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Citations for Promega :
5951 - 6000 of 8279 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... the cells were lysed in 100 μL of passive lysis buffer and 50 μL lysate was incubated with 100 μL of luciferase assay substrate according to the manufacturer’s instructions (Promega, Madison, WI, USA).
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bioRxiv - Microbiology 2021Quote: ... Luciferase activity in the infected cells was measured by removing the culture supernatant and adding 50 μL Luciferase assay reagent (Firefly luciferase reagent, Promega. Madison, WI). Luminescence was recorded using the luminescence plate reader (Clariostar plate reader ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 100 µL of the developed reagent was transferred to a solid white 96-well assay plate and fluorescence was read using a multi-modal plate reader (Ex 520nm/Em 580nm; GloMax Explorer, Promega, WI, USA).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and 48h post-transduction the luciferase activity was read by adding luciferase substrate (ONE-Glo EX luciferase assay system, Promega, Wisconsin, USA) using a luminescence plate reader (Envision ...
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bioRxiv - Cancer Biology 2021Quote: The effect of P2RX7B expression and inhibition on OS cell proliferation was assessed using the CellTiter 96® AQueous One Solution Cell Proliferation Assay (Promega, UK), TE85 ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell viability was determined for the serially diluted cells 5 days after seeding with the Cell Titer Glo assay (Promega, cat# G7573) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were collected 24 hours after transfection and mixed with equal volume of Nano-Glo® Luciferase Assay reagent and the relative luminescence (RLU) was measured after 7 minutes using GloMax® 20/20 Luminometer (Promega). The identical cell lysate was then used for protein concentration determination using Bicinchoninic Acid Protein Assay Kit (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: Cellular ROS was measured through the detection glutathione in its reduced (GSH) and oxidized (GSSG) forms using the luminescence-based GSH/GSSG-Glo™ Assay (Promega, V6611). Briefly ...
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bioRxiv - Molecular Biology 2021Quote: ... a standard 12.5 μl translation reaction was mixed with 50 μl 1x Renilla-Glo substrate in Renilla-Glo assay buffer (Promega, Cat. No. E2720) on a white-bottom 96-well plate (Greiner ...
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bioRxiv - Microbiology 2020Quote: ... medium was removed from the cells and replaced by MTS reagent (CellTiter 96 AQueous One Solution Cell Proliferation Assay from Promega, Madison, WI) diluted in PBS ...
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bioRxiv - Microbiology 2020Quote: ... Pseudovirus titers were measured by infecting different cells for 48 h prior to measuring luciferase activity (luciferase assay reagent, Promega, Madison, WI), as described previously (51) ...
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bioRxiv - Immunology 2020Quote: ... aliquots of the remaining cell suspension were used to determine cell counts for each sample using a CellTiterGlo luminescence assay (Promega, Madison, WI). Briefly ...
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bioRxiv - Developmental Biology 2021Quote: ... in media containing 1% FBS and tested for expression of luciferase using the ONE-Glo™ + Tox Luciferase Reporter and Cell Viability Assay (Promega #E7120). Luciferase expression was measured using a Flexstation 3 plate reader (Molecular Devices) ...
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bioRxiv - Microbiology 2020Quote: The effect of investigated compounds on the metabolic activity of the cells was analyzed using the CellTiter-Glo® Luminescent Cell Viability Assay (Promega #G7571). Metabolic activity was examined under conditions corresponding to the respective infection assays ...
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bioRxiv - Cancer Biology 2021Quote: ... and viability of the spheroids was determined after 7 days of treatment with the CellTiter-Glo® 3D Cell Viability Assay (Promega G9682) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... the cells were collected and assayed for Firefly and Renilla Luciferase activities using the Dual-Luciferase Reporter Assay System (Promega, WI, USA) and measured in a Genius luminometer (TECAN ...
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bioRxiv - Cancer Biology 2022Quote: ... 1000 and 5000 µM) or remained untreated for 6 days and viability was analyzed using the CellTiter-Glo Luminescent Cell Viability Assay (Promega, Dübendorf, Switzerland) to determine the adenosine triphosphate (ATP ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were treated and then subsequently assessed for their cell viability by the CellTiter-Glo Luminescent Cell Viability Assay (Promega, Cat #G7570). The assay measures ATP as a biomarker of metabolically active cells by the luminescence released from the conversion of Beetle Luciferin + ATP with the enzyme catalyst Ultra-Glo recombinant luciferase and Mg2+ to oxyluciferin + AMP + PPi + CO2 and light ...
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bioRxiv - Cancer Biology 2022Quote: ... Impact of treatments on intracellular ATP content was measured using the CellTiter-Glo® 3D Cell Viability Assay (Promega, cat. no. G9681) at 48 hours (Erastin/YM155 ...
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bioRxiv - Immunology 2022Quote: ... Pseudovirus-containing supernatants were collected 48 hours post transfection and the viral titers were measured by luciferase activity in relative light units (RLU) (Bright-Glo Luciferase Assay System, Promega cat.# E2620). The supernatants were aliquoted and stored at −80°C until further use.
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bioRxiv - Microbiology 2022Quote: ... Following treatment with overnight treatment with LT cells were resuspended in 80 μl of EMEM media and mixed with an equal volume of ONEGLO EX Reagent (Nano-Glo® Dual-Luciferase® Reporter Assay System, Promega) for 20 minutes on an orbital shaker at 300 rpm ...
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bioRxiv - Microbiology 2022Quote: ... the media was replaced with complete MEM (without NH4Cl) and cells incubated an additional 48 hours Transduced (LacZ+) cells were quantified using the Beta-Glo Assay System (Promega, Madison, Wisconsin) following the manufacturers’ protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... SEA (0.2 mg/ml) and soluble schistosomula antigens (0.2 mg/ml) was determined using the Caspase-Glo® 3/7 Assay System (Promega, Sydney, Australia) according to the manufacturers’ instructions.
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bioRxiv - Microbiology 2022Quote: ... the Fluc and NLuc reporter gene expression was measured using a Nano-Glo® Dual-Luciferase® Reporter Assay System (Promega Corporation) following the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... The transfected cells were treated with anthracyclines or Sendai virus (40 hemagglutinin [HA] units/ml) for 24 hours before luciferase activity was determined with the Steady-Glo luciferase assay system (Promega, cat. E2520).
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bioRxiv - Molecular Biology 2022Quote: ... Cells were treated with IFNα (1000 U/mL) for 12 h and were harvested to conduct dual-luciferase reporter assay (Promega, Madison, WI). Firefly luciferase activity values were normalized to Renilla luciferase activity ...
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bioRxiv - Molecular Biology 2022Quote: ... Pelleted cells were disrupted by glass beads agitation at 4°C in 1x Passive Lysis Buffer provided in the Dual-Luciferase® Reporter Assay System (Promega, #E1910). Extracts were clarified by centrifugation ...
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bioRxiv - Neuroscience 2024Quote: ... the transfected cells on a 96-well plate were measured for the luciferase activities using Dual-Glo® Luciferase Assay System (Promega, E2920), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... culture supernatants were frozen at −s80°C and macrophages were processed according to the Promega E1500 Luciferase Assay System protocol (Promega, Madison, WI). Cells were lysed and incubated with D-Luciferin substrate for 5 min in white Lumitrac™ plates (Greiner Bio-one ...
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bioRxiv - Immunology 2024Quote: ... AP20187 dimerizer was added in cell culture media to a final concentration of 500 nM for 5 hours before taking the supernatants for LDH assay (G1780, Promega, Madison, WI). Cell death was measured on a scale with the media without cells set to 0% and cells lysed for 1 hour in 1% Triton X-100 set to 100%.
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bioRxiv - Microbiology 2024Quote: ... and incubated at room temperature with reagent for determination of ATP using BacTiter-Glo Microbial Cell Viability Assay (cat. no. G8232; Promega, Madison, WI), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... measurements 1×105 T cells were cultured in white 96 well plate and analysed using NAD/NADH-Glo™ luminescent assay (Promega, G9071), according to the manufacturer instructions ...
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Mucociliary Clearance Augmenting Drugs Block SARS-Cov-2 Replication in Human Airway Epithelial CellsbioRxiv - Microbiology 2023Quote: ... The plates were incubated at 37°C under 5% CO2 for 144 hour and virus cytopathic effect was measured using the CellTiter-Glo® 2.0 Cell Viability Assay (Promega, Cat. # G9241) following the manufacturer instructions ...
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bioRxiv - Microbiology 2022Quote: ... Luminescence readings were taken at 0 and 72 h post-invasion using the Renilla-Glo® Luciferase Assay System (Promega cat. E2750) and the GloMax® Discover Multimode Microplate Reader (Promega) ...
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bioRxiv - Microbiology 2022Quote: ... the amount of pseudoviruses prepared was quantified by the HiBiT assay using a Nano Glo HiBiT lytic detection system (Promega, Cat# N3040) as previously described32,57 ...
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bioRxiv - Cancer Biology 2023Quote: T cell cytotoxicity induced by BiTEs and BiTE-sialidase fusion proteins was measured by lactate dehydrogenase (LDH) release using CytoTox 96® Non-Radioactive Cytotoxicity Assay (Promega, G1780). 10000 tumor cells and 50000 hPBMCs per well in 100 μL media were exposed to different treatments and incubated in 96 well plates at 37 °C (unless different ratio was specified elsewhere) ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were plated into 96 well plates (3000 cells per well) and cell viability was assessed with a luminescence-based assay (CellTiter Glo, Promega, Cat # G7572) at 48 hours on a SpectraMax M3 plate reader (Molecular Devices) ...
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bioRxiv - Microbiology 2022Quote: Cell viability was measured in uninfected cells by ATP quantification using the Cell Titer Glo luminescent cell viability assay (Promega, Madison, WI). Proliferation was measured after 24 h of treatment of uninfected cells plated at low density (<50% of confluence ...
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bioRxiv - Microbiology 2022Quote: ... the amount of pseudoviruses prepared was quantified by the HiBiT assay using a Nano Glo HiBiT lytic detection system (Promega, Cat# N3040) as previously described35 ...
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bioRxiv - Microbiology 2023Quote: ... cells were cultured together with the compound for 40 hours before luciferase activity in the infected cells was measured using Nano-Glo Luciferase Assay System (Promega, cat. N1120). Inhibition of SARS-CoV-2 variants RIM-1 ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were harvested 24 hours after transfection and luciferases activity was measured using Nano-Glo® Dual-Luciferase® Reporter Assay System (Promega) on Berthold Multimode reader Tristar2S ...
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bioRxiv - Cancer Biology 2023Quote: ... cell viability was determined over time course with a a microplate reader using CellTiter-Glo® 3D Cell Viability Assay (Promega, G9683) according to manufacturer’s directions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The activity of the two luciferase enzymes was measured 24 h after co-transfection following the manufacturer’s instruction of Dual-Luciferase® Reporter Assay System (Cat# E1910, Promega, Wisconsin, USA). Nine biological replications were carried out for each transfection.
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bioRxiv - Cancer Biology 2022Quote: ... cell viability and caspase 3/7 activity were evaluated using CellTiter-Glo and Caspases-Glo 3/7 assays (both from Promega, Madison, WI), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Caspase 3/7 activity was quantified in cell culture by using the Caspase-Glo 3/7 assay system (Promega, Madison, WI, USA). Briefly ...
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bioRxiv - Developmental Biology 2023Quote: ... LacZ reporter activity was assayed in defined media (SD) supplemented with -Leu-Trp using the Beta-Glow® Assay System following commercially available protocols (Promega #E4720). In short ...
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bioRxiv - Bioengineering 2022Quote: CYP3A4 activity of hLiMTs in monoculture or in co-culture with MSCs was assessed using the P450-Glo CYP3A4 Assay (Promega, Dübendorf, Switzerland) with luciferin isopropyl acetal (luciferin-IPA ...
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bioRxiv - Microbiology 2023Quote: ... and CytoTox 96® Reagent was added for 30 minutes according to manufacturers’ instructions (CytoTox 96® Non-Radioactive Cytotoxicity Assay, Promega). After adding Stop Solution ...
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bioRxiv - Microbiology 2023Quote: ... The amount of produced pseudovirus particles was quantified by the HiBiT assay using Nano Glo HiBiT lytic detection system (Promega, Cat# N3040) as previously described14 ...
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bioRxiv - Immunology 2023Quote: ... Cell samples were collected at the indicated times and lysed or assayed using the Dual-Luciferase® Reporter Gene (DLR™) Assay System (Promega) according to the manufacturer’s instructions ...