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Citations for Promega :
1001 - 1050 of 5339 citations for ssc mir 411 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Firefly luciferase activity was measured after replacing the media with 50µl Bright-Glo luciferase reagent and measuring luminescence on a GloMax-MultiTm Detection system (Promega). Neutralization titers were calculated by interpolating the reciprocal serum dilution at which there was a 50% inhibition of luciferase activity relative to untreated controls (ND50).
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bioRxiv - Cancer Biology 2024Quote: ... Authenticity of cell lines was evaluated by detection of ten genetic loci using the GenePrint® 10 System (Promega) and cross referencing to ATCC or internal genetic profiles.
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bioRxiv - Microbiology 2020Quote: ... Two hundred nanograms of purified RNA were reverse-transcribed using Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV RT, Promega). RT was primed with oligo dT(15 ...
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bioRxiv - Cell Biology 2021Quote: ... Viral RNA was detected using the CDC 2019-Novel Coronavirus Real-Time RT-PCR Diagnostic Panel as per Centre for Disease and Control’s optimised protocol (Integrated DNA Technologies) for use with Go-Taq 1-step RT-qPCR Master Mix (Promega). Gene expression profile and SARS-CoV-2 N subgenomic RNA expression were determined with a standard qPCR cycle (50°C for 2 minutes ...
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bioRxiv - Pathology 2022Quote: ... G490 5 × All-In-One RT MasterMix (abm) was used for reverse transcription according to the manufacturer’s protocol (Promega). The qRT-PCR analysis was performed in 96-well plates using the BIO-RAD CFX96 detection system ...
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bioRxiv - Immunology 2021Quote: ... Reverse transcription was performed with Moloney murine leukemia virus reverse transcriptase (MMLV-RT) according to the manufacturer’s protocol (Promega) using 200 ng of total RNA ...
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bioRxiv - Microbiology 2023Quote: ... RNA reverse transcription and cDNA amplification was then carried out by the GoTaq 1-Step RT-qPCR System (Promega), using established primers (31) ...
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bioRxiv - Neuroscience 2023Quote: ... for 3 hours at RT and then continued overnight with addition of 2 μg of trypsin (Promega, Cat# V5280), at 37 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... 250 ng of DNAsed RNA were retro-transcribed using random hexamer primers with the M-MLV-RT enzyme (Promega). cDNA were amplified with the GoTaq qPCR Master Mix (Promega ...
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bioRxiv - Developmental Biology 2023Quote: ... with 1 ug of RNA and RT-qPCR reactions were performed using GoTaq® qPCR Master Mix (A6002 - Promega). Analyses were carried out in the QuantStudio™ 5 Real-Time PCR System ...
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bioRxiv - Microbiology 2020Quote: ... The beads were then washed 3 times and incubated with 30ul lysis buffer (Promega). Then 15μl of the lysate was used to measure bound NanoLuc activity.
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bioRxiv - Molecular Biology 2020Quote: ... After each incubation time cell viability was determined using the CellTiter-Glo assay (Promega), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell viability was assessed at several time points after seeding using CellTiter-Blue (Promega). Cell viability was compared as percent viability of a corresponding condition using a shRNA directed against the luciferase gene.
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bioRxiv - Cancer Biology 2021Quote: ... Relative cell numbers were quantified at the indicated time points by CellTiter Glo (Promega) for suspension cultures or by alamarBlue (Thermo Fisher ...
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bioRxiv - Biochemistry 2019Quote: ... Cell growth was assayed at the indicated time using the CellTiter Assay System (Promega).
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bioRxiv - Molecular Biology 2021Quote: ... After each incubation time cell viability was determined using the CellTiter-Glo assay (Promega), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were washed three times with PBSTR (PBS containing 0.1% Tween-20 (Promega, 0000395426), 0.1 U/µL RRI (Vazyme ...
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bioRxiv - Cell Biology 2022Quote: ... Viable cells at different time points were measured by MTS assay (Promega, Cat# G3580) with the absorbance at a wave-length of 490 nm.
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bioRxiv - Bioengineering 2023Quote: ... Luminescence was measured for a single time point using the GloMax Navigator System (Promega) and an integration time of 1 s ...
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bioRxiv - Immunology 2023Quote: ... at the indicated time points media was removed and a CellTiter-Glo assay (Promega) was completed following manufacturer’s instructions.
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bioRxiv - Cancer Biology 2023Quote: ... At the indicated time points cellular ATP was measured using CellTiter-Glo® (Promega) and luminescence detected with SynergyTM 2 multi-mode microplate reader (BioTek® Instruments) ...
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bioRxiv - Developmental Biology 2021Quote: ... The PCR reaction mixture was prepared for a 10 μL reaction volume using a GoTaq® DNA Polymerase PCR mix (Promega): 2 μL 5X Green GoTaq® Reaction Buffer ...
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bioRxiv - Developmental Biology 2021Quote: ... The PCR reaction mixture was prepared for a 10 μL reaction volume using a GoTaq® DNA Polymerase PCR mix (Promega): 2 μL 5X Green GoTaq® Reaction Buffer ...
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bioRxiv - Microbiology 2020Quote: ... Duplicate PCR products from the samples were pooled and purified with the Wizard® Gel and PCR Clean-Up System (Promega), ligated and subsequently cloned into E ...
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bioRxiv - Molecular Biology 2020Quote: ... The PCR products were directly sequenced using PCR primers after purification with Gel Clean-up System (Promega Corporation, Madison, WI, USA). All sequencing reactions were done on ABI 3700 DNA automated sequencer with the BigDye chemistry (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... The resulting PCR amplicon from the second-round PCR was gel-purified and ligated to the pGEMT- Easy vector (Promega, USA), as per the manufacturer-recommended protocol (Promega ...
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bioRxiv - Cell Biology 2019Quote: ... were generated using PCR amplification of two DNA fragments and subsequent assembly PCR (using Pfu DNA polymerase from Promega, Mannheim, Germany) with the artificially inserted exon 9 sequence as region for annealing ...
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bioRxiv - Microbiology 2023Quote: ... Each PCR reaction with a total volume of 25 μL contained 12.5 μL of PCR master mix (Promega; Madison, WI, USA), 8.0 μL of nuclease free water ...
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bioRxiv - Plant Biology 2023Quote: Stem-loop PCR and 5’-RLM-RACE PCR amplification products were purified using The Wizard® SV Gel and PCR Clean-Up System (Promega). Cloning of the different PCR products was performed as described in the pGEM®-T Vector system according to the instructoŕs instructions (Promega) ...
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bioRxiv - Microbiology 2023Quote: ... The three PCR fragments were ligated by PCR using primers P1/P3 and cloned into pGEM®-T easy vector (Promega). Plasmid was then digested with NotI ...
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bioRxiv - Biochemistry 2023Quote: ... The linearized pMRNAXP vector and the PCR fragments of DNA encoding spike were cleaned up by Wizard® SV Gel and PCR Clean-Up System (Promega). The PCR fragments of spike protein DNA were cloned into linearized pMRNAXP vector using In-Fusion® HD Cloning Kit (Clontech® Laboratories ...
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bioRxiv - Genetics 2019Quote: The target genomic region was amplified using PCR and purified using Wizard SV Gel and PCR Clean-Up System (Promega, Madison, WI). The DNA sequence of the PCR fragment was determined using cycle sequencing with BigDye v3.1 reagents (Applied Biosystems ...
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bioRxiv - Evolutionary Biology 2023Quote: ... one microliter volumes of Reverse Transcription reactions were used as template for amplification of internal cDNA fragments using in 20 μl volume PCR reactions (35 rounds of amplification using Promega 2X PCR Master Mix (Promega, Madison, WI). Gene-specific primers and annealing temperatures are listed in Table 1 ...
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bioRxiv - Plant Biology 2023Quote: ... The 16S rDNA PCR-amplified fragments were purified by Wizard® SV Gel and PCR Clean-Up System (Promega, New Delhi, India) and digested by HaeIII (SRL ...
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bioRxiv - Cancer Biology 2019Quote: ... diluted 1:5000 in 5% milk in TBST were used for detection by the addition of an enhanced chemiluminescence (ECL) substrate (Promega). Western blot quantification was performed using Fiji software (Schindelin et al. ...
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bioRxiv - Cell Biology 2019Quote: ... 10 μl of lysate was transferred to a black 96-well Optiplate using 50 μl of Firefly and 50 μl of Renilla detection reagents (reagents were either from Promega or home-made ...
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bioRxiv - Cancer Biology 2020Quote: ... and 50 μl of diluted media added to a white 96 well plate (CLS3610) before the addition of 50 μl of Lactate/Glucose detection reagent (Promega). Plates were incubated for 1 h at RT and luminescence recorded (Glowmax Luminometer ...
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bioRxiv - Microbiology 2020Quote: ... and LgBiT Protein (1:100)/HiBiT Lytic Substrate (1:50) in Nano-Glo HiBiT Lytic Buffer (25 μl) (Nano-Glo HiBiT Lytic Detection System; Promega) were mixed and incubated for ten minutes at room temperature according to the modified manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and LgBiT Protein (1:100)/HiBiT Lytic Substrate (1:50) in Nano-Glo HiBiT Lytic Buffer (25 μl) (Nano-Glo HiBiT Lytic Detection System; Promega) were mixed and incubated for ten minutes at room temperature according to a modified version of the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Luciferase assays were performed on 20 μl of lysate or 1x Cell Culture Lysis Reagent in 96 well plates on the GloMax-Multi Detection System (TM297, Promega). 100 μl Luciferase Assay Reagent was added to wells ...
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bioRxiv - Cell Biology 2021Quote: ... The amount of HiBiT proteins in the concentrated EV fraction was measured using the Nano Glo HiBiT Lytic Detection System (Promega). EV marker proteins in the concentrated EV fraction were detected by western blotting as described above.
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bioRxiv - Microbiology 2022Quote: ... The plate was incubated in the dark for 2 min and then read on a Glomax Multi+ Detection System (Promega) as above ...
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bioRxiv - Cell Biology 2021Quote: The quantification of dead cells in lung tissue was performed using an in-situ apoptosis detection DeadEnd Colorimetric TUNEL System (Promega) as previously described (88) ...
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bioRxiv - Microbiology 2020Quote: ... and LgBiT Protein (1:100)/HiBiT Lytic Substrate (1:50) in Nano-Glo HiBiT Lytic Buffer (25 μl) (Nano-Glo HiBiT Lytic Detection System; Promega) were mixed and incubated for ten minutes at room temperature according to the modified manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2019Quote: ... The supernatant was taken to measure the amount of secreted HiBiT peptide using the Nano-Glo HiBiT Extracellular Detection System (Promega).
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bioRxiv - Synthetic Biology 2019Quote: ... and the supernatant was taken to measure the amount of secreted HiBiT peptide using the Nano-Glo HiBiT Extracellular Detection System (Promega). The resulting luminescence signals were normalized based on the cell viability that was measured using the PrestoBlue Cell Viability Reagent (Thermo).
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bioRxiv - Synthetic Biology 2021Quote: Absolute protein concentration of HiBiT-tagged proteins was measured using the Nano-Glo® HiBiT Extracellular Detection System N2420 (Promega). CFPS reactions were diluted 104-106 -fold using 1X PLB+PI buffer (generated by mixing 2 mL of 5x Passive Lysis Buffer E1941 (Promega ...
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bioRxiv - Microbiology 2021Quote: Luminescence measurements were performed using percoll-enriched infected erythrocytes in 384 well microplates using the Nano-Glo HiBiT extracellular detection system (Promega). Cells were resuspended at 0.2% hematocrit in culture medium diluted with two volumes of 200:1:50 buffer:LgBiT:Furimazine ...
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bioRxiv - Cancer Biology 2020Quote: ... Induction of ISRE-Luc reporter was evaluated by measuring Firefly and Renilla luciferase activity with dual-luciferase reporter assays on Glomax-Multi Detection System (Promega). The results were presented as relative light units (RLU) ...
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bioRxiv - Biochemistry 2021Quote: The ATPase activity of ATP13A2 in microsomes was measured using a luminescence-based ADP detection assay (ADP Glo Max assay; Promega). Reactions were performed in a final volume of 5 µL containing 50 mM MOPS-KOH pH 7.0 ...