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Citations for Promega :
851 - 900 of 1323 citations for Rh Family C Glycoprotein RHCG Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2022Quote: ... Denatured proteins were then enzymatically digested into peptides upon incubation first with endoproteinase LysC (Wako Laboratories) at 25 °C shaking for 2 h and then with sequencing-grade trypsin (Promega) at 25 °C shaking overnight ...
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bioRxiv - Microbiology 2022Quote: ... The cells were incubated for 24 hrs at 37 °C (for HEK-293T) or 39 °C (DF-1) and luciferase activity was measured by using the Dual-Glo luciferase assay system (Promega). The polymerase activity was calculated by normalising firefly luciferase activity relative to the Renilla luciferase activity.
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bioRxiv - Microbiology 2022Quote: ... at 72° C for 10 min to add 3’-A overhangs for T/A cloning with pGEM-T Easy plasmid (Promega). A 610 bp DNA fragment upstream of flgV was amplified using PCR primers 85 and 86 ...
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bioRxiv - Microbiology 2022Quote: Salmonella strains were cultured overnight at 37°C in LB broth and DNA was extracted using a Maxwell RSC 48 instrument (Promega) and associated Maxwell RSC cultured cells DNA kit (Promega) ...
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bioRxiv - Plant Biology 2022Quote: ... were reduced with DTT and then alkylated with iodoacetamide and digested overnight using Lys-C/Trypsin (1:50, enzyme to protein; Promega). After terminating the digestion with 1% trifluoroacetic acid ...
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bioRxiv - Molecular Biology 2023Quote: ... Oligos were then ligated at 4°C overnight to the appropriate digested ACR-16-REM sequence (T4 ligase, Promega, USA) and transformed into DH5α (ThermoScientific ...
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bioRxiv - Microbiology 2023Quote: ... The plate was incubated for 48 hours at 37°C in a CO2 incubator and luciferase activity was quantified by adding nano-glo luciferase substrate (Promega). The luminescence was recorded by using Cytation 5 plate reader (BioTek inc) ...
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bioRxiv - Molecular Biology 2022Quote: ... the Immunoprecipitated samples were in-solution digested overnight at 37 °C in 400 ng of mass spectrometry grade trypsin (Promega) enzyme ...
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bioRxiv - Molecular Biology 2022Quote: ... expressing PALLD and pNLF1-N [CMV Hygro] or pNLF1-C [CMV Hygro] vector (Promaga) expressing ANKRD1 were treated with 100 nM HaloTag NanoBRET 618 Ligand (Promega), whereafter signals were detected six hours later using a Synergy 4 instrument (BioTek) ...
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bioRxiv - Plant Biology 2022Quote: ... Hybridization probes were generated by PCR amplifi cation using gene specifi c primers (Table S1) and radiolabeled with the Prime A Gene labeling kit (Promega) following the recommendations of the manufacturer.
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bioRxiv - Systems Biology 2022Quote: ... and digested with 3 μg LysC (Wako) O/N at 37°C and then with 3 μg of trypsin (Promega) for eight hours at 37°C following FASP procedure (Filter-aided sample preparation 48) ...
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bioRxiv - Cell Biology 2024Quote: ... cells were incubated for 1 h at 37 °C with fresh complete DMEM containing JFX646 HaloTag ligand (200 nM; GA112A; Promega), washed twice with PBS 1X and imaged on an Elyra 7 with Lattice SIM² microscope (Zeiss ...
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bioRxiv - Cell Biology 2024Quote: ... Digestion was performed over-night at 37°C by addition of 20 µL of trypsin (Sequencing Grade Modified Trypsin, Promega) at 37.5 ng/µL in 50 mM ammonium bicarbonate ...
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bioRxiv - Cell Biology 2024Quote: ... the samples were diluted with seven volumes of 50 mM ammonium bicarbonate and incubated for 16 h at 37 °C with 400 ng of trypsin (ratio 1:50; “Trypsin Gold”, Promega).
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bioRxiv - Molecular Biology 2024Quote: ... 40 µL of digestion solution (1.25 mM TCEP, 5 mM chloroacetamide, 0.2 µg trypsin/Lys-C mix (Promega, cat#V5073), in 100 mM HEPES pH 8.5 ...
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bioRxiv - Microbiology 2024Quote: ... cells were infected at MOI 1 for 24 h and cells were frozen dry at -80°C for 30 min and lysed in Passive Lysis Buffer (Promega). Bioluminescence was measured using the Nano-Glo® Luciferase Assay System (Promega ...
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bioRxiv - Cancer Biology 2024Quote: ... Reactions were incubated for 1.5 hr at 30 °C and luciferase signal was measured using the Dual-Glo Luciferase Assay System (Promega E2920).
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bioRxiv - Microbiology 2024Quote: ... In vitro mRNA synthesis was then carried out at 37 °C for 4 h in T7 RiboMAX Large Scale RNA Production System kit (Promega) according to the manufacturer’s recommendations ...
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Parkinson’s VPS35[D620N] mutation induces LRRK2 mediated lysosomal association of RILPL1 and TMEM55BbioRxiv - Neuroscience 2023Quote: ... On-column digestion was performed by incubating 60 µL (1.5 µg) Trypsin/Lys-C Mix (Mass Spec Grade; Promega, UK) in 50 mM TEABC solution at pH 8 on the samples on a thermomixer at 47°C for 1 hour before reducing the incubation temperature to 22°C for overnight digestion ...
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bioRxiv - Immunology 2024Quote: ... the samples were quenched with 10 mM DTT and digested overnight at 37°C with Trypsin gold (Promega, WI, USA). The digestion was stopped and the surfactant was cleaved by treating samples with 200 mM HCl (Sigma ...
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bioRxiv - Physiology 2024Quote: ... Cysteine residues were alkylated in presence of 20 mM iodoacetamide at 37 °C in the dark for 30 min and tryptic digestion (sequencing grade, Promega) was performed at a 100:1 protein to enzyme ration at 37 °C overnight ...
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bioRxiv - Microbiology 2023Quote: ... The inoculum was added to HEK293T cells and incubated at 37 ° C with 5% CO2 and the luciferase signal was with analyzed using luciferase assay kit (Promega).
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bioRxiv - Cell Biology 2023Quote: ... A 250 ug aliquot was alkylated and digested by incubating overnight at 37 °C in trypsin at a ratio of 1:50 (Promega). Digestion was acidified by adding formic acid (FA ...
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bioRxiv - Cancer Biology 2023Quote: ... Alkylated proteins were then cleaved using a two-step digestion: first with Endoproteinase Lys-C (ratio 1:33 enzyme: lysate, Promega) for 1 hour at 37 °C then with Trypsin (ratio 1:33 enzyme ...
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bioRxiv - Biochemistry 2024Quote: ... at a 1:75 enzyme to protein ratio for 6 h at 30 °C and then by trypsin (V5111, Promega) (1:50 enzyme to protein ratio ...
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bioRxiv - Biochemistry 2024Quote: ... The binding of the Nbs to the coated protein was detected via their EPEA-tag using a 1:4000 CaptureSelectTM Biotin anti-C-tag conjugate (Thermo Fischer Scientific) in combination with 1:1000 Streptavidin Alkaline Phosphatase (Promega). Colour was developed by adding 100 µL of 3 mg/mL disodium 4-nitrophenyl phosphate solution (DNPP ...
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bioRxiv - Cancer Biology 2024Quote: ... The solution was then diluted to 4 mL with 50 mM ammonium bicarbonate and subjected to overnight digestion at 37°C with trypsin (Promega) using an enzyme to substrate ratio of 1:200 (w/w) ...
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bioRxiv - Cancer Biology 2024Quote: ... Denatured proteins were enzymatically digested for 14 h at 37 °C with 1 µg of sequencing-grade Trypsin (V511A, Promega). After speed-vaccum drying ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was digested overnight at 37 °C with sequencing-grade trypsin or chymotrypsin (Promega; 1:100 protease:protein weight ratio). After desalting on a C18 column (Pierce ...
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bioRxiv - Molecular Biology 2023Quote: ... The mixture was incubated at 25°C for 30 minutes and the production of ADP was measured using the ADP-Glo kinase assay kit (Promega). Vmax was calculated after fitting the kinase activity data to the Michaelis–Menten equation ...
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bioRxiv - Microbiology 2022Quote: ... The plates were incubated at 37°C for ~12 hours before luciferase activity was quantified using the Luciferase Assay System (Promega) using SoftMax Pro v.7.0.2 (Molecular Devices) ...
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bioRxiv - Microbiology 2022Quote: ... and alkylated with 5 mM of iodoacetamide 30 min in the dark at RT°C prior to digestion with 5 μg of trypsin Gold (Promega) for 15 h at 37 °C ...
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bioRxiv - Molecular Biology 2023Quote: Protein samples were treated with Endoprotease Lys-C (Fujifilm Wako chemicals, Osaka, Japan) and Trypsin (Trypsin Gold Mass Spec Grade, Promega). Peptide samples were desalted using OMIX C18 pipette tips (Agilent Technologies) ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were subjected to in gel tryptic digest overnight at 37 °C with approximately 2 µg trypsin per 100 µl gel volume (Trypsin Gold, mass spectrometry grade, Promega). Peptides were eluted twice with 100 mM NH4HCO3 followed by an additional extraction with 50 mM NH4HCO3 in 50% acetonitrile ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the coding sequence of HIV-1 Nef (SF2 variant) was amplified by PCR and inserted into the plasmid pNLF-1-C (Promega) for expression of NanoLuc at the Nef C-terminus ...
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bioRxiv - Developmental Biology 2023Quote: Protein extracted from 10 CNC explants as described above or immunoprecipitated with the Flag antibody from embryos or Hek293T cells were diluted in 8M urea and processed with Trypsin/Lysine-C according to manufacturer instruction (Promega). Tandem mass spectrometry was performed using a Thermo orbitrap Fusion (Mass spectral data were obtained at the University of Massachusetts Mass Spectrometry Core Facility ...
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bioRxiv - Biochemistry 2023Quote: ... stable HEK293T cells expressing wild type COMT were transiently transfected with pTagBFP-C expressing the KDEL marker using FugeneHD (Promega) following the manufacturer’s instructions with 100 ng plasmid ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with or without 50 μM adenosine 5′-triphosphate (ATP) at 30 °C for 30 minutes followed by kinase assays using ADP-Glo Kinase Assay kit (Promega). Data were analyzed using GraphPad Prism 9 ...
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bioRxiv - Developmental Biology 2023Quote: ... including the identified m6A site (wildtype sequence or A-to-C mutation) was cloned into the pmirGLO Dual-Luciferase miRNA Target Expression Vector (Promega), per manufacturer ’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Strap binding buffer was applied to precipitate proteins on quartz and proteolysis took place during 14 hrs at 37°C with 1 µg Trypsin sequencing grade (Promega). After speed-vacuum drying of eluted peptides ...
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bioRxiv - Cancer Biology 2023Quote: ... samples resuspended in 5%SDS buffer were reduced with DTT and alkylated, followed by digestion on a S-Trap column (ProtiFi, LLC)with sequencing-grade Lys-C/trypsin (Promega). The resulting peptides were were analyzed with a nanoAcquity UPLC system (Waters ...
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bioRxiv - Biochemistry 2023Quote: ... Translation reactions were incubated for 23 min at 30 °C and nanoluciferase activity was monitored using the Nano-Glo Luciferase Assay Kit (Promega) in a Microplate Luminometer (Veritas) ...
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bioRxiv - Systems Biology 2023Quote: ... 37.5 µL of 8 M Urea and digested overnight at room temperature with 1 µg/µL endoproteinase Lys-C (Wako) followed by digestion with sequencing-grade trypsin (Promega) at a final concentration of 1 ng/μL 6 h at 37 °C ...
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bioRxiv - Developmental Biology 2023Quote: ... Sample cleanup was performed using the SP3 method 83 and on-bead digestion was performed overnight using Trypsin/Lys-C (Promega). Eluted tryptic peptides from SP3 beads were pre-fractionated by high pH reverse phase chromatography into 96 individual fractions using a 64-minute gradient from 1% to 50% solvent B (A ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were incubated for 24hr at 37°C and cell death was measured through a lactate dehydrogenase (LDH) assay using the CytoTox 96 kit (Promega). 50 μL of media was collected from all experimental wells with two replicates each and placed in a fresh 96-well plate ...
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bioRxiv - Microbiology 2023Quote: ... The reactions were subjected to primer extension at 42°C for 45 min using 1 unit of MMLV-RT (Promega) and 0.5 mM of dNTPs ...
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bioRxiv - Biochemistry 2023Quote: ... Cell culture medium was replaced with pre-heated (37 °C) fresh medium containing EnduRen (60 µM) or 1x Nano-Glo Endurazine (Promega). Luciferase activity was measured using LuminoCell device ...
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bioRxiv - Microbiology 2023Quote: ... In solution digests were performed overnight at pH 8 and 37 °C with mass spectrometry grade trypsin (Promega, Madison, WI) at a 1:33 enzyme-to-protein ratio and quenched with formic acid (0.15%) ...
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bioRxiv - Biochemistry 2023Quote: ... N- glycan release was achieved after digestion with PNGase F (1 U/10 µg protein at 37 °C overnight; Promega). Released N-glycans were hydrolysed with 25 µl of 100 mM ammonium acetate at pH 5 (1 h at RT) ...
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bioRxiv - Developmental Biology 2023Quote: ... Wnt3A was added and incubated for another 6h at 37°C and luciferase activity was measured using the Dual-Luciferase Assay System (Promega). Each measurement was repeated with at least three independent transfections.