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Citations for Promega :
1401 - 1450 of 2189 citations for Pops Par Solution Unlabeled 2000 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: Cell proliferation assay was performed in 96 well plate using CellTiter 96® AQueous One Solution Reagent (Promega) as per the manufacturer’s protocol ...
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bioRxiv - Genomics 2019Quote: ... Alternatively, ¼ of an agarose plug was soaked in GET solution (3% 2-mercaptoethanol, 0.5x QuantiFluor® dye (Promega), 1X TBE ...
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bioRxiv - Zoology 2021Quote: ... Sections were digested in a mixture of 97% Nuclei Lysis Solution (Promega; from the Wizard DNA isolation kit) and 3% 20mg/ml Proteinase K (Promega) ...
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bioRxiv - Microbiology 2021Quote: Cellular viability was determined using non-radioactive CellTiter 96 AQueous One Solution Cell Proliferation Assay (MTS) reagent (Promega), according to the manufacturer’s recommendations29 ...
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bioRxiv - Cell Biology 2022Quote: ... Cell titer 96® aqueous one solution cell proliferation (MTS) assay (catalog no. G3580) was purchased from Promega Corporation (Madison ...
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bioRxiv - Biophysics 2019Quote: ... The sample solutions were diluted to 1 M urea with 50 mM ammonium hydrogen carbonate and trypsin (Promega) was added at an enzyme-to-substrate ratio of 1:50 ...
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bioRxiv - Biochemistry 2019Quote: ... In-column digest was performed by adding 25 µL of a solution containing 2 µg trypsin (Promega, V5111) in 50 mM TEAB pH 8.0 ...
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bioRxiv - Systems Biology 2019Quote: ... Samples were diluted to 2M urea and were digested in-solution overnight using Trypsin (Promega Corporation, Madison, WI) as previously described to derive peptides (Mosley et al. ...
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bioRxiv - Microbiology 2020Quote: ... cell viability was monitored using the CellTiter 96 Aqueous One Solution Cell Proliferation Assay (Promega, Madison, WI, USA). VeroE6 cells and Huh7.5 cells were seeded in 96-well flat-bottom plates at 10,000 and 9,000 cells per well ...
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bioRxiv - Microbiology 2021Quote: ... 40 μL of the lysate supernatant were mixed with the same volume of Nano-Glo reaction solution (Promega), incubated for 3 min at room temperature ...
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bioRxiv - Cancer Biology 2019Quote: ... Cell proliferation was assessed using the CellTiter96 Aqueous One Solution Cell Proliferation Assay (MTS) kit (Promega, Madison, WI).
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bioRxiv - Molecular Biology 2019Quote: ... dried in a Speed-Vac again and finally rehydrated with 20μL of trypsin solution [10ng/μL trypsin (PROMEGA) in 25mM NH4HCO3 and 0.01% ProteaseMAX w/v (PROMEGA)] ...
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bioRxiv - Microbiology 2021Quote: ... Cells (100 μL) were then incubated with 4 μM cheopin or Fast Break lysis solution (Promega, Madison, WI) and absorbance at 405 nm was measured for 1h using a BioTek Synergy H1M plate reader set to 28°C.
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bioRxiv - Cell Biology 2020Quote: ... and the solution was added to OMEM (75 μL) containing 6 μL Fugene HD reagent (Promega, Madison, WI). The mixture was incubated at room temperature for 10 minutes and gently added to the growth medium on the cultured cells ...
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bioRxiv - Genomics 2020Quote: ... each of the wells containing 0.5 μl of lysis solution (0.2% Triton X-100 [Roche], 0.8 units of RNasin Plus [Promega], 4 mM dNTPs [Promega] and 1 μM of barcoded oligo-dT primers [E3V6NEXT ...
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bioRxiv - Immunology 2021Quote: Cell viability was determined using CellTiter 96® Aqueous One solution according to the manufacturer’s instructions (Promega, Singapore).
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bioRxiv - Cancer Biology 2022Quote: ... and 100 μl of 50 mM ammonium bicarbonate buffer as well as 10 μl of protease solution (Promega Trypsin/Lys-C Mix ...
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bioRxiv - Cell Biology 2022Quote: ... Peptide solution was then acidified to 0.1% formic acid before adding 0.2 μg Mass Spec grade ProAlanase protease (Promega) and incubating at 37°C for a further 2 h ...
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bioRxiv - Neuroscience 2022Quote: ... cells were pelleted and resuspended in the Homogenization Solution of Maxwell® RSC simplyRNA Tissue Kit (Promega, AS1340) and stored at -80°C until quantitative PCR experiments.
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bioRxiv - Biophysics 2024Quote: ... Ninety microliters of the oocyte supernatant were added to 40 µl of luciferase-luciferin solution (Promega, Madison, WI) for assaying luciferase activity ...
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bioRxiv - Cell Biology 2023Quote: ... We then mixed the cell solution with 80 μL of NanoGlo luciferase assay reagent (Promega, Cat. No. N1110), transferred it to a 96-well plate well (PerkinElmer,Cat ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were extracted with 1XPLB solution provided in the Dual-Luciferase® Reporter Assay Kit (Promega CN: E1910) and transferred to 96-well black plate to be treated with LARII and Stop & Glo components according to protocol of the Dual-Luciferase® Reporter Assay Kit (Promega CN ...
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bioRxiv - Neuroscience 2023Quote: ... RNA was then isolated from the isopropanol/chloroform solution using the ReliaPrep RNA Cell Miniprep kit (Promega Z6010). For linear RNA depletion ...
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bioRxiv - Bioengineering 2023Quote: ... CellTiter 96 AQueous One Solution Cell Proliferation Assay (MTS) and CellTiter Blue were purchased from Promega (Madison, WI). Annexin V-Orange (4759 ...
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bioRxiv - Cancer Biology 2023Quote: ... 72-hour viability assays were performed using CellTiter 96® AQueous One Solution Cell Proliferation Assay (Promega, G3580) following manufacturer recommendations and 490nM absorbance measured using a CLARIOstar plate reader (BMG Labtech) ...
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bioRxiv - Plant Biology 2024Quote: ... benthamiana leaves were collected at 2 dpi sprayed with a solution of 1 mM D–luciferin (Promega, E1603) and 0.02% (v/v ...
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bioRxiv - Bioengineering 2024Quote: Cell viability was assessed using the CellTiter 96® AQueous One Solution Cell Proliferation (MTS) Assay (Promega, Switzerland). One day before treatment ...
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bioRxiv - Genetics 2024Quote: ... 2 µl of the extracted genome solution was used as a template for Genome PCR by GoTaq (Promega). The sequences of gw182 gene specific primer set designed around the CRISPR target site were 5’ -GAG TCC AAT TTG AGA AAC GGA GGT CA-3’ and 5’ - CTG ATC GTT TGC GCT TAA CTT CAT TAA TTC T-3’ ...
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bioRxiv - Cell Biology 2024Quote: ... which was followed by the addition of 25 μL of a 5X solution of NanoGlo substrate (Promega, #N2161). The cells were then transferred to a white non-binding surface (NBS ...
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bioRxiv - Molecular Biology 2021Quote: cDNA synthesis for gene expression analyses was performed using 500 ng RNA using Moloney murine leukemia virus (M-MLV) reverse transcriptase and random hexamer oligonucleotide (both from Promega, Southampton, UK) using 500 ng RNA in a 25 µl reaction ...
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Pluripotent stem cell SOX9 and INS reporters facilitate differentiation into insulin-producing cellsbioRxiv - Developmental Biology 2021Quote: ... cDNA samples were then diluted to 2.5-5 ng/µl and measured in a qPCR reaction with the GoTaq® qPCR Master Mix (Promega, Walldorf, Germany). All reactions were performed by a 2-step PCR in triplicates followed by melting curve analysis on a ViiA7 real-time PCR cycler (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... reverse transcription assays were performed according to the manufacturer’s instructions with 2,5 μg of total RNA in the presence of 5 μM of oligo(dT) primer (Table S3) and 25 ng/μl of Random Primers (Promega Cat#C118A) using the SuperScript™ IV Reverse Transcriptase (Invitrogen Cat#18090010) ...
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bioRxiv - Molecular Biology 2021Quote: ... in vitro synthesized C9-RNA and pcDNA-FF RNA were co-transfected at 50 ng/well each into the well by Viafect transfection reagent (Promega, Cat# E4981) as described above ...
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bioRxiv - Cancer Biology 2019Quote: ... with 200 ng of miR-211 overexpression construct or empty vector along with 5 ng of pRL-CMV plasmid vector encoding Renilla luciferase gene using FuGENE transfection reagent (Promega, Madison, WI). After 48 h incubation ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were first digested for 5 h at 37 °C with 500 ng rLys-C Mass Spec Grade (Promega, Madison, WI, USA) before being diluted 4-fold with 100 mM Tris HCl pH 8.5 to reach a concentration below 2 M urea ...
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bioRxiv - Cell Biology 2021Quote: ... The gel piece was vacuum dried and treated with sequencing grade trypsin (15 ng/μl in 25 mM ammonium bicarbonate, 1 mM CaCl2; Promega or Roche) at 37°C for 10-16 hours ...
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bioRxiv - Cell Biology 2020Quote: ... Total 500 ng RNA was used to synthesize cDNA by using Moloney-murine leukemia virus (M-MLV) reverse transcriptase (Promega, Madison, WI). Diluted cDNA was used in each quantitative reverse transcription-PCR (qRT-PCR) ...
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bioRxiv - Plant Biology 2020Quote: ... The columns were capped at the bottom and 200 µl AmBic containing 4 ng/µl of Trypsin + LysC (Promega Catalog number V5073) was added to each sample and incubated in a 37°C shaker for 16 hours ...
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bioRxiv - Microbiology 2021Quote: Protein samples were alkylated with 10 mM iodoacetamide in the dark at 37°C for 30m and subsequently digested with trypsin (Promega, 50 ng) overnight at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... A total of 200 ng total RNA was used for reverse transcription reactions to synthesize cDNA with revere transcription system A3500 (Promega, Madison, WI). Then ...
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bioRxiv - Cancer Biology 2022Quote: Cells were seeded on 24-well cell culture plates and transfected in triplicates with 20 ng of firefly luciferase reporter pGL3 β-catenin-activated reporter (pBAR) (19099249) and 5 ng of renilla luciferase reporter pGL4.73 hRLuc/SV40 (RLuc; Promega, WI, USA) and a membrane GFP (75 ng as control for transfection ...
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bioRxiv - Plant Biology 2022Quote: ... Approximately 2-5 ng of purified genomic DNA was spiked with 1% (w/w) of unmethylated cl857 Sam7 Lambda DNA (Promega, Madison, WI) and sheared to about 400bp using Covaris M220 (Covaris Inc. ...
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bioRxiv - Molecular Biology 2023Quote: ... diluted with 4 volumes of 20 mM Tris-HCl pH 8 2 mM CaCl2 prior to overnight digestion with 100 ng sequencing grade trypsin (Promega, Cat# V5111) at room temperature ...
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bioRxiv - Zoology 2022Quote: ... The mixture was diluted with 150 μL of 50 mM Tris-HCl pH8.0 and digested by adding 500 ng Trypsin/Lys-C mix (Promega, Madison, WI, USA) overnight at 37 °C ...
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bioRxiv - Genomics 2023Quote: ... traces of genomic DNA were removed by treating 600 ng of total RNA with 1 µl RQ1 RNase-Free DNase (Promega, cat# M6101) in 9 µl reaction additionally containing RQ1 DNase buffer and 1 µl of murine RNase inhibitor at 37°C for 45 minutes ...
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bioRxiv - Bioengineering 2023Quote: ... We then performed first-strand cDNA synthesis from 400 ng total RNA in 20 uL reactions using Promega GoScript Reverse Transcription Kit (Promega, catalog # A5000).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Bound proteins were eluted with elution buffer 1 (50 mM Tris-HCl pH 7.5, 2 M urea, 5 ng/µL sequencing grade modified trypsin (Promega, Madison, WI, USA), 1 mM DTT ...
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bioRxiv - Biochemistry 2023Quote: One-hundred microliters of 50 mM Tris-HCl (pH 8.0) and 500 ng of trypsin/Lys-C mix (Promega, cat. no. V5072) were added to the washed HaloTag ligand plate following the first-generation assay and mixed gently at 37 °C overnight to digest the proteins ...
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bioRxiv - Molecular Biology 2023Quote: HEK293 cells were seeded in 24-well plates at a density of 4 × 104 or 8 × 104 cells/well and co-transfected 24 hours later with 200 ng of the PsiCheck2 plasmid (Promega, cat #C8021) with the human dystrophin 3′ UTR or mutant 3′ UTR and with 50 nM miR-146a mimic (Life Technologies ...
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bioRxiv - Plant Biology 2024Quote: ... Two hundred ng RNA were used to prepare complementary DNA (cDNA) using M-MLV reverse transcriptase kit (Promega, Charbonnières-les-Bains, France) and oligo(dT ...