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Citations for Promega :
1051 - 1100 of 4570 citations for Human Immunodeficiency Virus Tat Protein HIV 1 Clade C TV019 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... GST-Halo was used as a protein standard (Promega). Incubate 1 μL of reticulocyte extract before binding with beads and 2 μL of extract after the binding was used for each western on a precast gel (4–20% Criterion™ TGX™ Precast Midi Protein Gel ...
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bioRxiv - Plant Biology 2020Quote: Eluted proteins were digested with sequencing-grade trypsin (Promega) and analysed by nanoLC-MS/MS on a QExactive+ mass spectrometer coupled to an EASY-nanoLC-1000 (Thermo-Fisher Scientific ...
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bioRxiv - Molecular Biology 2019Quote: Eluted proteins were digested with sequencing-grade trypsin (Promega) and analyzed by nanoLC-MS/MS on a QExactive+ mass spectrometer coupled to an EASY-nanoLC-1000 (Thermo-Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were digested with 300ng trypsin (Trypsin Gold, Promega) in 3µl 50mM ammonium bicarbonate followed by incubation at 37°C overnight ...
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bioRxiv - Developmental Biology 2021Quote: ... Proteins were digested with 0.25µg of Trypsin/LysC (Promega) at a 1:100 enzyme/substrate ratio overnight at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: Eluted proteins were digested with sequencing-grade trypsin (Promega) and analyzed by nanoLC-MS/MS ...
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bioRxiv - Cell Biology 2022Quote: ... Complexed proteins were eluted in SDS Elution Buffer (Promega) for 30 minutes at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... and proteins were in-gel digested with trypsin (Promega), five fractions per sample ...
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bioRxiv - Molecular Biology 2024Quote: ... The proteins were digested on-beads with Trypsin (Promega) with ratio enzyme:proteins 1:20 in 50 mM ammonium bicarbonate and performed overnight at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were then digested with 0.1 μg trypsin (Promega) at 37°C overnight ...
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bioRxiv - Microbiology 2023Quote: ... proteins were digested overnight with 0.63 µg trypsin (Promega). Tryptic digestion was stopped by adding formic acid to a final concentration of 1.8% (v/v) ...
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bioRxiv - Genetics 2023Quote: ... Eluted proteins were digested with sequencing-grade trypsin (Promega). Peptide mixtures were separated using Evosep One (Evosep Biosystems ...
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bioRxiv - Microbiology 2023Quote: ... enriched proteins were eluted by adding 1µg trypsin (Promega) and incubation for 30min at 30°C ...
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bioRxiv - Synthetic Biology 2023Quote: ... using the HiBiT Control Protein (20 µM, N3010, Promega) as a standard.
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bioRxiv - Microbiology 2023Quote: ... Proteins were digested using sequencing grade modified trypsin (Promega) with a ratio of 1:50 (enzyme:protein ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were digested with 1µg Trypsin sequencing grade (Promega) for 14h at 37°C ...
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bioRxiv - Immunology 2022Quote: ... Proteins were subsequently digested with 15μg Trypsin Gold (Promega) in 50mM ammonium bicarbonate (Sigma-Aldrich ...
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Flaviviruses alter endoplasmic reticulum-mitochondria contacts to regulate respiration and apoptosisbioRxiv - Microbiology 2023Quote: ... and the protein solution was digested with trypsin (Promega) overnight at room temperature ...
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bioRxiv - Immunology 2023Quote: ... Eluted proteins were then treated with PNGase F (Promega) according to manufacturer’s protocol and analyzed with SDS-PAGE ...
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bioRxiv - Cell Biology 2023Quote: ... proteins were in-gel digested with trypsin (Promega, V5113). Tryptic peptides were purified by STAGE tips prior to LC-MS/MS measurements ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were digested with 500 ng of trypsin (Promega) and LysC (Wako Chemicals) ...
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bioRxiv - Microbiology 2024Quote: ... and proteins were digested with LysC and trypsin (Promega) in two steps with a final enzyme-to-protein ratio of 1:25 ...
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bioRxiv - Biochemistry 2024Quote: ... Protein was digested using sequencing-grade trypsin (Promega, V511) at 1:50 (20 µg trypsin:1 mg protein ...
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bioRxiv - Genetics 2024Quote: ... Proteins were reduced and treated with Trypsin Gold (Promega). The peptide abundance of each sample was measured and each sample was labeled with non-isobaric mass tags ...
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bioRxiv - Microbiology 2024Quote: ... and the protein solution was digested with trypsin (Promega) overnight at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... lentiviral packaging vectors into human HEK-293T cells via calciumphosphate transfection (Promega; Madison, WI, USA), according to manufacturer’s directions ...
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bioRxiv - Cancer Biology 2021Quote: The 3’UTR of human PAQR3 was cloned into pmirGLO vector (Promega, Madison, WI, USA), and then validated by sequencing ...
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bioRxiv - Molecular Biology 2021Quote: ... Human SMARCB1 was translated in vitro using TNT Quick Coupled Transcription/Translation System (L1170, Promega). 1 µg of pcDNA3.1-FLAG-SMARCB1 was incubated at 30°C for 90 minutes with 20 µM methionine and TNT T7 Quick Master Mix ...
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bioRxiv - Cancer Biology 2020Quote: ... Human cell lines were DNA fingerprinted for provenance using the Power-Plex 1.2 kit (Promega) and confirmed to be the same as the DNA fingerprint library maintained by ATCC ...
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bioRxiv - Bioengineering 2021Quote: The open reading frame for human SOX17 was PCR amplified using GoTaq Master Mix (Promega) from the PB-TRE3G-SOX17 plasmid (Table S5) ...
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bioRxiv - Genetics 2022Quote: ... human POPDC1 and POPDC2 cDNA sequences were cloned into the pFC14K or pFC32K plasmids (Promega), which contain C-terminus sequences for HaloTag and NanoLuc tags ...
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bioRxiv - Immunology 2024Quote: ... supernatant was removed for immediate analysis using the LumitTM HMGB1 (Human/Mouse) Immunoassay (Promega, #W6110). Luminescent samples were read in opaque white 96-well plates using a CLARIOstar plate reader (BMG Labtech).
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bioRxiv - Plant Biology 2021Quote: ... United States The HY5 protein was expressed with TnT® SP6 High-Yield Wheat Germ Protein Expression System (Promega, Madison, Wisconsin, United States) using 2 μg Plasmid DNA per 50 μL expression reaction ...
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bioRxiv - Cell Biology 2022Quote: ... The protein concentration was measured using Pierce™ BCA Protein Assay Kit and GloMax® Explorer Multimode Microplate Reader (Promega, Madison, WI, USA). Expression of OAT1 ...
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bioRxiv - Molecular Biology 2023Quote: ... Bacterial lysates expressing fused GST-CuLCrV / SiGMV / TYLCV CP proteins and GST control were incubated with MagneGST□ particles (cat#V8600, MagneGST protein purification system, Promega, Madison, WI, USA) to immobilize the GST tagged proteins as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... alkylation was performed using 20 mM 2-chloroacetamide at room temperature in HEPES buffer for 30 min under exclusion of light. Samples were prepared according to the SP3 protocol (Hughes et al. 2019) and trypsinized (sequencing grade, Promega, enzyme to protein ratio 1:50) overnight at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... Digestion was carried out overnight at 37°C using Trypsin/LysC mix (Promega) at a protein/enzyme ratio of 50:1 in a ThermoMixer under continuous mixing at 1000 rpm ...
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bioRxiv - Cell Biology 2021Quote: ... Their masses as well as masses of their theoretical Lys-C protease (Promega) fragments ...
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bioRxiv - Cancer Biology 2020Quote: ... Triplicate biological replicates were digested with Lys-C (Alpha Laboratories) and trypsin (Promega) on beads as previously described68 ...
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bioRxiv - Biochemistry 2022Quote: ... Trypsin and trypsin/Lys-C proteases (MS grade) were from Promega (Madison, WI); RapiGest detergent from Waters (Eschborn ...
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bioRxiv - Synthetic Biology 2021Quote: ... We also included N-terminal and C-terminal HiBiT tags (Promega, Madison, WI) for quantification using luminescence ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... + 0.1 μg of NSD2 PWWP1-NanoLuc® Fusion Vector DNA (C-terminal) (Promega) using X-tremeGENE HP DNA Transfection Reagent (Sigma) ...
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bioRxiv - Molecular Biology 2024Quote: ... and digestion was allowed to proceed overnight at 37°C using trypsin (Promega) and LysC (Wako ...
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bioRxiv - Cell Biology 2024Quote: ... and GST-C-WRN were affinity-purified using glutathione (GSH)-magnetic beads (Promega).
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bioRxiv - Plant Biology 2023Quote: Two vials of trypsin/Lys-C mix (100µg, V5078, Promega, Alexandria, NSW, Australia) were dissolved into 1 mL of the resuspension buffer (50mM acetic acid ...
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bioRxiv - Molecular Biology 2023Quote: Ni-NTA beads were firstly digested with 500 ng recombinant Lys-C (Promega) at RT while shaking at 1,400 rpm ...
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bioRxiv - Cell Biology 2023Quote: ... Mass spectrometry grade Trypsin or Trypsin/Lys-C Mix (Promega #V5111 or V5071) was added at to a final concentration of 1µg/ml and incubated for 30 min at 37°C in the flask ...
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bioRxiv - Biochemistry 2023Quote: ... 10 µL of 2 ng/µL trypsin/Lys-C Mix (CAT# V5072, Promega) in 0.3% acetic acid was added to a 96-well plate (CAT# 0030128664 ...
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bioRxiv - Immunology 2023Quote: The eluted HSA-IgG complexes were digested by Lys-C and trypsin (Promega) to peptides and analyzed by LC-MS/MS via HCD combined with electron-transfer/higher-energy collision dissociation (EThcD ...
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bioRxiv - Cancer Biology 2022Quote: ... Quadruplicate biological replicates were digested with Lys-C (Alpha Laboratories) and trypsin (Promega) on beads as previously described 60 ...