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Citations for Promega :
1601 - 1650 of 2189 citations for Pops Par Solution Unlabeled 2000 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Proteins were then digested in-gel by rehydrating the gel slices in trypsin enzyme solution consisting of 6ng/µl trypsin (Promega) in 50mM ammonium bicarbonate solution ...
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bioRxiv - Cancer Biology 2020Quote: Short term cell viability was assessed using the Cell Titer 96 AQeous One Solution MTS cell proliferation assay (Promega, UK), as previously described {Beeby ...
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bioRxiv - Cell Biology 2021Quote: ... cells were again washed with DPBS and incubated with 90 μL of a 1/1000 dilution of furimazine stock solution (Promega) in 0.1 % BSA/HBSS ...
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bioRxiv - Biophysics 2020Quote: ... 70 μL of PBS were added to each well followed by addition of 10 μL of a 50 μM coelenterazine-h solution (Promega). After 10-min incubation ...
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bioRxiv - Cancer Biology 2022Quote: Relative cell viability was measured by MTS assays using the CellTiter 96® AQueous One Solution Cell Proliferation Assay (Promega). Briefly ...
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bioRxiv - Molecular Biology 2022Quote: ... 5 μg of the purified RNA was further treated in solution by 5 units of RQ1 DNase in 100 μl of 1x RQ1 buffer (Promega) for 1 h at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... the culture medium was replaced with a CO2-independent medium containing 10% (vol/vol) FBS and 2% (vol/vol) GloSensor cGMP stock solution (Promega). The cells were then incubated for 2 h at 27°C in the dark ...
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bioRxiv - Neuroscience 2020Quote: RNA was isolated from pooled neurons that were stored at −80 in saline solution with 1% (v/v) Recombinant RNasin Ribonuclease Inhibitor (Promega). The RNA was extracted with the Quick-RNA micro prep kit (Zymo ...
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bioRxiv - Molecular Biology 2019Quote: Cell viability was determined 72 h after PDS or cisplatin treatment using a CellTiter 96 AQueous One Solution Cell Proliferation Assay (Promega), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... the supernatant was aspirated and gently resuspended in 500 μl of cold buffer (1x of cold Hanks’ Balanced Salt Solution HBSS, 1% nuclease-free BSA, 22.5 μl of RNasin Plus (Promega N2611) and 1/2000 DRAQ5).
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bioRxiv - Molecular Biology 2019Quote: ... Relative numbers of viable cells in each well were determined using CellTiter 96 AQueous One Solution Cell Proliferation Assay (Promega). Experiments were performed in six biological replicates ...
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bioRxiv - Microbiology 2019Quote: ... Parasites viability was assessed by direct light microscope observations and MTS colorimetric assays (CellTiter Aqueous One Solution Cell Proliferation Assay -Promega); they maintained shape and motility at 5 ...
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bioRxiv - Microbiology 2020Quote: ... Cell viability was evaluated using the MTS-based CellTiter 96® AQueous One Solution Cell Proliferation Assay kit (Promega, G3582), which determines viable cell number measuring the conversion at 490 nm of 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS ...
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bioRxiv - Biochemistry 2019Quote: The linear dynamic range for measurement of complex peptide mixtures on the Lunatic was determined on a group of eight solutions prepared from a 1.0 μg/μl stock of a commercial yeast protein digest (Promega, V7461), dissolved in loading solvent (0.1% TFA in acetonitrile/water ...
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bioRxiv - Neuroscience 2019Quote: ... The samples were then cleared by centrifugation and the supernatant was diluted (1/50) with extraction buffer and mixed with the luminescent solution (CellTiter-Glo Luminescent Cell Viability Assay, Promega). The luminescence was measured on an Infinite M200PRO plate reader (TECAN ...
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bioRxiv - Biochemistry 2021Quote: ... 5 μL of a solution that is 1 mM in each of the 20 essential amino acids (Promega, No. L4461); 20 μl of Promega S30 Premix without Amino Acids (No ...
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bioRxiv - Zoology 2021Quote: ... Polymerase chain reactions in a 15 μL solution containing 7.5 μL GoTaq® G2 Hot Start Green Master Mix (Promega), 0.4 μmol of each primer and 46 – 247 ng of genomic DNA were performed using a C1000 Touch Thermal Cylinder (BioRad) ...
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bioRxiv - Microbiology 2020Quote: ... enriched proteins were digested at 37 °C overnight on agarose beads in RapiGest SF solution (Waters, 186001861) supplemented with 0.75 μg trypsin (Promega, V511A). The reaction was stopped using 1% TFA (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... The reduced and alkylated protein lysates were diluted using 50 mM Tris-Cl to obtain a final concentration of 2 M urea in solution followed by overnight digestion with trypsin (1:1000)(Promega). Peptides were then desalted using c-18 Sep-Pack columns (Waters) ...
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bioRxiv - Biochemistry 2020Quote: ... and bound proteins were digested for ∼14 hr at 37°C in 200 μL of trypsin premix solution containing sequence grade trypsin (2 mg, Promega), urea (0.5 M in 200 mM EPPS pH 8.4) ...
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bioRxiv - Microbiology 2021Quote: ... cellular cytotoxicity was evaluated using the CellTiter 96® AQueous One Solution Cell Proliferation Assay kit using manufacturer’s instructions from Promega. Briefly ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins on the filter unit were digested with 40 μl of trypsin solution (trypsin [V5111, Promega] in 50 mM NH4HCO3) at 37°C for 18 h ...
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bioRxiv - Cell Biology 2021Quote: ... proteins were reduced by dithiothreitol and alkylated by iodoacetamide in the dark at room temperature, followed by in-solution digestion sequentially using lysyl endopeptidase (Lys-C, Wako) for 3 h and trypsin (Promega) overnight at room temperature as previously described (Paul et al. ...
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bioRxiv - Biochemistry 2022Quote: ... The proteins were rinsed with urea and ammonium bicarbonate solutions and digested overnight at 37°C using trypsin gold (Promega) at a ratio of 1:100 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Flies were placed individually in 100 ul Chelex solution (5g of Chelex mixed with 95ml TE buffer) with 5 ul proteinase K (Promega), and incubated overnight at 56°C ...
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bioRxiv - Immunology 2022Quote: ... Cell viability was assessed using a Cell Titer 96 AQueous One Solution Cell Proliferation Assay (Promega Corp, Madison, Wisconsin, USA). Absorbance measurements at 490 nm were normalized to the maximum read per cell line ...
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bioRxiv - Microbiology 2022Quote: Cell viability of different 2D cultures at different time points of infection or exposure to virus proteins or HAE supernatant was determined using the CellTiter 96 AQueous One Solution cell proliferation assay (G3582; Promega), while HTO viability was determined by the CellTiter-Glo 3D cell viability assay (Promega G9681 ...
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bioRxiv - Neuroscience 2022Quote: ... The supernatant was removed and tryptic peptides were eluted from the beads by overnight digestion in 50 µl trypsin solution (V5111, Promega, 0.01 µg/µl in 100 mM ammonium bicarbonate ...
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bioRxiv - Microbiology 2022Quote: Cell viability was measured using a commercially available kit [CellTiter 96 Aqueous One Solution Cell Proliferation Assay (Promega, Madison, USA)] ...
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bioRxiv - Biochemistry 2023Quote: The gel pieces were then incubated with 60 µL of a 50 mM ammonium bicarbonate solution of trypsin (MS grade, Promega) at 4°C for 90 minutes and incubated at 37°C overnight ...
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bioRxiv - Molecular Biology 2024Quote: ... 40 µL of digestion solution (1.25 mM TCEP, 5 mM chloroacetamide, 0.2 µg trypsin/Lys-C mix (Promega, cat#V5073), in 100 mM HEPES pH 8.5 ...
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bioRxiv - Molecular Biology 2024Quote: ... and then a final solution comprised of 17% formamide + 5% Triethalomine + 70% glycerol + 3% Tris (w/v, Tris, Promega, H5135) made in basic DDH20 ...
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Phospholipase C β4 promotes RANKL-dependent osteoclastogenesis by interacting with MKK3 and p38 MAPKbioRxiv - Cell Biology 2024Quote: ... the existing media was removed and replaced with 100 µL of medium containing 20 µL of CellTiter 96 AQueous One solution (Promega). The cells were then incubated for 4 h at 37°C in a humified atmosphere with 5% CO2 ...
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bioRxiv - Microbiology 2022Quote: ... allowing to adhere for 24 h before addition of a serial dilution of inhibitor and measurement of cell viability 72 h later using the CellTiter AQueous One solution (Promega), following manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Cell viability was assessed using a Cell Titer 96 AQueous One Solution Cell Proliferation Assay (Promega Corp, Madison, Wisconsin, USA). Absorbance measurements at 490 nm were normalized to the maximum read per cell line ...
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bioRxiv - Microbiology 2022Quote: ... allowing to adhere for 24 h before addition of a serial dilution of protease inhibitors and measurement of cell viability 72 h later using the CellTiter AQueous One solution (Promega), following manufacturer’s instructions.
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bioRxiv - Developmental Biology 2023Quote: ... After 7 days of culture the MTS cell viability reagent (CellTiter 96® Aqueous One Solution Cell Proliferation Assay, Promega) was added and plates incubated for 4 hours at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... packed column (now loaded with the protein sample) 20 µL of Trypsin digestion solution (1 µg MS-grade Trypsin (Promega) in 50 mM ammonium bicarbonate ...
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bioRxiv - Bioengineering 2023Quote: For luciferase expression 20 uL of undiluted sample was loaded onto a black 96 well-plate then 100uL luciferin solution (Promega) added immediately before reading on a luminometer (Wallac) ...
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bioRxiv - Microbiology 2023Quote: ... Cell proliferation was assessed every 24 h by the MTS assay (CellTiter 96® AQueous One Solution Cell Proliferation, Promega). DMSO-treated cells were used as negative controls and the effects on cell proliferation were expressed as the percentage inhibition compared to the cells of the control group ...
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bioRxiv - Biochemistry 2023Quote: ... cells were starved by 50 μL Hank’s balanced salt solution for 30 min and then incubated in 50 μL CO2-independent media containing 2% GloSensor cAMP Reagent (Promega) for 1 hour ...
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bioRxiv - Immunology 2023Quote: ... The reaction was stopped by KPL TBM Stop Solution (SeraCare, #5150-0021) and plates were read at 450nm on GloMax Explorer Multimode Microplate Reader (Promega).
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bioRxiv - Cancer Biology 2023Quote: ... the plates were allowed to equilibrate to room temperature for 30 minutes and 25 μL of Cytotox Glo solution (Promega) was added to the wells using a Wellmate plate filler ...
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bioRxiv - Systems Biology 2023Quote: Cell viability was measured using a commercial kit (Cell Titre 96 AQueous One Solution cell proliferation assay; Promega, Madison, USA) that uses a tetrazolium salt-based colorimetric assay [20] ...
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bioRxiv - Synthetic Biology 2023Quote: ... 10 μL of stopped reaction samples were mixed with 50 μL of luciferase substrate solution (Luciferase Assay System kit, Promega) in a white flat bottom 96-well microplate ...
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bioRxiv - Microbiology 2023Quote: ... diluted with 40 μL PBS and assayed for secreted gaussia Luciferase (gLuc) activity by injecting 30 μL coelenterazine solution (Promega) followed by 1.6 sec incubation period and a luminescence integration read time of 1.0 sec ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... wells were filled with 20 µl of Bright-Glo reagent per well after discarding the medium and drug solutions (Promega). After 20 minutes of dark incubation ...
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bioRxiv - Cancer Biology 2024Quote: ... the protein precipitate was air-dried and resuspended in 200ul of 25mM ammonium bicarbonate solution and 3ul of trypsin (Promega) and digested overnight at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... and each cell pellet was fully resuspended in 100 µl NanoLuc Assay solution (Promega corp. Madison, WI, USA, cat# 1120). Homogenized solutions were transferred to white flat-bottom 96-well plates (Greiner Bio-One ...
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bioRxiv - Microbiology 2023Quote: ... The columns were then washed three times with a wash solution (90% methanol; 100mM (final concentration) TEAB) before digestion with 100ug trypsin (Promega) (1/10 ...