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Citations for Promega :
1651 - 1700 of 4445 citations for Progesterone ELISA Kit 5 Whole Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2022Quote: ... 102.2 µL of 100 mM ABC was added and proteins were digested for 16 hours at 37°C using 5 µg of trypsin (dissolved in trypsin resuspension buffer, Promega, Walldorf, Germany). Tryptic digestion was stopped by addition of 2.5 µL 100% formic acid ...
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bioRxiv - Cancer Biology 2019Quote: ... Cell viability was assessed using the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) CellTiter 96 Aqueous One Solution Cell Proliferation Assay (Promega, Madison, WI) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... the samples were incubated for 5 min at room temperature with equivalent volumes of Bright-GloTM firefly luciferase substrate (Promega, Madison, WI), and the luciferase signal was measured using a TopCount NXT plate reader (PerkinElmer Life Sciences ...
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bioRxiv - Molecular Biology 2021Quote: ... cooled on ice to room temperature for 5 min and digested overnight at 37 °C with 0.5 μg of sequencing-grade trypsin (Promega Cat. No. V5111). Peptide mixtures were acidified to pH 3 with 1.5 µl 5% FA ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell viability was determined for the serially diluted cells 5 days after seeding with the Cell Titer Glo assay (Promega, cat# G7573) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA was synthesized via reverse-transcription of 2-5 μg of RNA using M-MLV Reverse Transcriptase (200 U, Promega, Wisconsin, USA), random primers (10 ng/μl ...
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bioRxiv - Cancer Biology 2022Quote: Cells were seeded on 24-well cell culture plates and transfected in triplicates with 20 ng of firefly luciferase reporter pGL3 β-catenin-activated reporter (pBAR) (19099249) and 5 ng of renilla luciferase reporter pGL4.73 hRLuc/SV40 (RLuc; Promega, WI, USA) and a membrane GFP (75 ng as control for transfection ...
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bioRxiv - Plant Biology 2022Quote: ... Approximately 2-5 ng of purified genomic DNA was spiked with 1% (w/w) of unmethylated cl857 Sam7 Lambda DNA (Promega, Madison, WI) and sheared to about 400bp using Covaris M220 (Covaris Inc. ...
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bioRxiv - Molecular Biology 2023Quote: ... DNase-treated RNA was ligated with a 5’-hydroxylated ‘Processed Start Site’ (PSS) RNA adaptor oligomers (onkh031) by RNA Ligase 1 (Promega or NEB) and then purified to remove excess oligomers (NEB Monarch RNA Cleanup kit) ...
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bioRxiv - Molecular Biology 2022Quote: ... SARS-CoV-2 5’-UTRC75G and SARS-CoV-2 5’-UTRC75G + G61C gBlocks™ DNA fragments (Integrated DNA Technologies) were cloned immediately upstream of fLuc into pGL3-fLuc plasmids (Promega, #E1751). Cells were transfected as per manufacturer protocol (Polyplus ...
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bioRxiv - Systems Biology 2022Quote: ... 102.2 µL of 100 mM TEAB was added and proteins were digested for 16 hours at 37°C using 5 µg of trypsin (dissolved in trypsin resuspension buffer, Promega, Walldorf, Germany). Tryptic digestion was stopped by addition of 2.5 µL 100% formic acid ...
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bioRxiv - Molecular Biology 2023Quote: ... a mixture of 20 ng of CD44-smBiT and 5 ng of LgBiT-MSN(T558D) plasmids was added to 75 ng of Fugene HD (Promega, Cat#2312) transfection reagent diluted in DMEM ...
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bioRxiv - Cell Biology 2023Quote: ... Samples containing 5-10 µg of protein were mixed with 0.25-0.5 µg BSA (used as an internal standard in this analysis) and cleaved with 1 µg trypsin/LysC mix (Promega, Madison, WI) using the SP3 beads protocol described in (Hughes et al. ...
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bioRxiv - Biophysics 2023Quote: HeLa cells expressing each Halo-tagged chromatin remodeler were labeled with 5 nM Janelia Fluor® 549 (JF549) Halo-tag® ligand (GA1110, Promega) for 15 min at 37 °C ...
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Photoreceptor disc incisures form as an adaptive mechanism ensuring the completion of disc enclosurebioRxiv - Cell Biology 2023Quote: ... Samples containing 20 µg of protein were mixed with 0.5 µg BSA (used as an internal standard in this analysis) and cleaved with 1 µg trypsin/LysC mix (Promega, Madison, WI) using the SP3 beads protocol described in (Hughes et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... and the HBB 5′ UTR (ENST00000335295.4) commercially generated (IDT) sequences were each inserted into the pNL1.1 NanoLuc luciferase reporter plasmid (Promega, GenBank Accession Number JQ437370) downstream of a T7 promoter using overlap-extension PCR with Q5 High-Fidelity DNA Polymerase (NEB ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated for 24-48hrs in the blocking solution containing chicken anti-human NT-4/5 antibody (10 mg/ ml, Promega, Madison, WI) or rabbit anti-human NT-4/5 (1:1000 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Bound proteins were eluted with elution buffer 1 (50 mM Tris-HCl pH 7.5, 2 M urea, 5 ng/µL sequencing grade modified trypsin (Promega, Madison, WI, USA), 1 mM DTT ...
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bioRxiv - Immunology 2024Quote: ... AP20187 dimerizer was added in cell culture media to a final concentration of 500 nM for 5 hours before taking the supernatants for LDH assay (G1780, Promega, Madison, WI). Cell death was measured on a scale with the media without cells set to 0% and cells lysed for 1 hour in 1% Triton X-100 set to 100%.
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bioRxiv - Cell Biology 2024Quote: ... 50,000 cells were seeded on 3.5 cm imaging dishes and grown to 40% confluency prior to transfection with the indicated plasmids using FuGENE HD (Promega, Madison, WI). In some cases ...
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bioRxiv - Microbiology 2020Quote: The biomass recovered from isolate incubation in broth medium was subjected to DNA extraction using the commercial kit Wizard® Genomic DNA Purification Kit (Promega Corporation, Madison, WI, USA), following the manufacturer’s recommendations ...
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bioRxiv - Synthetic Biology 2021Quote: All Plasmids were purified using the Midiprep kit (QIAGEN or Promega)
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bioRxiv - Cancer Biology 2021Quote: Dual-luciferase report assay was determined with the kit (Promega, E1910) following manufacturer’s protocols ...
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bioRxiv - Immunology 2021Quote: ... The luciferase activity were measured using a luciferase assay kit (Promega) and subtracted with the background level obtained from co-cultures in the absence of mAb.
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bioRxiv - Cell Biology 2020Quote: ... used for the Luciferase reporter assay (Steady Glow Luciferase Kit, Promega) and quantified as Relative Light Unit (RLU ...
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bioRxiv - Cell Biology 2020Quote: ... Extracts were analyzed using the Dual-Luciferase Reporter Assay Kit (Promega). The ratio of Luciferase/Renilla was calculated and normalized to experimental control (promoter in absence of experimental plasmid).
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bioRxiv - Cell Biology 2020Quote: Luciferase assays were performed with the dual luciferase assay kit (Promega). Cells were lysed using passive lysis buffer ...
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bioRxiv - Cell Biology 2020Quote: ... Luciferase expression levels were analyzed using the Dual Luciferase kit (Promega) in a DTX880 Multimode detector (Beckman Coulter).
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bioRxiv - Immunology 2021Quote: ... cDNA was synthesized with the M-MLV reverse-transcriptase kit (Promega) and diluted 1 in 5 before further application ...
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bioRxiv - Genetics 2021Quote: ... PCR were performed with the kit GoTaq® Flexi (Promega, USA) using 20 ng DNA ...
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bioRxiv - Neuroscience 2020Quote: ... and transcribing using the T7 RiboMAX RNA Production kit (Promega-P1320). Cas9 was transcribed from the plasmid pCS2-Cas9 (Addgene ...
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bioRxiv - Genomics 2021Quote: ... and Maxwell® RSC ccfDNA Plasma Kit (Promega, Leiden, the Netherlands). DNA was eluted in elution buffers provided by the kits used (QIAamp kit ...
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bioRxiv - Microbiology 2019Quote: ... DNA extraction was performed using the Maxwell Cell tissue kit (Promega). The primers ...
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TSG101 Associates with PARP1 and is Essential for PARylation and DNA Damage-induced NF-κB ActivationbioRxiv - Molecular Biology 2021Quote: Cell viability was assayed using the CellTiter-FluorTM kit (Promega, G6081) according to manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... This was purified using a PCR clean up kit (Promega, A9281). The sgRNAs were synthesized using the MEGAscript™ T7 Transcription Kit (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... Luciferase activity was measured by Luciferase Assay kit (Promega, WI, USA). The 50% neutralization titer (NT50 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The CellTiter-Glo® Luminescent Cell Viability Assay Kit (Promega, # G7570) was used as recommended in the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... with an adenosine triphosphate (ATP) content assay kit (catalog 6016949, Promega).
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bioRxiv - Molecular Biology 2020Quote: ... total RNA was reverse-transcribed using GoScript Reverse Transcriptase Kit (Promega). Second ...
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bioRxiv - Cell Biology 2022Quote: ... A549 cells were solubilized using the Dual-Luciferase Assay Kit (Promega), and luciferase activity was quantified using a microplate dual-injector luminometer (Veritas ...
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bioRxiv - Immunology 2022Quote: ... cDNA was then synthesized using the reverse transcription kit from Promega. Transcriptional levels of PD-L1 (Forward ...
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bioRxiv - Immunology 2021Quote: ... CytoTox 96 LDH-release assay kit (G1780) was purchased from Promega.
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bioRxiv - Cell Biology 2020Quote: ... The dual luciferase reporter assay kit was purchased from Promega (E1910). Dulbecco’s Modified Eagle’s Medium (DMEM) ...
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bioRxiv - Microbiology 2019Quote: ... gDNA was isolated using the Wizard Genomic DNA extraction kit (Promega). gDNA was then fragmented to 400-600 bp by sonication (Covaris E220 ...
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bioRxiv - Microbiology 2020Quote: ... and Gaussia activity was measured using a Renilla luciferase kit (Promega) on a FLUOstar Omega plate reader (BMG Labtech) ...
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bioRxiv - Biochemistry 2019Quote: ... Luciferase assays were performed using a dual-luciferase assay kit (Promega), quantified with monolight 3010 luminometer (BD Biosciences) ...
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bioRxiv - Biophysics 2019Quote: ... cDNA was transcribed from RNA (Reverse Transcriptase kit, Promega, Madison, USA) using Reverse Transcriptase (Roche ...
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bioRxiv - Cancer Biology 2019Quote: Cellular glutathione was quantified using the GSH/GSSG-Glo kit (Promega) according to the instructions provided by the manufacturer ...
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bioRxiv - Genetics 2019Quote: ... and cDNA synthesis (M-MLV RT kit, Promega Corporation, cat. #M170A) were performed according to manufacturer’s specifications [21] ...
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bioRxiv - Evolutionary Biology 2019Quote: ... DNA was extracted using the Wizard Genomic DNA purification kit (Promega). Data on genome-wide admixture ...