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Citations for Promega :
1301 - 1350 of 2189 citations for Pops Par Solution Unlabeled 2000 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... 50 µL of ATDC5 Complete Media and 10 µL of CellTiter 96 AQueous One Solution (Promega G3580) were added to each well ...
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bioRxiv - Cell Biology 2023Quote: ... Cell viability was assessed by MTS using CellTiter 96® Aqueous One Solution Cell Proliferation Assay (Promega). For the transwell migration study ...
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bioRxiv - Cell Biology 2023Quote: Cell proliferation was analysed by the CellTiter 96 ® Aqueous One Solution Cell proliferation Assay (Promega, UK). Following cell treatments after incubation period ...
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bioRxiv - Immunology 2023Quote: ... CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS reagent) was obtained from Promega (Cat# G3580). Proteasome inhibitor MG132 (Cat# S2619 ...
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bioRxiv - Plant Biology 2024Quote: ... alkylated (55 mM Iodoacetamide; 1h @ 23°C) and digested in solution with 1:100 trypsin (V5113; Promega). Peptides were acidified to 0.5% (v/v ...
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bioRxiv - Neuroscience 2024Quote: ... at 37°C for 30 minutes prior to the incubation with 2µl RQ1 DNase stop solution (Promega) at 65°C for 10 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... gel regions were diced and proteins were subjected to in-gel digestion with 6 ng/μL trypsin (Promega, Madison, WI, USA) in 50 mM ammonium bicarbonate at 37 °C for 10-12 hours ...
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bioRxiv - Genetics 2022Quote: ... was transiently transfected with the pRL-TK Renilla internal control vector (50 ng) using 4 μL Viafect™ transfection reagent (Promega) into suspension cell lines or adherent cell lines ...
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bioRxiv - Genetics 2019Quote: For strand-specific RT-PCR of the D and U amplicons cDNA was generated from 150 ng of DNase-treated RNA using the GoScript™ Reverse Transcription System (Promega) with either the Downstream-F or Downstream-R primer ...
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bioRxiv - Physiology 2020Quote: ... oligo-dT primed cDNA was synthesized from 500 ng of total RNA using Murine Moloney Leukaemia Virus reverse transcriptase (Promega, USA). qRT-PCR was performed using a ViiA Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Microbiology 2019Quote: ... proteins were firstly digested for 5 h at 37°C with 250 ng rLys-C Mass Spec Grade (Promega, Madison, USA). Samples were then diluted 4-fold with 100 mM Tris HCl pH 8.5 to reach a concentration of 2M urea and then re-incubated overnight at 37°C with 1 µg Sequencing Grade Modified Trypsin (Promega ...
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bioRxiv - Genomics 2019Quote: ... Genotyping PCR was performed on 50 ng genomic DNA using oligos from Table S3 from for 40 cycles using GoTaq (Promega #M3001) (94°C 2min ...
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bioRxiv - Molecular Biology 2019Quote: ... the cells were transfected with 700 ng of plasmid construct (PIB/V5_Gr9) and 3 μl of FuGENE® HD transfection reagent (Promega, USA) in 100 μl of medium per well ...
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bioRxiv - Neuroscience 2020Quote: ... RT-PCR reactions were performed using 20 ng of cDNA of WT2 and NBS8 organoids using GoTaq® DNA Polymerase (Promega). Total RNA from commercially human fetal and adult brain (BioChain® ...
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bioRxiv - Plant Biology 2020Quote: ... using 1 µg of total Arabidopsis or cauliflower mtRNA and 250 ng of a random hexanucleotide mixture (Promega, Cat no. C1181) and incubated for 50 min at 50°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Buffers were removed by two alternating washes of 50 mM TEAB and acetonitrile re-swelled on ice with 12.5 ng/μL trypsin (Promega, catalog # V5111) in 40 μL of 50 mM TEAB ...
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bioRxiv - Biochemistry 2022Quote: ... Total RNA from SVF or 3T3-L1 adipocyte-differentiated cells (at day 10 of differentiation) (200 ng) was reverse transcribed using (MMLV) reverse transcriptase following the manufacturer’s protocol (Promega - Madison, WI). Expression of genes encoding the Pparγ2 ...
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bioRxiv - Physiology 2022Quote: ... In-gel digestion of proteins was performed overnight at 37°C using trypsin (Promega Madison, WI, USA; 40 ng per band), and the resulting peptides were extracted twice (2 x 45 min ...
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bioRxiv - Neuroscience 2022Quote: Magnetic affinity beads were covered with 40 µl of 40 ng/µl sequence grade trypsin in 50mM ammonium bicarbonate pH8 buffer (Promega V5111). The beads were placed in an incubator at 37C overnight ...
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bioRxiv - Microbiology 2021Quote: ... and real-time PCR was performed on 30 ng of DNA with SYBR Green kit GoTaq® qPCR Master Mix (Promega). DNA from non-transduced cell was isolated and amplified at the same time to demonstrate the absence of contamination ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were dried by speedvac before resuspension in 50 mM HEPES (pH 8.0) and the addition of 500 ng Trypsin Gold (Promega, Southampton, UK). Pellets were incubated overnight at 37 °C ...
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bioRxiv - Molecular Biology 2019Quote: ... using 1 μg of total Arabidopsis or cauliflower mtRNA and 250 ng of a random hexanucleotide mixture (Promega, Cat no. C1181) and incubated for 50 min at 50°C ...
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bioRxiv - Genomics 2019Quote: ... sodium bisulfite conversion reagents were used to treat 500 ng of each DNA pool (Methyl EdgeTM Bisulfite Conversion System, Promega, USA). The DMRs for validation were randomly selected from the bioinformatics analysis results ...
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bioRxiv - Cell Biology 2020Quote: Transfections were carried out with 1 μg of total DNA (500 ng for each construct or with empty expression plasmid) and 3 μl of FuGENE ® HD Transfection Reagent (Promega), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... at a concentration of 40 nM or with 1500 ng of pcDNA3.1-DR5-AS with the Fugene HD transfection reagent (Promega, United States) in a 2-mL final volume ...
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bioRxiv - Molecular Biology 2022Quote: ... 250 ng of the pGL3-promoter vector (or the constructions derived from it) and 125 ng of the pCMV-β-gal plasmid (Promega) were used for transfection ...
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bioRxiv - Neuroscience 2022Quote: ... The gel pieces were dried with 200 μl of ACN and digested with 10 ng/μl Lys-C (sequencing grade, Promega, USA) in 50 mM NH4HCO3 overnight at 37 °C (14).
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bioRxiv - Molecular Biology 2024Quote: ... The antiHER2 protein was produced from CD81-antiHER2-tGFP vector (600 ng) using TNT® Quick Coupled Transcription/Translation Systems (Promega), following manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... 25 ng of c-DNA was used per reaction in each well containing the 2X SYBR green PCR master mix (Promega, USA) along with appropriate primers ...
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bioRxiv - Immunology 2023Quote: A total of 125 ng genomic RVFV clone 13 RNA was either mock treated or incubated with 40 U CIAP (Promega, M1821), 2 U RNaseIII (Ambion ...
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bioRxiv - Immunology 2023Quote: ... 25 ng of c-DNA was used per reaction in each well containing the 2X SYBR green PCR master mix (Promega, USA) along with appropriate primers ...
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bioRxiv - Microbiology 2023Quote: ... Excess IAM was quenched by increasing the DTT concentration to 25 mM (1500 rpm, 15 min) and the digest was started by the addition of 500 ng trypsin (Promega, V5111) per sample ...
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bioRxiv - Plant Biology 2022Quote: ... First-strand cDNA was synthesised using 1 µg RNA and 500 ng µl-1 Oligo(dT)15 primer (Promega, Southampton, UK), denatured at 65°C for 5 min ...
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bioRxiv - Plant Biology 2023Quote: ... a WGBS library was constructed using 500 ng of purified genomic DNA spiked with 0.1% (w/w) unmethylated Lambda DNA (Promega, Madison, WI). Briefly ...
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bioRxiv - Immunology 2023Quote: ... Pre-digestion of bound proteins was performed by addition of 200 ng / µl LysC (FUJIFILM Wako Pure Chemical Corporation, 125-02543) and trypsin (Trypsin Gold, Mass Spec Grade, Promega, V5280) mixed 1:1 ...
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bioRxiv - Systems Biology 2024Quote: ... protein digestion was performed overnight by addition of 50 nL of 0.01 ng nL-1 Lys-C (MS grade, Promega, Madison, USA) and 50 nL of 0.04 ng nL-1 trypsin (Promega ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were digested at 47 °C for two hours with 600 ng of mass spectrometry grade Trypsin/LysC mix (Promega, V5113). The digested peptides were then eluted with two 35 µl increments of 0.2% formic acid in water and two more 40uL increments of 80% acetonitrile with 0.2% formic acid in water ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were digested at 47 °C for two hours with 600 ng of mass spectrometry grade Trypsin/LysC mix (Promega, V5113). The digested peptides were then eluted with two 35 µl increments of 0.2% formic acid in water and two more 40uL increments of 80% acetonitrile with 0.2% formic acid in water ...
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bioRxiv - Genetics 2024Quote: ... 13ng of pCMV-VP16 (EV) or pCMV-LXRα-VP16 and 13 ng of GAL4-Co-factor vector and 13ng of pRL-TK (Promega, 2241) using Lipofectamine 3000 ...
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bioRxiv - Biochemistry 2024Quote: HEK293T cells seeded in 12-wells plates coated with poly-D-lysine were transfected with 500 ng of empty vector pHTN HaloTag CMV-neo (Promega G7721) or pCEFL-HaloTag-P-Rex1 constructs and co-transfected with 500 ng of SRE-firefly luciferase and 50 ng of Renilla luciferase plasmids ...
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bioRxiv - Plant Biology 2023Quote: ... LUC activity was measured with 20 mL of protein sample and 100 mL of LUC assay reagent (Promega). Promoter activation was calculated as (rate of MUG accumulation / LUC luminescence ...
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bioRxiv - Molecular Biology 2021Quote: ... 40U/ml RNasin Plus RNase Inhibitor (Promega), DNase I ...
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bioRxiv - Molecular Biology 2021Quote: ... 40U/ml RNasin Plus RNase Inhibitor (Promega), 0.015% digitonin ...
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bioRxiv - Molecular Biology 2021Quote: ... 40U/ml Rnasin Plus Rnase Inhibitor (Promega), Dnase I ...
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bioRxiv - Molecular Biology 2022Quote: ... and 110 U/ml of RNasin (Promega)) rolling for 30 min at 5°C ...
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bioRxiv - Molecular Biology 2021Quote: ... 40 U/mL RNasin plus (Promega, N2615), 20 U/mL SuperaseIn RNase Inhibitor (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... a 20 mg/mL Proteinase K (Promega) solution for 1 h at 37°C and a 10x SYBR Green I (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: ... 500 unit/ml RNasin Plus (Promega, N2615), 2 mM DTT ...
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bioRxiv - Cell Biology 2022Quote: ... 2 mg/ml proteinase K (Promega, v3021)) at 37 °C overnight followed by addition of 10 μl of 3M potassium acetate and incubation at room temperature for 1h ...
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bioRxiv - Microbiology 2021Quote: ... and also 0.3 mg/ml G418 (Promega) was added to the culture medium at every other passage ...