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Citations for Promega :
1151 - 1200 of 5641 citations for ssc mir 411 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... PCR amplifications were undertaken using GoTaq DNA polymerase (Promega) and PCR cycles of 95 °C for 5 min ...
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bioRxiv - Microbiology 2022Quote: ... Genotyping PCRs were performed using GoTaq Green Mastermix (Promega) (Fig ...
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bioRxiv - Plant Biology 2024Quote: ... Genotyping PCRs were conducted using GoTaq DNA Polymerase (Promega) in accordance with the manufacturer’s guidelines ...
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bioRxiv - Biochemistry 2024Quote: ... the PCR products were ligated (T4 DNA ligase, Promega) into similarly restricted pYES2 ...
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Hepatitis B Virus genomes associate with cellular sites of DNA damage by inducing replication stressbioRxiv - Microbiology 2024Quote: ... transferred to a PCR clean-up column (Promega, A9282) and centrifuged ...
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bioRxiv - Cell Biology 2023Quote: ... the PCR product was cloned into pGEMTeasy (Promega A1380). The mutagenesis of the EPIYA sites were done one after the other using the kit Quick change II site-directed mutagenesis (Aligent #200523) ...
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bioRxiv - Cell Biology 2023Quote: ... the PCR product was cloned into pGEMTeasy (Promega A1380). The cagA fragment was digested with KpnI and EagI and subcloned into the lentivirus entry vector pEF-1α/pENTR (Addgene ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were cloned into vector pGEM-T (Promega). CRISPR A and B start naturally with a 6 nt cassette consisting of adenines and guanines that we used for synchronisation of transcription (see below) ...
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bioRxiv - Microbiology 2023Quote: ... unlabeled PCR was cloned into pGEM-T Easy (Promega) and positive clones were checked by amplification with the vector-based primers M13f and M13R.
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bioRxiv - Developmental Biology 2023Quote: ... PCR products were subcloned in pGEM-T-easy (Promega) and sequenced ...
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bioRxiv - Physiology 2023Quote: ... The PCR products were subcloned to T-vector (Promega) and sequenced ...
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bioRxiv - Cell Biology 2022Quote: ... After PCR clean-up (Promega #A9281, Madison, WI, USA), A-tailing of the PCR products with Taq Polymerase was performed as ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR was performed using GoTaq Flexi DNA Polymerase (Promega), and PCR products were migrated on agarose gels ...
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bioRxiv - Bioengineering 2023Quote: PCR was performed using GoTaq Flexi DNA polymerase (Promega) to amplify both junctions using primers listed in Table 1 and routinely 500 ng of genomic DNA from parental cells or each recombinant clone as template in 25 μl reactions ...
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bioRxiv - Microbiology 2023Quote: ... Each PCR reaction contained 0.25 µl Taq polymerase (Promega), 2.5 µl 10 µM forward and reverse primers ...
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bioRxiv - Microbiology 2023Quote: ... Each PCR reaction contained 0.25 µl Taq Polymerase (Promega), 2.5 µl 10 uM forward and reverse primers ...
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bioRxiv - Neuroscience 2024Quote: ... Ligated PCR products inserted into the pNL1.1 vector (Promega) by KpnI and EcoRV enzyme sites ...
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bioRxiv - Immunology 2021Quote: ... RT-qPCR to detect ZIKV was performed in a 20 μL reaction final volume containing GoTaq one-step RT-qPCR master mix (Promega), 25 ng of sample RNA ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were subjected to digestion of Lys-C (Wako Chemicals) (1:50) for 2 h at RT and then to trypsin (Promega) (1:50 ...
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The AAA-ATPase ATAD1 and its partners promote degradation of desmin intermediate filaments in musclebioRxiv - Cell Biology 2022Quote: ... modified with 10mM Iodoacetamide in 100 mM ABC (at the dark, RT, 30 mins) and digested in 10% Acetonitrile and 10 mM ABC with modified trypsin (Promega) at a 1:10 enzyme-tosubstrate ratio overnight at 37°C ...
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bioRxiv - Molecular Biology 2019Quote: ... 5 μl of Reverse Transcription Mix (10 μl of 10 mM dNTPs and 40 μl of 5X MMLV RT Buffer Promega), 1 μl of 10 mM ddTTP for the sequencing reaction and 1 μl of MMLV Reverse Transcriptase RNAse H minus (Promega ...
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bioRxiv - Synthetic Biology 2019Quote: ... Backbone and inserts were ligated either for 3 h at RT or overnight at 4°C using T4 ligase (Promega). All vector sequences (Table S5 ...
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bioRxiv - Plant Biology 2020Quote: ... The concentrations of the resulting cDNAs were adjusted to 10 ng/µL and 2 µL were used for RT-qPCR reactions performed with the GoTaq qPCR Master Mix (Promega) in a final volume of 10 µL ...
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bioRxiv - Molecular Biology 2020Quote: ... Resulting ribosomal complexes were analyzed by toe-printing as described (Pisarev et al., 2007) using avian myeloblastosis virus reverse transcriptase (AMV RT) (Promega) and [32P]-labeled oligonucleotide primers complementary to HalV nt ...
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bioRxiv - Immunology 2022Quote: ... Cells were then incubated at 37°C for 72 h after which they were lysed for 20 min on a shaker platform at RT by addition of Reporter Lysis Buffer (Promega). Luciferase signal was determined from cell lysates by adding the Bright-Glo Luciferase buffer (Promega ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 μL of the annealed oligos were ligated with 0.04 ng digested PspCAs13b crRNA backbone in 10 μL of T4 ligation buffer (3 h, RT) (Promega, M1801). All PspCas13b crRNA spacer sequences used in this study are listed in Supplementary File 1 ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were washed twice with UA buffer and twice with 50mM ammonium bicarbonate prior to digest of the immobilized proteins on the filter for 2 h at RT using 1 mg Lys-C (Wako Chemicals) and for 16 h at 37C using 2 mg trypsin (Promega). Tryptic peptides were collected by centrifugation (10 min at 14,000 g) ...
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bioRxiv - Microbiology 2020Quote: ... The reactions were subjected to primer extension at 42°C for 45 min using 1 unit of MMLV-RT (Promega) and 0.5mM of dNTPs ...
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bioRxiv - Developmental Biology 2019Quote: ... cells into micro-tubes containing 5 µl of cold 1X SuperScript IV VILO Master Mix for two-step RT-qPCR containing 6 units RNasin (Promega) and 0.5% (v/v ...
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bioRxiv - Microbiology 2022Quote: ... Complementary DNA was synthetized from 500 ng total RNA by using random hexamers and moloney murine leukemia virus (M-MLV) reverse transcriptase (RT) Point Mutant Synthesis System (Promega). Quantification was performed by using the LightCycler 480 Universal Probe Library System (Roche) ...
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bioRxiv - Cancer Biology 2024Quote: ... 1ug of each RNA sample was reverse-transcribed using Moloney Murine Leukemia Virus reverse transcriptase (M-MLV-RT, Promega, #M1701) and random hexamer primers (Thermo Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... 1 µg of RNA was then used to synthesize cDNA in a 20 µl reverse transcription reaction using 5X First Strand Buffer and MMLV-RT (Promega) with random hexamers ...
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bioRxiv - Microbiology 2023Quote: ... The reactions were subjected to primer extension at 42°C for 45 min using 1 unit of MMLV-RT (Promega) and 0.5 mM of dNTPs ...
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bioRxiv - Immunology 2022Quote: ... followed by retrotranscription to cDNA with M-MLV RT, RNase H(–) point mutant (Promegain presence of buffer, nucleotides and RNasin Plus RNase Inhibitor (Promega). Reaction was then incubated at 40 °C for 10 min ...
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bioRxiv - Microbiology 2022Quote: ... and alkylated with 5 mM of iodoacetamide 30 min in the dark at RT°C prior to digestion with 5 μg of trypsin Gold (Promega) for 15 h at 37 °C ...
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bioRxiv - Bioengineering 2023Quote: ... The day after the plates were pre-cooled (RT, 15’) supplemented with luciferase substrate (Bright Glo Luciferase Assay System, Promega) incubated (RT ...
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bioRxiv - Biochemistry 2022Quote: ... RNA was isolated 24 hours after transfection and the pattern of alternative splicing was assessed by RT (with oligo-d(T) / random hexamer) and AMV Reverse Transcriptase (Promega) and PCR with GoTaq (Promega ...
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bioRxiv - Microbiology 2023Quote: ... and then reverse transcribed into complementary DNA (cDNA) using 30 enzyme units(U) of the MMLV-RT (Promega, WI, USA) and 1μM of the labeled primer ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were digested with 2 μg of lysyl endopeptidase (Wako) at RT for overnight and were further digested overnight with 2 μg trypsin (Promega) at RT ...
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bioRxiv - Neuroscience 2023Quote: ... Blots were then incubated with blotting buffer containing 1:200 LgBiT for 1-2 h with rocking at RT (N2410, Promega). Next ...
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bioRxiv - Immunology 2023Quote: ... cDNA was generated from 20-100 ng of RNA using the GoTaq 2-step RT-qPCR System (Promega, cat# A6110). qPCR was performed with SYBR Green on a StepOnePlus real-time PCR system (Applied Biosystems) ...
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bioRxiv - Neuroscience 2024Quote: Nuclear fractions from Con (n=13) and DLB (n=12) were pretreated with DNAse (50 u/ml, 15 mins RT, RQ1 DNAse, Promega), were adjusted to 80 ng /µl with LDS sample buffer (Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... proteins were alkylated in 5 mM iodoacetamide for 1 hour in the darkness at RT and digested overnight at 37°C with 1 µg trypsin (Trypsin Gold Mass Spectrometry Grade, Promega). Peptides were recovered by centrifugation ...
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bioRxiv - Cell Biology 2020Quote: ... Then samples were diluted 3 times in the buffer and 1 µg trypsin (Gold. Promega) was added overnight at 30°C ...
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bioRxiv - Cell Biology 2020Quote: ... followed by a 4 times dilution in Tris buffer and an overnight trypsin digestion (Promega) at a ratio 1/100 ...
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bioRxiv - Immunology 2023Quote: ... Luminescence was recorded with a 0.3 second integration time on a GloMax luminometer system (Promega).
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bioRxiv - Immunology 2023Quote: ... Luminescence was recorded with a 0.3 second integration time on a GloMax luminometer system (Promega).
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bioRxiv - Cancer Biology 2023Quote: ... In this time the Fugene HD mix (8.6 μl Fugene HD (Promega, Madison, WI, USA) in 110 μl Opti-MEM was prepared and mixed thoroughly ...
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bioRxiv - Microbiology 2022Quote: ... then dipped in a well of a 96-well PCR plate filled with 20 µl of a gyrB PCR mix (4 µl of 5x GoTaq buffer (Promega, Madison, WI, USA), 0.4 µl of 10mM dNTP ...
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bioRxiv - Molecular Biology 2020Quote: ... The expression of extracellular HiBiT-BNLN was normalized to the total intracellular pool of HiBiT-BNLN by lysing the cells and measuring luminescence with Nano-Glo HiBiT lytic detection system (Promega, N2422), following the protocol.