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Citations for Promega :
6701 - 6750 of 9065 citations for Total Carbohydrate Microplate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... The experiments performed here used a pool of lysates from multiple different4 harvests that were each individually tested for a minimal activity of 104 using a high expression RNA containing NanoLuc (A[WT]) and the Promega Nano-Glo Luciferase assay system (Promega N1110). Pooled lysates were then optimized for the needed amount of Mg(OAc)2 and an optimal incubation time at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... 8uL of reaction was mixed with 8uL of Nano-Glo buffer/substrate mix following the Nano-Glo Luciferase Assay System (Promega N1110) instructions ...
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bioRxiv - Developmental Biology 2019Quote: ... Cell lysates were harvested the day after and luciferase and β-galactosidase activities were measured using Luciferase Reporter and Beta-Glo Assay Systems (Promega) following the manufacturer’s guidelines ...
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bioRxiv - Cancer Biology 2019Quote: ... The cells were incubated for 4 days and cell viabilty was measured using CellTiter-Glo® Luminescent Cell Viability Assay (Promega).
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bioRxiv - Physiology 2019Quote: ... Samples were mixed with the same amount of substrate provided by the One Glo Luciferase Assay System (Promega, Mannheim, Germany, #E6110) immediately before signal detection ...
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Characterization of GRK5 as a novel regulator of rhabdomyosarcoma tumor cell growth and self-renewalbioRxiv - Cancer Biology 2019Quote: RMS cell growth was assessed via cell counts or an ATP-based luminescent cell viability assay,CellTiter-Glo (Promega, Madison, WI). Myogenic differentiation was performed following serum starvation of RMS cells in 2% horse serum/DMEM for 72 hours prior to fixation in 2% paraformaldehyde ...
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bioRxiv - Cancer Biology 2019Quote: ... Cell cytotoxicity in the drug-treated 2D cell cultures was assessed using the CellTiter96® AQueous One Solution Cell Proliferation Assay (Promega). Briefly ...
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bioRxiv - Microbiology 2019Quote: ... the cell viability of each infected virus variants was measured using CellTiter 96 Aqueous One solution Cell Proliferation Assay (Promega, USA). The relative cell viability was calculated by normalizing the absorbance value of treated virus samples against untreated virus samples ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Cell viability of LC5-RIC cultures exposed to HIV inoculum and test compounds was determined by performing a CellTiter-Blue® cell viability assay (Promega) and monitoring the ability of metabolically active cells to convert the redox dye resazurin into the fluorescent product resorufin ...
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bioRxiv - Molecular Biology 2020Quote: ... CA) already containing 20uL of room temperature 1X Passive Lysis Buffer from the Dual Luciferase Reporter Assay System (Promega, Madison, WI). Cultures were then lysed by shaking at 300 rpm for 25 minutes at room temperature ...
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bioRxiv - Genetics 2020Quote: ... Cells were harvested 48 hours after transfection and assayed for luciferase activity using the Dual-Luciferase Reporter (DLR™) Assay System (Promega). Data were expressed relative to renilla luciferase activity to normalize for transfection efficiency ...
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bioRxiv - Biophysics 2021Quote: ... and the twitchin kinase domain lacking flanking tails (Kin) was measured by monitoring ATP hydrolysis using the Kinase Glo™ luminescent assay system (Promega). Assays were performed using 10 µg of protein sample in a 50 µL reaction volume ...
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bioRxiv - Microbiology 2020Quote: ... Plates were incubated at 37°C (5% CO2) for 48 h before performing CellTiter-Glo (CTG) assays as per the manufacturer’s instruction (Promega, Madison, WI). Luminescence was read on a BioTek Synergy HTX plate reader (BioTek Instruments Inc. ...
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bioRxiv - Cancer Biology 2020Quote: ... expression of the luciferase reporter plasmid followed by measurement of luciferase activity after lysing cells with Bright-Glo Luciferase Assay System (Promega #E2610), both using Biotek Cytation 5 plate reader ...
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bioRxiv - Biochemistry 2020Quote: ... Cellular debris was then pelleted by centrifugation and either 5 μl or 10 μl of the clarified supernatant used in dual luciferase assays following the manufacturer’s prescribed protocol (Promega, cat # E1910). Data are shown as the ratio of RLUC to FLUC activity averaged over six experiments.
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bioRxiv - Neuroscience 2020Quote: 50 μl supernatant were collected as designed time point into a 96-well plate for lactate dehydrogenase (LDH) release assay as manufacture’s protocol (Promega, cat# G1780). Briefly ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were collected in lysis buffer and Firefly/Renilla luciferase activity were measured according to the manufacturers’ protocol using the Dual-Luciferase® Reporter Assay System (E1910; Promega) on a Microplate Luminometer LB 96V (EG&G Berthold) ...
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bioRxiv - Cancer Biology 2021Quote: ... Firefly and Renilla luciferase activities were determined 24 hours post transfection with the dual-luciferase reporter assay system (Promega, cat.no. E1910). Firefly luciferase readings were normalized against internal control Renilla luciferase and calculated as fold differences against the activity obtained from cells transfected with empty vector.
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bioRxiv - Cancer Biology 2021Quote: ... treated with LD50 concentrations of 10058-F4 or CB-839 for 12 h in DMEM without FBS then analyzed by Glutamate-Glo™ Assay (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... were assayed in white 96 well plates in triplicate by the addition of 50 μl Bright-Glo™ Firefly luciferase assay system (Promega) or freshly prepared 40 ng/ml Coelenterazine in Renilla-Glo buffer (Promega) ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell proliferation and viability were measured at 72 hours post treatment using the CellTiter Blue Viability Assay (Promega, Fitchburg, WI, USA) as described previously (24) ...
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bioRxiv - Cell Biology 2021Quote: ... bright field images of all plates were acquired with a high-throughput high-content imaging system (Operetta, Perkin-Elmer) and CellTiter-Glo luminescent cell viability assay (Promega, G7570) was performed ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... transduction efficiency was quantified by measuring the activity of firefly luciferase in cell lysates using a luciferase assay system (Promega, USA) and SpectraMax iD5 Multi-Mode Microplate Reader (Molecular Devices ...
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bioRxiv - Synthetic Biology 2021Quote: HEK cells were transfected as previously described for the SRE reporter assay with the exception of substituting the SRE reporter with CRE-dependent firefly luciferase 60 (Promega; E8471).
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bioRxiv - Microbiology 2020Quote: ... or human IFN-β (1000 U/ml) and firefly luciferase activity was determined using the dual luciferase reporter assay system (Promega) on Cytation 3 (BioTek ...
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bioRxiv - Microbiology 2020Quote: ... pseudovirus titres were measured by luciferase activity in relative light units (RLUs) (Bright-Glo Luciferase Assay System, Promega Biosciences, California, USA). Neutralization assays were performed by adding 100 TCID50 (median tissue culture infectious dose ...
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bioRxiv - Microbiology 2021Quote: ... The migrated cells in the lower chamber were stained with 5 μl of CellTiter 96 AQueous One Solution Cell Proliferation Assay (Promega # G3580) for 2 h at 37° C with 5% CO2 ...
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bioRxiv - Cancer Biology 2019Quote: Proliferation of cancer cells isolated from MMTV-PyMT;Ccr2+/+ and MMTV-PyMT;Ccr2−/− mice was measured using the CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS; Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... At the indicated time points firefly and Renilla luciferase activities were measured using the Dual-Glo® Luciferase assay system (Promega) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... Nanoluciferase activity was measured at 24 or 48 hours post-transfection (hpt) using the Nano-Glo® Luciferase Assay System (#1120, Promega) and a Berthold Centro XS3 luminometer (integration time 1s/well using the MikroWin® software) ...
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bioRxiv - Developmental Biology 2020Quote: ... Four hours after transfection the tracheal mesenchyme was induced using day5 medium in presence or absence of Wnt agonist (3μM CHIR99021) and cells were cultured for 24 hours and then lysed and assayed using Dual-Luciferase Reporter Assay System (Promega, E1980).
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bioRxiv - Microbiology 2021Quote: ... Cell viability was measured 48h later (or otherwise indicated) using CellTiter 96® AQueous One Solution Cell Proliferation Assay (G3582, Promega) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... cells were lysed and activity of firefly and renilla luciferase was measured with a Dual-Luciferase Reporter Assay System (Promega, # E1910).
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bioRxiv - Microbiology 2021Quote: ... Cells were infected at a MOI of 0.2 as determined by preliminary experiments to generate an ideal dynamic range of the colorimetric CytoTox 96 ® Non-Radioactive Cytotoxicity Assay (Promega). Infections progressed for 72 h at which time supernatant from treatments and controls were processed for LDH release according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2020Quote: ... the culture media were harvested and Nanoluc activity was determined in 10 µl aliquots of culture medium using a Nano-Glo™ Luciferase Assay System (Promega). To normalize the electroporation efficiency ...
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bioRxiv - Microbiology 2020Quote: ... and Renilla luciferase (Rluc) and firefly luciferase (Fluc) activities were evaluated using the Dual-Luciferase Reporter (DLR™) Assay System (Promega).
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bioRxiv - Cell Biology 2021Quote: Apoptotic cell death was measured in a 96-well format using the RealTime-Glo™ Annexin V Apoptosis and Necrosis Assay (Promega) per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... The cell proliferation was assessed 5 days after treatment by measuring viable cells in culture based on quantitation of the ATP present using CellTiter-Glo® Luminescent Cell Viability Assay (Promega) according to the manufacturer’s instructions using a Victor X5 Multi-label plate reader luminescence protocol (Perkin Elmer ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were then cultured overnight before measurement of luciferase and renilla reporter signals using a Dual-Luciferase® Reporter assay system (Promega) and a GloMax® Discover Microplate reader (Promega ...
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bioRxiv - Physiology 2022Quote: ... the cells were washed with PBS and harvested to determine firefly and Renilla luciferase activities according to the Dual-Luciferase reporter assay system (Promega, USA) on a luminometer (GloMax 20/20 ...
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bioRxiv - Genomics 2022Quote: ... F-luciferase and R-luciferase expression was assayed in a TECAN Spark plate reader using the Dual-Glo® Luciferase Assay System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: ... Twenty-four hours post treatment the cells were lysed and analyzed for luminescence using the Luciferase Assay system (Promega; Cat #E1500). Normalization among different technical replicates and conditions was carried out by normalizing the relative luminescence units (RLUs ...
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bioRxiv - Molecular Biology 2022Quote: BD BioCoat Poly-D-Lysine 96-well plates with black sides and clear bottoms were used to run the ApoTox-Glo™ Triplex Assay (Promega). Cells were trypsinized and removed from their culture flasks and diluted to 24,000 cells/ml and 100µl of each sample was added to each well ...
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bioRxiv - Microbiology 2022Quote: ... Cells were transferred back to the incubator and luciferase was measured at 24 hours post-infection (Bright-Glo™ Luciferase Assay System, Promega). Values were normalized to those derived from wells with pseudovirus but without sera (100% infection) ...
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bioRxiv - Microbiology 2022Quote: ... Lysates were used to measure the activity of F-Luc and R-Luc via the Dual-Luciferase Reporter Assay System (Promega, E1960) in a GloMax 20/20 luminometer (Promega ...
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bioRxiv - Molecular Biology 2022Quote: Luciferases were assayed by their activity in oxidizing luciferin with emission of luminescence using the Dual-Luciferase Reporter Assay System (Promega, USA) according to the producer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... 48 h later assays were then lysed and read on a FLUOstar Omega plate reader (BMF Labtech) using the Steady-Glo® luciferase assay system (Promega)
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bioRxiv - Immunology 2022Quote: ... centrifuged at 1,500 × g for 10 min and the viral titers were measured by luciferase activity in relative light units (RLU) (Bright-Glo Luciferase Assay System, Promega, E2620). The supernatants are aliquoted and stored at -80°C until further use ...
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bioRxiv - Immunology 2022Quote: ... The IC50 values were calculated based on the reduction of 50% relative light units (Bright-Glo Luciferase Assay Vector System, Promega, USA) compared to the virus-only control ...
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bioRxiv - Microbiology 2021Quote: ... and nanoluciferase activity was measured using Tristar multiwell Luminometer (Berthold Technology) for 2.5 seconds by adding 20 µl of Nano-Glo® substrate in nanoluc assay buffer (Promega Inc). An uninfected monolayer of Vero E6 cells treated identically served as controls for determining background and obtain normalized relative light units ...