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Citations for Promega :
1201 - 1250 of 4570 citations for Human Immunodeficiency Virus Tat Protein HIV 1 Clade C TV019 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2023Quote: ... washing, and digestion with lysyl endopeptidase (Lys-C, Wako) and sequencing-grade trypsin (Promega). The capture and digestion steps were performed with a Versette liquid handling robot (ThermoFisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: N-terminal large (LgBit) and C-terminal small (SmBit) fragments derived from NanoLuc (Promega) were fused to the C-terminus of B56δ and inserted downstream of its N-arm at residue 103 ...
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bioRxiv - Cancer Biology 2023Quote: ... Libraries were incubated at 25°C for 5 days before CellTiter-Glo assays (Promega) were performed ...
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bioRxiv - Biochemistry 2023Quote: Tryptic digestion was performed at 37° C with 125 μL Trypsin (Promega Cat# V511X) in digestion buffer (100 mM TEAB pH 8.5 ...
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bioRxiv - Molecular Biology 2023Quote: ... synthesized from Gene Universal (USA) at 4°C overnight (T4 DNA ligase, Promega, USA). Cys-loop mutation ligation products were transformed into DH5α (ThermoScientific ...
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bioRxiv - Microbiology 2023Quote: ... The samples were digested overnight at 37°C with sequencing-grade modified trypsin (Promega) at an enzyme to protein ratio of 1:40 ...
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bioRxiv - Synthetic Biology 2023Quote: ... or zero min at 37°C before addition of Nano-Glo substrate (Promega N1110) in 80 µL ...
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bioRxiv - Cell Biology 2023Quote: ... after which samples were digested overnight at 37 °C with trypsin (Promega, Fitchburg, WI,) in a 1:60 trypsin to protein ratio ...
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bioRxiv - Cell Biology 2024Quote: ... and cut finely prior to in-gel digestion with Trypsin/Lys-C Mix (Promega) at 37 °C for 12 h ...
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bioRxiv - Cancer Biology 2021Quote: Each enhancer or promoter region was amplified from human genomic DNA and cloned into pGL4.10 [luc2] (Promega) containing a SNP (rs718960 ...
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bioRxiv - Neuroscience 2020Quote: ... and pCMVΔ8.9 plasmids were co-introduced into human embryonic kidney 293T cells using the FuGENE reagent (Promega). 48 h after transfection ...
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bioRxiv - Microbiology 2020Quote: ... albicans to damage human vascular endothelial cells was assessed by the CytoTox-96 assay (Promega, Madison, WI), which measures the release of lactate dehydrogenase (LDH ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... cells were transfected with human RXFP1 pcDNA-Zeo-tetO and the GloSensor reporter plasmid using FuGENE (Promega), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... HEK 293T cells were co-transfected with human MOR and a luciferase-based cAMP biosensor (GloSensorTM, Promega). The next day ...
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bioRxiv - Genetics 2022Quote: We performed transient transfection on human pluripotent stem cells using FuGENE® HD Transfection Reagent (Promega, E2311). The cells were splitted and treated with Y-27632 for one before transfection.
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bioRxiv - Cell Biology 2024Quote: All human cell lines were authenticated at each batch freezing by STR profiling (StemElite ID System, Promega). All cell lines were tested for mycoplasma at each batch freezing by PCR (32 ...
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bioRxiv - Genomics 2023Quote: ... Primer efficiency was calculated using a 10-fold serial dilution of Human Mixed Genomic DNA (Promega G3041). For primer sequences and calculated efficiencies ...
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bioRxiv - Molecular Biology 2023Quote: ... 15 mg of His-Tev-HaloTag-(3C)-human γ-TuNA was coupled to Halo Magne beads (Promega). Xenopus laevis egg extract was prepared by standard methods28 ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins in the excised band were digested with Trypsin (Promega, V5280). The resulting peptides were analyzed online by nano-flow HPLC-nanoelectrospray ionization using a Qexactive HFX mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2020Quote: TNT® SP6 High-Yield Wheat Germ Protein Expression System (Promega) was used to perform in vitro translation assay ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were digested with 10ng/ul of sequencing grade trypsin (Promega) overnight at 37C ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were then digested using modified trypsin (Promega, P/N V5113) at a 1:20 (w/w ...
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bioRxiv - Microbiology 2021Quote: ... Crosslinked proteins were digested by the addition of 1μg trypsin (Promega) and overnight incubation at 30°C ...
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bioRxiv - Neuroscience 2020Quote: ... The protein digestion was performed by adding 0.2 µg LysC (Promega) in 50 µL of 100 mM ABC for 3h at 1200 rpm at 37°C followed by overnight trypsin digestion using 0.2 µg of trypsin (Promega ...
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bioRxiv - Neuroscience 2020Quote: ... Fusion proteins were expressed by TnT® Quick Coupled system (Promega). 15 μL of HA tagged protein was then incubated with candidate GST-coated beads at 4°C for 2 hours ...
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bioRxiv - Cell Biology 2021Quote: ... Protein digestion was carried out with sequencing grade modified Trypsin (Promega) at 1/50 protease/protein (wt/wt ...
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bioRxiv - Biochemistry 2021Quote: ... the proteins were digested by addition of sequencing-grade trypsin (Promega) at an enzyme-to-substrate ratio of 1:25 ...
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bioRxiv - Biochemistry 2020Quote: ... The proteins were digested with 500 ng trypsin (Promega, Madison, USA) overnight at 37°C on a shaking incubator ...
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bioRxiv - Plant Biology 2021Quote: ... Expressed proteins were directly captured using Magne Halo Tag Beads (Promega). The protein-bound beads were incubated with adaptor-ligated gDNA fragments ...
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bioRxiv - Cell Biology 2021Quote: ... Then the protein was digested with sequencing grade modified trypsin (Promega) (1 ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were then digested with 12.5 ng/uL modified trypsin (Promega) in 50 μL 100 mM ammonium bicarbonate ...
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bioRxiv - Biochemistry 2022Quote: ... proteins were digested in-gel using trypsin (modified, sequencing purity, Promega), as previously described (Casabona et al. ...
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bioRxiv - Plant Biology 2022Quote: ... anti-GFP (Abkine A02020) and Magnetic Protein G Beads (Promega, G747A) overnight at 4°C with gentle agitation ...
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bioRxiv - Systems Biology 2022Quote: ... Proteins were digested with mass spec grade Trypsin/LysC mix (Promega) at a 1:50 (w/w ...
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bioRxiv - Biochemistry 2022Quote: ... Protein samples were mixed with Trypsin Gold (Promega, Madison, WI, USA) at a 50:1 ratio (w/w ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were digested with trypsin overnight (0.12 μg Trypsin Gold [Promega UK Ltd ...
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bioRxiv - Plant Biology 2021Quote: ... Enzymatic digestion of proteins using LysC/Trypsin Mix (Promega, Fitchburg, WI) was then performed according to the technical manual ...
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bioRxiv - Physiology 2021Quote: ... and overnight protein digestion using 4 μg MS-grade trypsin (Promega) at 37°C ...
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bioRxiv - Biochemistry 2020Quote: The protein samples were digested with trypsin (Promega, mass spectrometry grade) in (NH4)HCO3 buffer (0.1 M ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were then digested with 12.5 ng/µL modified trypsin (Promega) in 50 µL 100 mM ammonium bicarbonate ...
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bioRxiv - Cell Biology 2022Quote: Bead-bound protein was digested with 100 ng trypsin (Promega, V5113) overnight at 37 °C ...
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bioRxiv - Microbiology 2019Quote: ... Other samples were processed using protein precipitation solution (A795A; Promega, France) [12] ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were then in-gel digested using trypsin (sequencing grade, Promega). Resulting peptides were analyzed by online nanoLC-MS/MS (Ultimate 3000 RSLCnano and Q-Exactive Plus or HF ...
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bioRxiv - Systems Biology 2019Quote: ... protein was digested in 1.65 µg of sequencing-grade trypsin (Promega) for 6 hours at 37°C ...
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bioRxiv - Genetics 2020Quote: ... 40 μg of extracted proteins were fragmented by trypsin digestion (Promega Sequencing Grade Modified Trypsin V5111) ...
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bioRxiv - Molecular Biology 2020Quote: ... Halo-tag containing proteins were purified using Magnetic HaloLink Resin (Promega) at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were then subjected to trypsin digestion (Promega; Madison, WI, USA) at trypsin:protein ratio of 1:50 at 37°C ...
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bioRxiv - Developmental Biology 2022Quote: ... Purified protein was then digested into peptides using Trypsin (Promega, V5111) and desalted using Pierce C18 Spin Columns (Thermo Fisher ...
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bioRxiv - Biochemistry 2021Quote: ... 11S protein was detected with a rabbit polyclonal antibody from Promega, and Tom40 with a rabbit polyclonal antibody produced by Cambridge Research Biochemicals (Billingham ...
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bioRxiv - Microbiology 2020Quote: ... and then the proteins were digested to peptides using trypsin (Promega) at a trypsin/protein ratio of 1:50 (w/w ...