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Citations for Promega :
1151 - 1200 of 4570 citations for Human Immunodeficiency Virus Tat Protein HIV 1 Clade C TV019 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2023Quote: ... and catalytic hydrolysis of proteins was initiated by trypsin (Promega) at a weight ratio of 1:50 protease:substrate ...
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bioRxiv - Systems Biology 2023Quote: ... and catalytic hydrolysis of proteins was initiated by trypsin (Promega) at a weight ratio of 1:50 protease:substrate ...
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bioRxiv - Systems Biology 2023Quote: ... proteins were digested using Sequencing Grade Modified Trypsin (Promega - V5111) with a 1:50 ratio (enzyme:protein ...
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bioRxiv - Microbiology 2023Quote: ... Protein digestion was achieved with 2.5 µg of trypsin (Promega) and overnight incubation at 37°C ...
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bioRxiv - Microbiology 2023Quote: The protein was digested overnight with 0.1 μg LysC (Promega) and 0.8 μg trypsin (Thermo Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... the proteins were digested by trypsin (Promega, Madison, WI, USA), and peptides were extracted from the gel with an acetonitrile/formic acid solution ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were enzymatically digested with 0.5 ug Trypsin Gold (Promega) for 16 h ...
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bioRxiv - Genomics 2023Quote: ... Proteins were digested with modified trypsin (sequencing grade, porcine, Promega) in enzyme:protein ratio of 1:50 overnight at 37 °C ...
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bioRxiv - Genetics 2023Quote: ... proteins in the plugs were digested overnight with trypsin (Promega) at 37°C in a 25-mM NH4HCO3 buffer (0.2 µg trypsin in 20 µL) ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentration was determined using Bradford Assay Kit (Promega, USA) and equal amounts of proteins were denatured ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were digested overnight with sequencing-grade modified trypsin (Promega). Then ...
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bioRxiv - Cell Biology 2023Quote: ... proteins were digested with 6 ng/µL trypsin (Promega, UK) overnight at 37 °C ...
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bioRxiv - Plant Biology 2023Quote: ... alkylation (55 mM CAA) and protein digestion (trypsin-gold, Promega) the resulting peptides were purified using self-packed StageTips (C18 material ...
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bioRxiv - Bioengineering 2024Quote: ... several concentrations of purified recombinant HaloTag protein standard (Promega, G4491) were labeled with ligand and run in duplicate to generate a calibration curve ...
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bioRxiv - Cancer Biology 2024Quote: ... The proteins were then digested by trypsin (sequencing-grade, Promega) at 1:50 ratio at 37 °C overnight ...
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bioRxiv - Developmental Biology 2024Quote: ... and digested according to the In-solution Protein Digestion (Promega) protocol ...
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bioRxiv - Molecular Biology 2020Quote: Human Genomic DNA was extracted from the blood using the Wizard Genomic DNA Purification kit (Promega) as per the instructions.
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bioRxiv - Immunology 2021Quote: ... 25 μl of 7.5×104 Jurkat effector cells expressing either human FcγRIIIa or murine FcγRIV (Promega) resuspended in assay buffer were added to each well ...
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bioRxiv - Molecular Biology 2021Quote: Full-length human RNU6 sequence (110 nucleotides) was amplified by PCR and cloned into psiCHECK2 (Promega), downstream of Renilla luciferase ...
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bioRxiv - Neuroscience 2021Quote: Genomic DNA (gDNA) was extracted from human frontal cortex using Wizard Genomic DNA Purification Kit (Promega), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Standards for quantification were created by serial dilution of pre-quantified BSC human male DNA (Promega). Amplification reactions were performed in duplicate using 96 well-plates using a CFX96 Touch Real-time PCR Detection System (Bio-Rad).
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bioRxiv - Cancer Biology 2020Quote: The region of interest (750-850 bp) was PCR amplified from pooled male human DNA (Promega) and cloned into a STARRseq luciferase validation vector_ORI_empty plasmid (Addgene plasmid #99298 ...
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bioRxiv - Molecular Biology 2020Quote: ... WT or mutant promoter of human Rpl28 gene was cloned into pGL-3-basic (Promega, E1751). The number is relative to transcriptional start site ...
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bioRxiv - Bioengineering 2020Quote: ... and detected with anti-rabbit or anti-human HRP-conjugated secondary antibodies (Promega W4011 and W4031). Blots were developed using ECL (ThermoScientific 32106 ...
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bioRxiv - Immunology 2024Quote: ... 3×106 ADCC and ADCP Bioassay Effector Cells expressing the human FcγRIIIa or FcγRIIa receptors (Promega) were added per well and the cells were incubated in at 37°C with 5% CO2 for 6 hours ...
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bioRxiv - Immunology 2023Quote: ... 100 μl of 5,000-fold diluted Peroxidase AffiniPure goat anti-human IgG (H+L) antibody (Promega) was added into each well and incubated for 1 h at 37 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... Human and murine NLRC5 and CXCL10 promoters were cloned into pGL3 basic luciferase reporter vector (Promega). All mutated plasmids were constructed from wild type plasmids by Fast Mutagenesis System (TransGen Biotech) ...
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bioRxiv - Cell Biology 2023Quote: ... 10mM Ultrapure ATP and 0.7 μg of recombinant full-length human CDC7/DBF4 kinase (Promega, V5088). Reactions were incubated at 37 °C for 120 min and then terminated by freezing on dry ice before mass spectrometry.
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bioRxiv - Synthetic Biology 2019Quote: ... Poly-C tails were added to the fragmented DNA using terminal deoxynucleotidyl transferase (Promega). Then ...
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bioRxiv - Microbiology 2021Quote: ... Urea was diluted to a final concentration of 1.5M and trypsin/Lys-C (Promega) added to a ratio of 25:1 protein:trypsin ...
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bioRxiv - Cell Biology 2020Quote: ... 20 µg aliquots of Trypsin/Lys-C (MS grade; Promega Corporation, Madison, WI, USA) were dissolved in 400 µl 50 mM TEAB and 20 µg of this solution was added directly onto the resin of the filter (corresponding to 1 µg Trypsin/Lys-C per sample ...
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bioRxiv - Biochemistry 2022Quote: ... N-or C-terminal NanoLuc/Kinase fusions were encoded in pFN31K expression vectors (Promega), including flexible Gly-Ser-Ser-Gly linkers between Nluc and each full-length kinase ...
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bioRxiv - Neuroscience 2022Quote: ... Total RNA was reverse transcribed at 42°C by using GoScript Transcription System (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2019Quote: ... Destined and dried fragments were re-swelled with Trypsin/Lys-C Mix solution (Promega) overnight in a humidified chamber at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... and then ligated for 16 h at 4 °C with T4 DNA ligase (Promega). After a further purification step ...
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bioRxiv - Molecular Biology 2021Quote: ... Rat Gβ1 was cloned with a C-terminal SmBiT34 (peptide 86 or HiBiT, Promega) connected with a 15AA polypeptide linker ...
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bioRxiv - Microbiology 2021Quote: ... cDNA was synthesized at 42 °C for 60 min with M-MLV-RT (Promega) using gene-specific primer SP1_nbdA ...
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bioRxiv - Synthetic Biology 2021Quote: ... Samples were digested overnight at 37 °C using Trypsin Gold (Mass Spectrometry Grade, Promega). After digestion ...
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bioRxiv - Cell Biology 2021Quote: ... The samples were then diluted to 4M urea and digested with Lys-C (Promega), 67:1 (protein ...
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Dual and Opposing Roles for the Kinesin-2 Motor, KIF17, in Hedgehog-dependent Cerebellar DevelopmentbioRxiv - Developmental Biology 2022Quote: ... for 15 minutes at 37°C before lysing with reporter lysis buffer (Promega, E397A). Samples were spun at 15,000 rpm for 10 min at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... The samples were then digested by Lys-C (Princeton Separations) and trypsin proteases (Promega) (Florens et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... 2ul/5-10mg DW of Trypsin/Lys-C (Endoproteinase LysC) Mix (Promega, Cat #V5071) was added to samples and incubated with continuous agitation at 150 rpm for overnight at 37°C ...
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bioRxiv - Physiology 2022Quote: ... Peptides contained C-terminal biotinylations and were spotted on streptavidin-conjugated membranes (Promega #V2861) and imaged with Typhoon FLA 7000 phosphorimager (GE ...
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bioRxiv - Molecular Biology 2022Quote: ... samples were digested overnight at 37°C with 10 nG MS Grade Trypsin (Promega). Trypsinization was quenched with 1 μL of 0.1% Trifluoroacetic acid (TFA ...
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bioRxiv - Cell Biology 2024Quote: ... biological replicates (n=7) were digested with Lys-C (Alpha Laboratories) and trypsin (Promega) on beads as previously described (Hubner et al. ...
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bioRxiv - Cancer Biology 2024Quote: ... for 10 minutes at 37°C in the presence of RNasin Ribonuclease Inhibitor (Promega). DNase-treated RNA was purified with the RNA Clean and Concentrator-25 Kit (Zymo Research ...
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bioRxiv - Biochemistry 2024Quote: ... The NanoLuc coding sequence including linker was obtained from the pNLF1-C plasmid (Promega) and inserted at the C-terminus of the TDO2 gene sequence (Flash Therapeutics ...
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bioRxiv - Cell Biology 2023Quote: ... The samples were then diluted to 3M urea and digested with Lys-C (Promega) for 4 h at 25°C ...
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bioRxiv - Microbiology 2023Quote: ... Plates were stored at -20°C before adding 100 μl Steady Glo substrate (Promega) to perform a luciferase assay as described earlier ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the assay was performed at 20 °C using a Glomax 20/20 luminometer (Promega) by the luciferin/luciferase system supplemented with 25 µM P1,P5-di(adenosine-5’ ...