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Citations for Promega :
1001 - 1050 of 4570 citations for Human Immunodeficiency Virus Tat Protein HIV 1 Clade C TV019 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... pH 8.5 to a final urea concentration of 2 M for Trypsin/Lys-C based overnight protein digestion at 37 °C (40 µg of protein used for global proteomics and the remainder for phosphoproteomics, 1:70 protease: substrate ratio, Mass Spectrometry grade, Promega Corporation, Cat No: V5072.) Digestions were acidified with trifluoroacetic acid (TFA ...
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bioRxiv - Plant Biology 2021Quote: ... The pSPUTK promoter allowed in vitro protein synthesis using the TnT® SP6 High-Yield Wheat Germ Protein Expression System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... and human 293T cells as producer cells using the FUGENE HD transfection reagent (Promega), as described [50] ...
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bioRxiv - Pathology 2019Quote: ... human primary aortic SMCs were transfected with pGL4.34 Vector plasmids (E1350; Promega, Madison, WI) using Effectene Transfection Reagent (301425 ...
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bioRxiv - Neuroscience 2022Quote: Open reading frames of human OR genes were subcloned into pCI (Promega, WI, USA) with a Rho-tag (the sequence encoding the first 20 amino acids of rhodopsin ...
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bioRxiv - Biochemistry 2024Quote: ... 24 hours after transient transfection with mouse or human ENPP3 via Fugene 6 (Promega), the media was gently removed and replaced with serum-free DMEM supplemented with 1% insulin-transferrin-selenium-sodium pyruvate (ThermoFisher ...
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bioRxiv - Immunology 2023Quote: ... tailed with complete TRAC and TRBC1 gene segments amplified from human genomic DNA (Promega), respectively ...
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bioRxiv - Pathology 2020Quote: ... the 60°C annealing/extension temperature recommended by the mastermix manufacturer (Promega) produced more robust amplification (data not shown).
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bioRxiv - Biochemistry 2021Quote: ... and finally digested overnight at 37 °C with 375 ng Trypsin (Promega). The peptides were extracted from the gel twice using 100 μl of 50 % acetonitrile and 0.25 % TFA buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... and 30 s at 68°C or through GO taq polymerase (Promega) for 2 min at 95°C followed by 25 cycles of 30 s at 95°C ...
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bioRxiv - Cell Biology 2022Quote: ... Proteolytic digestion was carried out with a trypsin/Lys-C mixture (Promega) incubated for 18 h at 37°C at a protein to protease ratio of 25:1 ...
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bioRxiv - Neuroscience 2021Quote: ... the plasmid was re-ligated overnight at 16°C (ligase from Promega) and transform in Stbl4 bacteria ...
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bioRxiv - Microbiology 2020Quote: ... The samples were treated with trypsin/Lys-C protease mix (Promega, V5072) at a protease:protein ratio of 1:25 for 16 h at 37°C ...
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bioRxiv - Biochemistry 2021Quote: ... For digestion Typsin/Lys-C mass spectrometry grade proteases mixture from Promega Corporation was used ...
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bioRxiv - Neuroscience 2022Quote: ... Bands digested overnigt at 37 °C with 800 ng of trypsin (Promega) and/or chymotrypsin (Promega ...
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bioRxiv - Neuroscience 2022Quote: ... in-gel digestion was performed using 0.10 µg trypsin/Lys-C (Promega) overnight in 25 mM NH4HCO3 at 30 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... Histones were digested in 50 mM ammonium bicarbonate using Arg-C (Promega), and the resulting peptides desalted by C18 StageTip 86 ...
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bioRxiv - Biochemistry 2024Quote: ... Trypsin/Lys-C Mix (mass spectrometry grade) was from Promega (Madison, WI). Glu-1-Fibrinopeptide B was from Waters (Milford ...
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bioRxiv - Neuroscience 2024Quote: ... each well was supplemented with 0.32µg MS grade Trypsin/Lys-C (Promega) in 50 mM NH4HCO3 and incubated overnight at 37 °C within a humidified incubator ...
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bioRxiv - Biochemistry 2023Quote: ... followed by 500 ng of trypsin/Lys-C Mix (CAT# V5072, Promega), and mixed gently at 37°C overnight to digest proteins ...
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bioRxiv - Bioengineering 2023Quote: ... and digested for 2 hours at 50°C using Trypsin Platinum (Promega) using 1:50 Trypsin to sample ratio by mass ...
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bioRxiv - Cell Biology 2022Quote: ... The samples were digested with sequencing grade trypsin/lys C enzyme (Promega) using manufacture recommended protocol ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... samples were digested with 0.1 μg of trypsin/Lys-C mix (Promega) at 37 °C for 1 hour ...
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bioRxiv - Cancer Biology 2023Quote: ... and samples were digested with sequencing grade Trypsin/Lys-C enzyme (Promega) in the presence of 10% Acetonitrile for 16 hours at 37°C ...
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A synthetic elastic protein as molecular prosthetic candidate to strengthen vascular wall elasticitybioRxiv - Cell Biology 2023Quote: ... SEP was digested overnight at 37°C with sequencing grade pepsin (Promega) at a 1/20 (pepsin/SEP ...
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bioRxiv - Evolutionary Biology 2024Quote: ... incubation with 0.5 μg of Trypsin/Lys-C Mix (Promega, Madison, WI) at room temperature for 3 h ...
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bioRxiv - Molecular Biology 2024Quote: ... Promega NanoBiT plasmid backbone (pBiT_1.1-C[TK/LgBiT], Promega, Madison, WI, USA) was combined with overlapping fragments generated by PCR which encoded for i ...
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bioRxiv - Microbiology 2024Quote: ... Samples were then digested with 4 μg of Trypsin/lys-C (Promega) overnight at 37°C before being collected by centrifugation with washes of 100mM Tetraethylammonium bromide ...
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bioRxiv - Cancer Biology 2022Quote: ... Each sample was then diluted 10-fold with 50 mM ammonium bicarbonate for digestion with 0.375 µg (for 15 µg protein samples) or 0.125 µg (for 5 µg protein samples) sequencing grade modified porcine trypsin (Promega, Madison, WI, USA). The samples were incubated at 45°C for 1 hour in a chilled microwave (CEM ...
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bioRxiv - Cancer Biology 2021Quote: ... The proteins were digested with trypsin (Promega Corporation, USA) at an enzyme-to-substrate ratio of 1:50 (w/w ...
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bioRxiv - Molecular Biology 2021Quote: ... 30 μg of protein were digested with trypsin (Promega) in two steps (2 h at 37 ºC with 1:40 trypsin:protein ratio ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was then digested with Trypsin Gold (Promega) 1:100 at 37 °C overnight in 10 mM ammonium bicarbonate pH 8.0 and the resultant peptides were cleaned up with POROS 20 R2 (Applied Biosystens) ...
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bioRxiv - Immunology 2019Quote: ... Protein material was digested with 600 ng Trypsin (Promega) overnight at 37°C at 300 rpm ...
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bioRxiv - Developmental Biology 2019Quote: ... Precipitated proteins were subjected to on-bead trypsin (Promega) digestion ...
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bioRxiv - Bioengineering 2021Quote: ... Proteins were digested with sequencing grade modified trypsin (Promega) at an enzyme to substrate ratio of 1:50 overnight at RT ...
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bioRxiv - Bioengineering 2020Quote: ... Proteins were digested with sequencing grade modified trypsin (Promega) at an enzyme to substrate ratio of 1:50 overnight at RT ...
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bioRxiv - Cell Biology 2021Quote: ... with that of the Halo 7-tag protein (Promega), with the insertion of a 63-base linker (21 amino acids ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were digested overnight with LysC/Trypsin mix (Promega) and sequencing-grade modified Trypsin (Promega ...
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bioRxiv - Neuroscience 2019Quote: ... Protein was digested with sequencing grade modified trypsin (Promega) using the FASP (Filter Aided Sample Preparation ...
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bioRxiv - Microbiology 2019Quote: ... proteins were predigested with 1.5 µg of rLysC (Promega) for 3 h at room temperature and samples diluted with 50 mM ammonium bicarbonate ...
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bioRxiv - Molecular Biology 2021Quote: ... with Broad Range Protein Molecular Weight Markers (V8491, Promega) used as molecular markers ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were harvested in passive lysis buffer 1x (Promega) at indicated times and
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bioRxiv - Plant Biology 2021Quote: Eluted proteins were digested with sequencing-grade trypsin (Promega) and analyzed by nano LCMS/MS on a QExactive + mass spectrometer coupled to an EASY-nanoLC-1000 (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were digested with trypsin (Promega, Madison, Wisconsin, USA) in 40 mM AmBic at 37°C for 18 h (enzyme to protein ratio (v/w ...
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bioRxiv - Molecular Biology 2019Quote: ... Proteins were digested using 0.08 μg Trypsin Gold (Promega) per gel slice at 37°C for 16 h and peptides were extracted from the band pieces using acetonitrile ...
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bioRxiv - Biochemistry 2020Quote: ... proteins were trypsinized overnight (Sequencing Grade Modified Trypsin, Promega, cleaves C-terminal to lysine and arginine residues ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were digested on beads with Trypsin/LysC (Promega) overnight followed by zip-tip cleanup using C-18 Omix tips (Agilent) ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were digested on beads with Trypsin/LysC (Promega) overnight followed by zip-tip cleanup using C-18 Omix tips (Agilent) ...
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bioRxiv - Synthetic Biology 2021Quote: ... A standard curve of HiBiT Control Protein N3010 (Promega) was diluted in PLB+PI at concentration ranging from 0.01 to 2 nM ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were detected with ECL Western Blotting Substrate (Promega) or SuperSignal™ West Femto Maximum Sensitivity Substrate (ThermoFisher ...