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Citations for Promega :
8051 - 8100 of 8213 citations for QuantiChrom Indole Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... The entire volume was extracted using a Promega Maxwell RSC Whole Blood DNA Kit on the Promega Maxwell RSC 48 (Promega, Madison, WI). Accurate DNA concentration was measured by Qubit fluorometer double-stranded DNA high sensitivity (dsDNA HS ...
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bioRxiv - Microbiology 2021Quote: ... PA) and AccuMap™ low pH protein digestion kit (with trypsin and lysC) and chymotrypsin (sequencing grade) were from Promega (Madison, WI). PreScisson protease was from GenScript (Piscataway ...
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bioRxiv - Neuroscience 2021Quote: ... Cytotoxicity was determined according to the activity of lactate dehydrogenase (LDH) in the culture supernatants using a CytoTox® Kit (Promega, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Positive clones were cultured in liquid LB medium supplemented with kanamycin (50 μg mL−1) for extraction of plasmid DNA with the Wizard Plus SV Minipreps DNA Purification System Kit (Promega - Madison, USA). Plasmids with unique EcoRI/HindIII restriction patterns were subjected to re-transformation and phenotypic confirmation by streaking the clones in solid LB medium supplemented with kanamycin (50 μg mL−1) ...
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bioRxiv - Microbiology 2021Quote: ... baumannii strain AYE (ATCC BAA-1710) was then similarly amplified from genomic DNA prepared with the Wizard Genomic DNA Extraction Kit (Promega, Madison, WI) using primers “F AdeC” and “R AdeC” and cloned downstream from the vector pLac site and Shine-Delgarno sequence in pBMTL-2NTC using vector primers ...
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bioRxiv - Pathology 2022Quote: ... or 300 uL urine+DMSO using the Maxwell RSC Viral Total Nucleic Acid Purification Kit on the Maxwell 48 RSC instrument (Promega, Madison, WI).
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bioRxiv - Cancer Biology 2022Quote: DNA and RNA were isolated using the Maxwell® RSC Cultured Cells DNA and the Maxwell® RSC simplyRNA Cells Kit (Promega) according to manufacturer’s instruction ...
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bioRxiv - Physiology 2022Quote: ... DNA was isolated from snap-frozen ACA tissues with the Maxwell® 16 Tissue DNA Purification Kit (#AS1030, Promega, Madison, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: Genomic DNA was isolated from 250μL of whole blood using a Maxwell® 48 LEV Blood DNA Kit (Promega Corporation, Fitchburg, WI). Following isolation ...
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bioRxiv - Cell Biology 2019Quote: ... or EED that was in vitro-transcribed and translated according to the manufacturer’s procedures (TNT T7 Quick Coupled Transcription/Translation Kit; Promega, Leiden, the Netherlands) at 4°C overnight ...
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bioRxiv - Cancer Biology 2020Quote: Total RNA was extracted from approximately 106 cells via the Maxwell 16 LEV simply RNA Tissue Kit (Cat. #AS1280, Promega, Madison, WI) on a Maxwell 16 Instrument (Cat ...
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bioRxiv - Microbiology 2019Quote: ... antibody-dependent cell-mediated cytotoxicity (ADCC) bioassays (29) were performed as prescribed by the manufacturer (ADCC reporter bioassay, complete kit, Promega, cat # G7010). In brief ...
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bioRxiv - Genetics 2020Quote: ... Dog DNA was isolated from clinical blood samples provided by CEU Cardenal Herrera Veterinary Hospital of University of Valencia using the Wizard Genome DNA Purification Kit (Promega, WI, USA). The use of dog blood samples was approved by the Animal Experimentation Ethics Committee of CEU Cardenal Herrera University of Valencia ...
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bioRxiv - Genetics 2020Quote: The RNA was isolated using a Maxwell® 16 AS2000 instrument with a Maxwell® 16 LEV simply RNA Tissue Kit (both Promega) following the recommended protocol ...
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bioRxiv - Genomics 2020Quote: Genomic DNA was extracted from whole blood obtained from GALA II study subjects using the Wizard Genomic DNA Purification kits (Promega, Fitchburg, WI), and DNA was quantified by fluorescent assay ...
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bioRxiv - Microbiology 2020Quote: DNA was extracted from this concentrated virus sample using a modified protocol with the Wizard Genomic DNA Purification Kit A1120 (Promega, Madison, WI). Briefly ...
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bioRxiv - Cancer Biology 2020Quote: In vitro translation of full-length SREBP1a and full-length SREBP1c was carried out using the TNT rabbit reticulocyte lysate translation kit (Promega, Wisconsin, USA) as per the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: Total RNA was isolated from tomato freeze-dried tissue (seedlings, leaves or fruit pericarp) using the Maxwell® RSC Plant RNA Kit with the Maxwell® RSC Instruments (Promega) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted from these bacterial suspensions using a Maxwell® 16 automated nucleic acid purification system and the Maxwell® tissue DNA purification kit following the manufacturer’s instructions (Promega, UK). Purified DNA extracts were treated with RNase (New England BioLabs ...
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bioRxiv - Microbiology 2019Quote: Viral RNA was isolated from plasma samples using the Maxwell 16 Viral Total Nucleic Acid Purification kit on the Maxwell 16MDx instrument (Promega, Madison WI). Viral RNA was then quantified using a highly sensitive qRT-PCR assay based on a previously published protocol115.
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bioRxiv - Plant Biology 2019Quote: Extraction of genomic DNA from infected and healthy plant materials was performed using the Wizard Genomic DNA purification kit (Promega, Madison, WI); DNA was isolated from bacterial cultures using the Ultra Clean Microbial DNA Isolation kit (Mo Bio. ...
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bioRxiv - Genomics 2021Quote: ... germinated urediniospores and from rust infected leaves at 6 and 9 days post inoculation (dpi) was extracted using the Promega Maxwell® RSC Plant RNA Kit with a Maxwell® RSC instrument (Promega.com.au). The spores were induced for germination by placing on the surface of milli-Q water ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Evolved strains were thawed and grown overnight in 150 ml of liquid medium and DNA was extracted using the Wizard Genomic DNA Purification Kit from Promega (WI, USA). One DNA sample (20 to 60 µg ...
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bioRxiv - Evolutionary Biology 2021Quote: Viral RNA (vRNA) was extracted from 100 μl of VTM using the Viral Total Nucleic Acid Purification kit (Promega, Madison, WI, USA) on a Maxwell RSC 48 instrument and eluted in 50 μL of nuclease-free H2O.
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bioRxiv - Microbiology 2021Quote: ... Primary human airway epithelial cells were homogenized in homogenization solution supplemented with 1-thioglycerol and lysed by addition of lysis buffer (Maxwell 16 LEV simplyRNA cells kit; Promega #AS 1270). Prior to RNA extraction ...
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bioRxiv - Microbiology 2019Quote: ... RNA was extracted using the Maxwell 16 LEV simplyRNA tissue kit in combination with the Maxwell nucleic acid extraction robot (Promega, WI, USA), according to manufacturer’s instruction ...
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bioRxiv - Microbiology 2020Quote: ... the Wizard Genomic DNA Purification Kit was used including both lysozyme and lysostaphin (10 mg/ml each) (Ref. A1120, Promega Corporation, Spain); PacBio sequencing ...
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bioRxiv - Microbiology 2020Quote: ... All PCR products were purified with an AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, USA) and quantified using a QuantiFluor™-ST (Promega, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2019Quote: Genomic DNAs were isolated from whole blood EDTA samples with a Maxwell 16 instrument using Maxwell 16 LEV Blood DNA kits according to the manufacturer’s protocol (Promega, Madison, WI, USA). These genomic DNAs were used as templates for PCR with a panel of primers that flank the highly polymorphic peptide binding domains encoded by exon 2 of class I (Mamu-A ...
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bioRxiv - Microbiology 2022Quote: ... The PCR products were extracted from a 2% agarose gel and purified using AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, USA) and quantified by QuantiFluor™-ST (Promega, USA) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: Total genomic DNA was isolated from a piece of fin or muscle tissue using a Wizard Genomic DNA Purification kit (Promega, Tokyo, Japan) or DNeasy blood and tissue kit ...
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bioRxiv - Cancer Biology 2022Quote: ... Tumor pieces were placed in homogenizer tubes containing ceramic beads along with lysis buffer from the Total RNA Purification Kit (Norgen, 37500) and RNAse inhibitors (10 μl/mL β-mercaptoethanol and 200 units/mL RNAsin Plus (Promega, N2615)) ...
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bioRxiv - Developmental Biology 2022Quote: Genomic DNA was prepared from RV free wall and HD-area pieces using the Wizard® Genomic DNA Purification Kit (Promega, France). Samples were submitted to polymerase chain reaction analysis ...
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bioRxiv - Genetics 2022Quote: ... Total RNA was extracted from 14-day-old seedlings (12-days old for the cordycepin experiments) using the Maxwell 16 LEV Plant RNA Kit and Maxwell 16 Instrument (Promega, Madison, WI). For RT-PCR and qRT-PCR ...
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bioRxiv - Evolutionary Biology 2022Quote: ... gDNA was isolated from overnight cultures grown in the respective evolution medium inoculated by single evolved colonies using Wizard Genomic DNA purification kit (Promega, Madison, Wisconsin). We performed qPCR using Promega qPCR 2x Mastermix (Promega ...
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bioRxiv - Microbiology 2023Quote: ... United Kingdom) and genomic DNA from bacterial colonies was obtained using the Promega Wizard Genomic DNA Purification Kit (Promega, Madison WI, USA). DNA concentrations were measured with Nanodrop (Thermo Scientific ...
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bioRxiv - Microbiology 2022Quote: Genomic DNA was extracted at the NCPHL from subcultures inoculated with single bacterial colonies and grown in nutrient agar (Oxoid, USA) at 37°C overnight according to the manufacturer instructions (Wizard® Genomic DNA Purification kit, Promega, UK). Genomic DNA samples (derived from 10 environmental and 250 clinical samples ...
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bioRxiv - Microbiology 2024Quote: ... and the three randomly selected variants (AMA22_1 IHCA and AMA23 IHC AVI 4 μg/mL and R AVI 8 μg/mL) was performed following the manufacturer’s instructions using the Wizard Promega kit (Promega, Madison, WI, USA). For whole genome sequencing ...
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bioRxiv - Plant Biology 2024Quote: Total RNA was extracted from roots sampled as above using the SV total RNA isolation kit system following the manufacturer’s instructions (Promega, Madison, WI, USA). cDNA was synthesized with Superscript III reverse transcriptase (Invitrogen® ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: All split luciferase biosensors were made by In-Fusion® HD Cloning kit using firefly luciferase from the pGL3-basic vector (Promega, USA) template ...
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bioRxiv - Molecular Biology 2024Quote: ... tissue homogenization was carried out by bead beating and immediately followed by RNA extraction using the Maxwell® RSC simplyRNA Tissue Kit (Promega AS1340) as per manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: RNA was isolated from islets from ∼15-week-old male mice (WT and Kcnk16 L114P (L/P)) using Maxwell® 16 LEV simplyRNA Purification Kits (Catalog no. AS1280, Promega, USA). RNA integrity was analyzed using an Agilent 2100 Bioanalyzer ...
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bioRxiv - Microbiology 2024Quote: ... Complementary DNA (cDNA) synthesis was performed with oligo-dT primers and random hexamers using the First Strand cDNA Synthesis Kit (Promega, Madison WI) following the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: Total RNA was isolated from 1×106 cryopreserved lymph node (LN) mononuclear cells (LNMC) isolated from LN biopsies using the SimplyRNA Tissue kit for the Maxwell RSC instrument (Promega, Madison, WI). RNA was then tested for both SIV gag and a cellular gene ...
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bioRxiv - Molecular Biology 2023Quote: Genomic DNA was extracted from Tetrahymena WT (SB210, with or without BrdU-labeling) and ΔAMT1 cells using Wizard® Genomic DNA Purification Kit (Promega, A1120), sheared to 3-5kb in length with Megaruptor (Diagenode Diagnostics) ...
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bioRxiv - Microbiology 2022Quote: The extent of ADCC activation induced by human γ-globulins was evaluated with the use of an ADCC Reporter Bioassay (Core Kit; Promega, G7010) and the EnSpireMultimode Plate Reader (PerkinElmer) ...
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bioRxiv - Bioengineering 2023Quote: ... We then performed first-strand cDNA synthesis from 400 ng total RNA in 20 uL reactions using Promega GoScript Reverse Transcription Kit (Promega, catalog # A5000).
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bioRxiv - Molecular Biology 2023Quote: ... DNA for PCR was isolated from 50 µl of tissue lysate using the Maxwell RSC tissue DNA Kit (Promega, Madison, WI, USA).
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bioRxiv - Cancer Biology 2023Quote: Total RNA from MB cells treated with RA for 48 h was extracted using the SV Total RNA Isolation System kit (Promega, Madison, USA), following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... Single transformed ampicillin resistant colonies were grown in LB + Ampicillin and their DNA was extracted by the Wizard® Plus SV Minipreps DNA Purification System kit (Promega, A1460). Clones with correct insertions were evaluated by Sanger sequencing (LightRun tube ...