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Citations for Promega :
1201 - 1250 of 4304 citations for Human Immunodeficiency Virus Tat Protein HIV 1 Clade C TV019 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... Proteins were digested over night by the addition of trypsin (Promega) and samples were acidified by using formic acid according to [29] ...
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bioRxiv - Microbiology 2023Quote: ... proteins were digested in-gel using modified trypsin (sequencing purity, Promega), as previously described [51] ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were digested on-column with 0.75 µg of trypsin (Promega) in 50 mM TEAB pH 8.5 overnight at 37°C in a humidified incubator ...
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bioRxiv - Cancer Biology 2023Quote: ... 30μg of proteins were digested with 1µg Trypsin sequencing grade (Promega) for 14h at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were then digested with sequencing grade trypsin (Promega, Madison, WI) at 37 °C overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were digested with endoproteinase LysC (Wako Chemicals) and trypsin (Promega) overnight at 37°C ...
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bioRxiv - Plant Biology 2023Quote: ... proteins were digested in-gel using trypsin (modified, sequencing purity, Promega), as previously described [88] ...
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bioRxiv - Microbiology 2023Quote: ... and the protein solution was digested with 0.5 µg trypsin (Promega) overnight at room temperature ...
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bioRxiv - Plant Biology 2023Quote: ... 300 mM NaCl and protein inhibitor cocktail (Promega, Madison, Wisconsin, USA) at pH 7.4 ...
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bioRxiv - Microbiology 2023Quote: ... After 24h the cells were harvested using protein lysis buffer (Promega) and centrifuged for 10min at 13,000rpm ...
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bioRxiv - Cell Biology 2023Quote: Proteins were cleaved with the trypsin/endoproteinase LysC mixture (Promega, V5072) using the paramagnetic bead-based method (54) ...
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bioRxiv - Neuroscience 2023Quote: ... 50 ug of protein per sample was digested with trypsin (Promega) overnight at 37°C using S-TRAP micro cartridges (Protifi LLC,) ...
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bioRxiv - Synthetic Biology 2023Quote: ... His-tag proteins have been purified by using MagneHis system (Promega) and DynaMag spin magnet (Thermo Fisher Scientific) ...
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bioRxiv - Genomics 2023Quote: ... We added Nano-Glo Luciferase Assay Substrate and LgBiT protein (Promega) and imaged using the ImageQuant LAS4000 Imager.
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were in-gel digested using 1µg trypsin (Trypsin Gold, Promega)(37) ...
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bioRxiv - Cancer Biology 2023Quote: ... proteins were digested in-gel using modified trypsin (sequencing grade, Promega), as previously described 58 ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were eluted from beads using partial on-bead trypsination (Promega) followed by a second digestion ...
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bioRxiv - Genetics 2024Quote: ... Proteins were immediately solubilized in 8 M urea and tryptic (Promega) digestion was performed overnight at 37°C with shaking followed by desalting ...
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bioRxiv - Immunology 2024Quote: Recombinant antibodies were purified using protein G magnetic beads (Promega #G7472), HiTrap protein G HP columns (Cytiva #17040401) ...
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bioRxiv - Neuroscience 2024Quote: ... Protein digestion was carried out with sequencing grade modified trypsin (Promega) at 1/50 protease/protein (wt/wt ...
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bioRxiv - Immunology 2023Quote: Proteins were digested with sequencing-grade trypsin (Promega, Fitchburg, MA, USA). Each sample was analyzed by nanoLC-MS/MS on a QExactive+ mass spectrometer coupled to an EASY-nanoLC-1000 (Thermo-Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... the purified protein was incubated with 50 µM Halo-Alexa488 (Promega) for 10 minutes at room temperature ...
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bioRxiv - Genetics 2021Quote: ... then a 5 min final extension (72 °C)—using GoTaq Green Master Mix (M7123, Promega) and the pCFD6_seqfwd and pCFD6_seqrev primers (Table S1 ...
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bioRxiv - Biophysics 2022Quote: ... BRET measurements were performed at 37°C by the addition of furimazine (Promega, Wisconsin, USA) at a dilution of 1:200 ...
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bioRxiv - Genomics 2020Quote: ... and in-gel digested overnight at 37°C with sequencing grade trypsin (Promega Cat# V5111). The peptides were then extracted with extraction buffer (50% acetonitrile (Fisher Chemical Cat#A955-4) ...
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bioRxiv - Genetics 2019Quote: ... following manufacturer’s protocol at 50°C in the presence of 2 U/μl RNasin (Promega). RT negative controls in which the reverse transcriptase enzyme was omitted were set up in parallel ...
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bioRxiv - Biochemistry 2019Quote: ... Plates were incubated for 30 min at 25 °C before NanoBRET Nano-Glo Substrate (Promega) was added to both control and experimental samples at a final concentration of 10 µM ...
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bioRxiv - Biophysics 2021Quote: ... The purified RNA was treated for 30 min at 37°C with RQ1 DNase (Promega) according to the manufacturer’s protocol and then purified using one round of each phenol/chloroform and chloroform extraction followed by precipitation using ethanol in presence of 300 mM sodium acetate pH 5.5 and GlycoBlue coprecipitant (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: ... Resuspended grindate was incubated (4°C, 20 min) with 660 U of DNase I (Promega). Insoluble cell debris was pelleted by spinning (16,000 g ...
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bioRxiv - Microbiology 2021Quote: ... Samples were digested overnight at 37 °C with 5 µg sequencing-grade modified trypsin (Promega). The samples were then acidified with trifluoroacetic acid (TFA ...
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bioRxiv - Microbiology 2021Quote: ... The concentrates were treated for 30 min at 37°C with 10μL of DNAse (Promega) and 2,5μL of RNAse (Macherey-Nagel ...
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bioRxiv - Cell Biology 2020Quote: ... Cultures were then incubated overnight at 4°C with either anti-β3-tubulin (Promega #G712A) or anti-Oct4 (Santa Cruz Biotechnologies #SC-5279 ...
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bioRxiv - Cell Biology 2020Quote: ... and incubated overnight at 37°C with 0.5 µg of sequencing grade modified trypsin (Promega). Samples were acidified using 1% TFA and desalted using 200 µL StageTips packed with three Empore™ SPE Disks C18 (3M ...
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bioRxiv - Biophysics 2022Quote: ... In-gel proteolytic digestion was performed overnight at 37°C using sequencing-grade trypsin (Promega). The resulting peptides were extracted at room temperature with 50% acetonitrile/0.1% trifluoroacetic acid ...
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bioRxiv - Developmental Biology 2022Quote: ... and incubated at 37° C for 30 minutes with RQ1 RNase-Free DNase (Promega, M6101). cDNA synthesis was performed using random primers (Promega ...
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bioRxiv - Neuroscience 2020Quote: ... After two days incubation at 37°C cell viability was assessed using CellTiterGlo (Promega, G7570) following manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Proteolytic digestion was initiated by the addition of 250 ng of endoproteinase Lys-C (Promega) and incubating at room temperature for 2 hours ...
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bioRxiv - Cell Biology 2019Quote: ... RT) and sequentially digested with Lys-C (Wako) (o/n at RT) and trypsin (Promega) (6 h at 37 °C) ...
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bioRxiv - Cell Biology 2019Quote: ... Samples were digested at 37°C overnight in 10 μg MS-grade Trypsin Gold (Promega). Sequencing grade trifluoroacetic acid (TFA ...
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bioRxiv - Biochemistry 2021Quote: ... selections were performed using biotinylated c-CDCP1-Fc captured on SA-coated magnetic beads (Promega). Prior to each selection ...
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bioRxiv - Cancer Biology 2020Quote: ... rat C/EBP□-LIP or -LAP containing pcDNA3 or pSV2Zeo vector by using FugeneHD (Promega) according to the manufactures protocol ...
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bioRxiv - Biochemistry 2022Quote: ... smegmatis Complex I (8 μg) was digested with MS-grade trypsin/Lys-C mix (Promega) at a 25:1 (w/w Complex I:protease ratio in 25 mM Tris-HCl ...
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bioRxiv - Biochemistry 2022Quote: ... 1B and transferred to 37°C immediately after addition of supercoiled pGemT target DNA (Promega). Reactions were incubated for 30 ...
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bioRxiv - Plant Biology 2022Quote: ... DNA was incubated at 80°C for 5 min and mixed with FuGENE HD (Promega) in a ratio of 3:1 (FuGENE:DNA ...
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bioRxiv - Cell Biology 2022Quote: ... and then digested overnight with 300 ng of Trypsin/Lys-C Mix (Promega, Madison, WI). For on-bead digestion following SA pull-down ...
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bioRxiv - Cell Biology 2022Quote: ... The gel pieces were digested at 37°C initially with 0.5 µg of trypsin (Promega) per sample for 6 hours then additionally with 0.3 μg of trypsin overnight ...
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bioRxiv - Molecular Biology 2022Quote: ... Each fraction was digested for 15–18h at 37°C using ultra-grade Trypsin (Promega) at a 1:100 enzyme ...
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bioRxiv - Genomics 2023Quote: ... at 42°C for 8 hours in the presence of RNasin Ribonuclease Inhibitor (Promega N2111) and Pyrophosphatase (NEB M0361S) ...
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bioRxiv - Cancer Biology 2023Quote: ... we added 500 ng of trypsin/Lys-C Mix (CAT# V5072, Promega, Madison, WI, USA) to the beads soaked in 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2023Quote: ... Trypsin digestion was performed at 37 °C with 0.66 µg Trypsin Platinum (Promega, Madison, WI) overnight ...