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Citations for Promega :
7851 - 7900 of 8213 citations for QuantiChrom Indole Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... HiBit and firefly luciferase activity were measured simultaneously using a Nano-Glo HiBiT Dual-Luciferase Reporter System kit (Promega, N1630) with purified LgBiT Protein (Promega) ...
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bioRxiv - Biochemistry 2023Quote: ... PCR reaction mixtures were solved in 1% (w/v) agarose gel and purified using Wizard SV gel & PCR Clean-Up kits (Promega). Then ...
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bioRxiv - Cancer Biology 2022Quote: ... for 72 hours and cell proliferation was assayed using the CellTiter 96 Non-Radioactive Cell Proliferation kit and a GloMax Discovery plate reader (Promega). For proteomic mass spectrometry ...
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bioRxiv - Cancer Biology 2023Quote: ... All cell lines were validated by DNA fingerprinting through the University of Colorado Molecular Biology Service Center utilizing the STR DNA Profiling PowerPlex-16 HS Kit (DC2101, Promega)(Extended Data Figure 11).
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bioRxiv - Genomics 2023Quote: DNA was extracted from the agar/cell mixture from the output and control pools using a Wizard Genomic DNA Purification Kit (Promega). The manufacturer’s protocol was followed with two modifications ...
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bioRxiv - Biochemistry 2023Quote: ... U87MG cells were authenticated within a year of their use by the Johns Hopkins Genetics Resources Core Facility by short tandem repeat analysis using the PowerPlex 16 HS kit (Promega). Unless otherwise indicated ...
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bioRxiv - Microbiology 2023Quote: Genomic DNAs were purified from 600 µL of overnight bacterial culture using the Wizard HWM genomic DNA prep kit (Promega) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... Total RNA was extracted from backup samples for transcriptome analysis using Eastep® Super Total RNA Extraction Kit (Promega, USA). Reverse transcription was performed using GoScriptTM Reverse Transcription System (Promega ...
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bioRxiv - Evolutionary Biology 2023Quote: ... the bands were excised from the gel and TA cloned using the pGEM- T easy-vector II cloning kit (Promega). The standard mini-prep step was avoided by using colony PCR ...
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bioRxiv - Microbiology 2023Quote: ... Gene expression was assessed by qPCR using primers listed in the supplemental material Table S6 and the GO Taq PCR kit (Promega). Gene expression was quantified using SYBR Green (Roche ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Quality was assessed with an Agilent Bioanalyser and the quantity was calculated by an RNA-specific kit from Quantus (Promega).
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bioRxiv - Plant Biology 2023Quote: ... Transcription factors were expressed using at least 2000 ng PCR product per sample with the TnT T7 Quick for PCR DNA in vitro protein expression kit (Promega). All reaction volumes were doubled to yield a total of 100 µL protein product per transcription factor ...
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bioRxiv - Microbiology 2023Quote: High-molecular weight genomic DNA was isolated based on a modified version of Promega’s Genomic DNA Isolation Kit (Promega, A1120) [63] ...
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bioRxiv - Plant Biology 2023Quote: ... Purified DNA was run on 2% agarose gel and bands corresponding to ∼150 bp were cut and purified with a Gel Purification kit (Promega). Libraries were constructed using the Nugen Ovation Ultralow Library System V2 following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: Total RNA was extracted from frozen ground tissues using the innuPREP Plant RNA kit (Analytic Jena) and digested with RQ1 DNase (Promega). First-strand cDNA synthesis was conducted on 1 µg RNA using the SuperScript III Reverse Transcriptase (Invitrogen ...
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bioRxiv - Synthetic Biology 2023Quote: ... The Wizard™ Plus SV Minipreps DNA Purification System and Wizard™ Genomic DNA Purification Kits were purchased from Promega, U.S.A ...
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bioRxiv - Genetics 2023Quote: High molecular weight DNA was isolated from spleen tissue of a single male from each of the 11 imported Nachman wild-derived inbred strains using the Wizard DNA Purification Kit (Promega) or the Monarch HMW DNA kit (NEB ...
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bioRxiv - Microbiology 2023Quote: ... Plasmid carrying cells were cultured overnight in DM250 supplemented with 50 μg/ml kanamycin and DNA isolated using a Wizard Genomic DNA Purification Kit (Promega). Amplification of target genes was carried out using a SYBR Green based qPCR mix consisting of Q5® High-Fidelity 2× Master Mix (NEB) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... with 2% 1-thioglycerol using a Bio-Gen PRO200 homogenizer (PRO Scientific, Oxford, USA) and then extracted RNA using the Maxwell RSC simplyRNA Tissue Kit (Promega). We quantified extracts using a Quant-IT RNA Assay Kit ...
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bioRxiv - Cancer Biology 2023Quote: Genomic DNA was isolated from peripheral blood samples using the Maxwell RSC Blood DNA kit (Promega, Cat# AS1400 using the Maxwell RSC benchtop automated DNA/RNA extraction instrument (Promega). Total CER-1236 T cells present in the blood was determined using ddPCR as previously described (17) ...
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bioRxiv - Bioengineering 2023Quote: ... PCR products were confirmed on a 0.8% agarose gel and purified using Wizard® SV Gel and PCR Clean-Up kit (Promega). DNA fragments were quantified using Nanodrop (ThermoFisher Scientific) ...
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bioRxiv - Biophysics 2023Quote: ... PB-mClover3-H2B::PGK-BSD and PB-mRuby3-NPM1::PGK-Puro were co-transfected with CAG-PBase into HeLa S3 using a FUGENE HD transfection kit (Promega). Stable clones were obtained by sequential Puromycin and blasticidin selection ...
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bioRxiv - Plant Biology 2023Quote: ... Leaf samples of the high dyad producing plants were stored at -80°C before DNA extraction with the Wizard genomic DNA purification kit (Promega). A pooled DNA sample (equal mass ...
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bioRxiv - Cell Biology 2023Quote: ... and used as template to generate dsRNA in vitro using the Promega T7 RibomaxTM Express RNAi System kit (Promega, P1320). The dsRNA was precipitated with sodium acetate (300 mM final ...
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bioRxiv - Microbiology 2023Quote: ... core and modified histones and transcription factors at the HIV-1 LTR was assessed by quantitative PCR using primers spanning the full promoter (Table 1) with GoTaq qPCR Master mix kit (Promega) in a CFX Connect Real-Time PCR thermocycler (BioRad) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and used the GoScript Reverse Transcription System kit to generate cDNA from extracted RNA (Catalog #A5000, Promega Corporation, Madison, WI). We designed primers using the tool primer-Blast134 and the predicted cDNA sequence of kitlga (Fig ...
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bioRxiv - Developmental Biology 2024Quote: ... Lysates were diluted 1:10 with Milli-Q water and processed for PCR using GoTaq G2 Flexi DNA Polymerase kit (Promega). Reactions were placed in a thermocycler on a touchdown program ...
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bioRxiv - Microbiology 2024Quote: ... In vitro mRNA synthesis was then carried out at 37 °C for 4 h in T7 RiboMAX Large Scale RNA Production System kit (Promega) according to the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2024Quote: ... RNA was isolated from the cell lysis using Maxwell® RSC simply RNA Cells/Tissue Kit (Promega, Madison, WI, USA) and cDNA was synthesized ...
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bioRxiv - Developmental Biology 2024Quote: ... The 4 µg of linearized vectors were transcribed 5h using RiboMAX™ Large Scale T7 RNA polymerase kit (Promega, #P1300) with 5.6 mM Ribo m7G Cap Analog (Promega ...
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bioRxiv - Developmental Biology 2024Quote: The efficacy of all MOs was verified in western blots following in vitro transcription and translation (TNT-coupled Wheat Germ Extract kit, Promega) of pCMV-Sport6-marcks.S ...
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bioRxiv - Microbiology 2024Quote: Genomic DNA was extracted from bacterial cells grown overnight in MOKA-rich medium using the Wizard genomic DNA purification kit (Promega) or as described (62 ...
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bioRxiv - Molecular Biology 2024Quote: ... Macroscopic dissection of tumor areas was performed with sterile scalpels and tissues were extracted using the Maxwell RSC FFPE Plus DNA Purification Kit (Promega) according to the manufacturer’s instructions.
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bioRxiv - Evolutionary Biology 2024Quote: ... Bacterial genomic DNA was extracted from static overnight cultures of WS clones using the Wizard Genomic DNA purification kit (A1125, Promega) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: Stereocaulon virgatum RNA was extracted from the fungal culture using the Maxwell® RSC Plant RNA Kit (Promega, Madison, WI). Tissue was homogenized in 30 second intervals for 1.5 minutes at 30hz ...
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bioRxiv - Microbiology 2024Quote: ... Total DNA from feather pulps and tracheas were extracted using the MagaZorb® DNA mini-prep kit (Promega, Madison, WI), following the manufacturer’s recommendations with a few modiications ...
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bioRxiv - Microbiology 2024Quote: ... The bacteria were pelleted by centrifugation and DNA extraction was performed with the Wizard® genomic DNA purification kit (Promega).
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bioRxiv - Neuroscience 2021Quote: ... Culture media was then replaced with 50 µL of qNSC/aNSC media containing 1 mM 2DG (2-deoxy-D-Glucose, provided in Glucose Uptake-Glo kit from Promega (J1342)) reagent for 10 minutes in incubator (humidified ...
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bioRxiv - Cell Biology 2020Quote: ... and SERBP1 were amplified from human cDNA and cloned into the appropriate vectors (pACT or pBIND) provided by the CheckMate Mammalian Two-Hybrid kit (Promega E2440). The appropriate combination of edited pACT and pBIND vectors ...
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bioRxiv - Genetics 2021Quote: ... Approximately 1 μg RNA per sample was converted to cDNA using the ImProm-II Reverse Transcription System kit (Promega, Madison, WI) using the Oligo(dT)15 primer supplied with the kit and with a final concentration of 3 mM MgCl2 according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... the fluorescence derived from the incorporation of BRYT Green® Dye into the double-stranded PCR products was measured at the end of each cycle using the GoTaq® qPCR Master Mix 2X Kit (Promega). The results were analysed using Bio-Rad CFX Maestro software ...
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bioRxiv - Microbiology 2019Quote: ... from 25 randomly selected CM samples was extracted by an automated Maxwell 16 DNA extraction platform using blood DNA purification kits (Promega, UK) following previously described protocols2 ...
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bioRxiv - Microbiology 2019Quote: ... Viral RNA was isolated from homogenized tissues using the Maxwell 48 RSC Viral Total Nucleic Acid Purification Kit (Promega, Madison, WI) on a Maxwell 48 RSC instrument ...
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bioRxiv - Neuroscience 2021Quote: RNA was extracted from 10 flies for each experiment condition and the process followed the protocol of Promega ReliaPrepTM RNA Tissue Miniprep System kit (Promega, Z6110). Extracted RNA was stored in a −70°C refrigerator ...
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bioRxiv - Biophysics 2022Quote: ... The resulting 2.6-kb fragment was separated from the 1.4-kb fragment and uncut plasmid by agarose gel purification (Promega Gel Wizzard Kit). A set of oligonucleotides that form a biotinylated and primed fork was ligated to one end of the fragment and the final product purified on a Sepharose-4B column as previously described [42] ...
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bioRxiv - Microbiology 2022Quote: ... A total of 56 Fusobacterium isolates were recovered and subjected to DNA extraction using the Wizard genomic extraction kit (Promega, USA). For each isolate ...
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bioRxiv - Plant Biology 2019Quote: ... 1 g of fresh leaves were collected from each genotype and DNA was extracted using the Wizard Genomic DNA Purification Kit (Promega, USA) following manufacturer instructions ...
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bioRxiv - Plant Biology 2019Quote: Total of RNA was extracted from various tissue of plants by using the Eastep®Super Total RNA Extraction Kit (Promega). The cDNA was synthesized from Dnase I treated total RNA using M-MLV 1st Strand Kit (Invitrogen) ...
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bioRxiv - Synthetic Biology 2019Quote: Liquid cultures of the transformed cells were used for plasmid extraction and purification using the Wizard® Plus SV Minipreps DNA Purification System kit (Promega). The plasmids quality and concentration were quantified using the Synergy HTX plate reader (BioTek ...
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bioRxiv - Developmental Biology 2021Quote: ... pCDNA-Cnot3 Δ701-751 and pCDNA-Cnot3Δ651-751 were in vitro transcribed/translated using a TNT® Quick Coupled Transcription/Translation kit (Promega, USA) according to manufacturer’s instructions using 10μCi of 35S-Methionine to radiolabel the proteins ...