Labshake search
Citations for Promega :
5751 - 5800 of 8279 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2021Quote: ... Germany) for 2.5 seconds by adding 20 µl of Nano-Glo® substrate in nanoluc assay buffer (Promega Inc, WI, USA). Uninfected monolayer of Vero cells treated identically served as controls to determine basal luciferase activity to obtain normalized relative light units ...
-
bioRxiv - Molecular Biology 2021Quote: Cells were plated on a white 96-well plate (Grenier, 655083) and incubated with RealTime-Glo MT Cell Viability Assay reagent (Promega, G9712). The luminescence was detected by GloMax Navigator.
-
bioRxiv - Microbiology 2021Quote: ... 100 μL of virus-serum mixture was then added to the cells and ~52 hours later luciferase activity was measured using the Bright-Glo Luciferase Assay System (Promega, E2610). Each batch of neutralization assays included a negative control sample of human serum collected in 2017-2018 and a known neutralizing antibody to ensure consistency between batches ...
-
bioRxiv - Microbiology 2021Quote: ... rSARS-CoV-2/Δ7a-Nluc or rSARS-CoV-2/Nluc-2A infected cells was quantified using Nano-Glo® Luciferase Assay System (Promega) following the manufacturers’ specification.
-
bioRxiv - Molecular Biology 2021Quote: ... 20 μL of supernatant was transferred to a new plate and assayed for luciferase activity using the Nano-Glo Dual-Luciferase Reporter Assay System (Promega, N1610) to measure Firefly (Fluc ...
-
bioRxiv - Molecular Biology 2021Quote: Media from transiently transfected HEK293T cells was aspirated and cells were lysed in 40 μL of 1x passive lysis buffer from the Dual-Luciferase Reporter Assay System (Promega, E1980) and either directly assayed or frozen at −20°C ...
-
bioRxiv - Immunology 2020Quote: Viable cell numbers were quantified either by Trypan blue exclusion or by the production of formazan product (OD490) 2 hours after addition of CellTiter 96® Aqueous One Solution assay reagent (Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2020Quote: ... cells were lysed and luciferase activity (RLU, relative light units) was measured using the Bright-Glo™ Luciferase Assay System (Promega). Background luminescence produced by cells-only controls (no pseudotyped virus ...
-
bioRxiv - Biochemistry 2021Quote: ... After treatment with the specified compounds cells were harvested and luciferase emission measured using Dual-Glo® Luciferase Assay System (Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2021Quote: ... Cell viability following 72 h treatment of cells with the respective compounds was determined via CellTiter-Glo® Luminescent Cell Viability Assay (Promega), following manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2021Quote: ... inhibition following 42 h treatment of cells with the respective compounds was determined via CellTiter-Glo® Luminescent Cell Viability Assay (Promega). Luminescent signal was recorded using a CLARIOstar multi-mode microplate reader (BMG Labtech ...
-
bioRxiv - Neuroscience 2020Quote: Cell cytotoxicity was detected by measuring lactate dehydrogenase (LDH) levels using the CytoTox 96® Non-Radioactive Cytotoxicity Assay (Promega, G1780). The manufacturer’s instructions were followed and the absorbance reflecting the LDH content in the cell supernatant was measured at 492 nm (600 nm reference ...
-
bioRxiv - Cancer Biology 2020Quote: ... Viability measurements were read after 1 hour (for baseline) and after 24-96 hours with RealTime-Glo™ MT Cell Viability Assay (Promega).
-
bioRxiv - Cancer Biology 2021Quote: ... Plates were then incubated at 37°C in 5% CO2 and cell viability was assessed at the indicated time points by measuring ATP content through Cell Titer-Glo® Luminescent Cell Viability assay (Promega) over 22 and 32 days of constant treatment (for WiDr and DiFi respectively) ...
-
bioRxiv - Cell Biology 2020Quote: ... and Gaussia luciferase was assayed from 1:40 diluted cell culture supernatant using either the Stop & Glo reagent from the DualLuciferase® Reporter Assay System (Promega) or BioLux® Gaussia Luciferase Assay Kit (New England Biolabs ...
-
bioRxiv - Cancer Biology 2021Quote: ... Drug combinations were performed by treating cells with varying concentrations of the drugs/inhibitors (Table S1) and cell viability was measured by CellTiterGlo assay (Promega, G7572).
-
bioRxiv - Microbiology 2020Quote: ... culture media was removed from the cells and cell viability was measured using a CellTiter-Glo® Luminescent Cell Viability Assay per manufacturer’s instructions (Promega, G7572). Luminescence was measured using a Beckman Coulter DTX 880 multimode plate reader.
-
bioRxiv - Immunology 2021Quote: The cytotoxicity of drugs on the cultured cells was measured by the release of LDH (an indicator of cytotoxicity) using a CytoTox 96 Non-Radioactive Cytotoxicity Assay (Promega, G1780) following the provided technical bulletin.
-
bioRxiv - Molecular Biology 2021Quote: ... and another 25 μL of cell lysate was mixed with 25 μL of ONE-Glo Luciferase Assay System (Promega, Cat.# E6130). The plate was placed on a shaker for 5 min in the dark ...
-
bioRxiv - Microbiology 2021Quote: ... target cells were plated at 2.5 × 104 cells per well in 96-well plates or at 2 × 105 cells per well in 12-well plates and infected for 24-48 h before lysis and Renilla (and Firefly) luciferase activity measure (Dual-Luciferase® Reporter Assay System Promega) or fixation with 2% paraformaldehyde (PFA)-PBS ...
-
bioRxiv - Microbiology 2021Quote: The cell viability of Vero E6 and Calu-3 cells was analyzed by quantification of ATP levels using the CellTiter-Glo™ Luminescent Cell Viability assay (Promega) according to the instructions of the manufacturer ...
-
bioRxiv - Microbiology 2023Quote: ... and cellular expression of the luciferase reporter indicating viral infection was determined using the ONE-Glo™ Luciferase Assay System (Promega) 48 h after infection ...
-
bioRxiv - Cell Biology 2022Quote: After extraction of proteins (see above) without proteinase and phosphatase inhibitors the Src activity was evaluated with ProFluor® Src-Family Kinase Assay (Promega).
-
bioRxiv - Microbiology 2023Quote: ... The migrated cells in the lower chamber were stained with 5 μl of CellTiter 96 AQueous One Solution Cell Proliferation Assay (Promega # G3580) for 2.5 h at 37° C with 5% CO2 ...
-
bioRxiv - Molecular Biology 2022Quote: ... Viability of PBMC following exposure to test items was assessed by resazurin reduction assay (CellTiter-Blue Reagent, Promega, Madison, WI, USA). Cytotoxicity was assessed by measurement of lactate dehydrogenase in the cell culture supernatant (CyQUANT™ LDH Cytotoxicity Assay ...
-
bioRxiv - Neuroscience 2023Quote: ... 10µl of luciferase containing culture medium was transferred into a fresh 96 well plate and incubated with either Furimazine (Nano-Glo™ Luciferase Assay, Promega)/Coelenterazine (Prolume ...
-
bioRxiv - Cancer Biology 2023Quote: ... The firefly and renilla luciferase activity assessment were conducted 48h post-transfection using the Dual-Glo® Luciferase Assay System (Promega), according to the manufacturer’s protocol and the GloMax® Discover Microplate Reader (Promega).
-
bioRxiv - Biochemistry 2023Quote: ... nLuc luciferase signal was measured by mixing 25 μL of Nano-Glo Luciferase Assay System (prepared 1:50 as recommended; Promega # N1120) with 25 μL of diluted reactions (diluted 1:5 in Glo Lysis Buffer ...
-
bioRxiv - Biochemistry 2023Quote: ... 25 μL diluted lysate was mixed with an equal volume of Renilla-Glo Luciferase Assay System (prepared 1:100 as recommended; Promega # E2710) for 10 min at room temperature.
-
bioRxiv - Cancer Biology 2023Quote: ... Cell viability of (non-)treated tumor spheroids was evaluated using the CellTiter-Glo 3D Cell Viability Assay (Promega, Fitchburg, WI, USA) according to the manufacturer’s instructions with slight modifications ...
-
bioRxiv - Molecular Biology 2023Quote: ... Produced pseudoviruses were titrated on HEK-293T-ACE2 by performing duplicate serial dilutions and virus titers were measured 48 hours after infection using Bright-Glo Luciferase Assay System (Promega, E2610).
-
bioRxiv - Microbiology 2023Quote: ... The presence of glycosylated proteins in the cell lysates was assessed using Promega Endoglycosidase H (Endo H) assay reagents (Promega V4871). Briefly ...
-
bioRxiv - Microbiology 2023Quote: ... The amount of pseudoviruses prepared was quantified by the HiBiT assay using Nano Glo HiBiT lytic detection system (Promega,Cat# N3040) as previously described.1–5 ...
-
bioRxiv - Evolutionary Biology 2023Quote: Target gene and AAEL002401(reference gene) expression was quantified by RT-qPCR using FAM- and HEX -labelled GoTaq® Probe qPCR and RT-qPCR assay (Promega), TaqMan universal PCR master mix (Promega ...
-
bioRxiv - Microbiology 2023Quote: The cytotoxic activity on Hela cells of each bacterial strain was analyzed using the CellTiter-Blue® Cell Viability Assay (Promega) following manufacturer instructions ...
-
bioRxiv - Developmental Biology 2023Quote: ... and 1:2000 RealTime-Glo MT Cell Viability Assay Substrate (to visualise Nluc, first diluted 1:1 in DMSO, Promega G9711).
-
bioRxiv - Microbiology 2023Quote: ... LgBit and SmBiT complementation generated luminescent signal was measured in live cells using Nano-Glo® Live Cell Assay System (#N2011, Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2023Quote: Cell viability was determined at DIV 10 using the CellTiter-Blue Cell Viability Assay according to the manufacturer’s instructions (ref: 8080, Promega, Madison, WI). In brief ...
-
bioRxiv - Neuroscience 2022Quote: ... The cerebral cortex was dissected and assayed for Luc activity as described (27) using an assay system reagent (Promega, Madison, WI) on a Lu-minoskan Ascent luminometer (Thermo Electron Corp ...
-
bioRxiv - Microbiology 2022Quote: Activity of caspases-3/7 and -8 were assessed using the corresponding Caspase-Glo® Assays in white-walled 96-well plates (Promega).
-
bioRxiv - Microbiology 2022Quote: ... the beads were washed three times and bound NanoLuc activity measured using Nano-Glo Luciferase Assay System and a GloMax® Navigator Microplate Luminometer (Promega).
-
bioRxiv - Bioengineering 2022Quote: Cytochrome CYP3A4 enzymatic activity of HLCs was measured using P450-Glo™ Assay with Luciferin-IPA (catalogue no. V9001, Promega, Sweden). The relative luminescence was normalized to total protein content ...
-
bioRxiv - Cancer Biology 2024Quote: ... Cell viability was quantified by detection of ATP content in the culture medium with CellTiter-Glo® Luminescent Cell Viability Assay (Promega) by following the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2024Quote: The apoptotic effect of MK-1775 was determined by means of caspase 3/7 activity via Apotox-Glo Triplex Assay (Promega, #G6320) according to the manufacturer’s protocol ...
-
Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... Arc-Hibit signal was identified by complementation with LgBiT (equal volumes) and measurement of Nanoluc-mediated luciferase [JDS1] (Nano-Glo Luciferase Assay, Promega, 565nm) on a microplate reader (SpectraMax iD3).Total Arc in the cell lysate is calculated by dividing the luciferase value of Arc cell lysate by the % of Arc lysate sample used from total sample collected ...
-
bioRxiv - Physiology 2024Quote: ... The toxicity of MNP was determined 24 h upon MNP treatment using the LDH-toxicity Assay (Promega Corp, Fitchburgh, WI, US) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2024Quote: The direct antiviral effect of betacyanin fractions was evaluated through the formation of viral CPE using the Viral ToxGloTM Assay (Promega, USA) per the manufacturer’s instructions with slight modifications ...
-
bioRxiv - Immunology 2024Quote: ... Quantification of SARS-CoV-2 RVP infection was determined using the Renilla-Glo® luciferase assay system (Promega Corp., Madison, WI). Microtiter assay plates were centrifuged for 5 min at 2000 rpm to prevent cell loss ...
-
bioRxiv - Microbiology 2024Quote: ... or 4 μg of the pNL4.3-ΔEnv EGFP backbone36 (for viral entry assay in primary CD4+ T cells) into 293T cells using the FuGENE6 transfection reagent (Promega, USA). The cells were cultured at 37°C for 6 hours before the medium was completely replaced with fresh medium ...
-
bioRxiv - Immunology 2024Quote: ... The virus neutralization titers were determined by measuring the NLuc activity using the Nano-Glo Luciferase Assay System (Promega, Madison, WI) following the manufacturer’s conditions ...