Labshake search
Citations for Promega :
1251 - 1300 of 1580 citations for GATOR2 complex protein WDR24 WDR24 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2023Quote: ... proteins were diluted with 50 mM NH4HCO3 to a final concentration of 2 M urea and digested with trypsin (Promega), at an enzyme-to-substrate ratio of 1:20 ...
-
bioRxiv - Microbiology 2023Quote: 1 ml of recombinant GST or GST-YgfB protein at a concentration of 10 μM was incubated with 100 μl 50 % MagneGST (Promega) bead-slurry equilibrated with pulldown-buffer for 45 min at 4°C and washed two times with 500 μl pulldown-buffer ...
-
bioRxiv - Immunology 2023Quote: ... Protein expression in luciferase-based assays was verified by analyzing the corresponding lysates stored in 1x passive lysis buffer (Promega) by Tricine-SDS-PAGE ...
-
bioRxiv - Plant Biology 2023Quote: ... Transcription factors were expressed using at least 2000 ng PCR product per sample with the TnT T7 Quick for PCR DNA in vitro protein expression kit (Promega). All reaction volumes were doubled to yield a total of 100 µL protein product per transcription factor ...
-
bioRxiv - Genetics 2023Quote: ... The HALO-NAC61 and GST-HALO (used as negative control) fusion proteins were translated in vitro using the TNTR SP6 coupled reticulocyte lysate system (Promega). Two replicates were used for the TF and input libraries ...
-
bioRxiv - Developmental Biology 2024Quote: Substrate titration kinase reactions were performed on the indicated recombinant LIN-42 proteins (CK1BD + Tail WT or mutants) using the ADP-Glo kinase assay kit (Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2024Quote: ... The expression of the NLuc-fusion proteins was initiated by washing out Dox and changing the medium into CDMi containing protected furimazine (Promega). After the NLuc signal was confirmed 4-5 hours later ...
-
bioRxiv - Plant Biology 2024Quote: ... and 1ug of plasmid DNA was used for in vitro protein expression using the TNT rabbit reticulocyte expression system (Promega) per the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2024Quote: ... 50 μL sample containing 50 μg proteins were mixed with 150 μL of rapid digestion buffer (Promega, Madison, Wisconsin, USA). Rapid trypsin (Promega ...
-
bioRxiv - Neuroscience 2024Quote: The HaloTag-fused proteins expressed in neurons (10 DIV) placed on coverslips were fluorescently labeled with 1 µM AF660 (Promega) in medium for 15 min at 37°C ...
-
bioRxiv - Microbiology 2024Quote: Enzymatic deglycosylation of denatured protein samples was performed with PNGase F (1 Unit/μL) obtained from Promega (Madison, WI, USA). First ...
-
bioRxiv - Immunology 2024Quote: ... or 0.05 μg proteins expression plasmids or empty pAc5.1A plasmids (as controls) using the Fugene HD Transfection Reagent (Promega Shanghai, China) according to the user manual ...
-
bioRxiv - Molecular Biology 2024Quote: Halo-tagged fusion proteins (200 pmol) were bound to 10 μL of Magne® HaloTag® Beads (20% slurry, Promega), followed by washing ...
-
bioRxiv - Biochemistry 2024Quote: ... Protein cleavage was initiated by the addition 5mM DTT (final concentration) and varying concentrations of ArgC protease (Promega, sequencing grade) with incubation at 37 °C for 2 hours ...
-
bioRxiv - Biochemistry 2024Quote: ... samples transferred to a fresh plate containing an equal volume of CETSA detection reagent – assay buffer supplemented with 100 nM of purified LgBiT-His protein and 0.5X furimazine (Nano-Glo® luciferase assay system, Promega). Samples were then incubated at room temperature for 30 minutes before transfer to a 384-well white plate (Greiner-One ...
-
bioRxiv - Biophysics 2024Quote: ... Proteins were digested to peptides by adding 25 µL of 50 mM ammonium bicarbonate containing 5 µg of trypsin (Promega) and incubating at 37 °C for 18 hours ...
-
bioRxiv - Plant Biology 2024Quote: ... Template for ribosome proteins CDS was the cDNA of Arabidopsis thaliana inflorescences obtained using ImProm-IITM Reverse Transcription System (Promega) from RNA isolated from plant tissue using RNeasy plant Mini Kit (Qiagen) ...
-
bioRxiv - Neuroscience 2024Quote: ... the procedure was the same except that protein extract samples were digested with Lyc-C (Lysyl Endopeptidase, WAKO) for 4 h and trypsin (Promega) overnight ...
-
bioRxiv - Molecular Biology 2024Quote: ... cells in each well were resuspended in 30 μL of TLR7-Fc or other Fc-tagged proteins (final concentration 5 μg/mL) in 0.5% BSA in 1x DPBS containing 200U/mL RNasein (Promega, N2511), and incubated on ice for 45 minutes ...
-
bioRxiv - Cancer Biology 2024Quote: ... Phoenix-AMPHO packaging cells were transfected with retroviral pBABE-puro-gateway constructs encoding ERBB4 variants or enhanced green fluorescent protein (eGFP) (vector control) using FuGENE 6 transfection reagent (Promega) according to manufacturer’s protocol ...
-
bioRxiv - Systems Biology 2024Quote: ... On-bead protein digestion was performed overnight in 50 mM triethylammonium bicarbonate (TEAB) pH 9 with mass spectrometry-grade trypsin (Promega) and LysC (FUJIFILM ...
-
bioRxiv - Biochemistry 2024Quote: The in vitro LLPS of all proteins was performed either in presence of varying concentrations of PEG-8000 (v/v) (Promega) or without any crowding agent in a dilution buffer composed of 50mM Tris pH 8 ...
-
bioRxiv - Biochemistry 2024Quote: ... S-trap columns containing the bound sample proteins were digested with sequencing grade modified trypsin (Promega, Fitchburg, WI, Cat # V5111). Upon completion of digestion ...
-
bioRxiv - Cell Biology 2024Quote: ... The denatured proteins were stabilized by washing twice with 50 mM ammonium bicarbonate and subjected to trypsin digestion (Promega, V115C) by incubating at 37C overnight ...
-
bioRxiv - Cell Biology 2024Quote: ... pH 8.5 to a final urea concentration of 2 M for Trypsin/Lys-C based overnight protein digestion at 37 °C (0.5 µg protease, Mass Spectrometry grade, Promega V5072). Peptide Purification and Labeling ...
-
bioRxiv - Cell Biology 2024Quote: ... followed by a 4-fold dilution with 50 mM HEPES buffer pH 8.5 and overnight trypsin digestion (50:1 protein : enzyme by weight, Promega V5280) at room temperature ...
-
bioRxiv - Cell Biology 2024Quote: ... protein samples were then incubated with chymotrypsin at a ratio of 1:80 (enzyme to protein) for 3-4 h at RT and then trypsin (Promega) at a ratio of 1:80 (enzyme to protein ...
-
bioRxiv - Cell Biology 2020Quote: ... We used the following primary antibodies: mouse anti-β-galactosidase (Promega, 1:1,000 dilution), rabbit anti-GFP (MBL,1:1,000 dilution) ...
-
bioRxiv - Cell Biology 2020Quote: ... The peroxidase-conjugated secondary antibodies were goat anti-mouse IgG (715-035-151, Promega) and anti-rabbit IgG (711-035-152 ...
-
bioRxiv - Cancer Biology 2022Quote: ... After 1h of incubation with a horseradish peroxidase-conjugated secondary antibody (1:3,000, Promega), protein bands were visualized using an enhanced chemiluminescence detection kit (Millipore ...
-
bioRxiv - Molecular Biology 2021Quote: ... Control immunoprecipitations (mock) were performed with a non-specific antibody (Anti-Rabbit IgG, Promega). Western blot analyses were performed using the following antibodies ...
-
bioRxiv - Biochemistry 2020Quote: ... and goat anti-rabbit IgG (H+L) antibody conjugated to HRP (Promega, catalog # W4018). Immunoblots were imaged using iBright 1500 (ThermoFisher ...
-
bioRxiv - Neuroscience 2021Quote: ... USA) at 1:10 dilution and polyclonal HaloTag antibody (Promega Corp. Madison, WI, USA) at 1:500 dilution and FITC-conjugated donkey anti-mouse secondary antibody and Cy3-conjugated goat anti-rabbit secondary antibody (Invitrogen).
-
bioRxiv - Cell Biology 2021Quote: ... HRP conjugated anti-rabbit (W401B) and anti-mouse (W402B) secondary antibodies were from Promega. CB-5083 was a gift from Cleave Biosciences and Bortezomib was from Selleckchem ...
-
bioRxiv - Microbiology 2021Quote: ... The secondary antibody is anti-guinea pig IgG conjugated to horseradish peroxidase (HRP) (Promega). ImageQuant LAS4000 camera (GE Healthcare Life Sciences ...
-
bioRxiv - Microbiology 2021Quote: ... Anti-mouse and anti-rabbit HRP-conjugated secondary antibodies were purchased from Promega (USA). Alexa Fluor 488 and Alexa Fluor 594 antibodies were purchased from Invitrogen (USA) ...
-
bioRxiv - Neuroscience 2020Quote: ... overnight at 4°C and HRP-conjugated anti-rabbit antibody (1:2,000, W4011, Promega) for 1 hr at room temperature followed by ECL signal amplification and chemiluminescence detection (SuperSignal West Pico Chemiluminescent Substrate ...
-
bioRxiv - Bioengineering 2022Quote: ... Horseradish peroxidase-conjugated anti-rabbit IgG (H + L) secondary antibody (W401B; Promega, Madison, WI) was used at a dilution rate of 1:5,000 for anti-TNF-α or -IL- 1β blotting ...
-
bioRxiv - Cancer Biology 2022Quote: ... Anti-mouse (W4028) and anti-rabbit (W4018) HPR-conjugated secondary antibodies were from Promega. Anti-Mouse Alexa Fluor 488 (A32790) ...
-
bioRxiv - Cancer Biology 2022Quote: ... Anti-mouse (W4028) and anti-rabbit (W4018) HRP conjugated secondary antibodies were from Promega. Anti-mouse Alexa® Fluor 488 (#A32790 ...
-
bioRxiv - Pathology 2021Quote: ... and anti-rabbit IgG antibody coupled to horseradish peroxidase (Promega France, W401B, 1/2500) and revealed with the Western Lightning chemiluminescence reagent Plus (PerkinElmer Life Sciences) ...
-
bioRxiv - Plant Biology 2021Quote: ... Expression of MYB24 was confirmed by western blot using an anti-HaloTag antibody (Promega). The protein expression reaction was mixed with HaloTag-ligand conjugated magnetic beads (Promega ...
-
bioRxiv - Immunology 2021Quote: ... adding rabbit anti-chicken IgY which HRP-conjugated secondary antibodies (Promega, Madison, WI, USA) in the dilution of 1:5000 and incubated at 37°C for an hour ...
-
bioRxiv - Microbiology 2023Quote: ... Immunoreactive bands were detected with alkaline phosphatase conjugated secondary antibodies and NBT/BCIP (Promega). Blots shown correspond to representative results of at least 2 independent experiments ...
-
bioRxiv - Neuroscience 2023Quote: ... Secondary antibodies used for western blot include anti-Rabbit-HRP (1:20000, Promega W4011); anti-Mouse-HRP (1:20000 ...
-
bioRxiv - Biochemistry 2023Quote: ... followed by the secondary anti-rabbit HRP-conjugated antibody (W401B, Promega, Madison, Wi, USA), diluted 1:10.000 in 5% BSA TBS-T ...
-
bioRxiv - Cancer Biology 2023Quote: ... followed by overnight incubation with primary antibodies rabbit anti-HaloTag (1:500, G9281, Promega) and mouse anti-LaminB1 (0.5ug/ml ...
-
bioRxiv - Immunology 2024Quote: ... Other antibodies used were anti-rabbit IgG-HRP conjugate (cat# W4011, Promega, Madison, WI), anti-mouse IgG-HRP conjugate (cat# W4021 ...
-
bioRxiv - Molecular Biology 2024Quote: ... Secondary antibodies used: anti-rabbit IgG (H+L) HRP conjugate (Promega W4011, 1:2,500); anti-mouse IgG (H+L ...
-
bioRxiv - Plant Biology 2020Quote: ... Proteins were dissolved in 40 mM ammonium bicarbonate and digested by 1 ug sequence-grade modified trypsin (Promega, Madison, WI, USA) at 37 °C overnight ...