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Citations for Promega :
8201 - 8250 of 8541 citations for Nitrite Microplate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: The total RNAs from each cell line (LO2 and Nrf1-/- LO2 cells) were isolated and purified with an RNA extraction kit (Promega) and subsequently all samples were sequenced at the Beijing Genomics Institute (Shenzhen ...
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bioRxiv - Cell Biology 2023Quote: ... Necrosis was quantified by measuring release of lactate dehydrogenase in 30 µl samples of the medium and apoptosis by determining cell-associated Caspase3/7 activity using the respective kits from Promega Corp. ...
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bioRxiv - Plant Biology 2024Quote: ... Total RNA was isolated from 30 mg of ground plant tissue using the SV Total RNA Isolation System Kit (Promega) and its concentration and integrity were assessed using the RNA 6000 Nano Kit on a Bioanalyzer (Agilent Technologies) ...
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bioRxiv - Biochemistry 2023Quote: ... Authentication of this cell line was performed by the Johns Hopkins Genetics Resources Core Facility by short tandem repeat analysis using the PowerPlex 16 HS kit (Promega) within a year of study ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Bacterial genomic DNA was extracted from static overnight cultures of WS clones using the Wizard Genomic DNA purification kit (A1125, Promega) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: Stereocaulon virgatum RNA was extracted from the fungal culture using the Maxwell® RSC Plant RNA Kit (Promega, Madison, WI). Tissue was homogenized in 30 second intervals for 1.5 minutes at 30hz ...
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bioRxiv - Microbiology 2024Quote: ... Total DNA from feather pulps and tracheas were extracted using the MagaZorb® DNA mini-prep kit (Promega, Madison, WI), following the manufacturer’s recommendations with a few modiications ...
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bioRxiv - Microbiology 2024Quote: ... The bacteria were pelleted by centrifugation and DNA extraction was performed with the Wizard® genomic DNA purification kit (Promega).
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bioRxiv - Developmental Biology 2024Quote: ... The 4 µg of linearized vectors were transcribed 5h using RiboMAX™ Large Scale T7 RNA polymerase kit (Promega, #P1300) with 5.6 mM Ribo m7G Cap Analog (Promega ...
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bioRxiv - Microbiology 2024Quote: ... In vitro mRNA synthesis was then carried out at 37 °C for 4 h in T7 RiboMAX Large Scale RNA Production System kit (Promega) according to the manufacturer’s recommendations ...
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bioRxiv - Developmental Biology 2024Quote: ... Lysates were diluted 1:10 with Milli-Q water and processed for PCR using GoTaq G2 Flexi DNA Polymerase kit (Promega). Reactions were placed in a thermocycler on a touchdown program ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and used the GoScript Reverse Transcription System kit to generate cDNA from extracted RNA (Catalog #A5000, Promega Corporation, Madison, WI). We designed primers using the tool primer-Blast134 and the predicted cDNA sequence of kitlga (Fig ...
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bioRxiv - Neuroscience 2024Quote: ... RNA was isolated from the cell lysis using Maxwell® RSC simply RNA Cells/Tissue Kit (Promega, Madison, WI, USA) and cDNA was synthesized ...
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bioRxiv - Microbiology 2024Quote: Genomic DNA was extracted from bacterial cells grown overnight in MOKA-rich medium using the Wizard genomic DNA purification kit (Promega) or as described (62 ...
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bioRxiv - Developmental Biology 2024Quote: The efficacy of all MOs was verified in western blots following in vitro transcription and translation (TNT-coupled Wheat Germ Extract kit, Promega) of pCMV-Sport6-marcks.S ...
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bioRxiv - Molecular Biology 2024Quote: ... Macroscopic dissection of tumor areas was performed with sterile scalpels and tissues were extracted using the Maxwell RSC FFPE Plus DNA Purification Kit (Promega) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2021Quote: ... Culture media was then replaced with 50 µL of qNSC/aNSC media containing 1 mM 2DG (2-deoxy-D-Glucose, provided in Glucose Uptake-Glo kit from Promega (J1342)) reagent for 10 minutes in incubator (humidified ...
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bioRxiv - Cell Biology 2020Quote: ... and SERBP1 were amplified from human cDNA and cloned into the appropriate vectors (pACT or pBIND) provided by the CheckMate Mammalian Two-Hybrid kit (Promega E2440). The appropriate combination of edited pACT and pBIND vectors ...
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bioRxiv - Genetics 2021Quote: ... Approximately 1 μg RNA per sample was converted to cDNA using the ImProm-II Reverse Transcription System kit (Promega, Madison, WI) using the Oligo(dT)15 primer supplied with the kit and with a final concentration of 3 mM MgCl2 according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... the fluorescence derived from the incorporation of BRYT Green® Dye into the double-stranded PCR products was measured at the end of each cycle using the GoTaq® qPCR Master Mix 2X Kit (Promega). The results were analysed using Bio-Rad CFX Maestro software ...
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bioRxiv - Microbiology 2019Quote: ... from 25 randomly selected CM samples was extracted by an automated Maxwell 16 DNA extraction platform using blood DNA purification kits (Promega, UK) following previously described protocols2 ...
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bioRxiv - Microbiology 2019Quote: ... Viral RNA was isolated from homogenized tissues using the Maxwell 48 RSC Viral Total Nucleic Acid Purification Kit (Promega, Madison, WI) on a Maxwell 48 RSC instrument ...
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bioRxiv - Neuroscience 2021Quote: RNA was extracted from 10 flies for each experiment condition and the process followed the protocol of Promega ReliaPrepTM RNA Tissue Miniprep System kit (Promega, Z6110). Extracted RNA was stored in a −70°C refrigerator ...
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bioRxiv - Biophysics 2022Quote: ... The resulting 2.6-kb fragment was separated from the 1.4-kb fragment and uncut plasmid by agarose gel purification (Promega Gel Wizzard Kit). A set of oligonucleotides that form a biotinylated and primed fork was ligated to one end of the fragment and the final product purified on a Sepharose-4B column as previously described [42] ...
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bioRxiv - Microbiology 2022Quote: ... A total of 56 Fusobacterium isolates were recovered and subjected to DNA extraction using the Wizard genomic extraction kit (Promega, USA). For each isolate ...
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bioRxiv - Plant Biology 2019Quote: ... 1 g of fresh leaves were collected from each genotype and DNA was extracted using the Wizard Genomic DNA Purification Kit (Promega, USA) following manufacturer instructions ...
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bioRxiv - Plant Biology 2019Quote: Total of RNA was extracted from various tissue of plants by using the Eastep®Super Total RNA Extraction Kit (Promega). The cDNA was synthesized from Dnase I treated total RNA using M-MLV 1st Strand Kit (Invitrogen) ...
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bioRxiv - Synthetic Biology 2019Quote: Liquid cultures of the transformed cells were used for plasmid extraction and purification using the Wizard® Plus SV Minipreps DNA Purification System kit (Promega). The plasmids quality and concentration were quantified using the Synergy HTX plate reader (BioTek ...
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bioRxiv - Developmental Biology 2021Quote: ... pCDNA-Cnot3 Δ701-751 and pCDNA-Cnot3Δ651-751 were in vitro transcribed/translated using a TNT® Quick Coupled Transcription/Translation kit (Promega, USA) according to manufacturer’s instructions using 10μCi of 35S-Methionine to radiolabel the proteins ...
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bioRxiv - Genetics 2021Quote: ... of G1 and G2 mosquitoes fluorescently expressing the actin5c::eCFP construct was extracted as individual samples using the Wizard® Genomic DNA Purification Kit (Promega). Regions of gDNA were then amplified according to the three primer pairs as indicated in Fig ...
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bioRxiv - Systems Biology 2021Quote: ... 2 ul were used as templates for PCR with the srrm3 or srrm4 primers designed to amplify the genomic region of interest (listed in Table S12) and GoTaq® Flexi DNA Polymerase kit (Promega).
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bioRxiv - Biochemistry 2021Quote: ... liquid cultures were pelleted at 3000 g for 10 minutes and miniprepped using the Tecan Fluent robotic liquid handler with Promega Wizard SV 96 Plasmid DNA Purification Kit (Promega; A2250).
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bioRxiv - Immunology 2021Quote: Around 1×105 cells were treated as indicated prior to lysis and RNA isolation using the ReliaPrep™ RNA Miniprep kit (Promega). Approximately 1μg of total RNA was reverse transcribed into cDNA using SuperScript III (Invitrogen ...
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bioRxiv - Biochemistry 2020Quote: miRNAs were isolated from ultracentifuged EVs resuspended in 25 ul of phosphate buffered saline (PBS) using the Maxwell® RSC miRNA Plasma and Serum Kit (AS1680, Promega) following the manufacturer’s instruction ...
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bioRxiv - Microbiology 2020Quote: ... The duplicate amplification products of each sample were pooled and purified with the SV Wizard PCR Purification kit (Promega, Madison, WI) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... hLamp1-mCherry (this study) were prepared using the Ambion mMessage Machine kit using Sp6 (#AM1340) supplemented with RNAse Inhibitor (Promega #N251B), and diluted to 30–80ng/μl for injection into embryos ...
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bioRxiv - Developmental Biology 2020Quote: ... The α-tub∷GFP (Chung et al., 2014) plasmid was injected at 5-10ng/μl after purification using the PureYield Midiprep kit (Promega, #A2492).
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bioRxiv - Zoology 2021Quote: ... The PCR products were detected through 2% agarose gel electrophoresis and purified with an AxyPrep DNA gel recovery kit (Axygen Biosciences, Union City, CA, USA) and then quantified with a QuantiFluor(tm)-ST blue fluorescence quantitative system (Promega, USA). Purified amplicons were pooled in equimolar amounts ...
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bioRxiv - Cell Biology 2021Quote: In vitro translated (IVT) proteins were generated in rabbit reticulocyte lysate using the SP6 TNT Quick-Coupled Transcription/Translation kit (Promega, L2080) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... PCR fragments amplified from genomic DNA extracted from the different founder mice using the Wizard SV genomic DNA extraction kit (Promega, A2360), were cloned into a vector using the TOPO TA cloning kit (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2021Quote: ... was synthesized from 1 μg of total RNA, using oligo-dT primers (USB Corporation, Cleveland, USA) and a reverse transcriptase kit (ImPROm-II, Promega, USA), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA was extracted from lung tissue using the Maxwell automated instrument and the Maxwell® RSC Viral Total Nucleic Acid Purification Kit (Promega). The concentration of RNA and quality were measured with HS Total RNA 15nt.methods (Agilent ...
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bioRxiv - Cancer Biology 2022Quote: ... GB02 and GB03 using the Maxwell® 16 Instrument with the Maxwell® 16 Tissue DNA Purification Kit (Promega, Madison, WI). DNA concentration was determined using the Qubit Fluorometer (Life Technologies ...
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bioRxiv - Molecular Biology 2022Quote: ... or 1/6th of the diaphragm) using a Maxwell RSC Instrument and Maxwell RSC simplyRNA Tissue Kit (both Promega, Southampton, UK) and complementary DNA (cDNA ...
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bioRxiv - Synthetic Biology 2022Quote: ... The yeast colony was grown overnight in 10 ml of YPD and the gDNA was extracted using the Wizard Genomic DNA Isolation kit (Promega, Switzerland). The PCR reaction was as follows ...
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bioRxiv - Molecular Biology 2022Quote: DNA extraction was also done for all the samples using the Wizard® Genomic DNA Purification Kit according to the manufacturer’s protocol (Cat#A1120, Promega USA).The eluted DNA was quantified by QuantusTM Fluorometer® (Cat# E6150 Promega Technologies ...
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bioRxiv - Microbiology 2022Quote: ... the cells were lysed and firefly and Renilla luminescence were measured following the manufacturer’s directions (Dual luciferase kit, Promega, Madison, WI) on a luminometer (GloMax® 20/20 Luminometer ...
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bioRxiv - Genomics 2022Quote: ... The liquid cultures were centrifuged at 10,000 rpm and DNA was extracted from the pelleted cells using Wizard DNA purification kit (Promega, Madison, USA) as per the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... DNA was extracted from 5 mL of overnight cultures using the Wizard Genomic DNA Purification Kit (Promega Corp., Madison, WI, USA) and DNA concentration was measured using a Qubit™ Fluorometer and Nanodrop 2000 (Thermo Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Remaining ATP was readout after 3 h incubation using Kinase-Glo® Luminescent Kinase kit following manufacturer’s instructions (Promega, WI, USA).