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Citations for Promega :
1051 - 1100 of 1580 citations for GATOR2 complex protein WDR24 WDR24 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2023Quote: ... Proteins were digested off the beads by adding 6 μL of 1 μg/μL sequencing grade Trypsin (Promega), diluted in 50 mM acetic acid ...
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bioRxiv - Cell Biology 2023Quote: 35S-radiolabeled Tom40 precursor proteins were synthesized in vitro using the TNT Quick Coupled Transcription/Translation kit (Promega). Transcripts for MCO6 encoding 3 additional N-terminal methionine residues for radiolabelling of the Mco6 protein which lacks internal methionine residues ...
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bioRxiv - Cell Biology 2023Quote: ... the gel plugs were digested by using 1:50 (Trypsin: Protein amount ratio) Sequencing Grade Modified Trypsin (Promega) at 37 °C overnight ...
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bioRxiv - Molecular Biology 2023Quote: Reduced and alkylated proteins were prepared by in-gel digesting overnight at 37°C using 100ng trypsin (Promega). The supernatant was dried by vacuum centrifugation and the samples were resuspended in 0.1% trifluoro acetic acid ...
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bioRxiv - Molecular Biology 2023Quote: ... proteins were directly digested by incubation with sequencing-grade modified trypsin (1/50, w/w; Promega, Madison, Wisconsin) overnight at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were digested in 100 mM ammonium bicarbonate containing 2 μg of trypsin/Lys-C enzyme mix (Promega) at 37 °C overnight ...
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bioRxiv - Systems Biology 2023Quote: ... Samples were then digested by a 1:50 (trypsin to protein) ratio of sequencing grade modified trypsin (Promega) for 16 hours at 600 rpm and 25°C ...
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bioRxiv - Biochemistry 2024Quote: ... 30 µg of proteins from lysed cells were digested with 500 units of endoglycosidase H (ENDO H, Promega) and 10 units of Peptide N-glycosidase F (PNGase F ...
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bioRxiv - Plant Biology 2024Quote: ... flag-tagged MED25 protein and CFP-tagged RAP2.2 and RAP2.12 proteins (Schmidt et al., 2018) were expressed using TNT SP6 High Yield Wheat Germ (Promega) following the manufacturer’s instructions ...
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ALiCE®: A versatile, high yielding and scalable eukaryotic cell-free protein synthesis (CFPS) systembioRxiv - Biochemistry 2022Quote: ... purified protein samples were prepared by S-alkylation with iodoacetamide and digestion with either with LysC/GluC (Promega) [RBD] or ...
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bioRxiv - Biochemistry 2022Quote: ... laevis TRAIP that was produced in the TnT® SP6 High-Yield Wheat Germ Protein Expression System (Promega) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... The HaloTag-CpOGA proteins were mixed with equal molar of HaloTag Alexa Fluor 488 or 660 ligands (Promega) and incubated at room temperature for 30 min ...
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bioRxiv - Physiology 2022Quote: ... Luciferase activities were measured and plotted relative to lysate protein concentrations using the Promega Dual Luciferase Reporter (Promega) assays according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... Digestion reaction for proteins was initially set up with 1 μg of MS grade LysC (Promega, Cat# PI90307) for 3 hours at RT and then continued overnight with addition of 2 μg of trypsin (Promega ...
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bioRxiv - Plant Biology 2023Quote: ... 1 μg protein was used for dual luciferase transactivation assay using Dual-Luciferase® Reporter Assay System (Promega) following manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... immunodepleted protein samples were successively digested with LysC (0.008 μg/μL, Wako) and trypsin (0.002 μg/μL, Gold, Promega), using a filter-aided sample preparation (FASP ...
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bioRxiv - Plant Biology 2023Quote: ... In-gel digestion of proteins was performed at 37 °C overnight using 2.5 μg Trypsin/Lys-C (Promega). Samples were further digested with 2.5 μg Chymotrypsin (Promega ...
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bioRxiv - Plant Biology 2023Quote: ... LUC activity was measured with 20 mL of protein sample and 100 mL of LUC assay reagent (Promega). Promoter activation was calculated as (rate of MUG accumulation / LUC luminescence ...
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bioRxiv - Plant Biology 2024Quote: ... LUC activity was measured with 20 μl of protein sample and 100 μl of LUC assay reagent (Promega) using Clariostar plate reader ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were then digested into peptides by the addition of 1:1000 (g/g) Lys-C Protease (Promega) for 4 h at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... Concentrated protein extracts were then digested with 3 µl trypsin according to manufacturer’s instruction (Promega, Madison, WI, USA) at 37 °C overnight ...
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bioRxiv - Cell Biology 2024Quote: ... precipitated proteins were resuspended in 0.1% RapiGest with 6 μL of sequencing-grade trypsin (Promega, 0.5 μg/μL) added to each sample and digested overnight at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... The proteins were diluted to 2 M urea with Tris-HCl and digested with sequencing-grade trypsin (Promega) at an enzyme:protein ratio of 1:25 for 12 hr at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... The single protein band was visualized by colloidal Coomassie Blue staining and in-gel digested with Trypsin (Promega). Peptides were extracted with 0.1% trifluoroacetic acid in 65% acetonitrile and dried in a speedvac ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were digested with 1:50 w/w LysC (Wako Chemicals) and 1:100 w/w trypsin (Promega) overnight at 37°C after dilution to a final urea concentration of 1M using 50mM Tris pH 8.5 ...
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bioRxiv - Neuroscience 2024Quote: ... excised from the gel and protein enzymatic cleavage was carried out with trypsin (Promega; 1:20, w/w) at 37 °C for 16 h as previously described (Shevchenko et al. ...
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bioRxiv - Biochemistry 2024Quote: Endogenous BRD4 protein levels were evaluated using the Nano-Glo HiBiT Lytic Detection System (Promega, Madison, WI, USA). Briefly ...
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bioRxiv - Cell Biology 2020Quote: ... As secondary antibodies we used horseradish peroxidase coupled anti-mouse (Promega W402B), anti-rabbit (Promega ...
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bioRxiv - Microbiology 2020Quote: ... Immune complexes were detected with anti-rabbit peroxidase-conjugated secondary antibodies (Promega) and enhanced chemiluminescence reagents (Pierce ...
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bioRxiv - Developmental Biology 2021Quote: ... The primary antibodies used are mouse β-Gal (1:250; Promega #Z3781), mouse anti-Wingless (1:100 ...
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bioRxiv - Molecular Biology 2021Quote: ... 200 μL of rabbit polyclonal antibody against PLRV-CP (1:5000; Promega) was added to each well and incubated for 3 h at 37 °C ...
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bioRxiv - Microbiology 2021Quote: ... Secondary antibodies used in Western blotting were conjugated to alkaline phosphatase (Promega) or horseradish peroxidase.
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bioRxiv - Microbiology 2020Quote: ... Secondary HRP conjugated goat anti-mouse IgG antibodies (Promega, Madison, WI; W4021) was used at 1:50,000.
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bioRxiv - Cell Biology 2022Quote: ... Secondary antibodies conjugated to horseradish peroxidase (1:10,000, W401B and W402B, Promega) were applied in the same solution for one hour at room temperature before activity was detected (SuperSignal West Pico PLUS 34580 ...
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bioRxiv - Molecular Biology 2020Quote: ... and the goat anti-rabbit antibody (anti-rabbit IgG AP conjugate, Promega) as secondary antibody (30) ...
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bioRxiv - Genetics 2021Quote: ... the following antibodies were used: mouse anti-beta-galactosidase (Promega; 1:100), mouse anti-Mmp1 (1:1:1 mixture of DSHB 3A6B4 ...
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bioRxiv - Microbiology 2023Quote: ... Secondary antibodies used in Western blotting were conjugated to alkaline phosphatase (Promega) or horseradish peroxidase.
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bioRxiv - Microbiology 2023Quote: ... followed by horseradish peroxidase-conjugated (HRP) secondary rabbit antibodies (Promega, 1/25000) and revealed by chemiluminescence (Pierce ...
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bioRxiv - Developmental Biology 2024Quote: ... the membrane was incubated in secondary antibody conjugated to horseradish peroxidase (Promega) at a dilution of 1:5000 for 1h at room temperature ...
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bioRxiv - Genetics 2023Quote: ... Secondary horseradish peroxidase-conjugated anti-mouse or anti-rabbit IgG antibodies (Promega) were used at 1 ...
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bioRxiv - Neuroscience 2023Quote: ... The immunoblots were probed with appropriate secondary antibodies (Promega and Li-Cor) and visualised by chemiluminescence (Supersignal detection kit ...
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bioRxiv - Biochemistry 2023Quote: ... Secondary antibodies used were: anti-rabbit-IgG-HRP (W401B, 1:8000, Promega); anti-mouse-IgG-HRP (W402B ...
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bioRxiv - Biochemistry 2022Quote: ... membrane was incubated with anti-HaloTag antibody (1:1000; Promega Corporation; G9281) and then with anti-Rabbit IgG Horseradish Peroxidase linked whole antibody (1:5000 ...
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bioRxiv - Cancer Biology 2023Quote: ... Antibody signal was detected using the ECL Western Blotting Substrate (W1015, Promega) and X-ray film (F-BX810 ...
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bioRxiv - Molecular Biology 2024Quote: ... secondary antibodies: anti-rabbit HRP or anti-mouse HRP (Promega, 1:10000).
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bioRxiv - Genomics 2024Quote: ... The following secondary antibodies were used: anti-rabbit IgG HRP (Promega, #W4018). Blots were incubated with the primary antibody overnight at 4 °C ...
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bioRxiv - Neuroscience 2024Quote: ... Bound antigen-antibody complexes were precipitated using streptavidin-coated magnetic beads (Promega) for at least 1 hr at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were digested with LysC (Wako) (substrate:enzyme=100) overnight at 37°C and then with sequencing grade trypsin (Promega) (substrate:enzyme=100 ...
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bioRxiv - Cell Biology 2020Quote: ... securin-CFP was in vitro translated using the TNT SP6 high-yield wheat germ protein expression system (Promega, #L3261). Three or 4 µL of in vitro translated securin-CFP was added to 50 or 80 µL extract ...
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bioRxiv - Immunology 2021Quote: ... After 8 days of transfection mAbs were purified from culture supernatant using Magna Protein A beads (Promega, WI, USA).