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Citations for Promega :
1001 - 1050 of 1323 citations for Rh Family C Glycoprotein RHCG Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... followed by incubation with their respective HRP-conjugated secondary antibodies (Promega). The membrane was soaked with Chemi-Lumi Super (Nakarai Tesque ...
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bioRxiv - Microbiology 2022Quote: ... and an anti-mouse IgG HRP conjugate secondary antibody (Promega, #W4021) (13) ...
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bioRxiv - Biophysics 2020Quote: ... The bead-antibody complexes were captured using a magnetic stand (Promega) and the suspensions were removed ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were then incubated with an HRP-conjugated secondary antibody (Promega) for one hour ...
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bioRxiv - Genetics 2020Quote: ... Alkaline phosphatase-conjugated secondary antibodies were used at 1:10000 (Promega). Western blot band intensity was analyzed with ImageJ Gel quantification tool.
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bioRxiv - Biochemistry 2021Quote: ... 11S protein was detected with a rabbit polyclonal antibody from Promega, and Tom40 with a rabbit polyclonal antibody produced by Cambridge Research Biochemicals (Billingham ...
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bioRxiv - Cell Biology 2021Quote: ... Primary antibodies used were anti-p75NTR (Promega, Cat: G323A, 1:300) and anti-GAPDH (Sigma ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by the anti-rabbit HRP-conjugated secondary antibody (#W4011; Promega).
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bioRxiv - Microbiology 2022Quote: ... and verified by Western blotting with the anti-HaloTag antibody (Promega). Genomic DNA was extracted from JP2-270 using DNAiso (Takara ...
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bioRxiv - Microbiology 2024Quote: ... Mouse antibodies (CV801, CV804, CV820, CV925, CV1117) and effector cells (Promega) expressing mouse FcγRIV and NFAT luciferase reporter were added to the cells ...
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bioRxiv - Molecular Biology 2024Quote: ... Rabbit anti-HaloTag® polyclonal antibody (G9281) was obtained from Promega. Mouse anti-tubulin monoclonal antibody (66031–1-Ig ...
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bioRxiv - Plant Biology 2024Quote: ... and HRP-conjugated secondary antibody (Promega, Cat# W4011, 1:4,000 dilution).
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bioRxiv - Immunology 2024Quote: Recombinant antibodies were purified using protein G magnetic beads (Promega #G7472), HiTrap protein G HP columns (Cytiva #17040401) ...
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bioRxiv - Cancer Biology 2023Quote: ... Membrane-bound antibodies were detected by the enhanced chemiluminescence system (Promega). Primary antibodies were rabbit anti-EGFR (Cell Signaling Technology #4267 ...
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bioRxiv - Cell Biology 2023Quote: ... The following antibodies were used: HaloTag mAB (Promega, G9211, 1:500), GAPDH (Cell Signaling 2118 ...
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bioRxiv - Biochemistry 2023Quote: ... The following primary antibodies were used: NanoLuc (N7000, Promega, 1:1000), β-ACTIN (#4970S ...
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bioRxiv - Molecular Biology 2023Quote: ... Mouse Anti-LgBiT monoclonal antibody was purchased from Promega (cat. #N7100), HRP-linked anti-mouse IgG was used as a secondary antibody and purchased from Cell Signaling Technology (7076S) ...
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bioRxiv - Immunology 2023Quote: ... All secondary HRP-conjugated antibodies were obtained from Promega (Madison, WI). For immunofluorescence only ...
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bioRxiv - Biochemistry 2024Quote: ... To resolve bands recognized by the phospho-p38 antibody (Promega #V1211), a NuPAGE™12% Bis-Tris SDS-PAGE gel (Invitrogen Fisher NP0349BOX ...
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bioRxiv - Biochemistry 2020Quote: ... The sample was then incubated at 37°C for overnight trypsin digestion (trypsin 1:50 protein w/w ratio; Promega, Madison, WI). The digestion reaction was quenched by adding formic acid (FA ...
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bioRxiv - Cell Biology 2020Quote: ... 1 μg of pCS2-HA-NFATc3 was incubated for 2 h at 30°C in 50 μl of the TNT® SP6 coupled wheat germ extract system (Promega, #L5030), according to the instructions of the manufacturer ...
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bioRxiv - Biochemistry 2020Quote: ... Trypsinisation was carried out by incubating the gel pieces overnight at 37 °C in 50 µl of trypsin (1: 25:: trypsin: protein, 20 µg/ml, sequencing grade trypsin V5111, Promega, Madison, WI) prepared in ammonium bicarbonate (50 mM) ...
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bioRxiv - Microbiology 2019Quote: ... cells were pelleted at 1000 rcf for 5 min at 4 °C and resuspended in luciferase reporter lysis buffer (Promega, Cat. #E4530) with 1% protease inhibitor cocktail (Millipore Sigma ...
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bioRxiv - Molecular Biology 2021Quote: ... protein ratio of 1:50 w/w for 2 hours at 37°C and further digested with 30 µL 50 mM TEAB containing trypsin (Sequencing Grade Modified, Promega, Madison, WI) at a trypsin ...
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bioRxiv - Immunology 2021Quote: ... and bound proteins were subjected to on-column digestion using mass spec grade Trypsin/Lys-C Rapid digestion enzyme (Promega, Madison, WI) at 70 °C for 2 h ...
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bioRxiv - Genomics 2019Quote: ... cells were incubated at 37°C with MTS for 90 min following the instruction of CellTiter 96 AQueous One Solution Reagent (Promega, Madison, USA). The absorbance was measured at the wavelength of 490 nm by a Synergy H1 plate reader (BioTek ...
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bioRxiv - Cell Biology 2019Quote: ... the rein was equilibrated in IP buffer with 1 mM DTT and incubated overnight at 4°C with 5 μL ProTEV Plus (Promega, Sunnyvale, CA) in 100 μL total reaction volume ...
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bioRxiv - Microbiology 2019Quote: ... In-gel digestion of selected protein bands or spots was performed overnight at 37 °C by incubation with trypsin (Sequencing grade, Promega, Madison, USA). Afterwards peptides were extracted as previously described [24] and samples were vacuum-dried using CentriVap Vacuum Concentrator (Labconco) ...
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bioRxiv - Microbiology 2021Quote: ... Growth curves were obtained in 96 wells microplates at 37°C and automatic recording of OD600 every 30 minutes using a plate reader (Promega GloMax Explorer).
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bioRxiv - Cancer Biology 2019Quote: Mitochondrial activity of C-33A cells was measured with the CellTiter 96 Aqueous One Solution Cell Proliferation Assay (MTS) (Promega, Madison, WI). Cells were seeded in 96-well plates (5000 cells/well ...
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bioRxiv - Cell Biology 2021Quote: ... The resulting protein samples were diluted to a final concentration of 2M Urea using 20 mM HEPES (pH 8) and digested overnight at 37°C using sequencing grade modified Trypsin (Promega, Madison, WI) in a 1 ...
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bioRxiv - Cell Biology 2021Quote: ... Primary antibodies were incubated in PBDS for 3 days at 4°C followed by 4 washes over the next 24 hours with 0.2% Tween-20 (Promega UK Ltd; H5151) in PBS ...
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bioRxiv - Microbiology 2022Quote: ... The light production was then monitored at 20-minute intervals at 37 °C for 2 hours using the Glomax 20/20 Luminometer (Promega, Madison, WI). A final measurement was also taken after the samples had been allowed to incubate overnight at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... The cells were then incubated for 24 hours at 37°C in 5% CO2 and the luciferase activity was measured using the ONE-Glo™ luciferase assay (Promega, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The cells were incubated at 37°C in 5% CO2 for 24 hours and luciferase activity was measured using a ONE-Glo™ luciferase assay (Promega, USA) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2020Quote: ... for 30 min at 37°C with shaking at 500 rpm in 500 μl of PBS with RNasin® Ribonuclease Inhibitors (Promega, #N261B). Negative control samples comprised 5 μg of RNA combined with HisPur Cobalt Resin (see details below) ...
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bioRxiv - Microbiology 2021Quote: ... The CaptureSelect Biotin anti-C-tag Conjugate (LifeTechnologies #7103252100; 1/4000) was mixed with Streptavidin Alkaline Phosphatase (Promega #V5591; 1/1000 dilution) for 10 minutes before use ...
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bioRxiv - Cancer Biology 2020Quote: ... and diluted to 1 M urea with 200 mM ammonium bicarbonate for trypsin digestion (1 µg, 37°C, 8 hr, Promega cat # V5113). After digestion ...
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bioRxiv - Neuroscience 2022Quote: ... we transferred the beads to 100 µL 50 mM Tris-HCl buffer with mixed with 1 µg trypsin/Lys-C (Promega Cat# V5073) per replicate ...
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bioRxiv - Systems Biology 2022Quote: ... 102.2 µL of 100 mM ABC was added and proteins were digested for 16 hours at 37°C using 5 µg of trypsin (dissolved in trypsin resuspension buffer, Promega, Walldorf, Germany). Tryptic digestion was stopped by addition of 2.5 µL 100% formic acid ...
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bioRxiv - Cell Biology 2020Quote: To compare Promega’s NanoBiT system β-arrestin2 was inserted at the C-terminal end of LgBiT using Promegas pBiT1.1-N vector (Promega Cat. #N2014) and the doubly palmitolyated fragment of GAP43 was inserted at the N-terminal of SmBiT using the pBiT2.1-C vector.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Cells were incubated with chemical treatments for 18 hours at 37°C prior to rinsing with PBS and lysing with 1x cell lysis buffer (Promega, Madison, WI). Luciferase activity was measured using a Berthold Centro XS3 LB 960 microplate luminometer with automatic injection of Luciferase Assay Reagent (Promega) ...
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bioRxiv - Molecular Biology 2020Quote: ... The band was cut and washed before the in-gel digestion of the proteins overnight at 37°C with a solution of modified trypsin (sequence grade, Promega, Charbonnières, France). The resulting peptides were extracted from the gel by one round of incubation (15 min ...
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bioRxiv - Microbiology 2019Quote: ... we replaced the C-terminal V5 tag with either HaloTag or NanoLuc (NLuc) from the NanoBRET Nano-Glo Detection System (Promega, Madison, WI). As a negative control ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were digested O/N at 37°C with mass spectrometry grade lysyl endopeptidase LysC and sequencing grade modified trypsin (Promega LTD, UK).
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bioRxiv - Synthetic Biology 2020Quote: ... and the bound proteins were subjected to on-cartridge digestion with mass spec grade Trypsin/Lys-C Rapid digestion enzyme (Promega, Madison, WI) at 70°C for 2h ...
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bioRxiv - Pathology 2021Quote: ... 30 μg of protein was digested with Lys-C (FUJIFILM Wako Chemicals Europe GmbH, Germany) for 4 h and subsequently with modified porcine trypsin (Promega, WI, USA) for 16 h at 37 °C.
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bioRxiv - Molecular Biology 2021Quote: ... cooled on ice to room temperature for 5 min and digested overnight at 37 °C with 0.5 μg of sequencing-grade trypsin (Promega Cat. No. V5111). Peptide mixtures were acidified to pH 3 with 1.5 µl 5% FA ...
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bioRxiv - Molecular Biology 2021Quote: ... and incubated overnight at 25 °C or endoproteinase GluC in a GluC/protein ratio of 1/20 (w/w) (Promega Cat. No.V1651) and incubated overnight at 37 °C ...
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bioRxiv - Microbiology 2021Quote: Protein samples were alkylated with 10 mM iodoacetamide in the dark at 37°C for 30m and subsequently digested with trypsin (Promega, 50 ng) overnight at 37°C ...