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Citations for Promega :
801 - 850 of 1580 citations for GATOR2 complex protein WDR24 WDR24 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... for determination of viral RNA synthesis/protein expression or 50 µl CellTiter-Glo reagent (Promega) for determination of cell viability/ATP concentration ...
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bioRxiv - Plant Biology 2024Quote: ... Protein samples eluted with biotin were digested in-solution with 1 ug Trypsin/LysC (Promega) overnight at 37°C ...
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bioRxiv - Microbiology 2023Quote: Reduced and alkylated proteins were in-gel digested with 100ng trypsin (modified sequencing grade, Promega) overnight at 37° C ...
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bioRxiv - Plant Biology 2023Quote: ... The proteins were resuspended in 100 mM NH4HCO3 and were digested with trypsin (Promega, UK) at an enzyme-to-substrate ratio of 1:25 ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were produced in vitro via the TNT SP6 Quick Coupled Transcription/Translation System (Promega) following manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were extracted by methanol-chloroform precipitation and digested with 1 μg of trypsin (Promega) in 100 mM EPPS (pH 8.0 ...
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bioRxiv - Genomics 2023Quote: Proteins were expressed in rabbit reticulocyte lysate (TNT T7 Quick Coupled Transcription/-Translation System, Promega) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... 2.25 ng/uL Single-Stranded DNA Binding Protein (Sigma, cat# S3917 OR Promega, cat# M301A). 12.5 uL of master mix was mixed with 12.5 uL of 2x input oligonucleotide ...
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bioRxiv - Microbiology 2023Quote: ... followed by overnight digestion with trypsin (enzyme to protein ratio of 1:100) (Promega, Germany) at 37 °C ...
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bioRxiv - Molecular Biology 2023Quote: Proteins for CoIP were produced with the TnT Quick Coupled Transcription/Translation System (Promega, www.promega.com) according to the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were precipitated with trichloroacetic acid and digested with Lys-C (Wako) and trypsin (Promega). The peptides were then analysed using an Ultimate 3000 nano-RSLC (Thermo Scientific ...
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bioRxiv - Synthetic Biology 2023Quote: ... Bound protein was quantified using the Nano-Glo® HiBiT Extracellular Detection System (N2420, Promega). To quantify immobilized DNA in wells without protein ...
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bioRxiv - Cell Biology 2023Quote: ... Protein pellets were resuspended in the digestion buffer as previously described and trypsin (Promega, UK) digested to peptides74 ...
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bioRxiv - Genetics 2022Quote: Proteins were produced in vitro via the TNT SP6 Quick Coupled Transcription/Translation System (Promega). If two proteins were to be tested together in a single binding reaction ...
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bioRxiv - Cell Biology 2023Quote: ... and finally radiolabelled precursor proteins were synthesized with the Flexi rabbit reticulocyte lysate system (Promega). 35S-radiolabeled precursor proteins were incubated with isolated mitochondria in import buffer (3% (w/v ...
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bioRxiv - Biochemistry 2023Quote: Proteins labeled with [35S]-methionine were produced by coupled transcription and translation reactions (T7, Promega). Translation products were partially purified by ammonium sulfate precipitation [87] ...
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bioRxiv - Biochemistry 2023Quote: ... coli and HeLa cells as well as of a commercially available yeast protein digest (Promega), The ratios of the peptide amount of each species within the three mixtures are given in Table S1.
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bioRxiv - Neuroscience 2023Quote: ... Proteins were digested overnight at 37° C with 1 μg mass spectrometry grade Trypsin (Promega). The resulting peptide sample was acidified with 10% formic acid and 10% trifluoroacetic acid (TFA ...
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bioRxiv - Plant Biology 2023Quote: Proteins were expressed using the TnT Coupled Wheat Germ Extract System with SP6 promoter (Promega). Protein expression was confirmed by Western Blot ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were resuspended in 100 mM NH4HCO3 and were digested with trypsin (Promega, UK) at an enzyme-to-substrate ratio of 1:25 ...
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Arabidopsis actin-binding protein WLIM2A links PAMP-triggered immunity and cytoskeletal organizationbioRxiv - Plant Biology 2023Quote: ... Proteins were then digested overnight at 37°C with trypsin (Porcine trypsin, Promega, Fitchburg, WI). After stopping the digestion with 1% formic acid ...
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bioRxiv - Cell Biology 2023Quote: ... Test proteins were generated in vitro using the TNR quick-coupled transcription-translation kit (Promega). For radiolabeling ...
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bioRxiv - Neuroscience 2023Quote: ... proteins on beads were digested with the addition of 2 µg trypsin (Promega, Cat# V511A) for 18 h at 37°C.
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bioRxiv - Molecular Biology 2023Quote: ... we labeled HALO-tagged49 proteins with HALO-Alexa488 or HALO-TMR dyes (Promega, G1001, G8251) by incubating the concentrated protein with dye for 1 hour on ice ...
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bioRxiv - Biochemistry 2024Quote: ... For protein synthesis in reticulocyte lysate the TNT® Quick Coupled Transcription/Translation System (Promega) was used and performed according to the manufacture’s protocol employing 1 mM unlabeled methionine ...
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bioRxiv - Cell Biology 2024Quote: ... cells transfected with HALO-fusion protein were supplemented with 100 nM TMR HALO ligand (Promega). Cells were fixed at RT for 20 min in 3.7% formaldehyde (FA ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were digested by adding 5 μL of sequencing grade trypsin (0.1 mg/mL) (Promega) for three h at 37 °C ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were then digested using 250 ng of trypsin enzyme (Sequencing Grade Modified Trypsin, Promega) and incubation for 1h at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were digested with a solution of 0.05 μg/μL of trypsin/Lys-C (Promega) in 100 μL of AMBIC overnight at 37 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were subjected to overnight digestion with mass spec grade Trypsin/Lys-C mix (Promega). Following digestion ...
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bioRxiv - Neuroscience 2024Quote: ... the protein-containing gel piece was excised and digested with trypsin/Lys-C mix (Promega). Peptides were analyzed by a data-independent acquisition (SWATH ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins on the beads were digested by adding 200 ng trypsin/Lys-C mix (Promega) at 37 °C overnight ...
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bioRxiv - Microbiology 2024Quote: ... In-gel protein digestion was performed overnight at 37°C with sequencing grade trypsin (Promega) in a protease:protein ratio of 1:50 (w/w) ...
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bioRxiv - Biophysics 2024Quote: The McsB-HaloTag protein was labeled with the Janelia Fluor® HaloTag® Ligands (Promega) following the manufacture’s procedures ...
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bioRxiv - Biochemistry 2024Quote: Endogenous BRD4 protein levels were evaluated using the Nano-Glo HiBiT Lytic Detection System (Promega). In brief ...
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bioRxiv - Biochemistry 2024Quote: ... for expression in the TnT® SP6 High-Yield Wheat Germ Protein Expression System (Promega). Using Gibson Assembly ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were digested at 37°C overnight with 0.7 μg Trypsin/Lys-C mix (Promega). Afterwards ...
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bioRxiv - Cell Biology 2024Quote: ... Cells expressing HaloTag-fusion proteins were supplemented with 100 nM tetramethylrhodamine (TMR) HaloTag ligand (Promega). After treatment ...
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bioRxiv - Biochemistry 2024Quote: ... and the proteins were digested overnight at 37°C using 100:1 protein:trypsin ratio (Promega). After digestion ...
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bioRxiv - Plant Biology 2022Quote: ... as primary antibodies and anti-mousse IgG-HRP conjugated secondaries antibodies (Mouse IgG, HRP conjugate W402B, Promega; 1/5000 in TBST, 4 hours). As a loading control we used anti-tubulinα antibodies as primary antibodies (1/1000 in 5 % milk over-night) ...
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bioRxiv - Biochemistry 2020Quote: ... and Alexa Fluor 680 goat anti-mouse antibody (Promega, W402B). Blots were imaged on a Peqlab Fusion SL Vilber Lourmat ...
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bioRxiv - Biochemistry 2020Quote: ... anti-rabbit AP-conjugated antibody was from Promega (Southampton, UK); Alexa 488-conjugated anti-mouse IgG and Alexa568-conjugated phalloidin were from Life Technologies (Paisley ...
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bioRxiv - Cell Biology 2021Quote: ... HRP-conjugated anti-rabbit and anti-mouse secondary antibodies (Promega) were used at dilution 1:10.000 in PBS-T supplemented with 5% instant nonfat dry milk for 1 hour at RT ...
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bioRxiv - Developmental Biology 2021Quote: ... and HRP-labelled secondary antibodies (RRID:AB_430833; RRID:AB_430834; Promega; 1:2,000) were used ...
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bioRxiv - Cancer Biology 2020Quote: ... and incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies (Promega) at room temperature for 1 hour ...
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bioRxiv - Cell Biology 2021Quote: ... Secondary antibodies conjugated to horseradish peroxidase were purchased from Promega, GE Healthcare ...
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bioRxiv - Cell Biology 2021Quote: ... Appropriate secondary horseradish peroxidase conjugated antibodies (Promega, Madison, WI, USA) were applied ...
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bioRxiv - Microbiology 2022Quote: ... As secondary antibodies α-mouse IgG HRP conjugate (Promega W4021) or α-rabbit IgG HRP conjugate (Cell Signaling #7074 ...
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bioRxiv - Molecular Biology 2021Quote: ... Appropriate HRP-conjugated secondary antibodies were used (1:10000 Promega). Chemiluminescent signals were detected and digitally analyzed ...
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bioRxiv - Genetics 2020Quote: ... and incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies (Promega) at room temperature for 1 hr ...