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Citations for Promega :
751 - 800 of 1323 citations for Rh Family C Glycoprotein RHCG Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... and goat anti-rabbit HRP conjugated antibody (Promega Cat # W4011). Blots were detected using Clarity Western ECL Substrate (Bio-Rad Cat# 1705061 ...
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bioRxiv - Molecular Biology 2023Quote: ... individual clones were immunoblotted using the anti-HaloTag antibody (Promega) to screen for the AGO2-HaloTag fusion of approximately 130 kDa molecular weight ...
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bioRxiv - Microbiology 2023Quote: ... and an anti-Rabbit IgG HRP-conjugated secondary antibody (Promega) (diluted 1:5000 in PBST ...
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bioRxiv - Cell Biology 2023Quote: ... secondary antibody used was anti-rabbit-HRP conjugate (W410B, Promega) (1:5000 dilution) ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were digested overnight at 37 °C with 5 μL of trypsin (1 μg dissolved in 50 mM HEPES pH 8.0, Promega V5111). The trypsin digestion was quenched by adding 4 μL of 1× EDTA-free protease inhibitor cocktail (Roche 11873580001) ...
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bioRxiv - Cell Biology 2020Quote: ... proteins were produced from pcDNA3-based plasmids at 30 °C for 90 min using the in vitro transcription & translation (IVTT) kit (Promega), following manufacturer’s instructions.
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bioRxiv - Neuroscience 2020Quote: ... corresponding samples (PSD-95 and MPP2) were incubated overnight at 37°C with 50 ng trypsin (sequencing grade modified, Promega) in 25 µl of 50 mM ammonium bicarbonate in the presence of heavy water (Campro Scientific GmbH ...
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bioRxiv - Cancer Biology 2019Quote: ... NSCs were then incubated for 45 minutes at 37°C in DNase I solution consisting of 20µl DNase I (Promega RQ1) in 250µl DNase buffer solution ...
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bioRxiv - Molecular Biology 2021Quote: ... FASP was carried out in two 10 kDa MWCO filters with a 50 mM iodoacetamide alkylation step and proteins were digested in 2M urea with 2% wt/wt Lys-C (Wako) for 6 h and 2% modified trypsin (Promega) for 12 h at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were subjected to digestion of Lys-C (Wako Chemicals) (1:50) for 2 h at RT and then to trypsin (Promega) (1:50 ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 µg of this RNA was treated for 10 minutes at 65 °C with a unit of RQ1 RNAse-free DNase (Promega) in a total reaction volume of 10 µl ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were incubated at 37°C for 1 hour and then proteolytic digestion was performed with LysC (Wako) for 4 hours and trypsin (Promega) overnight ...
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bioRxiv - Genomics 2020Quote: ... A double-stranded DNA adapter with a T-overhang was ligated for 1 h at 20°C and the resulting products were bead purified (ProNex, Promega), eluted and then split into two identical aliquots ...
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bioRxiv - Systems Biology 2020Quote: ... to a final concentration of 1% DOC before overnight digestion at 37 °C with lysyl endopeptidase (Wako Chemicals) and sequencing-grade porcine trypsin (Promega) at an enzyme-substrate ratio of 1:100 for both ...
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bioRxiv - Physiology 2019Quote: ... The concentrated urea was diluted 12 times with 50 mM ammonium bicarbonate buffer and proteins were digested for 18 hours at 36°C using trypsin/LysC mixture (Promega) in 1:50 (w/w ...
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bioRxiv - Molecular Biology 2019Quote: ... anti-m6A conjugated beads were incubated with purified mRNA with rotation at 4°C overnight in 300 µL MeRIP buffer with 1 µL RNase inhibitor (recombinant RNasin; Promega). 10% of the mRNA sample was saved as the input fraction ...
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bioRxiv - Bioengineering 2020Quote: ... In-solution protein digestion was carried out in a ratio of 1:25 w/w Trypsin/Lys-C (Mass Spectrometry Grade, Promega) to protein overnight at 37°C ...
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Quantitative proteomics reveals remodeling of protein repertoire across life phases of Daphnia pulexbioRxiv - Systems Biology 2019Quote: ... pH 8.5 and digested in a two-step process starting with an overnight digest at room temperature with Lys-C (Wako) followed by six hours of digestion with trypsin (sequencing grade, Promega) at 37 ° C ...
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bioRxiv - Synthetic Biology 2019Quote: ... Backbone and inserts were ligated either for 3 h at RT or overnight at 4°C using T4 ligase (Promega). All vector sequences (Table S5 ...
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bioRxiv - Biochemistry 2019Quote: ... Reactions (3 μL) were incubated for 60 min at 37 °C and quantified with the Nano-glo assay system (Promega).
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bioRxiv - Genomics 2019Quote: ... Tagmentation was performed on 250,000 HCT116 Parental and DKO cells using 700nM Tn5 Transposase assembled using preannealed Tn5ME-A_mC and Tn5ME-B_mC (Table S3) for 30 minutes at 37°C following the addition of 0.01ng of unmethylated Lambda DNA (Promega #D1521). We recommend performing a titration of Tn5 transposase to nuclei input to assay minimum amounts required as in Figure S5 ...
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bioRxiv - Pathology 2020Quote: ... Sample pH was adjusted to 7.5 with 0.05 M TEAB and proteins were digested overnight at 37 °C with sequencing-grade trypsin (Promega, Madison, WI) in 1:30 (w/w ...
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bioRxiv - Biophysics 2021Quote: ... Samples were incubated at 25°C for 30 minutes with gentle shaking before addition of 50 µL Kinase GloTM Max reagent (Promega) and further incubation at 25°C for 10 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... The sequences ligated by the T4 ligase at 4 °C for 12 h were cloned in the pGEM T-easy vector (Promega). The competent Escherichia coli X10-Gold (Stratagene ...
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bioRxiv - Biochemistry 2020Quote: ... cDNAs were obtained from Origene in pCMV6 vector that also incorporates C-terminal Myc and FLAG tags (Myc-FLAG) or subcloned into a pHTC (Promega)-derived vector that expresses C-terminal HaloTag (41) ...
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bioRxiv - Plant Biology 2021Quote: ... 2 μg of each purified plasmid (pTnT-SEP3-3FLAG and pTnT- AGIAP1-5MyC) were used as input in a 50 μl TnT reaction incubated at 25 °C for 2 hr (Promega). The reaction solution was then combined with 50 μl IP buffer (PBS supplemented with 0.005 % NP40 and proteinase inhibitors (Roche) ...
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bioRxiv - Biochemistry 2021Quote: ... 55 mM IAA at room temperature for 30 min in the dark and digested at 37°C for 16 h with 2 μg porcine trypsin (sequencing grade, Promega). The resultant tryptic peptides were extracted in 40% ACN/0.1% TFA into fresh Protein LoBind® microtubes ...
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bioRxiv - Cell Biology 2021Quote: ... alkylated with 5 mM iodoacetamide for 20 min at room temperature and digested overnight at 37 °C using 13 ng/μl trypsin (Promega). Digested peptides were loaded onto C18-Stage-tips (Rappsilber et al. ...
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bioRxiv - Plant Biology 2020Quote: ... The soluble fraction was then digested for 20 h at 37°C using a 1:50 ratio of trypsin (Promega) to extracted protein while gently shaking ...
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bioRxiv - Microbiology 2020Quote: ... and digested for 2 hours at 37°C with ApaI and SpeI restriction enzymes and ligated with T4 DNA ligase (Promega) overnight at 16° to a double-digested pKNG101 plasmid ...
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bioRxiv - Microbiology 2021Quote: ... After 30 min at 60°C the samples were cooled to room temperature and 4 μg of Sequencing Grade Trypsin (Promega) for digestion were added ...
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bioRxiv - Microbiology 2021Quote: ... samples were washed-dried once again and digested overnight at 37°C using mass spectrometry grade Trypsin gold (Trypsin Gold, Promega) at a concentration of 12.5 ng/μl in 50 mM ammonium bicarbonate and 10 mM CaCl2 ...
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bioRxiv - Microbiology 2020Quote: ... The cells were then left at 32°C or moved to 37°C for 9 hours before read on a luminometer using the Dual-Luciferase Reporter Assay Step (Promega) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Plates were frozen for at least 30 minutes at −80°C before thawing and reading on a GloMax Multi plate reader (Promega). Briefly ...
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A Bidirectional Switch in the Shank3 Phosphorylation State Biases Synapses toward Up or Down ScalingbioRxiv - Neuroscience 2021Quote: ... Proteins were digested with endoproteinase LysC (Wako Laboratories) for 2 h at 25 °C followed by overnight digest with sequencing-grade trypsin (Promega) at 25 °C (enzyme-to-substrate ratios of 1:50) ...
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bioRxiv - Cell Biology 2021Quote: ... which were washed with a series of harsh washes before cross-linker reversion and on bead digest with Trypsin Lys-C (Promega). Peptides were then desalted and prepared for mass spectrometry ...
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bioRxiv - Cell Biology 2021Quote: ... On-bead digestion was performed by overnight incubation at 37°C with 2 μg of mass spectrometry grade trypsin (Promega). The following morning an additional 2 μg of trypsin was added to each sample and incubated at 37°C for 4-6 hours ...
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bioRxiv - Cell Biology 2021Quote: ... while the sequences for NSD2 or NSD3 PWWP1 domain was cloned in-frame into a pNLF1-C [CMV/Hygro] vector from Promega using the In-Fusion HD Cloning kit (Takara) ...
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bioRxiv - Cell Biology 2021Quote: ... NY) and washed with S-Trap buffer prior to overnight proteolysis at 37°C with LysC/trypsin (Promega, Madison, WI) in 50 mM ammonium bicarbonate (pH 8.0 ...
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bioRxiv - Neuroscience 2020Quote: ... 8 min, 4 °C) and resuspended in staining buffer (PBS, pH 7.4, with 0.5% RNase-free BSA (Promega, Heidelberg, Germany) and 0.2 U/μl of RNasin Plus RNase inhibitor (Promega)) ...
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bioRxiv - Cell Biology 2020Quote: ... and digested overnight at 37°C with 1:50 enzyme:substrate ratio (wt/wt) of sequencing grade trypsin (Promega, Madison, WI) as described 59 ...
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bioRxiv - Biophysics 2020Quote: ... for 2 hours at 37° C and subsequently diluting to 1 M urea with 50 mM NH4HCO3 and finally adding 2% (w/w) trypsin (Promega) for 14 hours at 37° C ...
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bioRxiv - Cell Biology 2021Quote: ... for 1 hour at 37°C and DNA was extracted using the Wizard SV gel and PCR cleanup system (Promega). Input and immunoprecipitated DNA was subjected to quantitative PCR using the oligonucleotides shown in Table 1 ...
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bioRxiv - Cell Biology 2021Quote: ... Trypsin digestion was carried out overnight at 37°C with 1:50-1:100 enzyme-protein ratio of sequencing grade-modified trypsin (Promega) in 50 mM NH4HCO3 pH 7.5 ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins on beads were digested overnight at 37°C by sequencing grade trypsin (12.5 μg/ml; Promega Madison, Wi, USA) in 20 μl of NH4HCO3 25 mmol/l ...
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bioRxiv - Biochemistry 2022Quote: ... Samples were diluted with ddH2O 1:5 and proteins were digested for 20 h at 37°C using trypsin (Trypsin Gold, Promega) at an enzyme/protein ratio of 1:50 ...
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bioRxiv - Cell Biology 2022Quote: ... The cell plates were incubated at 37°C for 48-60 hours before 50 μL of CellTiter-Glo reagent (Promega) was added ...
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bioRxiv - Cell Biology 2022Quote: ... for 2 h followed by a second digest with trypsin at 35°C overnight at 1200 rpm (1:50 ratio w/w, Promega). Proteolysis was stopped by the addition of 1% (v/v ...
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bioRxiv - Cell Biology 2022Quote: ... Trapped proteins were washed four times with the methanol TEAB buffer and then digested overnight at 37°C using 1ug Trypsin (Promega). Digested peptides were eluted and dried in a vacuum centrifuge before LC-MS analysis.
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bioRxiv - Biochemistry 2022Quote: ... SpCas9 gRNAs were transcribed at 37 °C for 16 hours using the T7 RiboMAX Express Large Scale RNA Production Kit (Promega). For gRNAs utilized in in vitro cleavage reactions containing SpRY from human cell lysates ...