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Citations for Promega :
8401 - 8450 of 8541 citations for Nitrite Microplate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... germinated urediniospores and from rust infected leaves at 6 and 9 days post inoculation (dpi) was extracted using the Promega Maxwell® RSC Plant RNA Kit with a Maxwell® RSC instrument (Promega.com.au). The spores were induced for germination by placing on the surface of milli-Q water ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Evolved strains were thawed and grown overnight in 150 ml of liquid medium and DNA was extracted using the Wizard Genomic DNA Purification Kit from Promega (WI, USA). One DNA sample (20 to 60 µg ...
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bioRxiv - Evolutionary Biology 2021Quote: Viral RNA (vRNA) was extracted from 100 μl of VTM using the Viral Total Nucleic Acid Purification kit (Promega, Madison, WI, USA) on a Maxwell RSC 48 instrument and eluted in 50 μL of nuclease-free H2O.
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bioRxiv - Microbiology 2021Quote: ... Primary human airway epithelial cells were homogenized in homogenization solution supplemented with 1-thioglycerol and lysed by addition of lysis buffer (Maxwell 16 LEV simplyRNA cells kit; Promega #AS 1270). Prior to RNA extraction ...
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bioRxiv - Microbiology 2019Quote: ... RNA was extracted using the Maxwell 16 LEV simplyRNA tissue kit in combination with the Maxwell nucleic acid extraction robot (Promega, WI, USA), according to manufacturer’s instruction ...
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bioRxiv - Microbiology 2020Quote: ... the Wizard Genomic DNA Purification Kit was used including both lysozyme and lysostaphin (10 mg/ml each) (Ref. A1120, Promega Corporation, Spain); PacBio sequencing ...
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bioRxiv - Microbiology 2020Quote: ... All PCR products were purified with an AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, USA) and quantified using a QuantiFluor™-ST (Promega, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2019Quote: Genomic DNAs were isolated from whole blood EDTA samples with a Maxwell 16 instrument using Maxwell 16 LEV Blood DNA kits according to the manufacturer’s protocol (Promega, Madison, WI, USA). These genomic DNAs were used as templates for PCR with a panel of primers that flank the highly polymorphic peptide binding domains encoded by exon 2 of class I (Mamu-A ...
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bioRxiv - Microbiology 2022Quote: ... The PCR products were extracted from a 2% agarose gel and purified using AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, USA) and quantified by QuantiFluor™-ST (Promega, USA) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: Total genomic DNA was isolated from a piece of fin or muscle tissue using a Wizard Genomic DNA Purification kit (Promega, Tokyo, Japan) or DNeasy blood and tissue kit ...
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bioRxiv - Cancer Biology 2022Quote: ... Tumor pieces were placed in homogenizer tubes containing ceramic beads along with lysis buffer from the Total RNA Purification Kit (Norgen, 37500) and RNAse inhibitors (10 μl/mL β-mercaptoethanol and 200 units/mL RNAsin Plus (Promega, N2615)) ...
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bioRxiv - Developmental Biology 2022Quote: Genomic DNA was prepared from RV free wall and HD-area pieces using the Wizard® Genomic DNA Purification Kit (Promega, France). Samples were submitted to polymerase chain reaction analysis ...
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bioRxiv - Genetics 2022Quote: ... Total RNA was extracted from 14-day-old seedlings (12-days old for the cordycepin experiments) using the Maxwell 16 LEV Plant RNA Kit and Maxwell 16 Instrument (Promega, Madison, WI). For RT-PCR and qRT-PCR ...
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bioRxiv - Evolutionary Biology 2022Quote: ... gDNA was isolated from overnight cultures grown in the respective evolution medium inoculated by single evolved colonies using Wizard Genomic DNA purification kit (Promega, Madison, Wisconsin). We performed qPCR using Promega qPCR 2x Mastermix (Promega ...
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bioRxiv - Microbiology 2023Quote: ... United Kingdom) and genomic DNA from bacterial colonies was obtained using the Promega Wizard Genomic DNA Purification Kit (Promega, Madison WI, USA). DNA concentrations were measured with Nanodrop (Thermo Scientific ...
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bioRxiv - Microbiology 2022Quote: Genomic DNA was extracted at the NCPHL from subcultures inoculated with single bacterial colonies and grown in nutrient agar (Oxoid, USA) at 37°C overnight according to the manufacturer instructions (Wizard® Genomic DNA Purification kit, Promega, UK). Genomic DNA samples (derived from 10 environmental and 250 clinical samples ...
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bioRxiv - Microbiology 2024Quote: ... and the three randomly selected variants (AMA22_1 IHCA and AMA23 IHC AVI 4 μg/mL and R AVI 8 μg/mL) was performed following the manufacturer’s instructions using the Wizard Promega kit (Promega, Madison, WI, USA). For whole genome sequencing ...
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bioRxiv - Plant Biology 2024Quote: Total RNA was extracted from roots sampled as above using the SV total RNA isolation kit system following the manufacturer’s instructions (Promega, Madison, WI, USA). cDNA was synthesized with Superscript III reverse transcriptase (Invitrogen® ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: All split luciferase biosensors were made by In-Fusion® HD Cloning kit using firefly luciferase from the pGL3-basic vector (Promega, USA) template ...
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bioRxiv - Molecular Biology 2024Quote: ... tissue homogenization was carried out by bead beating and immediately followed by RNA extraction using the Maxwell® RSC simplyRNA Tissue Kit (Promega AS1340) as per manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: RNA was isolated from islets from ∼15-week-old male mice (WT and Kcnk16 L114P (L/P)) using Maxwell® 16 LEV simplyRNA Purification Kits (Catalog no. AS1280, Promega, USA). RNA integrity was analyzed using an Agilent 2100 Bioanalyzer ...
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bioRxiv - Microbiology 2024Quote: ... Complementary DNA (cDNA) synthesis was performed with oligo-dT primers and random hexamers using the First Strand cDNA Synthesis Kit (Promega, Madison WI) following the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: Total RNA was isolated from 1×106 cryopreserved lymph node (LN) mononuclear cells (LNMC) isolated from LN biopsies using the SimplyRNA Tissue kit for the Maxwell RSC instrument (Promega, Madison, WI). RNA was then tested for both SIV gag and a cellular gene ...
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bioRxiv - Molecular Biology 2023Quote: Genomic DNA was extracted from Tetrahymena WT (SB210, with or without BrdU-labeling) and ΔAMT1 cells using Wizard® Genomic DNA Purification Kit (Promega, A1120), sheared to 3-5kb in length with Megaruptor (Diagenode Diagnostics) ...
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bioRxiv - Microbiology 2022Quote: The extent of ADCC activation induced by human γ-globulins was evaluated with the use of an ADCC Reporter Bioassay (Core Kit; Promega, G7010) and the EnSpireMultimode Plate Reader (PerkinElmer) ...
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bioRxiv - Bioengineering 2023Quote: ... We then performed first-strand cDNA synthesis from 400 ng total RNA in 20 uL reactions using Promega GoScript Reverse Transcription Kit (Promega, catalog # A5000).
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bioRxiv - Molecular Biology 2023Quote: ... DNA for PCR was isolated from 50 µl of tissue lysate using the Maxwell RSC tissue DNA Kit (Promega, Madison, WI, USA).
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bioRxiv - Cancer Biology 2023Quote: Total RNA from MB cells treated with RA for 48 h was extracted using the SV Total RNA Isolation System kit (Promega, Madison, USA), following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... Single transformed ampicillin resistant colonies were grown in LB + Ampicillin and their DNA was extracted by the Wizard® Plus SV Minipreps DNA Purification System kit (Promega, A1460). Clones with correct insertions were evaluated by Sanger sequencing (LightRun tube ...
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bioRxiv - Genomics 2023Quote: Complete genomic DNA was extracted from fresh overnight growth on Nutrient agar (Oxoid, Hampshire, England, U.K) using a Wizard genomic DNA purification kit (Promega Corporation, Madison, USA) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: DNA in the HHMX-stimulated supernatants of THP1 dual reporter cells was quantified using the QuantiFluor dsDNA Detection Kit from Promega (catalog # E2670) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: RNA isolation from the cultured THP1 dual reporter cells was performed by using ReliaPrep RNA Cell Miniprep Kit (Promega, USA, catalog # Z6011) according to the instructions of the manufacturer ...
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bioRxiv - Microbiology 2023Quote: We isolated the genomic DNA of the strains used in this work using the Wizard genomic DNA purification kit (Promega, WI, USA) and quantified using the Qubit 4 fluorometer (ThermoFisher Scientific ...
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bioRxiv - Pathology 2024Quote: ... amniotic fluid and eluates from swab samples) using the Viral Total Nucleic Acid Purification Kit for the Maxwell RSC instrument (Promega, Madison WI) with the following modifications ...
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bioRxiv - Microbiology 2024Quote: ... The PCR products were purified with the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, USA) and quantified using Quantus™ Fluorometer (Promega, USA). Purified amplicons were pooled in equimolar amounts ...
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bioRxiv - Biochemistry 2024Quote: Total RNA was isolated from the cells of 6-well plates treated as above using the Maxwell RSC simplyRNA kit and the Maxwell RSC Instrument (both Promega, Southampton, UK) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... Two hundred ng RNA were used to prepare complementary DNA (cDNA) using M-MLV reverse transcriptase kit (Promega, Charbonnières-les-Bains, France) and oligo(dT ...
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bioRxiv - Cancer Biology 2021Quote: Genomic DNA from cell lines was isolated using Maxwell® 16 Instrument and the Maxwell® 16 DNA Purification Kit (Promega Cat.# AS1020) following the protocol provided ...
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Differential polysaccharide utilization is the basis for a nanohaloarchaeon : haloarchaeon symbiosisbioRxiv - Microbiology 2019Quote: ... Amplicons for cloning were cut out from the gel and purified with the Wizard SV Gel and PCR Clean–up System kit (Promega, Madison, WI, USA). The other Archaea present in the last positive enrichment were identified following the cloning and sequencing of the 16S rRNA gene using the conventional archaeal universal primers A20F-1492R42,43 ...
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bioRxiv - Genomics 2019Quote: Genomic DNA was isolated from 30 ul of chicken blood samples using the Promega Wizard Genomic DNA Purification Kit (Promega, Madison, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... a total of 1□μg of RNA for each sample was reverse-transcribed to cDNA using reverse-transcription kits (Promega, Madison, WI, USA), and real-time quantitative reverse transcription (qRT)-PCR was performed on a 7500 real-time PCR System (Applied Biosystems ...
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bioRxiv - Neuroscience 2019Quote: ... genomic DNA from puromycin selected cells and wild-type HEK293 cells was extracted (Promega Wizard Genomic DNA Purification Kit #A1120; Promega, Madison, WI, USA) for PCR amplification of a 535 bp region flanking the sgRNA cleavage site ...
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bioRxiv - Plant Biology 2019Quote: ... young and mature leaves of five plants (siblings of the plant used for genome sequencing) using the SV Total RNA Isolation Kit with on-column DNase I treatment (Promega, Madison, WI, USA). The RNA quantity and quality were assessed by RIN (RNA Integrity Number ...
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bioRxiv - Microbiology 2020Quote: ... for 20 min at 56°C before DNA was purified with Maxwell® RSC Blood DNA Kit (Promega Pte. Ltd., Cat. No. AS1400). DNA concentration was quantified using Qubit® 2.0 fluorometer ...
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bioRxiv - Microbiology 2020Quote: ... of luciferase was determined using a PicaGene Luminescence Kit (TOYO B-Net Co., LTD, Tokyo, Japan) and GloMax Navigator System G2000 (Promega Corporation, Madison, WI), according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2020Quote: ... and DNA-concentration was determined using a QuantiFluor® dsDNA kit (detection limit: 50 pg/mL; sensitivity: 0.01 – 200 ng/µL) and Glomax® –Multi+ system (Promega, Madison, WI) with the blue fluorescence optical kit installed (Ex ...
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bioRxiv - Microbiology 2020Quote: ... Extraction of total RNA from viral transport media was performed using the Maxwell RSC Whole blood RNA/DNA kit (Promega Corporation, Madison, WI) on the Maxwell RSC 16 automated nucleic acid extractor ...
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bioRxiv - Microbiology 2020Quote: Faecal DNA was extracted and purified using a combination of repeated bead-beating (method 5) (Costea et al, 2017) and the Maxwell 16 Tissue LEV Total RNA Purification Kit (Promega, Maddison, WI, USA), with STAR (Stool transport and recovery ...
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bioRxiv - Plant Biology 2020Quote: ... and five early developmental buds (one early developmental sample) per individual using the Maxwell 16 LEV Plant RNA Extraction Kit (Promega Corporation, Madison, WI).
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bioRxiv - Molecular Biology 2020Quote: ... and then cloned to ensure representative amplification of the parental copies using pGEM-T Easy-cloning Vector Kit (Promega Corp., Madison, WI, USA) and transformed into competent Escherichia coli JM109 at 42 °C ...