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Citations for Promega :
1451 - 1500 of 4445 citations for Progesterone ELISA Kit 5 Whole Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Wizard Plus SV Minipreps DNA Purification System kit (Promega) was used following the Centrifugation Protocol under Quick Protocol ...
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bioRxiv - Plant Biology 2024Quote: ... using Maxwell 16 LEV Plant RNA kit (Promega, USA). For each genotype/treatment combination ...
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Comparative metagenomic profiling of seed-borne microbiomes in a landrace and a hybrid maize varietybioRxiv - Plant Biology 2024Quote: ... we used the Wizard Genomic DNA Purification Kit (Promega) followed by sequencing using the Sanger method.
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bioRxiv - Cancer Biology 2024Quote: ... Wizard Genomic DNA purification kits (Promega, Madison, WI, USA), or Quick-DNA Miniprep Kits (Zymo Research ...
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bioRxiv - Molecular Biology 2023Quote: ... using the Dual Luciferase Reporter Assay kit from Promega. Firefly luciferase activity was normalized against Renilla luciferase activity.
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bioRxiv - Microbiology 2024Quote: ... The Steady-Glo Luciferase Assay System kit from Promega was used for all luciferase assays ...
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bioRxiv - Immunology 2023Quote: ... ATP was measured with Mitochondrial ToxGlo Assay kit (Promega) and followed the manufacturer’s protocol.
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bioRxiv - Biochemistry 2024Quote: ... GTPase-GLO assay kit were purchased from Promega (V7681) (Madison ...
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bioRxiv - Cancer Biology 2024Quote: ... or the Maxwell® RSC simplyRNA Tissue kit (Promega) were utilized ...
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bioRxiv - Bioengineering 2020Quote: ... amplicons from three PCR runs for each root sample were combined (Clement et al., 1998) and then purified using a PCR purification kit (PCR Clean-up Kit; PROMEGA Inc., Wisconsin, USA).
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bioRxiv - Neuroscience 2020Quote: ... DNA and RNA were extracted from formalin-fixed paraffin-embedded (FFPE) tissue using the Maxwell 16 FFPE Plus LEV DNA Kit and Maxwell 16 LEV RNA FFPE Purification Kit (Promega, Madison, WI, USA), according to manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... membrane integrity (CytoTox-OneTM homogeneous membrane integrity assay kit: lactate dehydrogenase (LDH release) and caspase activity/ apoptosis (Caspase-Glo® 3/7 assay system kit) (all from Promega, WI, USA), as described previously17 (see Supplementary data for detailed description).
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bioRxiv - Developmental Biology 2019Quote: ... The cells transfected in 24-well plates were assayed post-transfection for relative luciferase activity using Dual-Luciferase Reporter (DLR™) Assay System (Promega Corporation, Wisconsin, United States) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: Cells were seeded at 2.5 x 104 cells/well in flat-bottomed 96-well plates and lysed with CellTiter-Glo® reagent according to the manufacturer’s instructions (Promega Corporation, Madison, WI 53711, USA). Plates were set to shake for 2 mins and incubated for 10 mins at room temperature prior to recording luminescence (integration time = 100 ms) ...
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bioRxiv - Molecular Biology 2020Quote: First strand synthesis was performed in 25 μl reaction volumes using 5 μl of MMLV-RT 5X buffer (Promega, Wisconsin, UK), 1.25 μl of dNTPs (10mM ...
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bioRxiv - Molecular Biology 2021Quote: ... Firefly luciferase activity was measured by adding 25 μL Luciferase Assay Buffer II (LARII) to 5 μL of lysate and measured immediately with the GloMax 20/20 Luminometer (Promega, E5311). Reactions were stopped and Renilla luciferase activity was measured by adding 25 μL Stop&Go reagent ...
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bioRxiv - Microbiology 2019Quote: ... proteins were firstly digested for 5 h at 37°C with 250 ng rLys-C Mass Spec Grade (Promega, Madison, USA). Samples were then diluted 4-fold with 100 mM Tris HCl pH 8.5 to reach a concentration of 2M urea and then re-incubated overnight at 37°C with 1 µg Sequencing Grade Modified Trypsin (Promega ...
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bioRxiv - Cancer Biology 2020Quote: CellTiter 96 AQueous One Solution Cell Proliferation Assay MTS 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) was used according to the manufacturer’s protocol (Promega, Madison, WI) to assess proliferation of cells cultured in 96-well plates.
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bioRxiv - Biochemistry 2019Quote: ... Plates were then incubated at 37°C and 5% CO2 for 48 hours upon which cell viability was assessed by adding CellTiter-Glo® (Promega) to the reaction ...
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bioRxiv - Cancer Biology 2020Quote: ... 25uL of reconstituted CellTiter-Glo Luminescent Cell Viability reagent was added to each well and incubated for 5 minutes (Promega, G7571). Luminescence readings were taken using the BioTek Synergy H1 Hybrid Reader ...
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bioRxiv - Microbiology 2020Quote: ... Plates were incubated at 37°C (5% CO2) for 48 h before performing CellTiter-Glo (CTG) assays as per the manufacturer’s instruction (Promega, Madison, WI). Luminescence was read on a BioTek Synergy HTX plate reader (BioTek Instruments Inc. ...
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bioRxiv - Biochemistry 2021Quote: ... The gel particles were eventually rehydrated on ice in a solution containing 5 ng/μL of trypsin (Promega, modified sequencing grade) in 50 mM NH4HCO3 ...
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bioRxiv - Microbiology 2020Quote: ... The plates were cultured for two days at 37°C with 5% CO2 after which the supernatant was removed and replaced with 50 μl Nano-Glo Luciferase Substrate (Promega, Inc.). Light emission was measured in an Envision 2103 Multi-label plate reader (PerkinElmer ...
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bioRxiv - Plant Biology 2020Quote: ... Individual reactions conducted with UGA-CPES consisted of a 10 µL mixture containing 5 µL of GoTaq Green Master Mix 2X (Promega Corporation), 0.5 µL of template DNA ...
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bioRxiv - Microbiology 2021Quote: ... The migrated cells in the lower chamber were stained with 5 μl of CellTiter 96 AQueous One Solution Cell Proliferation Assay (Promega # G3580) for 2 h at 37° C with 5% CO2 ...
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bioRxiv - Biophysics 2022Quote: ... Blots were incubated with secondary antibodies for 1h at room-temperature (1:10000 in 5% skim-milk powder, anti-rb-HRP-conjugate (Promgea, W401B)/anti-ms-HRP-conjugate (Promega, W402B)) ...
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bioRxiv - Biochemistry 2022Quote: The crRNA (5’-AAUUUCUACUGUUGUAGAUGUGAUAAGUGGAAUGCCAUGUGGG-3’) was synthesized and purified using the T7 RiboMAX™ Express Large Scale RNA Production System (Promega). The following DNA templates were used for the in vitro transcription reaction ...
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bioRxiv - Genomics 2022Quote: ... Membranes were washed in TBST and probed with anti-mouse HRP secondary antibody (Promega; 1:2,000 in 5% BSA in TBST) for 1 hour at room temperature followed by development in ECL (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... an 150 µL aliquot of the protein sample was digested with 5 µg of sequencing grade trypsin (Promega, Madison, WI, USA) overnight at 37°C.
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bioRxiv - Biochemistry 2021Quote: ... RRL were incubated for 5 min at 30°C then 10 min on ice with buffer containing 1U/μl of recombinant RNasin (Promega®), 75 mM KCl 0.5 mM MgCl2 ...
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bioRxiv - Immunology 2022Quote: ... Pseudoviruses stock were titrated by infecting 293T-ACE2 cells and luciferase activity was determined following a 44-48 h incubation period at 37°C and 5% CO2 by addition Bright-Glo Luciferase Assay System (Promega, E2650) using a microplate reader (TECAN ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... CellTiter 96 AQueous One Non-Radiactive Cell Proliferation Assay based on 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) was from Promega (Duebendorf, Switzerland). COmplete™ EDTA-free protease inhibitor cocktail was obtained from Roche Diagnostics (Mannheim ...
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bioRxiv - Microbiology 2020Quote: ... Amplifications were performed in a 50 µl final volume using 5 µl of DNA template and 2.5 units of GoTaq polymerase (Promega, Madison, WI). Final concentrations of MgCl2 ...
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The Arabidopsis F-box protein FBW2 degrades AGO1 to avoid spurious loading of illegitimate small RNAbioRxiv - Cell Biology 2021Quote: ... miR159 and U6 RNA (see Table S3) were 5’ end-labelled with [γ-32P]ATP using T4 polynucleotide kinase (PNK) (Promega).
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Revisiting the role of Toxoplasma gondii ERK7 in the maintenance and stability of the apical complexbioRxiv - Microbiology 2021Quote: ... Overnight digestion was performed at 37 °C with 5 μl of freshly prepared trypsin (Promega; 0.2 μg/μl in TEAB 0.1M). To remove RapiGest ...
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bioRxiv - Synthetic Biology 2021Quote: ... Media was again removed and replenished with 30 mL phenol-red free Opti-MEM supplemented with 5 mM CT-TAMRA (Promega, G8251), except for no CT-TAMRA control wells ...
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bioRxiv - Genetics 2021Quote: ... 5 μL of substrate was added to a final concentration of 100 nM 11S fragment and 0.5X furimazine (Promega, NanoGlo Substrate). Luminescence was measured on a ViewLux HTS reader (PerkinElmer ...
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bioRxiv - Microbiology 2020Quote: ... Cells were further cultured for 72 h at 37°C with 5% CO2 and lysed in luciferase substrate buffer (Bright-Glo; Promega, Poland). Lysates were transferred onto white 96-wells plates and luciferase levels were measured on a microplate reader Gemini EM (Molecular Devices ...
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bioRxiv - Cancer Biology 2021Quote: ... Plates were vigorously shaken at 80 rpm for 5 min prior to adding 75 µl of CellTiter-Glo 3D Reagent (Promega #G968B) to each well ...
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bioRxiv - Plant Biology 2020Quote: ... cDNA was diluted 5 folds with distilled water and used in a reaction mix composed of 1x GoTaq qRT-PCR master mix (Promega, USA) and 10 nM primers for RT-qPCR ...
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bioRxiv - Plant Biology 2020Quote: ... All PCR amplifications were assembled on ice in 25 μl reactions using 5 μl of 5X Green GoTaq buffer (Promega M3001), 0.5-1 μl of each primer (10 μM) ...
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bioRxiv - Microbiology 2022Quote: ... the PCR was performed in a total volume of 25 μl containing 5 X GoTaq® buffer (Promega, Madison, WI, USA), 2.5 mM MgCl2 ...
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bioRxiv - Microbiology 2022Quote: Various KSHV mRNA 5’-UTRs containing uORFs were cloned up stream of the Renilla luciferase gene in a psiCHECK™-2 vector (Promega) using a Gibson Assembly® Cloning Kit (New England Biolabs) ...
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bioRxiv - Immunology 2022Quote: ... DDX60 (Fw 5’- AAGGTGTTCCTTGATGATCTCC-3’ Rv : 5’ -TGACAATGGGAGTTGATATTCC-3’) as analyzed by semiquantitative PCR using the SYBR Green assay GoTaq® qPCR Master Mix (Promega) with standardized primers (Metabion) ...
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bioRxiv - Microbiology 2023Quote: ... Nanoluciferase bioluminescence was measured from the Hyp1-Nluc parasites by lysing the parasite culture 1:5 with 1x Nano-Glo Lysis Buffer (Promega, USA), and the addition of 1:1000 Nano-Glo (Promega) ...
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bioRxiv - Cell Biology 2023Quote: ... reduced with 5 mM DTT and alkylated with 20 mM acrylamide.23 The reaction was quenched by addition of 5 mM DTT and 1.5 µg of trypsin (Promega, Madison, WI) was added to the sample ...
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bioRxiv - Microbiology 2023Quote: ... The membranes were blocked with PBS containing 5% skim milk and incubated with HRP-conjugated anti-human IgG1 antibodies (Promega, #W403B) overnight at 4 °C.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 4 h later 5 µl/well of NanoBRET™ Nano-GloR Substrate (10 µl/ml in DMEM no phenol red, Promega) was added and 460 nm donor and 618 nm acceptor signals were read within 10 min of substrate addition using CLARIOstar microplate reader (Mandel) ...
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bioRxiv - Biophysics 2023Quote: DNA dT70 was diluted to ∼100 pM in buffer (50 mM Tris-acetate pH 7.7, 5 mM Mg-acetate, 50 mM Na-acetate) and SSB (Promega Corporation, USA) was supplemented in specified concentrations (Tab ...
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bioRxiv - Microbiology 2023Quote: ... The migrated cells in the lower chamber were stained with 5 μl of CellTiter 96 AQueous One Solution Cell Proliferation Assay (Promega # G3580) for 2.5 h at 37° C with 5% CO2 ...