0.1% (v/v ...
-
bioRxiv - Evolutionary Biology 2021Quote: Thirty µg of the heterologously expressed proteins were deglycosylated with endoglycosidase H following the manufacturer’s protocol (Promega GmbH ...
-
bioRxiv - Biochemistry 2021Quote: ... The proteins were digested with 1 μg lysyl endopeptidase (LysC) (FUJIFILM Wako) and 1 μg trypsin (Promega) overnight at 37°C on a shaking incubator ...
-
bioRxiv - Molecular Biology 2021Quote: ... cells were transfected with 50 ng of plasmids encoding the HiBiT-tagged proteins using FuGENE HD (Promega). Cells were trypsinized (Gibco ...
-
bioRxiv - Cell Biology 2021Quote: Proteins labeled with [35S]-methionine were produced by coupled transcription and translation reactions (SP6 or T7, Promega). Translation products were partially purified by ammonium sulfate precipitation (Bardwell & Shah ...
-
bioRxiv - Cell Biology 2020Quote: ... bound proteins were alkylated and digested with endopeptidase Lys-C (Wako) for 3 hours and trypsin (Promega) on beads overnight at 37°C.
-
bioRxiv - Immunology 2021Quote: ... and used to generate 35S-methionine labelled AIM2 protein by in vitro transcription and translation (IVTT) (Promega). Immunoprecipitations (IP ...