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Citations for Promega :
6801 - 6850 of 9065 citations for Total Carbohydrate Microplate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... Firefly luciferase and REN activity were measured after 48 h in extracts from infiltrated leaf discs using the Dual-Luciferase Reporter Assay System (Promega, USA) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... DDX60 (Fw 5’- AAGGTGTTCCTTGATGATCTCC-3’ Rv : 5’ -TGACAATGGGAGTTGATATTCC-3’) as analyzed by semiquantitative PCR using the SYBR Green assay GoTaq® qPCR Master Mix (Promega) with standardized primers (Metabion) ...
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bioRxiv - Immunology 2022Quote: ... Loss of cell viability reflecting the efficiency of viral infection was measured 3 days after infection by adding 100 μL Cell Titer-Glo reagent to each well (CellTiterGlo Luminescent Cell Viability Assay, Promega #G7571), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... We performed duplicate serial dilutions using supernatants collected from the virus rescues and measured luciferase expression at each dilution using Bright-Glo Luciferase Assay System (Promega, E2610). Virus titers were calculated as relative light units (RLU ...
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bioRxiv - Molecular Biology 2022Quote: ... Transfected cells were washed with 1X PBS and then lysed in 65 μL of 1X Passive Lysis Buffer (Dual-Luciferase Reporter Assay System, Promega, E1910) by shaking for 15 min at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: The metabolic activity of MEF and MEF LRP1-/- cells (cell viability) was determined by using the CellTiter-Blue® cell viability assay (Promega) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... The samples were incubated at 30 °C and luminescence was quantified after 2 h and 4 h post infection (20 μl sample plus 20 μl substrate, Nano-Glo® Luciferase Assay System, Promega). The sample was carefully removed from the upper part of the tube without disturbing the sedimented erythrocytes in the blood samples.
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bioRxiv - Neuroscience 2023Quote: Cytotoxicity was assessed by measuring the lactate dehydrogenase (LDH) release from cells with damaged membranes using the CytoTox-ONE Homogeneous Membrane Integrity assay (#7891, Promega, USA). For this ...
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bioRxiv - Neuroscience 2022Quote: ... The cerebral cortex was dissected and assayed for Luc activity as described (27) using an assay system reagent (Promega, Madison, WI) on a Lu-minoskan Ascent luminometer (Thermo Electron Corp ...
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bioRxiv - Microbiology 2022Quote: Activity of caspases-3/7 and -8 were assessed using the corresponding Caspase-Glo® Assays in white-walled 96-well plates (Promega).
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bioRxiv - Bioengineering 2022Quote: Cytochrome CYP3A4 enzymatic activity of HLCs was measured using P450-Glo™ Assay with Luciferin-IPA (catalogue no. V9001, Promega, Sweden). The relative luminescence was normalized to total protein content ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell viability was quantified by detection of ATP content in the culture medium with CellTiter-Glo® Luminescent Cell Viability Assay (Promega) by following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... The firefly luciferase activity was determined 24 h later with the Bright-Glo™ Luciferase Assay System (Promega, Madison, WI, USA) on the Envision plate reader (PerkinElmer ...
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bioRxiv - Cancer Biology 2024Quote: The apoptotic effect of MK-1775 was determined by means of caspase 3/7 activity via Apotox-Glo Triplex Assay (Promega, #G6320) according to the manufacturer’s protocol ...
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... Arc-Hibit signal was identified by complementation with LgBiT (equal volumes) and measurement of Nanoluc-mediated luciferase [JDS1] (Nano-Glo Luciferase Assay, Promega, 565nm) on a microplate reader (SpectraMax iD3).Total Arc in the cell lysate is calculated by dividing the luciferase value of Arc cell lysate by the % of Arc lysate sample used from total sample collected ...
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bioRxiv - Physiology 2024Quote: ... The toxicity of MNP was determined 24 h upon MNP treatment using the LDH-toxicity Assay (Promega Corp, Fitchburgh, WI, US) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: The direct antiviral effect of betacyanin fractions was evaluated through the formation of viral CPE using the Viral ToxGloTM Assay (Promega, USA) per the manufacturer’s instructions with slight modifications ...
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bioRxiv - Genetics 2024Quote: Cell viability in the presence of increasing levels of NaPr and NaBu was performed using the CellTiter-Blue® Cell Viability Assay (Promega). TSA was used as a negative control ...
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bioRxiv - Immunology 2024Quote: ... Quantification of SARS-CoV-2 RVP infection was determined using the Renilla-Glo® luciferase assay system (Promega Corp., Madison, WI). Microtiter assay plates were centrifuged for 5 min at 2000 rpm to prevent cell loss ...
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bioRxiv - Microbiology 2024Quote: ... or 4 μg of the pNL4.3-ΔEnv EGFP backbone36 (for viral entry assay in primary CD4+ T cells) into 293T cells using the FuGENE6 transfection reagent (Promega, USA). The cells were cultured at 37°C for 6 hours before the medium was completely replaced with fresh medium ...
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bioRxiv - Immunology 2024Quote: ... The virus neutralization titers were determined by measuring the NLuc activity using the Nano-Glo Luciferase Assay System (Promega, Madison, WI) following the manufacturer’s conditions ...
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bioRxiv - Microbiology 2023Quote: ... for 2 hours at 72 hours and lysed at 98 hours using 25 μl Dual-Glo® Luciferase Assay System (Promega). Luminescence was measured ...
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bioRxiv - Cell Biology 2023Quote: The activity of the proteasome’s β5 sites was determined either by Succinyl(Suc)-LLVY-AMC (7-amido-4-methylcoumarin) fluorogenic substrate or by the Proteasome-Glo™ assay (Promega), a luciferase coupled assay ...
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bioRxiv - Cancer Biology 2024Quote: ... cell viability was evaluated by measuring either luminescence or absorbance (490 nm) with the CellTiter-Glo® luminescent cell viability assay (Promega) or the CellTiter (Promega) ...
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bioRxiv - Microbiology 2023Quote: ... and cellular expression of the luciferase reporter indicating viral infection was determined using the ONE-Glo™ Luciferase Assay System (Promega) 48 h after infection ...
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bioRxiv - Cell Biology 2022Quote: After extraction of proteins (see above) without proteinase and phosphatase inhibitors the Src activity was evaluated with ProFluor® Src-Family Kinase Assay (Promega).
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bioRxiv - Microbiology 2023Quote: ... The migrated cells in the lower chamber were stained with 5 μl of CellTiter 96 AQueous One Solution Cell Proliferation Assay (Promega # G3580) for 2.5 h at 37° C with 5% CO2 ...
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bioRxiv - Molecular Biology 2022Quote: ... Viability of PBMC following exposure to test items was assessed by resazurin reduction assay (CellTiter-Blue Reagent, Promega, Madison, WI, USA). Cytotoxicity was assessed by measurement of lactate dehydrogenase in the cell culture supernatant (CyQUANT™ LDH Cytotoxicity Assay ...
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bioRxiv - Neuroscience 2023Quote: ... 10µl of luciferase containing culture medium was transferred into a fresh 96 well plate and incubated with either Furimazine (Nano-Glo™ Luciferase Assay, Promega)/Coelenterazine (Prolume ...
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bioRxiv - Cancer Biology 2023Quote: ... The firefly and renilla luciferase activity assessment were conducted 48h post-transfection using the Dual-Glo® Luciferase Assay System (Promega), according to the manufacturer’s protocol and the GloMax® Discover Microplate Reader (Promega).
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bioRxiv - Biochemistry 2023Quote: ... nLuc luciferase signal was measured by mixing 25 μL of Nano-Glo Luciferase Assay System (prepared 1:50 as recommended; Promega # N1120) with 25 μL of diluted reactions (diluted 1:5 in Glo Lysis Buffer ...
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bioRxiv - Biochemistry 2023Quote: ... 25 μL diluted lysate was mixed with an equal volume of Renilla-Glo Luciferase Assay System (prepared 1:100 as recommended; Promega # E2710) for 10 min at room temperature.
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bioRxiv - Cancer Biology 2023Quote: ... Cell viability of (non-)treated tumor spheroids was evaluated using the CellTiter-Glo 3D Cell Viability Assay (Promega, Fitchburg, WI, USA) according to the manufacturer’s instructions with slight modifications ...
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bioRxiv - Molecular Biology 2023Quote: ... Produced pseudoviruses were titrated on HEK-293T-ACE2 by performing duplicate serial dilutions and virus titers were measured 48 hours after infection using Bright-Glo Luciferase Assay System (Promega, E2610).
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bioRxiv - Microbiology 2023Quote: ... The presence of glycosylated proteins in the cell lysates was assessed using Promega Endoglycosidase H (Endo H) assay reagents (Promega V4871). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... The amount of pseudoviruses prepared was quantified by the HiBiT assay using Nano Glo HiBiT lytic detection system (Promega,Cat# N3040) as previously described.1–5 ...
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bioRxiv - Evolutionary Biology 2023Quote: Target gene and AAEL002401(reference gene) expression was quantified by RT-qPCR using FAM- and HEX -labelled GoTaq® Probe qPCR and RT-qPCR assay (Promega), TaqMan universal PCR master mix (Promega ...
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bioRxiv - Microbiology 2023Quote: The cytotoxic activity on Hela cells of each bacterial strain was analyzed using the CellTiter-Blue® Cell Viability Assay (Promega) following manufacturer instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... and 1:2000 RealTime-Glo MT Cell Viability Assay Substrate (to visualise Nluc, first diluted 1:1 in DMSO, Promega G9711).
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bioRxiv - Microbiology 2023Quote: ... LgBit and SmBiT complementation generated luminescent signal was measured in live cells using Nano-Glo® Live Cell Assay System (#N2011, Promega) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: Cell viability was determined at DIV 10 using the CellTiter-Blue Cell Viability Assay according to the manufacturer’s instructions (ref: 8080, Promega, Madison, WI). In brief ...
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bioRxiv - Synthetic Biology 2023Quote: ... 10 μL of the dilution was mixed with 20 μL of passive buffer which was then mixed with 30 μL ONE-Glo™ EX Luciferase Assay Reagent (Promega) and incubated at room temperature for 10 min ...
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bioRxiv - Cancer Biology 2023Quote: ... cell viability was assessed using the MTS assay (CellTiter 96® AQueous One Solution Cell Proliferation, G3580, Promega, Madison, WI, USA). In brief ...
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bioRxiv - Cancer Biology 2023Quote: FOXM1 3’UTR region comprising of miRNA binding region was cloned into pmirGLO-Dual Luciferase miRNA target expression vector for miRNA luciferase reporter assay (Promega, USA) in MCF-7 cell lines ...
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bioRxiv - Genomics 2023Quote: ... Each culture was then sampled by mixing 80uL of culture with 80uL of DI water and 80uL of Nano-Glo Luciferase Assay System reagent according to the manufacturer’s instructions (Promega, Madison, WI). Luminescence signals were measured using a BioTek Synergy H1 microplate reader and then normalized using OD600 measurements as a proxy for cell density.
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bioRxiv - Immunology 2023Quote: FACS-purified T cell subsets were incubated overnight with a TGF-β reporter cell line28 and luciferase activity detected via the Luciferase Assay System (Promega). TGF-β activity was determined as previously described16.
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bioRxiv - Microbiology 2023Quote: The effect of pyocin SX2 on protein synthesis was assessed by an in vitro transcription-translation assay (E. coli T7 S30 Extract System for Circular DNA. Promega, Germany). pRL-SV40 vector (Promega ...
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bioRxiv - Microbiology 2023Quote: ... The quantity of the reporter protein was examined by measuring its enzymatic activity through the Renilla Luciferase Assay System (Promega, Germany), as per manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2023Quote: ... Reactions were incubated for 1 hr at 30 °C and luciferase signal was measured using Dual-Glo Luciferase Assay System (Promega E2920).
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bioRxiv - Immunology 2023Quote: ... the Bio-GloTM luciferase assay system was applied to estimate the T-cell activation according to the manual (Promega, Madison, WI). The relative luminescence unit (RLU ...