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Citations for Promega :
1201 - 1250 of 5641 citations for ssc mir 411 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... For HiBiT detection, 25µL of HiBiT lytic reagent (1:25 substrate, 1:50 LgBiT) was added to the lysate (Promega, cat# N3050). For FFluc detection ...
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bioRxiv - Synthetic Biology 2020Quote: ... 5 μL normalised extract were diluted to 30 μL in passive lysis buffer and mixed with 30 μL Nano-Glo® HiBiT Extracellular Detection Reagent (Promega) and luminescence was detected as above ...
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bioRxiv - Synthetic Biology 2021Quote: The effect of DMSO and methyl ester version compounds (compound 34–38) on cell viability was determined using a CellTiter-GloTM ATP detection system (Promega #7573). For testing DMSO tolerance ...
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bioRxiv - Genetics 2021Quote: ... The luciferin is then utilized in a firefly luciferase reaction to generate a bright luminescent signal determined as RLUs using a GloMax-Multi Detection System: Luminometer (Promega, USA). 2/ Colorimetric detection of senescence-associated β galactosidase following the manufacturer’s protocol (Cell Signaling #9860) ...
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bioRxiv - Cell Biology 2020Quote: ... The assay plates were then equilibrated to room temperature for 15 minutes before the addition of 25 µl of Nano-Glo HiBiT Lytic Detection System (Promega #N3030.) The plates were then shaken at 300 rpm for 2 minutes on an orbital shaker ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell viability was quantified by detection of ATP content in the culture medium with CellTiter-Glo® Luminescent Cell Viability Assay (Promega) by following the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Assessment of total cellular HiBiT-GCase protein abundance was performed using the Nano-Glo® HiBiT Lytic Detection System (Cat.#N3040, Promega), according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2023Quote: ... Crude extract (10 μl) was mixed with 40 μl of LARII and FLuc activity was determined using a GloMax®-Multi Detection System (Promega) with a 2 s delay and a 10 s integration times ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... opaque 96-well plates and reagents were added according to the manufacturer’s protocols for the Nano-Glo® HiBiT Extracellular Detection System (Promega Corp.). After a 10-minute incubation step ...
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bioRxiv - Cell Biology 2023Quote: ... incubated 3 h at 37°C and alkaline phosphatase (SEAP) activity determined measuring the absorbance at 600 nm using GloMax®-Multi Detection System (Promega).
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bioRxiv - Microbiology 2023Quote: ... The amount of pseudoviruses prepared was quantified by the HiBiT assay using Nano Glo HiBiT lytic detection system (Promega,Cat# N3040) as previously described.1–5 ...
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bioRxiv - Bioengineering 2023Quote: ... 10 µl supernatant was incubated with 10 µl of freshly prepared nucleotide detection reagent for UDP-Glo assays (Promega, Cat#: V6961) according to manufacturer’s specifications ...
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bioRxiv - Microbiology 2023Quote: ... The detection of intracellular ATP level was conducted by BacTiter-Glo™ Microbial Cell Viability Assay (Promega Corporation, Leiden, the Netherlands). ATP disodium salt (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... PCR positive clones were then expanded and tested for their response to pomalidomide treatment (positive control compound) by Nano-Glo® HiBiT Lytic Detection assay (Promega). The PCR products of 4 positive clones that responded to pomalidomide treatment were subjected to next generation sequencing to confirm the insertion of the HiBiT tag at the appropriate position of the HBG1 gene.
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bioRxiv - Molecular Biology 2020Quote: First strand synthesis was performed in 25 μl reaction volumes using 5 μl of MMLV-RT 5X buffer (Promega, Wisconsin, UK), 1.25 μl of dNTPs (10mM ...
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bioRxiv - Microbiology 2021Quote: ... was added to each well and incubated for 1 h at RT before fluorescence intensity was read on a Glomax® Explorer Fully Loaded (Promega) with emission wavelengths of 500-550 nm and an excitation wavelength of 475 nm and graphs were generated using GraphPad Prism (V.8.4.1) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Real-Time PCR Diagnostic Panel17 and a protocol for quantifying the SARS-CoV-2 subgenomic E gene RNA (E SgRNA)18 using the GoTaq® Probe 1-Step RT-qPCR System (Promega). For quantification of SARS-CoV-2 using the nCoV assay ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Real-Time PCR Diagnostic Panel17 and a protocol for quantifying the SARS-CoV-2 subgenomic E gene RNA (E SgRNA)18 using the GoTaq® Probe 1-Step RT-qPCR System (Promega). For quantification of SARS-CoV-2 using the nCoV assay ...
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bioRxiv - Plant Biology 2021Quote: ... RT-qPCR assays were carried out in triplicate using SYBR® Green chemistry with GoTaq® qPCR Master Mix (Promega, UK) on a StepOne™ Real-Time PCR Machine (Applied Biosystems ...
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bioRxiv - Microbiology 2022Quote: ... then diluted 1:5 with nuclease-free water before 2 µL was used as template in a 10 µL GoTaq 1-Step RT-qPCR (Promega, A6020) reaction (69) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Amplification reactions were run in a 20 μL volume using the GoTaq 1-Step RT-qPCR System (Promega, Madison WI, USA) and the recommended manufacturer’s instructions as described in Cervera et al ...
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bioRxiv - Microbiology 2020Quote: ... samples were heated at 80 °C for 15 min and cooled to RT before addition of 1 μg of trypsin (Promega, V511A). Samples were digested at 37 °C overnight ...
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bioRxiv - Microbiology 2023Quote: The viral RNA derived from the lung and nasal turbinate samples was quantified using a protocol for quantifying the SARS-CoV-2 sub-genomic E gene RNA (sgE)29 using the GoTaq® Probe 1-Step RT-qPCR System (Promega).
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Throat colour polymorphism in relation to sex and body size of the Litter skink, Lankascincus fallaxbioRxiv - Zoology 2022Quote: ... The PCR amplification was carried out in 25 μl volumes employing 35 cycles with an annealing temperature of 55 °C following standard PCR protocols with Promega PCR master mix (Promega Corporation, Madison, Wisconsin, USA). The success of the PCR amplification of each sample was confirmed through Gel electrophoresis using 5 μl of each sample ...
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bioRxiv - Molecular Biology 2023Quote: ... Japan) after PCR amplification and extrication from the agarose gel using Wizard® SV Gel and PCR Clean-Up System (Promega, Madison, WI, USA). The threshold was set to 0.6 ΔRn ...
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bioRxiv - Neuroscience 2022Quote: ... PCR products were ligated into pGEMT.easy (Promega, Madison, WI, US) and sequenced with Sanger sequencing ...
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bioRxiv - Molecular Biology 2020Quote: PCR reactions were carried out using Go-Taq enzyme (Promega) with 1 μl of cDNA diluted 1:5 ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR was performed using GoTaq G2 DNA Polymerase (Promega, USA) according to manufacturer’s instructions.
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bioRxiv - Microbiology 2019Quote: ... PCR products were cloned into pGEM-T Easy vector (Promega) and sequenced using T7 promoter forward primer by Sanger sequencing ...
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bioRxiv - Genetics 2019Quote: ... The PCR products were cloned into pGEM-T easy (Promega) and the sequence of the resulting plasmids was verified ...
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bioRxiv - Developmental Biology 2019Quote: ... PCR products cloning into pGEM-T-easy vector system (Promega). We Sanger sequenced 10 colonies per sample using M13 primers using the above described method and results were analysed with BISMA online tool (Bisulfite Sequencing DNA Methylation Analysis - http://services.ibc.uni-stuttgart.de/BDPC/BISMA/ ...
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bioRxiv - Genetics 2020Quote: ... The PCR is carried using GoTaq DNA polymerase (Promega, UK) and the following PCR conditions ...
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bioRxiv - Developmental Biology 2021Quote: ... PCRs were conducted using the GoTaq Master Mix (Promega Corporation), and products were run on a 1% agarose gel in 1X TAE Buffer.
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bioRxiv - Neuroscience 2021Quote: ... and PCR was performed using GoTaq green master mix (Promega). Primers used were ...
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bioRxiv - Plant Biology 2020Quote: ... or Wizard SV Gel and PCR Clean-Up System (Promega), and then sequenced.
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bioRxiv - Microbiology 2021Quote: ... a PCR fragment was amplified using GoTaq DNA polymerase (Promega) and subcloned in a TOPO cloning vector (Invitrogen) ...
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bioRxiv - Cancer Biology 2020Quote: ... PCR amplification was performed using GoTaq Green Master Mix (Promega) or Phusion High-Fidelity DNA polymerase (NEB ...
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bioRxiv - Synthetic Biology 2022Quote: ... PCR products were purified prior to quantification via QuantiFluor (Promega) and combined into an equimolar pool ...
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bioRxiv - Neuroscience 2020Quote: ... PCR products were cloned by A-tailing into pGEMTeasy (Promega) and sequenced ...
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bioRxiv - Plant Biology 2021Quote: ... PCR was conducted using 1x Green GoTaq Flexi Buffer (Promega), 2.5 mM MgCl2 ...
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bioRxiv - Immunology 2021Quote: ... using a one-step PCR mix (Promega, catalog no. A6120). Zika virus was detected using primer pair ...
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bioRxiv - Neuroscience 2020Quote: ... qRT-PCR was performed using GoTaq qPCR Master Mix (Promega) according to the recommended protocol ...
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bioRxiv - Microbiology 2021Quote: ... Colony PCR was performed using GoTaq Green Master Mix (Promega) in 50 µL reactions (1 µL of cell suspension ...
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bioRxiv - Developmental Biology 2021Quote: ... PCR amplifications were carried out using GoTaq G2 polymerase (Promega) at 1.5 mM MgCl2 using the following cycling parameters ...
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bioRxiv - Microbiology 2021Quote: ... The PCR products were cloned into the pGEMT vector (Promega,) and recombinant plasmids were transformed in E ...
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bioRxiv - Biochemistry 2020Quote: ... The PCR product was cloned into pGEM®-T (Promega) and sequenced.
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bioRxiv - Microbiology 2021Quote: Confirmation PCRs were performed using GoTaq Green master mix (Promega), per the manufacturer’s instructions ...
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bioRxiv - Genetics 2019Quote: ... Purified PCR products were TA-cloned into pGEM-T (Promega), and sequenced at the McGill/Genome Quebec Innovation Centre ...
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bioRxiv - Microbiology 2019Quote: ... The PCR products were cloned into pGEM-T-Easy (Promega) and multiple clones were Sanger-sequenced.
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bioRxiv - Molecular Biology 2020Quote: ... the PCR fragments were cloned into the psiCHECK2 vector (Promega) downstream of Renilla luciferase ...