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Citations for Promega :
1351 - 1400 of 5339 citations for ssc mir 411 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... qRT-PCRs were performed using GoTaq qPCR SYBR Green chemistry (Promega) and a Bio-Rad CFX 96 Touch System (Bio-Rad) ...
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bioRxiv - Cell Biology 2023Quote: ... with HaloTag amplified by PCR from pHTN-HaloTag CMV-neo (Promega) and mEmerald amplified by PCR from mEmerald-Sec61b-C1 (Addgene #90992) ...
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bioRxiv - Microbiology 2023Quote: ... This was followed by Q-PCR using Sybr green mix (Promega). 40 cycles were performed and relative expression of Rv0282 was determined in RS ...
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bioRxiv - Genetics 2024Quote: ... and 10 µL of power SYBR green PCR master Mix (Promega). The real-time qPCR was performed using a fluorescent quantitative detection system (FQD-96A ...
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bioRxiv - Developmental Biology 2024Quote: ... After purification (Promega Wizard SV Gel and PCR clean-up system), the concentration of the HRT was adjusted to 100 ng/µl as measured with Nanodrop (Thermo Scientific) ...
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bioRxiv - Developmental Biology 2024Quote: ... After purification (Promega Wizard SV Gel and PCR clean-up system), the concentration of the HRT was adjusted to 100 ng/µl as measured with Nanodrop (Thermo Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... 4,7 μL of PCR amplification-grade water (Promega Corporation, Wisconsin, USA) and 2 μL of sample DNA (about 20 ng) ...
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bioRxiv - Genomics 2020Quote: ... At the time of analysis samples were diluted with an equal volume of LDH Detection Reagent and equilibrated for 60 minutes at room temperature before reading the luminescence (GloMax 96 Microplate Luminometer, Promega Corp, Madison, WI) in relative luminescence units (RLU) ...
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bioRxiv - Molecular Biology 2022Quote: ... Luciferase activity was analyzed using the Dual-Luciferase® Reporter Assay System and read with GloMax®-Multi Detection System (Promega, Madison, WI).
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bioRxiv - Plant Biology 2020Quote: ... PCR products amplified by gene-specific RT-qPCR primers listed in Supplemental Table S1 were cloned in pGEM-T Easy vector (Promega, Madison, Wisconsin, USA) prior to their sequencing ...
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Evolution of sex hormone binding globulins reveals early gene duplication at the root of vertebratesbioRxiv - Evolutionary Biology 2020Quote: ... Reverse transcription (RT) was performed using 1 μg of RNA for each sample with M-MLV reverse transcriptase and random hexamers (Promega, Madison, WI, USA). Briefly ...
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bioRxiv - Neuroscience 2021Quote: ... plates were removed from the incubator after 70 hours and 10 µL of 4x RT-Glo substrate and enzyme per well (Promega, Madison, WI, USA) diluted in P/S free medium ...
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bioRxiv - Microbiology 2020Quote: ... followed by a reverse transcription using a universal degenerated oligo specific to all 5′ non-coding sequences of BTV-1 segments (BTV/Uni1; 5′ GTTAAAWHDB 3′) and the GoScript Reverse Transcription (RT) System (Promega, Madison, WI, USA). The cDNAs obtained for the segment 7 were then quantified with a real time quantitative PCR (qPCR) ...
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bioRxiv - Plant Biology 2022Quote: ... benthamiana leaf discs were ground to a fine powder in liquid nitrogen in 2 ml Eppendorf Safe-Lock tube (1 leaf disc in each tube) and subsequently incubated 5 min at room temperature (RT) with 200 µl of the Passive lysis buffer (Promega catalog number E1910). The resulting crude leaf extract was centrifuged at 20,000 g for 2 min at RT ...
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bioRxiv - Biochemistry 2024Quote: ... was synthesised from 2 µg of total RNA using the Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT; Promega, Madison, WI, USA) using random primers and oligo-dT primers (3:1 mol ...
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bioRxiv - Molecular Biology 2023Quote: Kit (Promega). For transposition with an additional plasmid pCas12k ...
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bioRxiv - Genomics 2021Quote: ... thawed samples were diluted with an equal volume of LDH Detection Reagent and equilibrated for 60 minutes at room temperature before reading the luminescence (GloMax 96 Microplate Luminometer, Promega Corp Madison, WI USA) in relative luminescence units (RLU) ...
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bioRxiv - Microbiology 2021Quote: ... cDNA was synthesized using 5 μl of the eluent as template using the U3 antisense primer 5’ TGTGTGGTAGATCCACATCG-3’ and M-MLV RT RNase [H-] (Promega, WI. Cat. No. MR3681) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... Reverse transcriptase PCR was performed using M-MLV reverse transcriptase (Promega, USA). qRT-PCR was performed using SYBR® Premix Ex Taq™ (Takara ...
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bioRxiv - Molecular Biology 2020Quote: ... Genomic PCR was performed using GoTaq® G2 DNA Polymerase (Promega, M7841) from 10ng gDNA (Forward ...
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bioRxiv - Molecular Biology 2021Quote: ... Following gel extraction using Wizard SV Gel and PCR CleanUp System (Promega), 500 ng of purified and linearized vector and 200 ng of each specific gene were treated with T4 DNA polymerase enzyme (Fermentas ...
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bioRxiv - Molecular Biology 2021Quote: ... The PCR fragment was cloned into the pGEM-T Easy vector (Promega), whose polycloning site is flanked by two phage promoters (T7 and SP6 ...
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bioRxiv - Biochemistry 2019Quote: ... PCR products for the peptide genes were subcloned into pGEM-T (Promega) and sequenced ...
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bioRxiv - Evolutionary Biology 2020Quote: ... PCR products were cloned into the pGEM®-T Easy Vector (Promega), and three clones per individual were picked ...
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bioRxiv - Neuroscience 2019Quote: ... The PCR product was subcloned into pGEM-T Easy vector (A1360, Promega). An EcoRI-digested fragment containing TET1CD was then ligated to EcoRI-digested pCAGGS vector (Niwa et al ...
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bioRxiv - Developmental Biology 2020Quote: ... All PCR products were TOPO cloned into pGEM-T Easy vectors (Promega) and sequence verified by Sanger sequencing ...
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bioRxiv - Neuroscience 2019Quote: ... The purified PCR product was inserted into the pGEMT easy vector (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... Plants were genotyped by PCR using the GoTaq® polymerase (Promega®) and the indicated primers (Table S8) ...
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bioRxiv - Plant Biology 2020Quote: ... The PCR product was purified and DNA was digested by ApaI (Promega), then the digest was purified ...
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bioRxiv - Physiology 2020Quote: ... the PCR products were cloned into the pGEM-T Easy vector (Promega Biosciences ...
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bioRxiv - Microbiology 2019Quote: ... Transformants were confirmed by colony PCR using Go Taq G2 polymerase (Promega) and primers p181 (5’-GCGAAGATAACAGTGACTCTA-3’ and p54 (5’-CGGCTCTGATTAAATTCTGAAG-3’ ...
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bioRxiv - Developmental Biology 2021Quote: ... PCR products were TA-cloned into the pGEM-T Easy vector (Promega) or PCRII vector (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... PCR amplifications were performed with GoTaq G2 DNA polymerase (Promega, Madison, USA) using a peqSTAR 2X PCR machine from PEQLAB ...
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bioRxiv - Plant Biology 2022Quote: ... PCR was performed using GoTaq Green Mastermix (Promega Corp., Madison, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... PCR followed standard conditions using GoTaq®Green Master Mix (Promega corp.), Ta= 58°C ...
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bioRxiv - Immunology 2022Quote: ... PCR products were ligated into pGEM-T easy vectors (Promega, Walldorf, Germany) and transformed into JM109 E ...
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bioRxiv - Cell Biology 2022Quote: ... Genomic PCR was carried out using GoTaq HotStart Green Master Mix (Promega), as per manufacturer’s instruction (TRAIP F ...
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bioRxiv - Microbiology 2022Quote: ... The PCR product was cloned in a pGEM-T easy vector (Promega), digested with BamHI and NotI ...
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bioRxiv - Microbiology 2022Quote: ... The PCR product was cloned in a pGEM-T easy vector (Promega), digested with BglII and XhoI ...
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bioRxiv - Neuroscience 2022Quote: PCR mix was run with GoTaq Green Master Mix 2x (Promega, M712B). The PCR amplification process was as follows ...
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bioRxiv - Neuroscience 2022Quote: ... RNA (1 μg) from each sample was reverse transcribed for PCR (Promega). PCR was conducted using GoTaq DNA polymerase (Promega) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Standard PCR reactions were performed using Go Taq Green Master Mix (Promega) and 0.5μmol L−1 of the primer ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Purified PCR product was ligated in the pGEM-T Easy vector (Promega) and transformed into chemically competent JM109 cells (Promega) ...
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bioRxiv - Genetics 2021Quote: ... All other polymerase chain reactions (PCR) were performed using the GoTaq (Promega) enzyme system as per manufacturer instructions (95°C for 2 min ...
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bioRxiv - Plant Biology 2020Quote: ... cRT-PCR products were cloned into pGEM®-T Easy vector (Promega) and more than 10 clones/cRT-PCR product were sequenced.
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bioRxiv - Biochemistry 2020Quote: TNT T7 Quick for PCR DNA was from Promega (Madison, WI, USA). Dog pancreas ER rough microsomes were from tRNA Probes (College Station ...
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bioRxiv - Neuroscience 2021Quote: ... Separate PCR reactions were prepared using the GoTaq® Master Mix (Promega) and primers targeting Abi3 exon 5-7 (5’→3’ Fw ...
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bioRxiv - Cell Biology 2021Quote: ... amplified PCR products (8 μl) were further digested by ApaI (Promega #R6361) for the m.3243A>G and HapII (Promega #R6311 ...
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bioRxiv - Cell Biology 2020Quote: ... The PCR product was cloned into the pGEM-T easy vector (Promega) and sequences were verified ...
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bioRxiv - Biochemistry 2021Quote: TNT T7 Quick for PCR DNA was from Promega (Madison, WI, USA). Dog pancreas ER column washed rough microsomes were from tRNA Probes (College Station ...