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Citations for Promega :
1851 - 1900 of 5951 citations for Retinol Binding Protein RBP Multi format ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 40,000 cells per well were seeded into poly-D-lysine-coated 96-well plates (Brand, 781965) and the Halo-ligand was added (1:2,000; Promega, G980A). For each transfection ...
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bioRxiv - Cancer Biology 2023Quote: Cells were plated at a density of 1*10^6 per 10cm plate and transfected on the following day using Fugene HD (Promega, Madison ...
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bioRxiv - Bioengineering 2023Quote: For luciferase expression 20 uL of undiluted sample was loaded onto a black 96 well-plate then 100uL luciferin solution (Promega) added immediately before reading on a luminometer (Wallac) ...
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bioRxiv - Bioengineering 2023Quote: ... The day after the plates were pre-cooled (RT, 15’) supplemented with luciferase substrate (Bright Glo Luciferase Assay System, Promega) incubated (RT ...
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bioRxiv - Biochemistry 2023Quote: Approximately 0.1 million cells/well of a 12-well plate were seeded with HEK293T cell line and transfected after 70% confluency using a Lipofectamine reagent (Promega). For the cycloheximide assay ...
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bioRxiv - Molecular Biology 2023Quote: ... Equal amounts of the lysate were transferred into two Microtiter plates (one for Renilla and the second for Bright Glo (Promega)) ...
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bioRxiv - Immunology 2023Quote: ... The reaction was stopped by KPL TBM Stop Solution (SeraCare, #5150-0021) and plates were read at 450nm on GloMax Explorer Multimode Microplate Reader (Promega).
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bioRxiv - Bioengineering 2023Quote: ... 2000 transfected cells were replated into white 384-well tissue culture plates in the presence or absence of 1 μM HaloTag NanoBRET-618 Ligand (Promega) and incubated overnight at 37 °C ...
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bioRxiv - Bioengineering 2023Quote: ... The fluorescence intensity of each well and empty controls was measured at 520 nm excitation on a Glomax plate reader (Promega). The average blank control values for each plate were subtracted from individual fluorescence intensity values and weekly measurements were normalized to the initial reading.
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bioRxiv - Cancer Biology 2023Quote: 500 cells/well were seeded in 96-well flat-bottom plates in 100 ml medium and an equal volume of the CellTiter-Glo Luminescent Assay Reagent (#G7571; Promega) added at the indicated time-points ...
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bioRxiv - Cancer Biology 2023Quote: ... the plates were allowed to equilibrate to room temperature for 30 minutes and 25 μL of Cytotox Glo solution (Promega) was added to the wells using a Wellmate plate filler ...
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bioRxiv - Microbiology 2023Quote: ... 8 × 105 HEK293T cells/well were seeded into 6-well plates and transfected the next day with ViaFect (Promega, E4981), using either 4 μg of plasmid expressing the G protein or 2 μg of plasmid expressing HA together with 2 μg of plasmid expressing NA ...
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bioRxiv - Microbiology 2023Quote: ... 50 μl were transferred into a white flat bottom 96 well plate in triplicates and 50 μl of Promega NanoGlo Luciferase assay reagent (Promega) prepared according to the manufacturer’s instructions was added to the wells ...
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bioRxiv - Cell Biology 2023Quote: ... Amounts of protein binding to M.tb were quantified by colorimetric measurements by absorbance at 450 nm using a GloMax plate reader (Version 3.0; Promega Corporation), and plotted based on absorbance values relative to the baseline (negative control ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 5 × 104 transfected cells were replated into white 96-well tissue culture plates in the presence or absence of HaloTag NanoBRET 618 Ligand (Promega) and incubated overnight at 37 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells was grown at 12-well plates for 18h and transfected with MCHR1 constructs and the cAMP biosensor GloSensor-22F (Promega) at a ratio of 3:2 ...
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bioRxiv - Cancer Biology 2023Quote: ... The following day 100 μl of each sample was transferred to a white 96-well plate and 100 μl of the the mix of Bright-GloTM Luciferase Assay (Promega) was added to each well ...
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bioRxiv - Cancer Biology 2023Quote: ... 30 μL of the cell lysate was transferred into a white 96-well plate and 30 μL of firefly luciferase substrate (Promega) was added ...
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bioRxiv - Bioengineering 2023Quote: For luciferase expression 20 uL of undiluted sample was loaded onto a black 96 well-plate then 100uL luciferin solution (Promega) added immediately before reading on a luminometer (Wallac) ...
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bioRxiv - Cancer Biology 2023Quote: ... pH 11 and the fluorescence was measured at 360 nm (ex) / 450 nm (em) using GloMax Explorer plate reader (Promega). The heparan sulfatase activity was confirmed by our recently described 6-O desulfation assay using GlcNS6S-GlcA-Glc6SNS-IdoA2S-GlcNS6S-IdoA2S-GlcNS6S-GlcA-pNP (2S2-6S4 ...
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bioRxiv - Genetics 2024Quote: ... HEK293T cells cultured in a 12-well plate were harvested and processed according to the instruction of Dual-Glo Luciferase Assay System (Promega) with minor modification ...
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bioRxiv - Developmental Biology 2024Quote: ... 20,000 cells were seeded in 12-well plates and transfected with 300ng of pCAG-Mest or pCAG-GFP control plasmid using FuGENE HD transfection reagent (Promega) per manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... NanoGlo Live Cell Assay System substrate was then added and donor emission (450/8 nm BP filter) and acceptor emission (600 nm LP filter) were immediately measured on a GloMax Discover plate reader (Promega). BRET binding signal was defined as acceptor/donor ratio ...
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bioRxiv - Cancer Biology 2024Quote: 1,000 HCECs or M158 cells were plated per well of 96-well plates and proliferation was measured over time using CellTiter Blue (Promega) and a plate reader ...
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bioRxiv - Cancer Biology 2024Quote: ... with 250 cells plated per well in 96-well flat-bottom plates in 100 ml medium and an equal volume of the CellTiter-Glo Luminescent Assay Reagent (#G7571; Promega) added at the indicated time-points ...
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bioRxiv - Microbiology 2024Quote: ... The expression of firefly luciferase was measured on a Berthold Centro LB 960 plate luminometer using d-luciferin as a substrate (Promega). The percentage of neutralization was calculated as the ratio of the reduction in luciferase readout in the presence of nanobody normalized to luciferase readout in the absence of nanobody ...
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bioRxiv - Bioengineering 2024Quote: ... 20 µL of undiluted sample was loaded onto a white 96 well-plate and the luminescence was read on a luminometer (Promega) after 100 µL of luciferin solution (Promega ...
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bioRxiv - Synthetic Biology 2024Quote: ... and then transferred to 96-well plates (white with a clear flat bottom) containing Nano-Glo Luciferase Assay Reagent (Promega) diluted 1000-fold in PBS at room temperature ...
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bioRxiv - Immunology 2024Quote: ... The plates were then incubated at 37°C overnight before the quantification of luciferase activity using the Luciferase Assay System (Promega) on SoftMax Pro v7.0.2 (Molecular Devices) ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR amplification was performed in 25 μl reactions using 5 μl X Green GoTaq Flexi Buffer (Promega), 1.5 μl MgCl2 (25mM ...
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bioRxiv - Biophysics 2020Quote: ... samples from homozygous HaloTag-TEV mice were incubated overnight with 5 μM HaloTag Oregon Green ligand (Promega) in Phosphate-buffered saline (PBS ...
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bioRxiv - Immunology 2019Quote: ... 5% CO2 for another 24 h before 20 μL of CellTiter 96 Aqueous One Solution (Promega, #G3582) was added and absorbance at 490 nm and 620 nm was measured ...
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bioRxiv - Plant Biology 2020Quote: ... using 3 - 5 μg of total RNA and 250 ng of a mixture of random hexanucleotides (Promega) and incubated for 50 min at 50°C ...
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bioRxiv - Cell Biology 2020Quote: ... alkylated with 5 mM iodoacetamide for 30 min in the dark and digested overnight with trypsin (Promega; 1 μg for cell-surface-enrichment samples ...
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bioRxiv - Microbiology 2022Quote: ... the modified region was amplified using primer SAM_Seq_Gen-23_FW (5’-GAT TTG AGG ACG ACT GGA CTG C) and SAM_Seq_Gen1116_RV (5’-GTC AAC TGA ACA ACC CCA AGG T) together with GoTaq polymerase (Promega), followed by cloning into pGEM T-easy vector system (Promega ...
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bioRxiv - Cancer Biology 2022Quote: ... and after 5 h the luciferase activity was measured using the Dual-Luciferase Reporter Assay System (Promega), according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... The embryos were then incubated in a 1:5,000 dilution of TMR HaloTag Ligand (5 mM; Promega) in Danieau’s medium ...
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bioRxiv - Microbiology 2020Quote: ... The reaction was stopped by the addition of 5.5μL of a mix containing 5% SDS and 0.25M EDTA and deproteinized with proteinase K (Promega) for 1hour at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... membranes were incubated in a 0.165 mg/ml BCIP (5-bromo-4-chloro-3-indolyl phosphate, Promega)/ 0.33 mg/ml NBT (p-nitroblue tetrazolium chloride ...
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bioRxiv - Molecular Biology 2022Quote: ... the HIV 5’ LTR from the pNL4-3 isolate (Genbank AF324493) was cloned into pGL3-Basic (Promega) via Gibson assembly (NEB 2X HiFi ...
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bioRxiv - Molecular Biology 2019Quote: ... Instrument quality control was monitored using the Promega 6×5 LC-MS/MS Peptide Reference Mix (Promega) before and after each MS experiment run ...
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bioRxiv - Genetics 2020Quote: ... fragments of varying sizes (Supplemental Table 5) were cloned into the promoter-less pGL3-basic vector (Promega) using NheI and NcoI sites ...
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bioRxiv - Biochemistry 2019Quote: ... followed by 5-fold dilution with Milli-Q water and digested with 100 μg of trypsin (Promega) overnight at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 5 μL (20x working stock of NanoBRET Tracer K5 [20 μM] in Tracer Dilution Buffer (Promega N291B)) was added to each well with the exception of the “no tracer” control wells ...
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bioRxiv - Plant Biology 2020Quote: ... 5 μg of RNA were depleted from contaminating DNA by treatment with RQ1 RNase-free DNase (Promega) and were reverse-transcribed with Superscript IV Reverse Transcriptase (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: ... Following 5 days of drug incubation cell viability was assayed using 30μl of CellTiter-Glo® (Promega). Screens were performed in technical triplicate.
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bioRxiv - Plant Biology 2022Quote: ... or with the same buffer supplemented with 5 U of calf intestine alkaline phosphatase (CIAP) from Promega. Samples were incubated at 30°C for 1 h ...
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bioRxiv - Molecular Biology 2024Quote: ... 50 ng of LHB promoter-containing reporter vector were transfected with 5 ng of pRLRenilla (#E2231, Promega), with or without 50 ng of pCMV6-NR2F2-WT or p.Arg246His and/or 5 ng of pCMX-NR5A1 ...
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bioRxiv - Genetics 2024Quote: ... then a 5 min final extension (72 °C)—was carried out with GoTaq Green Master Mix (Promega). Deletion loci from mutant flies were amplified using the genotyping primers above with four GoTaq Green PCR reactions per line ...
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bioRxiv - Developmental Biology 2023Quote: ... 16/32-cell embryos were co-injected with 5 pg of pRL-TK:renilla luciferase plasmid (Promega E2241) + 80 pg of the pGL4.23 luc2/miniP HumanPDX1 enhancer:firefly luciferase plasmid (wt or 6RFX motif mutant ...