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Citations for Promega :
6951 - 7000 of 9065 citations for Total Carbohydrate Microplate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... 10 μL of lysate was mixed with the appropriate luciferase assay substrate and light emission was measured by using a Glomax 20/20 Luminometer (Promega, Madison, USA).
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bioRxiv - Microbiology 2021Quote: ... The antibody-virus inoculum was replaced with fresh culture medium eight hours post-infection and cells processed 48 h post-infection with Nano-Glo luciferase assay system (Promega, Madison, WI). Luminescence was measured using an Enspire plate reader (PerkinElmer ...
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bioRxiv - Microbiology 2020Quote: ... the cells were harvested and lysed in reporter lysis buffer, and luciferase activity (relative luminescence unit, RLU) was measured using luciferase assay reagents and a luminescence counter (Promega, Madison, WI). Percent inhibition of serum antibodies compared to the level of the virus control subtracted from that of the cell control was calculated ...
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bioRxiv - Microbiology 2020Quote: ... 10 ul of the cell extract was mixed with the appropriate luciferase assay substrate and light emission was measured by using a Glomax 20/20 Luminometer (Promega, Madison, USA).
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bioRxiv - Microbiology 2019Quote: Luciferase assays to track transfection progress and to measure aTc-dependent regulation of a firefly reporter were performed as previously described (Ganesan et al., 2016; Wagner et al., 2013) using the Dual-Luciferase Reporter® Assay System (Promega, E1910), Renilla Luciferase Assay System (Promega ...
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bioRxiv - Cell Biology 2021Quote: ... Caspase 3/7 activity was measured 24 hours post-cytokine treatment using the Caspase-Glo® 3/7 Assay System (Promega, #G8090).
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bioRxiv - Cell Biology 2021Quote: ... Cellular viability/toxicity of the THP-1 derived macrophage system was established with the MTS cellular proliferation-viability assay (Promega, Cat#G3582).
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bioRxiv - Immunology 2022Quote: ... Pseudovirus titers were measured by infecting 293T-ACE2.TMPRSS2s cells for 48 h prior to measuring luciferase activity (luciferase assay reagent, Promega, Madison, WI). For neutralization assays ...
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bioRxiv - Microbiology 2022Quote: HEK293 and Vero E6 cell toxicity following fendiline treatment was tested at the indicated time points using the Cell Titer Glo Viability Assay (Promega, Madison WI) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... or SB431542 (1µM) (ALK5 kinase inhibitor) and relative luciferase activities quantified after 16 hours using the Dual-Glo® Luciferase Assay System (Promega®). Histograms were generated and data analyzed by multiple paired t-tests and 2-way ANOVA tests using GraphPad (PRISM® 9.0.0).
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bioRxiv - Cell Biology 2022Quote: ... or combined to K02288 at 1µM (ALK1 kinase inhibitor and relative luciferase activities quantified after 16 hours using the Dual-Glo® Luciferase Assay System (Promega®). The TGFβ Reporter Assay was performed in TMLCs after transfection of the various PTPN14 constructs ...
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bioRxiv - Cell Biology 2022Quote: ... The viability of hUC-MSCs after 24 h exposure to increasing concentrations of GNRs was determined by the CellTiter-Glo™ Luminescent Cell Viability Assay (Promega Corporation), which generates luminescent signals based on ATP levels ...
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bioRxiv - Microbiology 2020Quote: ... Transfection efficiency was normalized through co-transfection of a lacZ reporter construct and determination of β-galactosidase activity using a commercial β-galactosidase enzyme assay system (Promega).
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bioRxiv - Microbiology 2020Quote: ... The cells were then incubated for 24 hours at 37°C in 5% CO2 and the luciferase activity was measured using the ONE-Glo™ luciferase assay (Promega, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The cells were incubated at 37°C in 5% CO2 for 24 hours and luciferase activity was measured using a ONE-Glo™ luciferase assay (Promega, USA) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2020Quote: ... The NanoLuc and Fluc luciferase activities were measured 3 days after transfection using the Nano-Glo® Dual-Luciferase® Reporter Assay System (Promega). The firefly (Fluc ...
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bioRxiv - Cancer Biology 2020Quote: ... The membrane was then carefully cut and the numbers of PBMCs that had migrated towards the bottom chamber were quantified using a Cell Titer Glo assay (Promega, Madison, WI) using a standard curve to calculate cell numbers.
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bioRxiv - Biochemistry 2021Quote: ... Cells were harvested two days post-transfection and lysed using the Passive Lysis Buffer provided in the Dual-Luciferase Reporter Assay System (Promega, Madison, WI). The relative activities of the firefly luciferase and renilla luciferase domains in the clarified lysate were then measured using the Dual-Luciferase Reporter Assay System on a Synergy Neo2 plate reader (Biotek ...
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bioRxiv - Microbiology 2021Quote: Cytotoxicity in Huh7 knock-out cell lines and upon inhibitor treatment of Huh7 and VeroE6 cell lines was monitored using CytoTox 96® Non-Radioactive Cytotoxicity Assay (Promega, G1780). Relative cytotoxicity compared to lysed control cells was analyzed ...
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bioRxiv - Microbiology 2022Quote: ... subjected to a freeze-thaw cycle to lyse cells and luciferase activity was measured using the Luciferase Assay System (Promega, Madison, WI) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Pelleted cells were incubated with ONE-Glo reagent for 30 minutes at room temperature following the ONE-Glo Luciferase Assay System protocol from Promega (Catalog # E6130). Luminescence was quantified by the Synergy H1 Hybrid reader (BioTek ...
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bioRxiv - Cancer Biology 2022Quote: Apoptosis induction in response to SRRM1 silencing in leukemia cell lines (25,000 cells/well onto white-walled multiwell luminometer plates) was performed by using Caspase-Glo® 3/7 Assay (Promega Corporation, #G8091) as previously reported (67) ...
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bioRxiv - Cancer Biology 2019Quote: ... Cell viability was assessed using the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) CellTiter 96 Aqueous One Solution Cell Proliferation Assay (Promega, Madison, WI) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... The cell survival ratio was determined by quantifying the reduction in growth compared with mock-treated cells at 48 hours using a standard MTT colorimetric assay (Promega AQueous One). The assay was repeated four times.
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bioRxiv - Evolutionary Biology 2019Quote: ... and luciferase/Renilla signal was quantified on a GloMax®-Multi+ dual injector luminometer using a Dual-Glo® Luciferase Assay system (Promega). To determine the effect of increasing the amount of Tbx2a co-transfected on luciferase expression we transformed the measure of luciferase/Renilla for each co-transfected reaction relative to the average measure of luciferase/Renilla of the same plasmid that was not co-transfected with Tbx2a ...
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bioRxiv - Bioengineering 2019Quote: ... gels were homogenized in lysis buffer using a hand grinder and were measured using the CellTiter-Glo luminescent Cell Viability Assay (Promega, United States) and the Quant-iT PicoGreen dsDNA assay (ThermoFisher ...
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bioRxiv - Microbiology 2020Quote: ... cells were lysed in 100 μL of passive lysis buffer and 50 μL lysate was incubated with 100 μL of luciferase assay substrate according to the manufacturer’s instructions (Promega, Madison, WI, USA).
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bioRxiv - Cancer Biology 2020Quote: Apoptotic events after treatment with liposomal siRNA and/or chemotherapy regimens were determined by the TdT-mediated dUTP nick end labeling (TUNEL) assay (Promega, Madison, WI) in tumor sections ...
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bioRxiv - Cancer Biology 2020Quote: ... The selected cells were tested for AR signaling pathway activation in response to DHT treatment after drug treatment using Brightglo luciferase assay from Promega (Cat no.E264A). The cells were collected into Eppendorf tube and divided into two equal aliquots ...
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Fatty acid binding proteins shape the cellular response to activation of the glucocorticoid receptorbioRxiv - Pharmacology and Toxicology 2021Quote: ... Media samples were taken to detect the secreted alkaline phosphatase reporter (GRE-SEAP) before the cells were lysed and assayed for β-galactosidase activity using the β-galactosidase enzyme assay system (Promega) to correct for differences in transfection efficiency ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: To determine Caspase 3/7 activity in THP-1 SAMHD1 KO and CTRL cells the Caspase-Glo 3/7 assay (Promega, Walldorf, Germany) was used according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... virus replication was quantified by measuring Renilla luciferase activity (relative light units [RLU]) using Bright-Glo™ Luciferase Assay System (Promega, USA) 48 hours post-reinfection ...
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bioRxiv - Microbiology 2019Quote: ... then the GFP expression level was observed under a fluorescent microscope and luciferase activity was measured using a Renilla Luciferase Assay System (Promega, Fitchburg, WI). The detailed methodology is described in the luciferase assay section.
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bioRxiv - Microbiology 2019Quote: ... Virus infectivity was quantified by measuring Renilla luciferase activity (relative light units [RLU]) using Bright-Glo™ Luciferase Assay System (Promega, USA) on the Tecan microplate reader (Tecan Infinite® 200 Pro ...
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bioRxiv - Immunology 2021Quote: The effect of neutralization capacity of Multivalent DARPin was evaluated by exposing serial dilutions of the DARPin candidates to increasing titers of SARS-CoV-2 and determining cell protection by CellTiter-Glo assay (Promega, Madison, USA). Serial dilution of DARPin candidates were prepared in 96 well plates in 100 µl cell culture medium (2%-FBS-MEM + HSA ...
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bioRxiv - Immunology 2020Quote: Caspase-1 and Caspase 3/7 activities were measured using Caspase-Glo® 1 and Caspase-Glo® 3/7 assay (Promega) respectively ...
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bioRxiv - Microbiology 2021Quote: ... the cells were lysed in 100 μL of passive lysis buffer and 50 μL lysate was incubated with 100 μL of luciferase assay substrate according to the manufacturer’s instructions (Promega, Madison, WI, USA).
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bioRxiv - Microbiology 2021Quote: ... Luciferase activity in the infected cells was measured by removing the culture supernatant and adding 50 μL Luciferase assay reagent (Firefly luciferase reagent, Promega. Madison, WI). Luminescence was recorded using the luminescence plate reader (Clariostar plate reader ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 100 µL of the developed reagent was transferred to a solid white 96-well assay plate and fluorescence was read using a multi-modal plate reader (Ex 520nm/Em 580nm; GloMax Explorer, Promega, WI, USA).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and 48h post-transduction the luciferase activity was read by adding luciferase substrate (ONE-Glo EX luciferase assay system, Promega, Wisconsin, USA) using a luminescence plate reader (Envision ...
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bioRxiv - Cancer Biology 2021Quote: The effect of P2RX7B expression and inhibition on OS cell proliferation was assessed using the CellTiter 96® AQueous One Solution Cell Proliferation Assay (Promega, UK), TE85 ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell viability was determined for the serially diluted cells 5 days after seeding with the Cell Titer Glo assay (Promega, cat# G7573) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were collected 24 hours after transfection and mixed with equal volume of Nano-Glo® Luciferase Assay reagent and the relative luminescence (RLU) was measured after 7 minutes using GloMax® 20/20 Luminometer (Promega). The identical cell lysate was then used for protein concentration determination using Bicinchoninic Acid Protein Assay Kit (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: Cellular ROS was measured through the detection glutathione in its reduced (GSH) and oxidized (GSSG) forms using the luminescence-based GSH/GSSG-Glo™ Assay (Promega, V6611). Briefly ...
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bioRxiv - Molecular Biology 2021Quote: ... a standard 12.5 μl translation reaction was mixed with 50 μl 1x Renilla-Glo substrate in Renilla-Glo assay buffer (Promega, Cat. No. E2720) on a white-bottom 96-well plate (Greiner ...
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bioRxiv - Microbiology 2020Quote: ... medium was removed from the cells and replaced by MTS reagent (CellTiter 96 AQueous One Solution Cell Proliferation Assay from Promega, Madison, WI) diluted in PBS ...
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bioRxiv - Microbiology 2020Quote: ... Pseudovirus titers were measured by infecting different cells for 48 h prior to measuring luciferase activity (luciferase assay reagent, Promega, Madison, WI), as described previously (51) ...
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bioRxiv - Immunology 2020Quote: ... aliquots of the remaining cell suspension were used to determine cell counts for each sample using a CellTiterGlo luminescence assay (Promega, Madison, WI). Briefly ...
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bioRxiv - Developmental Biology 2021Quote: ... in media containing 1% FBS and tested for expression of luciferase using the ONE-Glo™ + Tox Luciferase Reporter and Cell Viability Assay (Promega #E7120). Luciferase expression was measured using a Flexstation 3 plate reader (Molecular Devices) ...
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bioRxiv - Microbiology 2020Quote: The effect of investigated compounds on the metabolic activity of the cells was analyzed using the CellTiter-Glo® Luminescent Cell Viability Assay (Promega #G7571). Metabolic activity was examined under conditions corresponding to the respective infection assays ...