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Citations for Promega :
6651 - 6700 of 9065 citations for Total Carbohydrate Microplate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Cells were left to grow for 48 hrs of treatment (unless indicated otherwise) and then cytotoxicity was measured using the CellTiter-Glo Luminescent Cell Viability Assay (Promega) according to manufacturer protocol with a Molecular Devices Spectramax M3 plate reader.
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bioRxiv - Genomics 2024Quote: ... Renilla luciferin reagent (25 μl of Stop & Glo Reagent, Promega Dual-Luciferase Reporter Assay System; Promega, Madison, WI, cat. #E1910) was injected into each well through one of the injectors of a CentroXS3 instrument (Berthold Technologies) ...
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bioRxiv - Genetics 2024Quote: ... HEK293T cells cultured in a 12-well plate were harvested and processed according to the instruction of Dual-Glo Luciferase Assay System (Promega) with minor modification ...
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bioRxiv - Synthetic Biology 2024Quote: ... and then transferred to 96-well plates (white with a clear flat bottom) containing Nano-Glo Luciferase Assay Reagent (Promega) diluted 1000-fold in PBS at room temperature ...
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bioRxiv - Synthetic Biology 2024Quote: ... Bioluminescence measurements were taken by combining 25 µl of the sample solution with an equal volume of prepared buffer-reconstituted nluc substrate as detailed by the manufacturer (Nano-Glo Luciferase Assay System; Promega). Measurements were taken every 10 min in Nunc™ F96 MicroWell™ 446 plates using a GloMax® navigator luminometer (Promega ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Plates were then incubated for 72 h at 37°C and 5% CO2 and cell viability determined by CellTiter-Glo Luminescent Cell Viability Assay (Promega). In short ...
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bioRxiv - Immunology 2024Quote: ... The supernatants were used to determine lactate dehydrogenase activity and IL-1β level using LDH-GloTM Cytotoxicity Assay (Promega, J2380) and Mouse IL-1 beta DuoSet ELISA (R&D Systems ...
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bioRxiv - Plant Biology 2024Quote: ... benthamiana leaves were harvest after 48 hr of agroinfiltration99 and luciferase activity was quantified using the dual-luciferase reporter assay system (Promega). To determine the relative promoter activity ...
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bioRxiv - Microbiology 2024Quote: ... Pseudovirus titers were determined by infecting TZM-bl-luciferase reporter cell lines and estimating 50% tissue culture infectious dose (TCID50) using the Bright-Glo luminescence reporter gene assay system (Promega)73 as directed by the manufacturer.
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bioRxiv - Genetics 2022Quote: Genomic DNA was isolated from blood samples using commercial kit (Wizard® Genomic DNA Purification Kit; Promega), following the fabricant protocol ...
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bioRxiv - Plant Biology 2020Quote: ... corniculatus plants was synthesized from 3 μg of total RNA using a Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV-RT) (Promega, WI, USA) and 100 pmol of random hexamers (Pharmacia Biotech) ...
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bioRxiv - Molecular Biology 2020Quote: ... qRT-PCR was executed in a total of 20μl reaction volume which includes 2 X Mix SYBR green I (10μl; Promega Corporation, Madison, WI, USA), primer (0.25μl ...
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bioRxiv - Neuroscience 2021Quote: ... 3 biological replicas were collected to extract the total RNA and the cDNA was synthetized using GoScript™ Reverse Transcription Mix (Promega A2790). Primer pairs used for the qPCR are listed in Table S10.
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bioRxiv - Molecular Biology 2019Quote: ... Two micrograms of total RNA were reverse transcribed into the first strand cDNA using the ImProm-II Reverse Transcription System (A3800, Promega, Madison, USA) according to the manufacturer’s protocol and used as a template for the amplification of the full-length camel IFNε cDNA ...
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bioRxiv - Neuroscience 2021Quote: ... One microgram of total RNA was transcribed to cDNA by reverse transcriptase enzyme action Improm Pre-II ® (Promega, Madison, Wisconsin, USA). Quantitative RT-PCR reaction in real time was done on an ABI Prism ® 7500 Sequence Detection System (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2021Quote: ... IPs were performed by incubating lysates equivalent to 20 mg total protein supplemented with RNase inhibitor - RNasin (Promega, Germany, Cat. No. N2511) and 1 mM DTT with 80 μl of anti-GFP magnetic beads GFP-Trap (Chromotek ...
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bioRxiv - Cell Biology 2020Quote: ... The cDNA synthesis was performed from 2.5 μg of total RNA and oligo-dT and MMLV reverse transcriptase (Promega, Madison, WI, USA) according to supplier instructions ...
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bioRxiv - Synthetic Biology 2020Quote: ... was added to 20 μL of each sample and total luminescence over 5s (expressed in relative light units, RLU) was measured using a Glomax 20/20 luminometer (Promega, WI, USA). Separately seeded cells were transfected with pCMV-GLuc2 plasmids (1 μg ...
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bioRxiv - Biochemistry 2019Quote: ... A total of 100 µg of each protein sample was digested with trypsin (Trypsin Gold, Mass Spec Grade, Promega, Fitchburg, Wisconsin, USA) by using filter-aided sample preparation (FASP ...
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bioRxiv - Cell Biology 2020Quote: ... Total 500 ng RNA was used to synthesize cDNA by using Moloney-murine leukemia virus (M-MLV) reverse transcriptase (Promega, Madison, WI). Diluted cDNA was used in each quantitative reverse transcription-PCR (qRT-PCR) ...
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bioRxiv - Biophysics 2020Quote: ... First-strand cDNA was synthesized from 2 μg of total RNA with oligo (dT)15 primers and GoScript™ Reverse Transcriptase (Promega, Switzerland) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... A total of 200 ng total RNA was used for reverse transcription reactions to synthesize cDNA with revere transcription system A3500 (Promega, Madison, WI). Then ...
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bioRxiv - Neuroscience 2022Quote: ... was synthesized from 1.0 µg of total RNA using MMLV reverse transcriptase and a cocktail of oligo dT and random primers (Promega, Madison, WI, USA). qPCR was performed using PowerSYBR green PCR master mix (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: Total RNA of TH3o and O23 was extracted at different stages (24 and 48 h) of barley infection using the SV Total RNA Isolation System (Promega, WI, USA). One microgram of total RNA was used to prepare each sequencing library with the NEBNext Ultra II Directional RNA library prep kit (New England Biolabs Japan ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA synthesis was performed using 1 µg of total RNA in combination with oligo(dT) and Improm-II reverse transcriptase (Promega, Madison, WI). qPCR analysis was performed as described previously [55] with SYBR green PCR master mix (Applied Biosystems ...
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bioRxiv - Neuroscience 2023Quote: ... A fragment of the rat Piezo 1 cDNA was amplified with Taq Polymerase and the oligonucleotide primers 5’-GAGGAAGAGGACTACCTT and 5’-TTTACTTAGAAAACCCTACAG from bladder total RNA and cloned into the pGEM-T Easy vector (Promega, Madison, WI). Sequence was confirmed by Sanger capillary sequencing and sense and antisense RNA probes were synthesized with T7 and SP6 RNA polymerases (Roche-Sigma-Aldrich) ...
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bioRxiv - Genomics 2023Quote: ... traces of genomic DNA were removed by treating 600 ng of total RNA with 1 µl RQ1 RNase-Free DNase (Promega, cat# M6101) in 9 µl reaction additionally containing RQ1 DNase buffer and 1 µl of murine RNase inhibitor at 37°C for 45 minutes ...
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bioRxiv - Plant Biology 2023Quote: ... and DR1-107 lines using the SV total RNA Isolation System that includes on-column DNase treatment (Promega, Inc., Madison, WI, USA). The quality of RNAs was assessed on agarose gel and NanoDrop microvolume spectrophotometer (Thermo Scientific ...
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bioRxiv - Microbiology 2023Quote: Total nucleic acid extraction from swab and tissue samples was performed using ReliaPrep™ Viral TNA Miniprep System (Promega, Madison, WI, USA). Swab samples were simply inverted in the tube and briefly centrifuged prior to extraction ...
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bioRxiv - Molecular Biology 2024Quote: ... total RNA was extracted from BHK-21 cells transfected with viral genomic RNAs by using the SV Total RNA Isolation System (Promega, Madison, WI) based on the user’s manual ...
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bioRxiv - Cancer Biology 2021Quote: ... The endpoints of the second screen were caspase 3/7 activity measured using the CaspaseGlo® 3/7 assay reagent (Promega) at the 8-hour and 16-hour time intervals ...
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bioRxiv - Neuroscience 2021Quote: Cell cytotoxicity was detected by measuring lactate dehydrogenase (LDH) levels using the CytoTox 96® Non-Radioactive Cytotoxicity Assay (Promega, G1780). The manufacturer’s instructions were followed and the absorbance reflecting the LDH content in the cell supernatant was measured at 492 nm (600 nm reference ...
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bioRxiv - Cancer Biology 2021Quote: ... were seeded in 96-wells white plate and caspase-1 activities were assessed using Caspase-Glo 1 Inflammasome Assay (Promega #G9951) according to manufacturer’s instructions.
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bioRxiv - Cancer Biology 2021Quote: Intracellular NanoBRET assays were performed as previously described (10) using NanoLuc®-STK3 Fusion Vector Cat.# NV4301 (Promega, Madison, WI, USA). Briefly ...
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bioRxiv - Cell Biology 2020Quote: ... Cell growth and viability were measured on the 96 well white bottom plate as an end point measurement for each time point and/or treatment using the Cell-titer GLO assay (Promega, # G7570) based on manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... The caspase-3/7 activity of the lysates or the plated cells was analysed using Apo-ONE ® Homogenous Caspase-3/7 Assay (Promega) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... TUNEL assay was performed using a DeadEnd(tm) Fluorometric TUNEL System according to the manufacturer’s instructions (Promega Corporation, Madison, WI, USA). Briefly ...
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bioRxiv - Molecular Biology 2021Quote: ... The activities of firefly luciferase (LUC) and Renilla luciferase (REN) were measured using a Dual-Luciferase Reporter Assay System (E2920, Promega, USA). The LUC activity was normalized to the REN activity ...
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bioRxiv - Neuroscience 2020Quote: ... cells were washed with PBS and lysed directly on the plate on ice using the Cell Culture Lysis Reagent from the Luciferase Assay System (Promega#E1500). Lysates were transferred to microcentrifuge tubes ...
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bioRxiv - Developmental Biology 2020Quote: ... and 20 µL of resulting supernatant was used to measure luciferase activity with the Dual-Luciferase Reporter Assay System (Promega, E1980) on a GloMax-Multi plate reader (Promega).
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bioRxiv - Microbiology 2022Quote: ... medium was changed and cellular expression of luciferase reporter indicating viral infection was determined using ONE-Glo™ Luciferase Assay System (Promega) in 48 hr after infection ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Cell viability was determined prior to gene expression studies on day 7 by measuring ATP release in supernatants with the CellTiter-Glo 3D assay (Promega, G9681) according to the manufacturer’s protocol to pre-specify appropriate testing ranges ...
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bioRxiv - Neuroscience 2021Quote: ... neonatal and adult astrocytes plated on 96-well plates (50,000 cells per well) was measured with the Glucose Uptake-Glo™ Assay (Promega, J1341) according to manufacturer’s protocol ...
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A human cancer cell line initiates DNA replication normally in the absence of ORC5 and ORC2 proteinsbioRxiv - Molecular Biology 2020Quote: ... The absorbance of cells was measured every 24 hours using CellTiter 96® Non-Radioactive Cell Proliferation Assay (Promega, Madison, WI) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... 25 μL of cell lysate was distributed to mix with 25 μL of Nano-Glo Luciferase Assay System (Promega, Cat.# N1120), and another 25 μL of cell lysate was mixed with 25 μL of ONE-Glo Luciferase Assay System (Promega ...
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bioRxiv - Molecular Biology 2020Quote: ... and then Renilla luciferase activities were measured after the addition of 100 ml of Stop & Glo® Reagent (Luciferase Assay Reagent, Promega). Finally ...
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bioRxiv - Genomics 2020Quote: ... DNA was subcloned into the enhancer barrier vector28 (gift from Dr. Laura Elnitski, NHGRI) or pGL4.23 for enhancer assays (Promega, Madison, WI). Constructs were prepared using the Plasmid MidiPrep Kit (Qiagen ...
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bioRxiv - Molecular Biology 2021Quote: ... Firefly luciferase activity was measured by adding 25 μL Luciferase Assay Buffer II (LARII) to 5 μL of lysate and measured immediately with the GloMax 20/20 Luminometer (Promega, E5311). Reactions were stopped and Renilla luciferase activity was measured by adding 25 μL Stop&Go reagent ...
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Efficient Suppression of Endogenous CFTR Nonsense Mutations Using Anticodon Engineered Transfer RNAsbioRxiv - Molecular Biology 2021Quote: PTC readthrough by ACE-tRNA was assessed by measuring NanoLuc (NLuc) luciferase activity using the Nano-Glo Luciferase Assay System (Promega, #N1130) on a Synergy2 multi-mode microplate reader (BioTek Instruments ...
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bioRxiv - Molecular Biology 2021Quote: ... Lysates that produced over 104 luminescence units sing a high expression RNA containing NanoLuc Luciferase (A[WT]) and the Promega Nano-Glo Luciferase assay system (Promega N1110) in preliminary tests were pooled and optimized for the needed amount of Mg(OAc)2 and incubation time using A[WT] mRNA ...