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Citations for Promega :
5351 - 5400 of 6667 citations for Osteopontin Human OPN ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... and the three randomly selected variants (AMA22_1 IHCA and AMA23 IHC AVI 4 μg/mL and R AVI 8 μg/mL) was performed following the manufacturer’s instructions using the Wizard Promega kit (Promega, Madison, WI, USA). For whole genome sequencing ...
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bioRxiv - Plant Biology 2024Quote: Total RNA was extracted from roots sampled as above using the SV total RNA isolation kit system following the manufacturer’s instructions (Promega, Madison, WI, USA). cDNA was synthesized with Superscript III reverse transcriptase (Invitrogen® ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: All split luciferase biosensors were made by In-Fusion® HD Cloning kit using firefly luciferase from the pGL3-basic vector (Promega, USA) template ...
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bioRxiv - Molecular Biology 2024Quote: ... tissue homogenization was carried out by bead beating and immediately followed by RNA extraction using the Maxwell® RSC simplyRNA Tissue Kit (Promega AS1340) as per manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: RNA was isolated from islets from ∼15-week-old male mice (WT and Kcnk16 L114P (L/P)) using Maxwell® 16 LEV simplyRNA Purification Kits (Catalog no. AS1280, Promega, USA). RNA integrity was analyzed using an Agilent 2100 Bioanalyzer ...
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bioRxiv - Microbiology 2024Quote: ... Complementary DNA (cDNA) synthesis was performed with oligo-dT primers and random hexamers using the First Strand cDNA Synthesis Kit (Promega, Madison WI) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: ... the supernatant was measured for LDH (Lactate dehydrogenase) release using the kit of CytoTox 96® Non-Radioactive Cytotoxicity Assay (Promega, G1780), according to the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: Total RNA was isolated from 1×106 cryopreserved lymph node (LN) mononuclear cells (LNMC) isolated from LN biopsies using the SimplyRNA Tissue kit for the Maxwell RSC instrument (Promega, Madison, WI). RNA was then tested for both SIV gag and a cellular gene ...
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bioRxiv - Molecular Biology 2023Quote: Genomic DNA was extracted from Tetrahymena WT (SB210, with or without BrdU-labeling) and ΔAMT1 cells using Wizard® Genomic DNA Purification Kit (Promega, A1120), sheared to 3-5kb in length with Megaruptor (Diagenode Diagnostics) ...
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bioRxiv - Biophysics 2023Quote: ... Apoptosis and necrosis assays were performed with a RealTime-Glo Annexin V Apoptosis and Necrosis Assay kit (JA1011, Promega, Madison, WI, USA) at the indicated times after changing the culture medium ...
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bioRxiv - Microbiology 2023Quote: ... Samples where a 1 log decrease in RLU compared to the no-serum virus-only control was observed were then selected for cytotoxicity assay using Cell Titre Glo kit (Promega, Southampton, UK) and light microscopy to verify viability of cells prior to undertaking PVNA (data not shown) ...
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bioRxiv - Bioengineering 2023Quote: ... We then performed first-strand cDNA synthesis from 400 ng total RNA in 20 uL reactions using Promega GoScript Reverse Transcription Kit (Promega, catalog # A5000).
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bioRxiv - Biochemistry 2023Quote: ... Enzymatic activities of KARS wild-type and mutants were determined by measuring AMP production and ATP consumption with AMP-Glo Assay Kit (Cat#V5011, Promega, Madison, USA) and ATP Bioluminescent Assay Kit (Cat#FLAA ...
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bioRxiv - Bioengineering 2023Quote: ... The total ATP contents in static and dynamic cultured Hep3B cell spheroids were measured with the CellTiter-Glo® assay kit (Promega, G9681). Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were lysed 24 h after transfection and luciferase activity was measured with the Dual-Luciferase Reporter Assay kit (Promega Cat. # E1960) according to the manufacturer’s protocol using a FlUOstar plate reader (BMG Labtech) ...
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bioRxiv - Plant Biology 2023Quote: The ATP quantification was performed using ENLITEN® ATP Assay System Bioluminiscence Detection Kit from Promega (protocol by (Yang et al., 2015)) ...
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bioRxiv - Bioengineering 2023Quote: ... The effect on cell viability of PTNP was assessed using the MTS [(3-(4,5-dimethylthiazol-2-yl)-5-(3- carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium)] assay (CellTiter 96 cell proliferation assay kit; Promega, WI, USA) (92) ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA for PCR was isolated from 50 µl of tissue lysate using the Maxwell RSC tissue DNA Kit (Promega, Madison, WI, USA).
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bioRxiv - Cancer Biology 2023Quote: Total RNA from MB cells treated with RA for 48 h was extracted using the SV Total RNA Isolation System kit (Promega, Madison, USA), following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... Single transformed ampicillin resistant colonies were grown in LB + Ampicillin and their DNA was extracted by the Wizard® Plus SV Minipreps DNA Purification System kit (Promega, A1460). Clones with correct insertions were evaluated by Sanger sequencing (LightRun tube ...
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bioRxiv - Immunology 2023Quote: ... the infectivity of the pseudotyped viruses was determined by measuring the luciferase activities using a PicaGene Luminescence Kit (Fujifilm Wako, Osaka, Japan) with a GloMax Navigator Microplate Luminometer (Promega, WI, USA).
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bioRxiv - Genomics 2023Quote: Complete genomic DNA was extracted from fresh overnight growth on Nutrient agar (Oxoid, Hampshire, England, U.K) using a Wizard genomic DNA purification kit (Promega Corporation, Madison, USA) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: DNA in the HHMX-stimulated supernatants of THP1 dual reporter cells was quantified using the QuantiFluor dsDNA Detection Kit from Promega (catalog # E2670) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: RNA isolation from the cultured THP1 dual reporter cells was performed by using ReliaPrep RNA Cell Miniprep Kit (Promega, USA, catalog # Z6011) according to the instructions of the manufacturer ...
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bioRxiv - Microbiology 2023Quote: We isolated the genomic DNA of the strains used in this work using the Wizard genomic DNA purification kit (Promega, WI, USA) and quantified using the Qubit 4 fluorometer (ThermoFisher Scientific ...
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bioRxiv - Pathology 2024Quote: ... amniotic fluid and eluates from swab samples) using the Viral Total Nucleic Acid Purification Kit for the Maxwell RSC instrument (Promega, Madison WI) with the following modifications ...
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bioRxiv - Microbiology 2024Quote: ... Cells infected with viruses expressing luciferase were harvested 4 days after infection and analyzed for luciferase activity with a luminescence kit (Bright-Glo™ Luciferase Assay System, Promega, E2620) as described in (61).
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bioRxiv - Microbiology 2024Quote: ... The PCR products were purified with the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, USA) and quantified using Quantus™ Fluorometer (Promega, USA). Purified amplicons were pooled in equimolar amounts ...
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bioRxiv - Molecular Biology 2024Quote: ... and the ratio of LUC and REN was determined using the of Dual-Luciferase® Reporter Assay System test kit (E1910, Promega, America). All samples were subjected to more than three repeated measurements.
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bioRxiv - Plant Biology 2024Quote: ... Two hundred ng RNA were used to prepare complementary DNA (cDNA) using M-MLV reverse transcriptase kit (Promega, Charbonnières-les-Bains, France) and oligo(dT ...
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bioRxiv - Cancer Biology 2021Quote: ... and 1 μg of the specific lentiviral expression or silencing constructs using FugeneHD (Promega, E2311) in Opti-MEM (Gibco ...
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bioRxiv - Cell Biology 2020Quote: ... Then samples were diluted 3 times in the buffer and 1 µg trypsin (Gold. Promega) was added overnight at 30°C ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were transfected using 1 μg DNA per well and ViaFect™ Transfection Reagent (Promega) in OptiMEM medium (1:3 DNA to reagent ratio) ...
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bioRxiv - Cell Biology 2020Quote: ... the samples were digested overnight at 37°C with 1:100 (w/w) trypsin (Promega). The next day ...
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bioRxiv - Microbiology 2021Quote: ... The lungs were incubated with 1 mg/mL of D-luciferin (Promega, Madison, WI, USA) for 5 min ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and gene expression levels measured using a 1-step RT-qPCR master mix (Promega, UK) and a 7500 Fast qPCR instrument (Applied Biosystems ...
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bioRxiv - Pathology 2020Quote: 0.4 µL GoScriptTM RT Mix for 1-Step RT-qPCR (Promega, Cat # A6120 and A6121)
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bioRxiv - Microbiology 2019Quote: ... Elution of RNA was done in nuclease-free water and 1 μl of rRNasin (Promega) was added before storage.
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bioRxiv - Cell Biology 2020Quote: ... Cells were lysed 48 hr post-transfection using 1 × Passive Lysis buffer (Promega, Madison, USA). Firefly and Renilla luciferase activities were measured using the Dual Luciferase Reporter assay system (Promega ...
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bioRxiv - Systems Biology 2019Quote: ... 1% Triton® X-100 0.1% Na deoxycholate) supplemented with protease inhibitor cocktail (Promega, G6521). Cells were lysed by mechanically means by passaging of cells through 26-gauge needle 5 times ...
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bioRxiv - Neuroscience 2019Quote: ... The mixture was fourfold diluted with ammonium bicarbonate and digested with trypsin (1:100; Promega) at 37 °C ...
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bioRxiv - Pathology 2020Quote: 0.2 µL GoScriptTM RT Mix for 1-Step RT-qPCR (Promega, Cat # A6120 and A6121)
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bioRxiv - Immunology 2021Quote: Viral loads were quantified using the GoTaq® Probe 1-Step RT-qPCR System (Promega). For quantification of SARS-COV-2 the nCOV_N1 primer/probe mix from the SARS-CoV-2 (2019-nCoV ...
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bioRxiv - Neuroscience 2020Quote: ... followed by secondary antibodies conjugated with HRP or alkaline phosphatase (Promega, Madison, WI, 1:1,000). Proteins were visualized with a LAS-3000 mini (GE Healthcare ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 μg of purified RNA was dissolved with 0.5 μg of oligo (dT) primer (Promega), 0.5 mM dNTPs ...
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bioRxiv - Biophysics 2021Quote: ... Cells were then incubated overnight at 4 °C with anti-p75NTR (Promega; G323A; 1:300) and anti-TRADD (Santa Cruz ...
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bioRxiv - Biochemistry 2021Quote: ... The reaction was started by addition of 1:4000 NanoGlo substrate (Promega, catalogue number N1110). The reaction was incubated for 5 minutes and the emission was measured at wavelengths of 445-470 nm and 520-545 nm using Tecan Spark multimode plate reader with luminescence readout and a settle time of 10 ms and integration time of 500 ms ...
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bioRxiv - Biophysics 2021Quote: ... followed by anti-rabbit alkaline phosphatase-conjugated secondary antibody (Promega, S3731, 1:5,000 in TBST) at room temperature for 1 h ...
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bioRxiv - Microbiology 2021Quote: Viral loads were quantified using the GoTaq® Probe 1-Step RT-qPCR System (Promega). For quantification of SARS-COV-2 the nCOV_N1 primer/probe mix from the SARS-CoV-2 (2019-nCoV ...
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bioRxiv - Cell Biology 2021Quote: ... 1 μg of RNA was used for cDNA synthesis using M-MLV reverse transcriptase (Promega). Primer sequences and amplicon sizes are listed in supplementary table 1 ...