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Citations for Promega :
1001 - 1050 of 1580 citations for GATOR2 complex protein WDR24 WDR24 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were digested overnight at 37°C with 2 µg trypsin (Trypsin Gold, Mass spectrometry Grade, Promega). Digestion was stopped by adding 70 µl 1% trifluoroacetic acid (TFA ...
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bioRxiv - Systems Biology 2024Quote: ... Protein digestion was carried out overnight at 37°C with sequencing grade trypsin (Promega, Madison, WI, USA) at 1:40 (w/w ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were digested in-gel overnight at 37°C with Sequencing Grade Modified Trypsin (Promega; cat. #V5111) diluted in 200uL of digestion buffer (40 mM ammonium bicarbonate ...
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bioRxiv - Cell Biology 2020Quote: ... Secondary antibodies were 1:5,000 HRP-conjugated anti-rabbit (W4011; Promega) or mouse (W4021 ...
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bioRxiv - Biophysics 2020Quote: ... monoclonal antibody against β-tubulin III (Promega Corporation, Madison, WI, USA); for astrocytes ...
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bioRxiv - Neuroscience 2020Quote: ... Blots have been developed using horseradish peroxidase-conjugated secondary antibodies (Promega) and the ECL chemiluminescence system (Amersham) ...
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bioRxiv - Neuroscience 2021Quote: ... JF-549 or Halo-tag antibody (1:500, Promega, mouse monoclonal) and measuring the newly synthesized actin (in most cases ...
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bioRxiv - Neuroscience 2021Quote: ... Immunofluorescence utilizing primary antibodies directed against βIII-tubulin (1:1000, Promega) and secondary antibodies Alexa Fluor-488 (1:500 ...
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bioRxiv - Plant Biology 2021Quote: ... Blots were developed with horseradish peroxidase (HRP)-linked secondary antibodies (Promega) and Immobilon Western Chemiluminescent HRP Substrate (Merck).
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bioRxiv - Molecular Biology 2022Quote: ... Washes and incubation of HRP-conjugated secondary antibodies (1:5000, Promega) were performed in PBST (0.05% Tween 20 in PBS) ...
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bioRxiv - Cell Biology 2022Quote: ... followed by incubation with their respective HRP-conjugated secondary antibodies (Promega). The membrane was soaked with Chemi-Lumi Super (Nakarai Tesque ...
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bioRxiv - Microbiology 2022Quote: ... and an anti-mouse IgG HRP conjugate secondary antibody (Promega, #W4021) (13) ...
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bioRxiv - Biophysics 2020Quote: ... The bead-antibody complexes were captured using a magnetic stand (Promega) and the suspensions were removed ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were then incubated with an HRP-conjugated secondary antibody (Promega) for one hour ...
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bioRxiv - Genetics 2020Quote: ... Alkaline phosphatase-conjugated secondary antibodies were used at 1:10000 (Promega). Western blot band intensity was analyzed with ImageJ Gel quantification tool.
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bioRxiv - Cell Biology 2021Quote: ... Primary antibodies used were anti-p75NTR (Promega, Cat: G323A, 1:300) and anti-GAPDH (Sigma ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by the anti-rabbit HRP-conjugated secondary antibody (#W4011; Promega).
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bioRxiv - Microbiology 2022Quote: ... and verified by Western blotting with the anti-HaloTag antibody (Promega). Genomic DNA was extracted from JP2-270 using DNAiso (Takara ...
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bioRxiv - Microbiology 2024Quote: ... Mouse antibodies (CV801, CV804, CV820, CV925, CV1117) and effector cells (Promega) expressing mouse FcγRIV and NFAT luciferase reporter were added to the cells ...
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bioRxiv - Molecular Biology 2024Quote: ... Rabbit anti-HaloTag® polyclonal antibody (G9281) was obtained from Promega. Mouse anti-tubulin monoclonal antibody (66031–1-Ig ...
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bioRxiv - Cell Biology 2023Quote: ... The following antibodies were used: HaloTag mAB (Promega, G9211, 1:500), GAPDH (Cell Signaling 2118 ...
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bioRxiv - Cancer Biology 2023Quote: ... Membrane-bound antibodies were detected by the enhanced chemiluminescence system (Promega). Primary antibodies were rabbit anti-EGFR (Cell Signaling Technology #4267 ...
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bioRxiv - Immunology 2023Quote: ... All secondary HRP-conjugated antibodies were obtained from Promega (Madison, WI). For immunofluorescence only ...
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bioRxiv - Molecular Biology 2023Quote: ... Mouse Anti-LgBiT monoclonal antibody was purchased from Promega (cat. #N7100), HRP-linked anti-mouse IgG was used as a secondary antibody and purchased from Cell Signaling Technology (7076S) ...
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bioRxiv - Biochemistry 2023Quote: ... The following primary antibodies were used: NanoLuc (N7000, Promega, 1:1000), β-ACTIN (#4970S ...
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bioRxiv - Biochemistry 2024Quote: ... To resolve bands recognized by the phospho-p38 antibody (Promega #V1211), a NuPAGE™12% Bis-Tris SDS-PAGE gel (Invitrogen Fisher NP0349BOX ...
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bioRxiv - Cell Biology 2024Quote: ... mouse monoclonal anti-β-galactosidase antibody (1:250, Promega, Cat# Z3781), rat monoclonal anti-Elav (7E8A10 ...
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bioRxiv - Bioengineering 2022Quote: ... 3 mM CaCl2 at pH 6.4) 0.2 µg/μL protein was mixed with proteases trypsin (Promega sequencing grade), chymotrypsin (BD) ...
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bioRxiv - Molecular Biology 2021Quote: ... Reduced and alkylated gel pieces were processed for in-gel protein digestion by trypsin (Promega, Madison WI, USA) overnight at 37 °C adding ammonium bicarbonate buffer to cover gel matrix ...
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bioRxiv - Microbiology 2020Quote: ... dengue protein NS2B/3/4A constructs were expressed in rabbit reticulocyte lysate (TNT coupled T7, Promega Bio Systems) programmed with 1 µg DNA/50 µL and labeled with 20 µCi of L-[35S]-methionine (EasyTag ...
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Multimodal tubulin binding by the yeast kinesin-8, Kip3, underlies its motility and depolymerizationbioRxiv - Biophysics 2021Quote: ... The protein was fluorescently labeled with 10uM TMR (dimer constructs) or 30uM TMR (monomer constructs, HALO-TMR; Promega) on beads for 1h at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... the bead-bound protein was incubated with either 6.7 μM HaloTag-TMR or HaloTag-PEG-biotin ligand (Promega) for 15 minutes at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... and the amount of the HiBiT protein was measured using the Nano Glo HiBiT Lytic Detection System (Promega). As a quantification standard for HiBiT proteins ...
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bioRxiv - Microbiology 2021Quote: ATPase activity of the purified proteins was determined using two kits – Enliten ATPase assay (FF2000, Promega, Moscow, Russia) and malachite green phosphate assay (MAK307 ...
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bioRxiv - Microbiology 2022Quote: ... and SV40 late poly(A) signal was amplified from pNL1.1.CMV[Nluc/CMV] (19.1 kDa NanoLuc protein, Promega). The PCR product was blunt end ligated to pAD1 digested with EcoRV ...
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bioRxiv - Plant Biology 2022Quote: ... His-tagged and GST-tagged recombinant proteins were purified using Promega MagneHis™ Ni-Particles (Promega Cat#V854A) and MagneGST™ Glutathione Particles (Promega Cat# V861A ...
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bioRxiv - Biochemistry 2022Quote: ... the beads with bound recombinant proteins were blocked for 1 h at 4°C using reticulocyte lysate (Promega). Next ...
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bioRxiv - Microbiology 2021Quote: ... sample-filters were incubated in a wet-cell adding 1:50 w/w trypsin: protein ratio (Promega, V5111) solubilized in 120 µl 50 mM TEAB ...
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bioRxiv - Biochemistry 2021Quote: ... and S30 Premix Plus are components of the S30 T7 High-Yield Protein Expression System purchased from Promega. The pEXP5-PCFT plasmid is the PCFT construct shown in Fig ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were digested by incubation with either sequencing-grade modified trypsin (1/50, w/w; Promega, Madison, Wisconsin), chymotrypsin sequencing grade (1/50 ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were extracted using 100 μL of Passive Lysis Buffer from Dual-Luciferase reporter assay systems (Promega, E1980) according to manufacturer’s instruction and assayed using FLUOstar Omega (BMG Labtech) ...
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bioRxiv - Molecular Biology 2022Quote: ... the proteins were digested overnight at 37 °C with 6 ng μL-1 of Asp-N (Promega, UK). Peptides were extracted in 2 % v/v formic acid ...
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bioRxiv - Microbiology 2022Quote: ... Proteins on magnetic beads were digested overnight at 37°C with 1μl (0.2 μg/μL) of trypsin (Promega) in a 25-mM NH4HCO3 buffer per sample ...
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bioRxiv - Biochemistry 2020Quote: ... Lep proteins were then produced from mRNA by in vitro translation using rabbit reticulocyte lysate (Promega, Madison, WI) supplemented with canine pancreatic rough microsomes (tRNA probes ...
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bioRxiv - Microbiology 2020Quote: The protein samples were digested with 500 ng of sequencing grade trypsin (V511, Promega Co., Madison, WI, USA) and incubated at 37 °C following the protocol described by Shevchenko et al.22 ...
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bioRxiv - Plant Biology 2020Quote: ... LUC activity was measured with 20 μl of protein sample and 100 μl of LUC assay reagent (Promega). Activation from the DHS was calculated as (LUC luminescence/rate of MUG accumulation ...
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bioRxiv - Cell Biology 2020Quote: ... The proteins were then digested overnight at 37°C with 0.2 μg of modified sequencing grade trypsin (Promega) in 50 mM ammonium bicarbonate ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were digested on the column with 0.25 µg of Trypsin/Lys-C protease mix (Promega; cat. V5071), and peptides eluted for mass spectrometry.
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bioRxiv - Cell Biology 2021Quote: ... Halo-tagged proteins were labeled by incubating cells with 200nM HaloTag Ligand conjugated to Janelia Fluor 646 (Promega) for 30 min before imaging ...
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bioRxiv - Cell Biology 2021Quote: The Rosa26 promoter and the 5’UTRs of secretory granule protein transcripts were subcloned into the pNL1.1 (Promega) using standard cloning techniques ...