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Citations for Promega :
1101 - 1150 of 2354 citations for ssc mir 487b RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... First-strand cDNA was synthesised using 1 µg RNA and 500 ng µl-1 Oligo(dT)15 primer (Promega, Southampton, UK), denatured at 65°C for 5 min ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The 16S rRNA gene was amplified using primers 27F-YM 5’-AGAGTTTGATYMTGGCTCAG-3’ and 1391R 5’-GACGGGCGGTGWGTRCA-3’ and GoTaq DNA Polymerase (Promega, USA). The PCR was performed as follows ...
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bioRxiv - Plant Biology 2023Quote: ... and P5Solexa and P3Solexa primers (Supplementary Data 12) on the whole eluted volume and a second ProNEX purification step (Promega, NG2001) to remove residual primers ...
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bioRxiv - Biochemistry 2024Quote: ... iCLIP2 libraries were pre-amplified with 6 PCR cycles using short Solexa primers P5 and P3 (Suppl. Tab. 9) and then size-selected using the ProNex Size-Selective Purification System (Promega, NG2001) in a 1:2.95 (v/v ...
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bioRxiv - Molecular Biology 2023Quote: ... The PEG10-5’AmC6-modified primers were used for the amplification of the donor template using GoTaq® Green Master Mix DNA polymerase (Promega). The SmUbi-EGFP-SmUbi construct with the 600 bp HA sequence (21 ...
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bioRxiv - Bioengineering 2020Quote: ... amplicons from three PCR runs for each root sample were combined (Clement et al., 1998) and then purified using a PCR purification kit (PCR Clean-up Kit; PROMEGA Inc., Wisconsin, USA).
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bioRxiv - Biochemistry 2021Quote: ... Amplified PCR products were visualized by blue light illumination and the 504/505 bp bands were excised and the amplified PCR product purified using the Wizard SV Gel and PCR Clean-Up kit (Promega, Madison, WI, USA). Extracted DNA was Sanger sequenced at the University of Michigan Advanced Genomics Core and analyzed for presence of the single base pair deletion using DNAStar LaserGene SeqMan Pro 15 ...
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bioRxiv - Molecular Biology 2023Quote: The DNA was extracted using the Promega PCR Cleaning Kit (Wizard® SV Gel and PCR Clean-Up System, Promega Cat.-No. A9285) as indicated in the manual ...
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bioRxiv - Microbiology 2022Quote: ... then dipped in a well of a 96-well PCR plate filled with 20 µl of a gyrB PCR mix (4 µl of 5x GoTaq buffer (Promega, Madison, WI, USA), 0.4 µl of 10mM dNTP ...
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bioRxiv - Molecular Biology 2020Quote: First strand synthesis was performed in 25 μl reaction volumes using 5 μl of MMLV-RT 5X buffer (Promega, Wisconsin, UK), 1.25 μl of dNTPs (10mM ...
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bioRxiv - Microbiology 2021Quote: ... was added to each well and incubated for 1 h at RT before fluorescence intensity was read on a Glomax® Explorer Fully Loaded (Promega) with emission wavelengths of 500-550 nm and an excitation wavelength of 475 nm and graphs were generated using GraphPad Prism (V.8.4.1) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Real-Time PCR Diagnostic Panel17 and a protocol for quantifying the SARS-CoV-2 subgenomic E gene RNA (E SgRNA)18 using the GoTaq® Probe 1-Step RT-qPCR System (Promega). For quantification of SARS-CoV-2 using the nCoV assay ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Real-Time PCR Diagnostic Panel17 and a protocol for quantifying the SARS-CoV-2 subgenomic E gene RNA (E SgRNA)18 using the GoTaq® Probe 1-Step RT-qPCR System (Promega). For quantification of SARS-CoV-2 using the nCoV assay ...
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bioRxiv - Plant Biology 2021Quote: ... RT-qPCR assays were carried out in triplicate using SYBR® Green chemistry with GoTaq® qPCR Master Mix (Promega, UK) on a StepOne™ Real-Time PCR Machine (Applied Biosystems ...
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bioRxiv - Microbiology 2022Quote: ... then diluted 1:5 with nuclease-free water before 2 µL was used as template in a 10 µL GoTaq 1-Step RT-qPCR (Promega, A6020) reaction (69) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Amplification reactions were run in a 20 μL volume using the GoTaq 1-Step RT-qPCR System (Promega, Madison WI, USA) and the recommended manufacturer’s instructions as described in Cervera et al ...
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bioRxiv - Microbiology 2020Quote: ... samples were heated at 80 °C for 15 min and cooled to RT before addition of 1 μg of trypsin (Promega, V511A). Samples were digested at 37 °C overnight ...
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bioRxiv - Microbiology 2023Quote: The viral RNA derived from the lung and nasal turbinate samples was quantified using a protocol for quantifying the SARS-CoV-2 sub-genomic E gene RNA (sgE)29 using the GoTaq® Probe 1-Step RT-qPCR System (Promega).
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bioRxiv - Molecular Biology 2021Quote: ... 5’int-F and 24nt-6stp-R primers and 2) 3’KI: 24nt-6stp-F and 3’int-R using the GoTaq DNA polymerase mix (Promega, Madison, WI) and 200 nM of each primer ...
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bioRxiv - Molecular Biology 2019Quote: ... PCR was performed using primers spanning 75-150 base pairs (Table S5) for each transcript using GoTaq® Green Master Mix (Promega, M7122) for 35 cycles.
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bioRxiv - Cell Biology 2022Quote: ... DNase-treated RNA was transcribed into complementary DNA (cDNA) using the ImProm-II™ reverse transcription system and oligo-dT as primer (Promega, Germany). Quantitative real-time reverse transcriptase polymerase chain reaction (qPCR ...
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bioRxiv - Biochemistry 2022Quote: The 4330bp PCR product amplified from M13mp18 by using primers: oM13-5-27 and oM13-3-24 was inserted into pGEM®-T Easy(PROMEGA #A137A). The resultant plasmid is named as pM13 ...
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bioRxiv - Physiology 2019Quote: ... Complementary DNA (cDNA) was synthesized from 1.2 μg RNA using random primers and the GoScript™ Reverse Transcription System (Promega, Mannheim, Germany) at 25°C for 5 min ...
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bioRxiv - Microbiology 2020Quote: ... OXA-111-NcoI F or OXA-71-NcoI F and OXA-66-XhoI R primers (Table 2) and TA cloned into the vector pGEM-T Easy (Promega, United Kingdom). The inserts were confirmed by sequencing with the universal T7 Promoter primer ...
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bioRxiv - Biophysics 2020Quote: ... First-strand cDNA was synthesized from 2 μg of total RNA with oligo (dT)15 primers and GoScript™ Reverse Transcriptase (Promega, Switzerland) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... was synthesized from 1.0 µg of total RNA using MMLV reverse transcriptase and a cocktail of oligo dT and random primers (Promega, Madison, WI, USA). qPCR was performed using PowerSYBR green PCR master mix (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA was synthesized with primers specific for each strand separately at 50 °C (Supplemental Table S1) using the ImProm-II™ Reverse Transcription System (Promega, A3800) and subjected to qPCR with primers specific for the viral N-protein (Supplemental Table S1).
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bioRxiv - Neuroscience 2023Quote: ... 1ug of total RNA extracted from transfected SK-N-MC cells was reverse transcribed using the ImProm-II Reverse Transcription System and random hexamer primers (Promega, Madison, MI). PowerUp SYBR™ Green Master Mix (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... A fragment of the rat Piezo 1 cDNA was amplified with Taq Polymerase and the oligonucleotide primers 5’-GAGGAAGAGGACTACCTT and 5’-TTTACTTAGAAAACCCTACAG from bladder total RNA and cloned into the pGEM-T Easy vector (Promega, Madison, WI). Sequence was confirmed by Sanger capillary sequencing and sense and antisense RNA probes were synthesized with T7 and SP6 RNA polymerases (Roche-Sigma-Aldrich) ...
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bioRxiv - Physiology 2024Quote: ... target cDNA fragments were amplified with the primers listed in Table 1 and ligated into pGEM T-Easy vector (Promega, Madison, WI). The cDNA fragments were PCR amplified with M13 primers and purified with the FastGene Gel/PCR Extraction Kit (Nippon Genetics ...
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bioRxiv - Microbiology 2024Quote: ... Complementary DNA (cDNA) synthesis was performed with oligo-dT primers and random hexamers using the First Strand cDNA Synthesis Kit (Promega, Madison WI) following the manufacturer’s instructions.
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Throat colour polymorphism in relation to sex and body size of the Litter skink, Lankascincus fallaxbioRxiv - Zoology 2022Quote: ... The PCR amplification was carried out in 25 μl volumes employing 35 cycles with an annealing temperature of 55 °C following standard PCR protocols with Promega PCR master mix (Promega Corporation, Madison, Wisconsin, USA). The success of the PCR amplification of each sample was confirmed through Gel electrophoresis using 5 μl of each sample ...
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bioRxiv - Molecular Biology 2023Quote: ... Japan) after PCR amplification and extrication from the agarose gel using Wizard® SV Gel and PCR Clean-Up System (Promega, Madison, WI, USA). The threshold was set to 0.6 ΔRn ...
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bioRxiv - Evolutionary Biology 2020Quote: ... the PCR products were cloned using the pGEMT kit (Promega): 3 μl PCR products were ligated to 50 ng pGEMT vector in the presence of 3 units T4 ligase overnight at 18°C (final volume 10 μL) ...
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bioRxiv - Neuroscience 2022Quote: ... PCR products were ligated into pGEMT.easy (Promega, Madison, WI, US) and sequenced with Sanger sequencing ...
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bioRxiv - Molecular Biology 2020Quote: PCR reactions were carried out using Go-Taq enzyme (Promega) with 1 μl of cDNA diluted 1:5 ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR was performed using GoTaq G2 DNA Polymerase (Promega, USA) according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2019Quote: ... purified chromatin was recovered using a PCR purification kit (Promega). Sequencing libraries were generated using standard methods and samples were sequenced on a MiniSeq instrument (Illumina ...
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bioRxiv - Zoology 2019Quote: ... purified using Wizard SV Gel and PCR Cleanup kit (Promega), and sequenced directly (Sequetech ...
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bioRxiv - Microbiology 2019Quote: ... PCR products were cloned into pGEM-T Easy vector (Promega) and sequenced using T7 promoter forward primer by Sanger sequencing ...
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bioRxiv - Genetics 2019Quote: ... The PCR products were cloned into pGEM-T easy (Promega) and the sequence of the resulting plasmids was verified ...
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bioRxiv - Developmental Biology 2019Quote: ... PCR products cloning into pGEM-T-easy vector system (Promega). We Sanger sequenced 10 colonies per sample using M13 primers using the above described method and results were analysed with BISMA online tool (Bisulfite Sequencing DNA Methylation Analysis - http://services.ibc.uni-stuttgart.de/BDPC/BISMA/ ...
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bioRxiv - Genetics 2020Quote: ... The PCR is carried using GoTaq DNA polymerase (Promega, UK) and the following PCR conditions ...
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bioRxiv - Developmental Biology 2021Quote: ... PCRs were conducted using the GoTaq Master Mix (Promega Corporation), and products were run on a 1% agarose gel in 1X TAE Buffer.
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bioRxiv - Neuroscience 2021Quote: ... and PCR was performed using GoTaq green master mix (Promega). Primers used were ...
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bioRxiv - Plant Biology 2020Quote: ... or Wizard SV Gel and PCR Clean-Up System (Promega), and then sequenced.
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bioRxiv - Microbiology 2021Quote: ... a PCR fragment was amplified using GoTaq DNA polymerase (Promega) and subcloned in a TOPO cloning vector (Invitrogen) ...
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bioRxiv - Cancer Biology 2020Quote: ... PCR amplification was performed using GoTaq Green Master Mix (Promega) or Phusion High-Fidelity DNA polymerase (NEB ...
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bioRxiv - Synthetic Biology 2022Quote: ... PCR products were purified prior to quantification via QuantiFluor (Promega) and combined into an equimolar pool ...
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bioRxiv - Neuroscience 2020Quote: ... PCR products were cloned by A-tailing into pGEMTeasy (Promega) and sequenced ...