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Citations for Promega :
6101 - 6150 of 8279 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Cell extracts were prepared 48 hours after transfection and luciferase activity was measured using the Dual-Luciferase Reporter assay system (Promega, Madison, WI, USA). Renilla luciferase served as an internal control for normalization.
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bioRxiv - Evolutionary Biology 2021Quote: ... Cells were harvested 3 days after transfection and Firefly and Renilla luciferase activities were measured by using a Dual-Luciferase Reporter Assay System (Promega, Cat. No. E1910). The mean values +/- SD from five independent experiments are shown.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The toxicity of each chemicals was tested on each cell line using the CellTiter-Glo Luminescent Cell Viability Assay (Promega, Madison WI, USA).
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bioRxiv - Cell Biology 2021Quote: ... Firefly luciferase and the Renilla control were detected using the Promega Dual-Luciferase Reporter Assay System per the manufacturer’s protocol (Promega, Madison, WI, cat# E1960). Plates were read on the Victor Nivo plate reader (Perkin Elmer ...
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bioRxiv - Microbiology 2020Quote: ... Cell viability in the presence of the drugs (without infection) was determined based on cellular ATP content using the CellTiter-Glo® Luminescent Cell Viability Assay (Promega, cat#: G7572) according to the manufacturer’s instruction.
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bioRxiv - Microbiology 2021Quote: ... Membrane fusion activity was quantitatively assessed by detecting the expression of firefly luciferase and sea pansy luciferase using the Dual-Luciferase Reporter Assay System (Promega, Madison, WI, USA) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2022Quote: ... Cell viability was determined by removing 100 μL supernatant from all wells and adding 100 μL CellTiter-Glo reagent to the cells as described in the manufacturers protocol (CellTiter-Glo® Luminescent Cell Viability Assay, Promega, Madison, USA). After 2 minutes shaking on an orbital shaker ...
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bioRxiv - Developmental Biology 2022Quote: ... Dual-luciferase assays were performed using the Promega Dual-Luciferase Reporter (DLR™) Assay Systems with the Promega GloMax 20/20 Luminometry System (Promega, Madision, WI) (Stepicheva et al. ...
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bioRxiv - Bioengineering 2022Quote: Induction of apoptosis after 24 h of doxorubicin treatment was quantified by analyzing Caspase 3/7 activity using the Caspase-Glo® 3/7 3D assay (Promega, Madison, WI). The assay protocol was followed from the manufacturer’s website ...
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Farnesyltransferase inhibition overcomes the adaptive resistance to osimertinib in EGFR-mutant NSCLCbioRxiv - Cancer Biology 2022Quote: Viability of untreated cells or treated cells for 5 days was assessed using CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) (Promega®, ref:G3580) according to manufacturer’s instructions.
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bioRxiv - Plant Biology 2021Quote: ... The samples after pull-down assay and co-immunoprecipitation were also digested by 1 µg sequence-grade modified trypsin (Promega, Madison, WI, USA) at 37 °C overnight in the same manner ...
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bioRxiv - Microbiology 2021Quote: ... cells were lyzed and activities of luciferase for each group were detected by Dual-Luciferase® Reporter Assay System (Promega, Madison, WI, USA).
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bioRxiv - Immunology 2020Quote: Activity of the inflammatory caspases 1/4/5 was measured using a commercially available Caspase-Glo® 1 Inflammasome Assay (Promega, WI, USA) from HFFs seeded in 96-well plates (2×104 cells per well) ...
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bioRxiv - Microbiology 2022Quote: ... Episomal expression of the nanoluc reporter gene was confirmed in luminescence assays using NanoGlo substrate according to instructions of the supplier (Promega, Leiden, the Netherlands). Subsequently ...
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bioRxiv - Immunology 2022Quote: ... Concentrated viruses were titrated in serial dilutions with the respective target cell lines and the luciferase reporter signal was determined 48 h later using Luciferase Assay System (Promega, catalog no. E1501) on Cytation 3 (BioTek) ...
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bioRxiv - Bioengineering 2021Quote: ... At time points of 1 h and 48 h samples were tested for cytotoxicity via a commercially available LDH-Glo™ Cytotoxicity Assay (#J2380, Promega, Madison, WI). In brief ...
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bioRxiv - Molecular Biology 2021Quote: ... The transfected cells were cultured at 37°C for 36 h and subjected to luciferase activity analysis using the Dual-Glo Luciferase Assay System (Promega, Madison, WI, USA).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The number of migrated cells was determined by measuring ATP in the lysates of transmigrated cells using a luminescence-based assay (CellTiter-Glo; Promega, Madison, WI, USA) and readouts were taken in the FlexStation 3 Multi-Mode Microplate Reader (Molecular de- vices LLC ...
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bioRxiv - Cancer Biology 2020Quote: ... 1000 cells were seeded in 96-well plates with five replicates and the cell growth capacity was measured every day with the CellTiterBlue assay (#G8080, Promega, Madison, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: TUNEL assays were performed on paraffin-embedded sections of the dorsal skin of embryos using the DeadEnd Fluorometric TUNEL System (Promega, cat. no. G3250) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... CDC was measured after incubation for 3 hours at 37 °C 5% CO2 with a luminometer using the CytoTox-Glo Cytotoxicity Assay (Promega; Cat. Nr.: G9291) according to the manufacturer’s instructions.
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bioRxiv - Genetics 2020Quote: ... The corresponding Firefly luciferase (LUC) and Renilla luciferase (REN) values were measured 16 h after transfection using a Dual-Luciferase® Reporter Assay System (Promega, WI, USA).
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bioRxiv - Molecular Biology 2022Quote: ... Luciferase activity was analyzed using the Dual-Luciferase® Reporter Assay System and read with GloMax®-Multi Detection System (Promega, Madison, WI).
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bioRxiv - Neuroscience 2022Quote: ... and the relative luciferase activity was normalized to Renilla luciferase activity measured by a dual-luciferase reporter assay system (Promega, Madison, WI, USA). SRAMP (http://www.cuilab.cn/sramp/ ...
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bioRxiv - Neuroscience 2023Quote: The intracellular [ATP] was determined by lysing brain capillary ECs and quantifying luminescence produced by luciferase-induced conversion of ATP to light using the CellTiter-Glo Assay 3D (Promega, Madison, WI, USA). Approximately ∼500 ECs were added to each reaction ...
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bioRxiv - Plant Biology 2024Quote: ... the LUC and REN values were measured 24 hours later using the Dual-Luciferase® 80 Reporter Assay System (E1910, Promega, WI, USA)34 ...
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bioRxiv - Cancer Biology 2023Quote: Cell growth and survival was measured using a CellTiter 96® Non-Radioactive Cell Proliferation Assay at an absorbance of 570 nm using (Promega, Mannheim, Germany). Each assay was performed in triplicates and repeated at least 3 times ...
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bioRxiv - Neuroscience 2024Quote: ... The effects of PRMT5 inhibitors on fibroblast cell viability was measured using CellTiter Glo luminescent cell viability assay reagent (Promega, catalogue number: G7570). Cells were grown in 60 µl media ...
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bioRxiv - Molecular Biology 2024Quote: The cytotoxicity of venoms was assessed in Madin-Darby canine kidney II (MDCK II) cells using the CellTiter-Glo Luminescent Cell Viability Assay (Promega, cat. no. G7570) as previously reported (Hurka et al. ...
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bioRxiv - Molecular Biology 2024Quote: Metabolic activity of CpdG or DMSO treated and oexORP7 or oexMYC cells was measured using CellTiter 96® AQueous One Solution Cell Proliferation Assay (Promega cat #: G358C), according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... 100 uL of the diluted lysate was added to each well along with an equal volume of Caspase Glo 3/7 assay reagent (Promega, Madison, Wisconsin, USA), according to the manufacturer’s directions ...
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bioRxiv - Synthetic Biology 2022Quote: Cytotoxicity assay was performed on HCT116 human colon cells (ATCC, #CCL-247™) using CellTiter 96® AQueous One Solution Cell Proliferation Assay (Promega, #G3580) which is a colorimetric method based on MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2- (4-sulfophenyl)-2H-tetrazolium ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: The cytotoxicity of MLNP was evaluated by MTS assay using CellTiter 96® AQueous reagents according to the manufacturer’s protocol (Promega, Madison, WI, USA) and as previously described45 ...
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bioRxiv - Molecular Biology 2023Quote: ... or stimulated for 10 minutes or 1 hour by BRET through activation of NanoLuc’s bioluminescence with its substrate furimazine (1:100 dilution of Promega nano-Glo Live Assay). After treatment ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then infected in quadruplicate and lysed 72 h later using the Bio-Glo luciferase assay system (Cat# GT7940, Promega, Madison, WI, USA) to measure luciferase activity ...
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bioRxiv - Immunology 2023Quote: ... Luciferase expression in cells was measured by lysing the cells in Passive Lysis Buffer and incubating the lysis supernatant with the Luciferase Assay System (Promega, Madison, WI, USA). Luciferase activity was read on an Enspire Multimode Plate Reader (Perkin Elmer) ...
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bioRxiv - Synthetic Biology 2023Quote: ... backbone to contain the large or small fragments of the NanoBiT luciferase (Large BiT or Small BiT) used for the split luciferase complementation assay (Promega, Madison, WI, USA)38 ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were lysed and the cell lysates were prepared according to manual of the Dual-Luciferase® Reporter Assay System (Promega, Madison, USA) and measured with the GlowMax navigator microplate luminometer (Promega ...
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bioRxiv - Biochemistry 2023Quote: The cell free protein expression assays were performed by using the TnT® Quick Coupled Transcription/Translation System (Cat#L1171, Promega, Madison, USA), according to the manufacturer’s guideline with minor modification ...
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bioRxiv - Cancer Biology 2022Quote: ... and West African ancestry HCC1500 (ATCC, CAT: CRL-2329) was assessed utilizing CellTiter-Glo 2.0™ Luminescent Cell Viability Assay (Promega™, CAT: G9242). West African ancestry of the HCC1500 cell line was determined using established Ancestry Informative Markers [38] ...
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bioRxiv - Immunology 2023Quote: T-cell and prodrug cytotoxicity assays with secreted enzymes were performed after 24 to 48 hours using CellTiter-Glo (Promega, Madison, Wisconsin, USA) or luminescence using firefly (ffLuc ...
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bioRxiv - Synthetic Biology 2023Quote: ... 10 uL of supernatant was mixed with 45 μL of dH2O and then mixed with 50 uL of the Nano-Glo® Luciferase Assay system (Promega; catalog# N1110), per the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Luciferase reporter activities in cells co-transfected with any of the luciferase reporter were determined using a luciferase reporter gene assay system (Cat#E1601, Promega, Madison, WI, USA) as instructed ...
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bioRxiv - Cancer Biology 2023Quote: ... The β-Galactosidase activity was measured in 30 µl of lysate incubated with 20 µl of reporter lysis buffer and 50 µl of 2X β-gal Enzyme Assay buffer (Promega, Cat# E2000). The absorbance was read in iMark microplate reader (BioRad) ...
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bioRxiv - Microbiology 2024Quote: Supernatants from non-infected and infected cell cultures were harvested from all the incubation time points and used for determination of the percentage of cell survival using LDH-Glo™ Cytotoxicity Assay (Promega – cat #J2381) kit following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: ... An additional 8 µl of cell lysate was transferred to a 384-well LV plate and ATP was measured by adding 8 µl CellTiter-Glo® Luminescent Cell Viability Assay (Promega Cat. #G7571).
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bioRxiv - Cancer Biology 2024Quote: ... 2 hours after bizine treatment cells were exposed to 250μM or 500μM hydrogen peroxide for 2 min after which glutathione (GSH) levels in cells were assayed according to the manufacturer’s instructions for GSH-Glo™ Glutathione Assay (Promega, Madison, WI, USA).
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bioRxiv - Microbiology 2024Quote: ... Luciferase activity in cell lysates was detected by mixing equal volumes of lysate and Steady-Glo Luciferase Assay System reagent (Promega, Madison, WI, USA) and measured on a Synergy H1 microplate reader (Biotek ...
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bioRxiv - Microbiology 2024Quote: Total oxidized and reduced nicotinamide adenine dinucleotide phosphates (NADP+ and NADPH) were quantified using the NADP/NADPH-Glo™ Assay (Promega, G9081, UK) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 µl of each translation reaction was mixed with 5 µl 1x Renilla-Glo substrate in Renilla-Glo assay buffer and supplemented with 40 µl of H2O (Promega, Cat. No. E2720) on a white-bottom 96-well plate (Greiner ...