Labshake search
Citations for Promega :
8001 - 8050 of 8541 citations for Nitrite Microplate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... of blood sample was collected from each individual and genomic DNA was extracted by using Maxwell RSC whole blood DNA extraction kit (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... Genomic DNA was extracted from selected clones as well as in the parental clone using the Wizard Genomic DNA Purification Kit (Promega). NGS was performed using Nextera XT DNA Library Preparation kit and the NextSeq 500 sequencing systems (Illumina ...
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bioRxiv - Genomics 2020Quote: The total RNA was extracted from Inner Mongolia Alashan cashmere goats and Dazu black goats using RNA extraction Kit (Promega), and RNA quality and concentration were measured on an Agilent 2100 Bioanalyzer (Germany) ...
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bioRxiv - Microbiology 2019Quote: ... 2 mL of cultures grown to early stationary phase and genomic DNA extracted with a Wizard Genomic DNA Purification Kit (Promega). Outside-in colony PCR of a subset of hpn genes was performed with PrimeSTAR DNA polymerase (TaKaRa ...
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bioRxiv - Bioengineering 2019Quote: ... Cultures were grown for 16 h as described above and then 1 mL of culture was used to isolate DNA using the Wizard DNA Purification Kit (Promega). A Nanodrop 2000 (Thermo Scientific ...
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bioRxiv - Microbiology 2019Quote: ... All DNA extractions were done with the Wizard® SV Gel and PCR Clean Up system kit (Promega, Madison, USA).
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bioRxiv - Evolutionary Biology 2019Quote: DNA was extracted from stickleback fin clips using the Wizard® SV Genomic Purification kit (Promega® Corp., Madison, USA) and quantified using Picogreen® ds DNA assay (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... Post this total RNA was extracted from the treated and control insects using SV Total RNA Isolation kit (Promega, USA). The RNA was quantified and qualified by a NanoDrop ND-2000 (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2019Quote: ... Beads were washed four times with IP buffer and bound nucleic acids were isolated using the Wizard SV gel and PCR clean-up kit (Promega). Collected DNA fragments were quantified using RT-PCR as described above with the following primers ...
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bioRxiv - Biochemistry 2019Quote: ... The luminescence signal was developed for 20 min after adding 40 µl kinase detection reagent (kit) and measured with a GloMaxMulti plate reader (Promega). The average of three experiments performed in duplicate and normalized to PI3KC3-C1 activity were determined ...
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bioRxiv - Genomics 2019Quote: Genomic DNA was isolated from the tip of tails of 3-week-old mice using Wizard Genomic DNA purification Kit (Promega), amplified by PCR ...
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bioRxiv - Microbiology 2019Quote: ... Lifted cells were resuspended with an additional 50 µl of DMEM before adding to 50 µl Nuclei Lysis Buffer (Wizard Genomic DNA Purification kit; Promega) and frozen at −20°C overnight ...
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Global genetic patterns reveal host tropism versus cross-taxon transmission of bat BetacoronavirusesbioRxiv - Evolutionary Biology 2020Quote: Genomic RNA was extracted from small intestine and large intestine samples using the SV Total RNA Isolation kit (Promega, USA) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and from the interior of the sponge tissue (<2mm from the exterior pinacoderm) and the seawater filter papers using the Wizard Genomic DNA Purification Kit (Promega).
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bioRxiv - Plant Biology 2020Quote: ... only root parts (around 100 mg) were collected and total RNA was extracted using a Trizol-adapted ReliaPrep RNA Tissue Miniprep Kit (Promega). For verifying the transcript level in single T-DNA lines ...
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bioRxiv - Genetics 2019Quote: In vitro transcription and translation were realized from 500ng of cDNA pGEM-T construct using the TnT T7 Quick Coupled Transcription/Translation kit (Promega) and Transcend Biotin-Lysine-tRNA following the manufacturer protocol ...
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bioRxiv - Genetics 2021Quote: ... The genomic DNA (gDNA) of individual transient G0 mosquitoes was extracted using the Wizard® Genomic DNA Purification Kit (Promega). In the T7 Endonuclease I (T7EI ...
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bioRxiv - Microbiology 2021Quote: ... Approximately 4 μg of the extracted material was then used as template to in vitro synthesized viral genomic RNA transcripts using the Ribomax T7 RNA transcription Kit (Promega) and Ribo m7G Cap Analogue (Promega ...
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bioRxiv - Cell Biology 2021Quote: ... On day eight representative photographs were taken using an EVOS XL microscope and viability was assayed using the CellTiter-Glo Luminescent Cell Viability kit (Promega) per the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... Cell death was determined according to the activity of lactate dehydrogenase (LDH) in the culture supernatants using a CytoTox1 Kit according to the manufacturer’s instructions (Promega, USA).
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bioRxiv - Plant Biology 2020Quote: ... Nucleic acids deriving from PCRs or restriction digests reactions were purified from 1% TAE agarose gels with the “Wizard SV Gel and Purification System Kit” (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: RNA was extracted from nasopharyngeal swab samples using Maxwell® 16 Viral Total Nucleic Acid Purification Kit (Promega, Wisconsin-USA) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: Total mRNAs used for qRT□PCR experiments were isolated from Chlamydomonas using the Eastep Super total RNA extraction kit (Promega), and cDNA was synthesized with the PrimeScript TMRT Master Mix kit (TaKaRa) ...
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bioRxiv - Microbiology 2020Quote: A random-primers based reverse transcription of 1 μg of RNA was performed with the A3500 Reverse Transcription System Kit (Promega), followed by quantitative real-time PCR on cDNA using the FastStart Essential DNA Green Master kit (Roche ...
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bioRxiv - Plant Biology 2020Quote: ... were collected from each genotype and total RNA was extracted using a Trizol-adapted ReliaPrep RNA Tissue Miniprep Kit (Promega). Reverse transcription was carried out with PrimeScript RT Master Mix (Takara) ...
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bioRxiv - Plant Biology 2021Quote: ... ground in liquid nitrogen and total RNA from approximately 150 mg of ground leaf material was extracted with a ReliaPrep miRNA Cell and Tissue Miniprep System kit (Promega). A stem loop pulsed RT-PCR protocol [8 ...
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bioRxiv - Neuroscience 2020Quote: ... Cell viability was also determined based on lactate dehydrogenase (LDH) activity in conditioned culture media using a commercial kit (G1780, Promega) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: High molecular weight DNA was extracted using the Maxwell® RSC PureFood GMO and Authentication Kit as for qPCR (Promega). The high molecular weight DNA was sent to the Functional Genomic Center Zurich (FGCZ ...
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bioRxiv - Microbiology 2021Quote: ... Approximately 4 μg of the extracted material was then used as template to in vitro synthesized viral genomic RNA transcripts using the Ribomax T7 RNA transcription Kit (Promega) and Ribo m7G Cap Analogue (Promega ...
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bioRxiv - Microbiology 2021Quote: ... Genomic DNA was also extracted from inactivated grown cultures using a DNA extraction kit according to the manufacturer’s instructions (Promega, USA).
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bioRxiv - Microbiology 2021Quote: ... MT4 and TMZR5 cells were transduced with the genes in Fig 2G as before and 96 hours post-transduction the supernatants were harvested to measure toxicity of the expressed ISGs using the CytoTox-Glow kit (Promega). In a separate experiment ...
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bioRxiv - Molecular Biology 2020Quote: ... phaffii cells were cultured for 12-14 h and total RNA was isolated using RNA isolation kit (Cat. # Z3100, Promega) as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: gDNA was extracted from stationary phase evolved cocultures following revival from frozen stocks using a Wizard Genomic DNA purification Kit (Promega). DNA fragment libraries were constructed for samples from shaking WT-based cocultures A-F and NifA*-based cocultures M-R at generation ∼146 using NextFlex Bioo Rapid DNA kit ...
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bioRxiv - Microbiology 2019Quote: ... the aptamer pool from the 10th round of SELEX was cloned with the pGEMT-easy Cloning and Ligation Kit (Promega). To investigate the effect of counter-selection on the aptamer pools ...
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bioRxiv - Microbiology 2019Quote: Genomic DNA was extracted from loopfuls of pure MTBC colonies grown on Lowenstein-Jensen media (37) using the Maxwell 16 DNA Purification Kit (Promega). DNA from Nigerian strains was extracted using the Cetyl trimethylammonium bromide (CTAB ...
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bioRxiv - Cell Biology 2021Quote: ... genomic DNA from Proteinase K-digested mouse toes was extracted using the Wizard SV genomic DNA extraction kit (Promega, A2360). PCR reactions to amplify a fragment containing the target sites were carried out using the forward and reverse primers (Forward primer ...
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bioRxiv - Cell Biology 2021Quote: ... The RNA concentrations were measured and 1ug RNAs were used to synthesize cDNA using the GoScript Reverse Transcription System kit(Promega).
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bioRxiv - Cancer Biology 2021Quote: ... cells using ITX2 transfection reagent and luciferase activity (renilla and firefly) was measured 24hours later with the Dual Glow-Stop and Glow luciferase kit (Promega) using a BioTek luminometer (Synergy).
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bioRxiv - Cancer Biology 2021Quote: ... The desired PCR fragments were isolated from a 1% agarose gel and purified using the Wizard Genomic DNA Purification Kit (Promega). In order to identify the specific edition ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... PCR products were isolated with a QIAquick Gel Extraction kit (Quiagen) and purified fragments cloned into pGEM-T Easy vector (Promega). Specific details for the generated probes are as follow (gene name (accession number) ...
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bioRxiv - Molecular Biology 2022Quote: ... The SELENOP 3’UTR and the control plasmid were linearized using Bsb 1 and in vitro transcribed using the Ribomax kit (Promega). The RNA was then purified using p30 size exclusion columns (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... DNA extractions were carried out using the Wizard® Genomic DNA Purification Kit (Promega, Madison, WI, United States of America) following Lehner et al ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was eluted from the beads using the elution buffer (1% SDS and 100 mM NaHCO3) and purified by PCR purification kit (Promega). The final products were analyzed by quantitative real-time PCR with the primers listed below:
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bioRxiv - Plant Biology 2022Quote: ... Samples representing different stages of the parasitizing process (i.e. root development) were extracted using the Maxwell® RSC Plant RNA kit (Promega) with the Maxwell® RSC Instrument (Promega) ...
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bioRxiv - Biochemistry 2022Quote: ... SpCas9 gRNAs were transcribed at 37 °C for 16 hours using the T7 RiboMAX Express Large Scale RNA Production Kit (Promega). For gRNAs utilized in in vitro cleavage reactions containing SpRY from human cell lysates ...
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bioRxiv - Microbiology 2022Quote: ... Infected cells were incubated for approximately 18-20 hours and luciferase was measured using the Promega BrightGlo luciferase kit following manufacturers’ instructions (Promega). Entry signal was normalized to the average signal for the “no spike” control ...
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bioRxiv - Microbiology 2022Quote: The total RNA of tissues was extracted using the Maxwell RSC simply RNA tissue kit (AS1340, Promega, Madison, WI, USA) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... HCT116 cell line was treated for 24 h with 10 µM curcumin and RNA was isolated using the Maxwell® 16 IVD system and the Maxwell Simply RNA Tissue Kit according to the manufacturer’s instructions (Promega). RNA was reverse transcribed using the SuperScript® VILO cDNA Synthesis Kit (Invitrogen ...
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bioRxiv - Immunology 2022Quote: The genomic DNA of the 5 assessed M strains were isolated and purified using the Wizard Genomic DNA Purification Kit (Promega). The growing of strains ...
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bioRxiv - Cancer Biology 2022Quote: GSH/GSSG measurement was performed by a glutathione S-transferase (GST) enzyme coupled reaction using (GSH/GSSG Glo kit, Promega). Cells (2×104 ...