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Citations for Promega :
5501 - 5550 of 6667 citations for Osteopontin Human OPN ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... and DNA-concentration was determined using a QuantiFluor® dsDNA kit (detection limit: 50 pg/mL; sensitivity: 0.01 – 200 ng/µL) and Glomax® –Multi+ system (Promega, Madison, WI) with the blue fluorescence optical kit installed (Ex ...
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bioRxiv - Microbiology 2020Quote: ... Extraction of total RNA from viral transport media was performed using the Maxwell RSC Whole blood RNA/DNA kit (Promega Corporation, Madison, WI) on the Maxwell RSC 16 automated nucleic acid extractor ...
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bioRxiv - Microbiology 2020Quote: Faecal DNA was extracted and purified using a combination of repeated bead-beating (method 5) (Costea et al, 2017) and the Maxwell 16 Tissue LEV Total RNA Purification Kit (Promega, Maddison, WI, USA), with STAR (Stool transport and recovery ...
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bioRxiv - Plant Biology 2020Quote: ... and five early developmental buds (one early developmental sample) per individual using the Maxwell 16 LEV Plant RNA Extraction Kit (Promega Corporation, Madison, WI).
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bioRxiv - Molecular Biology 2020Quote: ... and then cloned to ensure representative amplification of the parental copies using pGEM-T Easy-cloning Vector Kit (Promega Corp., Madison, WI, USA) and transformed into competent Escherichia coli JM109 at 42 °C ...
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bioRxiv - Developmental Biology 2020Quote: ... DNAs were purified using the PureYield Midiprep kit and were linearized before in vitro synthesis of anti-sense RNA probes using T7 or Sp6 polymerase (Promega, #P2077 and #P108G), RNAse inhibitor and dig-labeled rNTPs (Roche ...
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bioRxiv - Cancer Biology 2021Quote: ... was measured after 48 and 72 h using the luminescence-based CellTiter-Glo®2.0 cell viability assay kit (Promega, Madison, WI, USA) according to the manufacturer’s instructions with an FLx100 Luminometer (CLARIOSTAR® ...
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bioRxiv - Cell Biology 2022Quote: ... The protein concentration was measured using Pierce™ BCA Protein Assay Kit and GloMax® Explorer Multimode Microplate Reader (Promega, Madison, WI, USA). Expression of OAT1 ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were detached using AccutaseTM and RNA was extracted using the Promega kit ReliaPrep TM RNA Cell Miniprep System (Promega, US. Catalog# Z6011). RNA was reverse transcribed using Omniscipt TM RT kit (Qiagen ...
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bioRxiv - Bioengineering 2022Quote: ... and drug delivery method (IV and TACE) on CYP3A4 activity was tested with the P450-Glo CYP3A4 assay kit (V9001, Promega, Madison, WI, USA) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: Healthy young leaves from each line were collected and genomic DNA was extracted using a Maxwell RSC Plant DNA Kit (Promega, Madison, WI, USA). The presence or absence of the 5.46-kb fragment containing DAI was confirmed by PCR (primers 1 and 2) ...
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bioRxiv - Cell Biology 2022Quote: ... was used as a template in PCR reactions and amplifications were performed with the GoTaq®G2 Flexi DNA Polymerase kit (Promega, Southampton, UK). Cycling parameters were one cycle of 95°C for 5 minutes for initial denaturation followed by touch-down cycling for the first 14 cycles ...
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bioRxiv - Microbiology 2020Quote: ... genomic DNA was isolated from the corresponding BKE (Bacillus Knockout Erythromycin collection - (Koo et al., 2017)) strains using the Wizard® Genomic DNA Purification Kit (Promega, Madison, WI). Of note ...
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bioRxiv - Cell Biology 2020Quote: ... 72 hours post induction the cells were assayed for CYP1A2 and CYP3A4 activity using the P450-Glo CYP3A4 (Luciferin-PFBE) Cell-based/Biochemical Assay kit (Promega, Cat. no. V8902) and the P450-Glo CYP1A2 Induction/Inhibition Assay kit (Promega ...
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bioRxiv - Genomics 2019Quote: ... amplicons from each replicate were pooled and purified with the Wizard SV Gel and PCR Cleanup System kit (Promega Corporation, Madison, Wisconsin, USA).
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bioRxiv - Cancer Biology 2019Quote: ... Cell lines were authenticated at the Duke University DNA Analysis Facility by analyzing DNA samples from each cell lines for polymorphic short tandem repeat (STR) markers using the GenePrint 10 kit from Promega (Madison, WI, USA). HCT116 cell line was a gift from Dr ...
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bioRxiv - Cancer Biology 2020Quote: ... Lipofectamine 3000 was used to co-transfect HEK-293T cells with wild-type and mutant luciferase reporter vectors as well as P51-ETS1-NC or P51-ETS1.Relative luciferase activity was expressed as the ratio of firefly luciferase activity to sea kidney luciferase activity using a dual luciferase reporting kit (Promega, Madison, Wisconsin, USA) after 48 h.
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bioRxiv - Immunology 2020Quote: ... were verified by Sanger sequencing and used as DNA templates in the T7 TNT in vitro transcription/translation kit (Promega, Madison, WI; #L1170) using [35S]-methionine (PerkinElmer ...
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bioRxiv - Pathology 2020Quote: ... cDNA synthesis from the mRNA was performed using a Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) kit (Promega, Madison, Wisconsin, USA) in a Mastercycler® Gradient (Eppendorf ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Total RNA was isolated from EpCAM+ and EPCAM- cell fractions using a Maxwell® LEV simply RNA Cells/Tissue kit (Promega, WI, USA) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... dahliae strain JR2 was isolated from tomato roots seven days after root dip inoculation and following five days of in vitro growth in soil extract and potato dextrose broth (PDB) using the Maxwell® 16 LEV Plant RNA Kit (Promega, Madison, USA). Real-time PCR was performed as described previously17 to determine the expression of effector genes relative to VdGAPDH with primer pairs as shown in Extended data Table 6.
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bioRxiv - Microbiology 2021Quote: ... A 2 ml aliquot of the overnight enrichment was centrifuged at 6000 RPM for 10 minutes and then DNA was extracted using the Maxwell RSC Cultured Cells DNA Kit (Promega, Madison, WI, USA). Extraction was performed according to manufacturer’s recommendations with the addition of RNase A after lysis ...
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bioRxiv - Microbiology 2021Quote: Apical and basolateral samples were used to quantify LDH release in RV-C15-infected HAE with the CytoTox 96® Non-Radioactive Cytotoxicity Assay kit (#G1780; Promega, Madison, WI) following the manufacturer’s protocol.
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bioRxiv - Microbiology 2021Quote: LDH activity and percentage cell cytotoxicity in rabbit lungs (n=3 per group per time point) were determined using the LDH-Glow cytotoxicity kit as per the manufacturer’s instructions (Promega Corporation, Madison, WI, USA). Luminescence was recorded using a GloMax plate reader ...
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bioRxiv - Pathology 2021Quote: ... we first prepared cDNAs from a total RNA sample isolated from an RSV-infected rice plant using the Reverse Transcription Kit as instructed (Promega, Madison, WI, USA). We then amplify the full-length RSV RNA3(- ...
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bioRxiv - Genomics 2020Quote: ... Another 1 ml portion of each spiked enrichment sample was extracted using the Maxwell RSC Cultured Cells DNA kit with a Maxwell RSC Instrument (Promega Corporation, Madison, WI) according to manufacturer’s instructions for Gram-negative bacteria with additional RNase treatment ...
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bioRxiv - Physiology 2019Quote: ... The wounded area and its surrounding area (2 x 2 cm) were immediately dissected out and RNA extraction was conducted using Maxwell® RSC simplyRNA Tissue Kit (AS1340, Promega, Madison WI) following the manufacturer’s protocol ...
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bioRxiv - Physiology 2019Quote: ... To ensure RNA was free of DNA contamination samples were DNase treated using an RQ1 RNase-free DNase kit (Promega Corporations, Madison, USA).
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bioRxiv - Evolutionary Biology 2021Quote: ... We extracted genomic DNA from 400 µL of these cultures with the Maxwell® RSC Cultured Cells DNA Kits using a Maxwell® RSC Instrument (Promega, Switzerland), at the Functional Genomics Centre Zurich (FGCZ) ...
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bioRxiv - Biochemistry 2021Quote: ... Amplified PCR products were visualized by blue light illumination and the 504/505 bp bands were excised and the amplified PCR product purified using the Wizard SV Gel and PCR Clean-Up kit (Promega, Madison, WI, USA). Extracted DNA was Sanger sequenced at the University of Michigan Advanced Genomics Core and analyzed for presence of the single base pair deletion using DNAStar LaserGene SeqMan Pro 15 ...
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bioRxiv - Biochemistry 2021Quote: The kinase activity of the FLT3-ITD protein was measured by using ADP-Glo™ Kinase Assay Kit (Promega, Madison, Wisconsin, the US). Briefly ...
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bioRxiv - Cancer Biology 2021Quote: Genomic DNA was extracted from FFPE tumor tissue samples of 18 patients within the cohort using the automated Maxwell system with the Maxwell 16 FFPE Plus LEV DNA Purification Kit (Promega, Madison, WI, USA), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: RNA was extracted from FFPE tissue samples using the automated Maxwell system with the Maxwell 16 LEV RNA FFPE Kit (Promega, Madison, WI, USA), according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... All RACE-PCR products were purified using GeneJET Gel Purification Kit (Fermentas, Hanover, MD, USA) and ligated into pGEM-T vector (Promega, Madison, WI, USA) and transformed into competent JM-109 Escherichia coli cells (Promega ...
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bioRxiv - Microbiology 2020Quote: ... Single-cell clones were obtained and the genomic DNA was then isolated using a Wizard SV genomic DNA purification kit (Promega, Madison, WI, USA). The target gene was amplified by PCR using pMXs vector forward (5’GGTGGACCATCCTCTAGA3’ ...
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bioRxiv - Molecular Biology 2021Quote: ... 24 hours later cells were harvested into 125 μl reporter lysis buffer and luciferase activity and β-galactosidase levels measured using a luciferase assay kit (Promega, Madison, WI) and a luminometric β-galactosidase detection kit (Takara Bio ...
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bioRxiv - Cancer Biology 2020Quote: ... service by analyzing DNA samples from each individual cell line for polymorphic short tandem repeat (STR) markers using the GenePrint 10 kit from Promega (Madison, WI, USA).
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bioRxiv - Microbiology 2020Quote: Samples from the human subject were extracted with a DNA extraction protocol adapted from the Maxwell RSC Buccal Swab DNA kit (Catalogue number AS1640: Promega Corporation, Madison WI). Briefly ...
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bioRxiv - Microbiology 2022Quote: ... The viral RNAs were extracted from the homogenized lung tissue samples (10% w/v) and nasal wash samples using the Maxwell® RSC Viral Total Nucleic Acid Purification Kit (Promega, Madison, WI). The whole genomes of SARS-CoV-2 used in this research were amplified using a modified ARTIC protocol with several primers replaced or added (57 ...
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bioRxiv - Cancer Biology 2022Quote: ... the cells were harvested in the lysis buffer and the fluorescence value was measured by a dual luciferase assay kit (Promega, Madison, WI, USA).
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bioRxiv - Cell Biology 2022Quote: ... PCR products were purified with the Nucleospin gel and PCR clean-up kit (Macherey Nagel) and transcribed in vitro using T7 RNA polymerase (RiboMAX system; Promega, Madison, WI, USA). RNA was purified from the sample using the Nucleospin RNA clean-up kit (Macherey Nagel) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Total RNA was extracted from the antennae (50 individuals) of soldiers and workers using an SV Total RNA extraction kit (Promega, Madison, WI, USA). The amount of RNA and DNA in each sample was quantified using a Qubit fluorometer (Life Technology ...
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bioRxiv - Synthetic Biology 2022Quote: ... The viability of Vero-E6-Luc2+ cells was assessed using either CellTiter-Glo™ Luminescent Cell Viability Assay Kit (Promega, Cat# PR-G7570) or One-Glo® assay (Promega ...
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bioRxiv - Microbiology 2022Quote: ... genomic DNA extraction was performed using a lysozyme/mutanolysin-based cell lysis followed by purification using the Wizard Genomic DNA Purification Kit (Promega, Madison, WI, USA). Library preparation on extracted DNA was conducted using a Kapa kit (Roche ...
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bioRxiv - Molecular Biology 2022Quote: These recombinant plasmids were co-transfected with the components of Dharmacon™ Trans-Lentiviral packaging kit into HEK293FT cells using FuGENE® HD (Promega Corp.) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: The DNA was extracted using the Promega PCR Cleaning Kit (Wizard® SV Gel and PCR Clean-Up System, Promega Cat.-No. A9285) as indicated in the manual ...
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bioRxiv - Systems Biology 2022Quote: ... Resistance against ribociclib was detected by the alteration of the dose–response curve measured using CellTiterGlo Chemiluminescent Kit (Promega Corporation, Cat. No.: G7573).
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bioRxiv - Cancer Biology 2024Quote: ... cell viability and cytotoxicity were measured with CellTiter-Glo Luminescent Cell Viability Assay with CellTox Green Cytotoxicity Assay (Promega kits G7570 and G8741).
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bioRxiv - Neuroscience 2024Quote: NAD and NADP levels were measured using the commercially available kits NAD/NADH-Glo Assay and NADP/NADPH-Glo Assay (Promega G9071, Promega G9081), respectively ...
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bioRxiv - Microbiology 2023Quote: ... globosa gut isolates (KCTC 37188 and KCTC 37189) and skin isolates (KCTC 27541 and KCTC 27777) was extracted using the Wizard HMW DNA Extraction Kit (Promega, Madison, WI, USA), according to the manufacturer’s recommendations ...