Labshake search
Citations for Promega :
6451 - 6500 of 9065 citations for Total Carbohydrate Microplate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: Cytotoxicity of macrophages after 8 hours of infection was quantified by measuring the release of the enzyme lactate dehydrogenase in cell supernatants using the CytoTox 96 Non-Radioactive Cytotoxicity Assay (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... cells were washed in 1 x PBS and cell viability was assessed using CellTiter-Glo Luminescent Cell Viability Assay (Promega). Luminescence read-out was normalized to signal from vehicle treated cells and represented as percentage of viable cells compared to vehicle ...
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bioRxiv - Immunology 2021Quote: Cell death was assessed by measuring lactate dehydrogenase (LDH) release into the supernatant using a CytoTox 96 Non-Radioactive Cytotoxicity Assay (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... 100 mL of media was removed and replaced with 100 mL of Bright-Glo Luciferase Assay Reagent (Promega, Cat # E2620). Luciferase activity was measured on the EnSpire Plate Reader (PerkinElmer) ...
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bioRxiv - Microbiology 2021Quote: ... Cells were lysed and luciferase activity was assessed using the Luciferase Assay System and the Glomax 20/20 luminometer (Promega). Infectivity values were analyzed and plotted using Prism 8.
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bioRxiv - Molecular Biology 2021Quote: Cells were harvested two days after transfection and firefly and Renilla luciferase activities were measured using the Dual-Luciferase reporter assay system (Promega). Total RNA was isolated using TriFast (Peqlab biotechnologies) ...
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bioRxiv - Molecular Biology 2021Quote: ... Luciferase assays require washing the harvested cells 3 times in PBS prior to lysis in passive lysis buffer (Promega, E1941). NLuc activity was assayed using the Nano Luciferase Assay Kit (Promega ...
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bioRxiv - Microbiology 2021Quote: ... The reading of luminescence was performed using the Nano-Glo® Luciferase Assay System according to the manufacturer’s instructions (Promega). After 3 minutes of incubation ...
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bioRxiv - Immunology 2020Quote: ... Recombinant DJ-1 protein (1 μM) was added to the transfected HEK293 cell lines and luciferase activities were measured using a Luciferase Assay System (Promega). 10 μg mL−1 of peptidoglycan (Sigma-Aldrich) ...
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bioRxiv - Plant Biology 2020Quote: ... The luciferase assay was performed by thoroughly mixing 10 μl of a sample with 50 μl of the luciferin substrate (Luciferase Assay System, Promega) and measuring the luminescence intensity after 10 and 20 seconds using a luminometer (TD-20/20 Luminometer ...
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bioRxiv - Immunology 2021Quote: ... extensively washed to remove unbound virus and co-cultured with Huh7.5 for 5 days at 37°C after which they were analyzed for with the Luciferase assay system (Promega, USA) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and luciferase activity was quantified in a Tristar LB 941 luminometer (Berthold Technologies, Bad Wildbad, Germany) using Renilla Luciferase Assay System (Promega) as recommended by the manufacturer.
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bioRxiv - Cell Biology 2021Quote: ... Aliquots of culture medium (300 μl) were taken at indicated timepoints and mixed with 100 μL of 4X RealTime-Glo extracellular ATP assay reagent (Promega) reconstituted in RPMI 1640/B27(-Ins ...
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bioRxiv - Immunology 2021Quote: ... caspase-1 activity in the cell culture supernatants was measured using Caspase-Glo® 1 inflammasome assay according to the manufacturer’s instructions (Promega). Luminescence was measured using an Omega plate reader.
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bioRxiv - Cancer Biology 2021Quote: ... cells were lysed in the plate with 250 μl of 1x Passive Lysis Buffer and luciferase activity was determined using the Dual-Luciferase Reporter Assay System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: The viability of cells was measured after six days exposure to various concentrations of drugs using the Cell Titre-Glo assay (Promega). Long-term drug exposure effects were assessed by colony formation assay after 12–14 days exposure to a drug-containing medium (refreshed weekly ...
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bioRxiv - Microbiology 2022Quote: ... Germany) for 2.5 s by adding 20 μL of Nano-Glo® substrate in assay buffer (Promega Inc, WI, USA). Uninfected monolayer of Vero cells treated identically served as controls to determine basal luciferase activity for obtaining normalized relative light units ...
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bioRxiv - Microbiology 2022Quote: ... Replicon activity was calculated as the ratio of Renilla (subgenomic reporter) to Firefly (co-transfected loading control RNA, cap-dependent translation) using Dual Luciferase Stop & Glo Reporter Assay System (Promega) and normalized by the same ratio for the control wt replicon ...
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bioRxiv - Genomics 2022Quote: ... Caspase 3/7 activity was measured 16 and 24 h later using the Caspase-Glo 3/7 Assay System (Promega) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: Methylation of peptides (PSL, Heidelberg, Germany, Table S5) was performed using the bioluminescence-based MTase-GloTM Assay (Promega, Cat#V7601) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... Luciferase expression was measured at 8 hr (for translation) and 72 hr (for replication) using the Renilla Luciferase Assay System (Promega). Renilla luciferase luminescence was measured using a Clariostar Microplate Reader (BMG Labtech ...
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bioRxiv - Biochemistry 2022Quote: ... The 90% buffer was removed and protoplasts were flash-frozen in liquid nitrogen to quench the protein degradation process followed by luciferase activity assay (E1500, Promega) to determine the relative amount of RAP2.12(1-28)-luciferase chimera protein remaining at various time points ...
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bioRxiv - Cancer Biology 2022Quote: ... Rescue assays were performed in media supplemented with 10%(v/v) FBS as previously described using Cell Titer 96 MTS (Promega). Briefly ...
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bioRxiv - Cancer Biology 2022Quote: ... cell lysates were prepared to consecutively measure the firefly and Renilla luciferase activities using Dual-luciferase reporter assay system (Promega) on INFINITE 200 Pro multimode reader (TECAN) ...
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bioRxiv - Cancer Biology 2022Quote: Cell viabilities and Caspase 3/7 activities were measured via Cell Titer-Glo (CTG) Luminescent Cell Viability Assay (Promega, USA) or Caspase-Glo® 3/7 (Promega ...
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bioRxiv - Immunology 2022Quote: ... 100 μl of ATP-depleted faecal suspension was mixed with 100 μl BacTiter-Glo™ Microbial Cell Viability Assay (Promega) in a clear ...
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bioRxiv - Genomics 2022Quote: ... Firefly (transfection control) and Renilla (target) luciferase activities were sequentially measured using the Dual-Glo Luciferase Assay System (Promega E2920) and detected on a Spectramax M plate reader (Molecular Devices) ...
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bioRxiv - Immunology 2022Quote: ... and a freeze-thay cycle and Firefly Luciferase activity was assessed using the Firefly Luciferase Assay following the manufacturer’s recommendations (E4550 from Promega, Belgium). Percent infection was calculated relative to infected cells in the absence of test serum ...
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bioRxiv - Immunology 2022Quote: ... cells were treated with experimental media containing metabolites and drugs as described in figure legends for 16 hours and then luciferase signal assessed using the Steady-Glo luciferase assay system (Promega) detected using a luminometer ...
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bioRxiv - Immunology 2022Quote: ... or HT1080/Ace2 cl14 cells (for all variant neutralization assays) for 48 hours after which cells were washed with PBS and lysedwith Luciferase Cell Culture Lysis 5× reagent (Promega). Nanoluc Luciferase activity in lysateswas measured using the Nano-Glo Luciferase Assay System (Promega ...
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bioRxiv - Neuroscience 2022Quote: ... supernatant was collected and protein concentration quantified using the 660 nm Protein Assay Reagent (Pierce) supplemented with 50 mM Ionic Detergent Compatible Reagent (Pierce) in a GloMax Multi Detection Plate Reader (Promega). After boiling in 1X Laemmli Sample Buffer (Bio-Rad ...
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bioRxiv - Immunology 2022Quote: ... Cell viability was assessed using a Cell Titer 96 AQueous One Solution Cell Proliferation Assay (Promega Corp, Madison, Wisconsin, USA). Absorbance measurements at 490 nm were normalized to the maximum read per cell line ...
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bioRxiv - Immunology 2022Quote: ... Plates were scored for CPE five days post infection using the CellTiter-Glo® Luminescent Cell Viability Assay per the manufacture’s protocol (Promega). TCID50 was calculated using the Reed and Muench method (67).
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bioRxiv - Synthetic Biology 2022Quote: ... Expression of Firefly luciferase (FLuc) and Renilla luciferase (RLuc) were determined with the Dual-Glo® Luciferase Assay System (Promega) following manufacturer’s instruction with some modifications ...
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bioRxiv - Microbiology 2022Quote: ... The cells were incubated for 24 hrs at 37 °C (for HEK-293T) or 39 °C (DF-1) and luciferase activity was measured by using the Dual-Glo luciferase assay system (Promega). The polymerase activity was calculated by normalising firefly luciferase activity relative to the Renilla luciferase activity.
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bioRxiv - Microbiology 2022Quote: Cell viability of different 2D cultures at different time points of infection or exposure to virus proteins or HAE supernatant was determined using the CellTiter 96 AQueous One Solution cell proliferation assay (G3582; Promega), while HTO viability was determined by the CellTiter-Glo 3D cell viability assay (Promega G9681 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Kinase activity of phosphorylated CDK2 with cyclin A and varying amounts of inhibitor (dinaciclib or 5) were measured using the ADP-Glo Kinase Assay (Promega) in a luminescence plate reader (Tecan Infinite M1000 PRO) ...
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bioRxiv - Microbiology 2022Quote: Purified recombinant His6-SBP-tagged HipBLp, HipSLp, and HipTLp (WT, D197Q, D219Q) were tested for ATP hydrolytic activity using the ADP-Glo kinase assay (Promega) per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... used as PCR template and directly for luminescence quantification according to the manufacturer’s instructions (20 μL phage plus 20 μL substrate, Nano-Glo® Luciferase Assay System, Promega).
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bioRxiv - Molecular Biology 2023Quote: ... and firefly luciferase (FLuc) activities were measured by using the Nano-Glo Dual Luciferase Assay on a Glomax Multidetection System (Promega). GFP fluorescence was measured using an Infinite M1000 microplate reader (Tecan Group Ltd) ...
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bioRxiv - Plant Biology 2023Quote: ... Luciferase activity was measured combining by combining 20 μl of sample with 50 μl of enzyme substrate (Luciferase 1000 Assay System, #E4550, Promega), vortexing sample briefly and counting photons for 5 s in the luminometer (Luminoskan TL plus ...
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bioRxiv - Microbiology 2022Quote: ... We estimated the number of infectious virus after two days by quantifying the luminescence in each well using Bright Glo Luciferase Assay System (Promega). Differences in relative luciferase units (RLUs ...
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bioRxiv - Microbiology 2024Quote: ... The presence of released LDH in the supernatant was assessed using the Cytotox 96 Non-Radioactive Cytotoxicity Assay (Promega, G1780). The extended protocol can be found in the supplementary materials.
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bioRxiv - Cancer Biology 2024Quote: ... The activity of caspase 3/7 was measured after 48 h using the Caspase-Glo® 3/7 assay (Promega) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... FLuc and RLuc activities in lysates were sequentially measured by using Dual-Glo luciferase assay reagents and a GloMax 20/20 luminometer (Promega) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: ... and dual-luciferase assay performed using homemade reagents (as described in Baker et al., 2014) on the GloMax Discover GM3000 (Promega).
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bioRxiv - Microbiology 2022Quote: ... cells were harvested 24 hours post transfection using 1X passive lysis buffer and the dual luciferase assay was performed according to instructions from manufacturer (Promega). Luciferase signals were read using a BioTek Synergy H1 plate reader (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2022Quote: ... Cells were harvested 24 h of post-transfection and RLuc and FLuc activities were measured by Dual Glo luciferase assay (Promega), according to the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2022Quote: The proliferation of Ba/F3 expressing WT or mutants TpoR mutants was assessed by Cell-Titer-Glo luminescent cell viability assay (Promega). Prior to the experiment ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were lysed and the activities of both Renilla and Firefly luciferases measured on a plate reader using the Dual Luciferase Assay System (Promega). For each cell line quantifications were done in technical triplicates ...