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Citations for Promega :
1051 - 1100 of 5339 citations for ssc mir 411 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Fluorescence of FITC-dextran in serum was diluted to 1:10 with PBS and measured on a Promega GloMax Multi Detection System (Promega) at 490 nm excitation and 520 nm emission wavelengths ...
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bioRxiv - Neuroscience 2022Quote: ... supernatant was collected and protein concentration quantified using the 660 nm Protein Assay Reagent (Pierce) supplemented with 50 mM Ionic Detergent Compatible Reagent (Pierce) in a GloMax Multi Detection Plate Reader (Promega). After boiling in 1X Laemmli Sample Buffer (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... a rabbit polyclonal antibody was used followed by detection with a goat anti-rabbit secondary antibody conjugated to horseradish peroxidase and developed using a chemiluminescent substrate (Promega). Immunoblot detection of 6xHIS and 2A peptide tags were determined through anti-Histidine tag (AD1.1.10 ...
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bioRxiv - Immunology 2023Quote: ... single cell clones were first screened for insertion of an in-frame repair template using the Nano-Glo HiBit Lytic Detection System (Promega). HiBit-positive clones were then expanded and screened by anti-CD19 Western blot and genomic DNA PCR for genotyping.
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bioRxiv - Molecular Biology 2023Quote: The bioluminescence resonance energy transfer (BRET) ubiquitination assay was performed using the NanoBRET Nano-Glo Detection Systems (Standard) (N1661, Promega) according to the manufacturer’s guidelines ...
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bioRxiv - Plant Biology 2023Quote: ... Protein yield was quantified by measuring luminescence with the Nano-Glo® HiBiT Extracellular Detection System (N2420, Promega, Madison, WI) and HiBiT Control Protein (N3010 ...
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bioRxiv - Neuroscience 2023Quote: Surface expression was measured using a HiBit-tagged 5-HT2A receptor and the Nano-Glo HiBit Extracellular Detection System (Promega). N-terminal HiBit-tagged human 5-HT2A receptor was cloned into pcDNA3.1 using Gibson Assembly ...
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bioRxiv - Microbiology 2023Quote: ... and LgBiT Protein (1:100)/HiBiT Lytic Substrate (1:50) in Nano-Glo HiBiT Lytic Buffer (25 μl) (Nano-Glo HiBiT Lytic Detection System; Promega) were mixed and incubated for 10min at room temperature according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... 10 μg of the protein lysates were diluted to 50 μL using the M-PER buffer followed by complementation with LgBiT using Nano-Glo HiBiT Lytic Detection System (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... and bacterial bioluminescence was measured in relative luminescence units (RLUs) every 24 h for up to seven days using a GloMax Multi Detection System (Promega) (35) ...
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bioRxiv - Molecular Biology 2023Quote: ... Luciferase activity was measured with a GloMax®-Multi Detection system using the Dual-Glo® Luciferase Assay System (Promega). Results were analyzed using Excel (Microsoft ...
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bioRxiv - Genomics 2023Quote: ... Cells were harvested 24 hours after transfection and luciferase measured using Luciferase Assay System and the GloMax Multi-Detection System (Promega). For Verteporfin (VP ...
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bioRxiv - Molecular Biology 2024Quote: ... the amount of fluorescence of supernatant and solubilized cells was measured with a Glomax multi + detection system (Promega, WI, USA). The efflux capacity was calculated from the ratio of fluorescent intensity in the supernatant and the total fluorescent intensity from the supernatant and cell lysate ...
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bioRxiv - Immunology 2021Quote: ... RT-qPCR to detect ZIKV was performed in a 20 μL reaction final volume containing GoTaq one-step RT-qPCR master mix (Promega), 25 ng of sample RNA ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were subjected to digestion of Lys-C (Wako Chemicals) (1:50) for 2 h at RT and then to trypsin (Promega) (1:50 ...
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The AAA-ATPase ATAD1 and its partners promote degradation of desmin intermediate filaments in musclebioRxiv - Cell Biology 2022Quote: ... modified with 10mM Iodoacetamide in 100 mM ABC (at the dark, RT, 30 mins) and digested in 10% Acetonitrile and 10 mM ABC with modified trypsin (Promega) at a 1:10 enzyme-tosubstrate ratio overnight at 37°C ...
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bioRxiv - Molecular Biology 2019Quote: ... 5 μl of Reverse Transcription Mix (10 μl of 10 mM dNTPs and 40 μl of 5X MMLV RT Buffer Promega), 1 μl of 10 mM ddTTP for the sequencing reaction and 1 μl of MMLV Reverse Transcriptase RNAse H minus (Promega ...
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bioRxiv - Synthetic Biology 2019Quote: ... Backbone and inserts were ligated either for 3 h at RT or overnight at 4°C using T4 ligase (Promega). All vector sequences (Table S5 ...
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bioRxiv - Plant Biology 2020Quote: ... The concentrations of the resulting cDNAs were adjusted to 10 ng/µL and 2 µL were used for RT-qPCR reactions performed with the GoTaq qPCR Master Mix (Promega) in a final volume of 10 µL ...
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bioRxiv - Molecular Biology 2020Quote: ... Resulting ribosomal complexes were analyzed by toe-printing as described (Pisarev et al., 2007) using avian myeloblastosis virus reverse transcriptase (AMV RT) (Promega) and [32P]-labeled oligonucleotide primers complementary to HalV nt ...
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bioRxiv - Immunology 2022Quote: ... Cells were then incubated at 37°C for 72 h after which they were lysed for 20 min on a shaker platform at RT by addition of Reporter Lysis Buffer (Promega). Luciferase signal was determined from cell lysates by adding the Bright-Glo Luciferase buffer (Promega ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 μL of the annealed oligos were ligated with 0.04 ng digested PspCAs13b crRNA backbone in 10 μL of T4 ligation buffer (3 h, RT) (Promega, M1801). All PspCas13b crRNA spacer sequences used in this study are listed in Supplementary File 1 ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were washed twice with UA buffer and twice with 50mM ammonium bicarbonate prior to digest of the immobilized proteins on the filter for 2 h at RT using 1 mg Lys-C (Wako Chemicals) and for 16 h at 37C using 2 mg trypsin (Promega). Tryptic peptides were collected by centrifugation (10 min at 14,000 g) ...
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bioRxiv - Microbiology 2020Quote: ... The reactions were subjected to primer extension at 42°C for 45 min using 1 unit of MMLV-RT (Promega) and 0.5mM of dNTPs ...
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bioRxiv - Developmental Biology 2019Quote: ... cells into micro-tubes containing 5 µl of cold 1X SuperScript IV VILO Master Mix for two-step RT-qPCR containing 6 units RNasin (Promega) and 0.5% (v/v ...
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bioRxiv - Microbiology 2022Quote: ... Complementary DNA was synthetized from 500 ng total RNA by using random hexamers and moloney murine leukemia virus (M-MLV) reverse transcriptase (RT) Point Mutant Synthesis System (Promega). Quantification was performed by using the LightCycler 480 Universal Probe Library System (Roche) ...
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bioRxiv - Immunology 2022Quote: ... followed by retrotranscription to cDNA with M-MLV RT, RNase H(–) point mutant (Promegain presence of buffer, nucleotides and RNasin Plus RNase Inhibitor (Promega). Reaction was then incubated at 40 °C for 10 min ...
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bioRxiv - Microbiology 2022Quote: ... and alkylated with 5 mM of iodoacetamide 30 min in the dark at RT°C prior to digestion with 5 μg of trypsin Gold (Promega) for 15 h at 37 °C ...
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bioRxiv - Bioengineering 2023Quote: ... The day after the plates were pre-cooled (RT, 15’) supplemented with luciferase substrate (Bright Glo Luciferase Assay System, Promega) incubated (RT ...
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bioRxiv - Biochemistry 2022Quote: ... RNA was isolated 24 hours after transfection and the pattern of alternative splicing was assessed by RT (with oligo-d(T) / random hexamer) and AMV Reverse Transcriptase (Promega) and PCR with GoTaq (Promega ...
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bioRxiv - Microbiology 2023Quote: ... and then reverse transcribed into complementary DNA (cDNA) using 30 enzyme units(U) of the MMLV-RT (Promega, WI, USA) and 1μM of the labeled primer ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were digested with 2 μg of lysyl endopeptidase (Wako) at RT for overnight and were further digested overnight with 2 μg trypsin (Promega) at RT ...
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bioRxiv - Microbiology 2023Quote: ... proteins were alkylated in 5 mM iodoacetamide for 1 hour in the darkness at RT and digested overnight at 37°C with 1 µg trypsin (Trypsin Gold Mass Spectrometry Grade, Promega). Peptides were recovered by centrifugation ...
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bioRxiv - Neuroscience 2023Quote: ... Blots were then incubated with blotting buffer containing 1:200 LgBiT for 1-2 h with rocking at RT (N2410, Promega). Next ...
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bioRxiv - Immunology 2023Quote: ... cDNA was generated from 20-100 ng of RNA using the GoTaq 2-step RT-qPCR System (Promega, cat# A6110). qPCR was performed with SYBR Green on a StepOnePlus real-time PCR system (Applied Biosystems) ...
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bioRxiv - Microbiology 2023Quote: ... The reactions were subjected to primer extension at 42°C for 45 min using 1 unit of MMLV-RT (Promega) and 0.5 mM of dNTPs ...
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bioRxiv - Cancer Biology 2024Quote: ... 1ug of each RNA sample was reverse-transcribed using Moloney Murine Leukemia Virus reverse transcriptase (M-MLV-RT, Promega, #M1701) and random hexamer primers (Thermo Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... 1 µg of RNA was then used to synthesize cDNA in a 20 µl reverse transcription reaction using 5X First Strand Buffer and MMLV-RT (Promega) with random hexamers ...
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bioRxiv - Cell Biology 2020Quote: ... mCherry and CLIP were PCR amplified from pFN21A (Promega), pSNAPf (NEB) ...
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bioRxiv - Molecular Biology 2021Quote: ... Genotypes were determined by PCR with GoTaq Green (Promega) or allelic discrimination for MommeD1 or MommeD43 ...
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bioRxiv - Cell Biology 2022Quote: ... Wizard SV Gel and PCR Clean-Up System (ProMega) was used on cDNA samples ...
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bioRxiv - Cancer Biology 2019Quote: ... and PCR reactions were performed using GoTaq Green (Promega) using 30 cycles of 94°C for 30 sec ...
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bioRxiv - Microbiology 2019Quote: ... and analysed by PCR with GoTaq Master Mix (Promega) using the following conditions ...
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bioRxiv - Microbiology 2019Quote: ... PCR was performed by using GoTaq Master Mixes (Promega) with the following conditions ...
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bioRxiv - Microbiology 2019Quote: ... 0.2 mM of each dNTP (PCR Nucleotide Mix, Promega), 0.1 μM of each primer ...
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bioRxiv - Cancer Biology 2019Quote: ... Quantitative PCR was performed using SYBR-green mastermix (Promega) and run in a Prism 7900 HT instrument (Applied Biosystems) ...
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bioRxiv - Genetics 2020Quote: ... and 200 ng PCR product using FuGENE HD (Promega). At 2 days post transfection ...
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bioRxiv - Cancer Biology 2021Quote: Transcription templates were synthesized by PCR (GOTAQ G2, Promega) using a hybrid T7promoter/sequence-specific forward primer and a sequence-specific reverse primer ...
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bioRxiv - Cell Biology 2021Quote: ... PCR products were digested with SacI and KpnI (Promega) and ligated in the pZsGreen1-1 plasmid ...
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bioRxiv - Microbiology 2020Quote: PCRs were performed in 25 μl reaction buffer (Promega) with MgCl2 at 1.5mM final ...