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Citations for Promega :
5201 - 5250 of 6667 citations for Osteopontin Human OPN ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Plasmid constructs (pCAG nHA GPC4, see Table 1) were transfected with Fugene (Promega E2691)/ opti-MEM mixture according to manufacturer protocols ...
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bioRxiv - Genomics 2021Quote: ... cDNA was synthesized from 1 μg RNA using M-MLV reverse transcriptase (9PIM170, Promega) and Oligo(dT)15 primers (C110A ...
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bioRxiv - Cancer Biology 2021Quote: ... or at 25°C with Glu-C (Promega; 1:20 protease:protein w/w ratio) overnight ...
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bioRxiv - Developmental Biology 2021Quote: ... 50 μl of 0.5M TEAB and trypsin (Promega, enzyme: protein ratio of 1:50) were added to the membrane ...
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bioRxiv - Microbiology 2021Quote: ... and digested with sequencing grade trypsin (Promega, 1:50 trypsin- to-protein [wt/wt]) (Yang et al. ...
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bioRxiv - Plant Biology 2021Quote: ... The following components were added: (1) M-MLV reverse transcriptase (Promega, Cat. No. M1701), 1 mM dNTP mix ...
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bioRxiv - Bioengineering 2022Quote: ... cells in each well were lysed in 30 μl 1× passive lysis buffer (Promega). 10 μl per well of lysate was assayed using the Dual Luciferase Assay kit (Promega).
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bioRxiv - Genetics 2022Quote: Type-1 quantitative bioluminescence resonance energy transfer (qBRET) experiments utilised the NanoBRET platform (Promega) and followed the general type-1 qBRET protocol reported by Felce et al.25 The assay was performed in HEK293 cells transiently expressing POPDC1 and POPDC2 constructs which possessed a C-terminal NanoLuc luciferase or HaloTag domains ...
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bioRxiv - Plant Biology 2022Quote: ... The digestion solution was diluted to <1 M urea upon addition of trypsin (Promega, V511A ...
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bioRxiv - Microbiology 2022Quote: ... 0.1-1 µg of RNA was reversed transcribed using M-MLV Reverse Transcriptase (Promega) with dNTPs ...
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bioRxiv - Immunology 2022Quote: ... 1-2 µg RNA was reverse transcribed using AMV Reverse Transcriptase (Promega, Madison, WI). Primers were purchased from Integrated DNA Technologies (IDT ...
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bioRxiv - Microbiology 2022Quote: ... cDNAs were synthesized from 1 μg of total RNA using AMV reverse transcriptase (Promega) and used directly for quantitative PCR (qPCR ...
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bioRxiv - Microbiology 2022Quote: ... Reactions were performed with the GoTaq® Probe 1-Step RT-qPCR System (Promega) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... RT-qPCR was performed using the GoTaq® 1-Step RT-qPCR System (Promega) with an input of 50 ng RNA ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then washed in fresh C+Y medium and 1 % NanoGlo substrate (Promega) was added and luminescence was measured 20 times every 1 min in a plate reader (VarioSkan luminometer ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were digested overnight at 37 °C with 1 μg Typsin/LysC mix (Promega) and 0.01% ProteaseMAX (Promega) ...
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bioRxiv - Physiology 2022Quote: ... samples were digested overnight with trypsin (Promega; Cat# V5113; 50:1 w/w, protein:enzyme)at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... Protein enzymatic cleavage was carried out with trypsin (1:20, w/w; Promega, V5280) at 37 °C for 16 h ...
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bioRxiv - Microbiology 2022Quote: ... and the RNA stored in 1 μL of RNasin® Ribonuclease Inhibitor (Promega Corporation). RNA was quantified with the Qubit™ RNA HS Assay Kit (Invitrogen) ...
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bioRxiv - Immunology 2022Quote: ... Caspase-1 activity in supernatants was quantified using a GloMax Navigator (Promega, Madison, WI). To assess the impact of caspase-1 on MV-induced IL-1β production ...
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bioRxiv - Plant Biology 2022Quote: ... cDNA was synthesized from 1 µg of RNA using M-MLV Reverse Transcriptase (Promega) and oligo d(T)15 following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... An anti-mouse IgG-horseradish peroxidase (HRP) conjugate (1:3,000 Promega, Fitchburg, United States) was used as secondary antibody ...
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bioRxiv - Molecular Biology 2022Quote: ... and the proteins were digested with 1/100 (w/w) trypsin (Promega, Cat # V5111) overnight at 37°C ...
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bioRxiv - Immunology 2024Quote: ... with a 1:50 ratio of purified protein to Endoglycosidase H (Endo H) (Promega). After treatment ...
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bioRxiv - Cell Biology 2024Quote: ... 45 µL of 1x NanoGlo Lysis buffer with 1:1000 NanoGlo substrate (Promega, USA) was added ...
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bioRxiv - Plant Biology 2024Quote: ... cDNA was obtained from 1 μg of total RNA pretreated with DNase I (Promega) by reverse transcription reaction using ImProm-II(TM ...
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bioRxiv - Microbiology 2023Quote: ... cDNAs were synthesized from 1 μg of total RNA using AMV reverse transcriptase (Promega) and used directly for quantitative PCR (qPCR ...
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bioRxiv - Molecular Biology 2024Quote: ... Standard curves based on 1:10 dilutions of 2800 M control DNA (Promega Corporation) (0.001 – 10 ng/µL ...
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bioRxiv - Cancer Biology 2024Quote: ... RT-qPCR was performed using the GoTaq® 1-Step RT-qPCR System (Promega) with an input of 50 ng RNA ...
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bioRxiv - Developmental Biology 2024Quote: ... Reverse transcription from 1 μg of total RNA was performed with the ImPromII (Promega) kit ...
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bioRxiv - Evolutionary Biology 2024Quote: ... for 30 minutes then with 5 units/1 L RQ1 RNase-free DNase (Promega) for 10 minutes at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... the target cells were incubated with 1:250 diluted EnduRen live cell substrate (Promega). After detachment ...
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bioRxiv - Plant Biology 2023Quote: ... Samples were digested in fresh digestion buffer containing 1 μg Typsin/LysC mix (Promega) and 0.01% ProteaseMAX (Promega ...
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bioRxiv - Cell Biology 2024Quote: ... and 1 μg of RNA was reverse-transcribed using M-MLV reverse transcriptase (Promega) with random hexamer primers (Amersham).
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bioRxiv - Systems Biology 2023Quote: ... for 4 h at 37 °C and then with trypsin (Promega, 1:100, w:w) overnight at 37 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... with LysC (Wako Chemicals) at 1:50 (w/w) ratio and Trypsin (Promega, V5113) at 1:100 (w/w ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Injectors were loaded with either NanoGlo reagent (Promega, 1:250 dilution in imaging buffer) or test compounds and addition was automated by the plate reader ...
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bioRxiv - Systems Biology 2023Quote: ... 5 x 106 cells were electroporated with 1 μg of NanoLuc pNL3.1 (Promega, N1031) vector and 0.2 μg of control vector ...
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bioRxiv - Cell Biology 2023Quote: ... 1 μg of both plasmids was mixed with 6 μl FuGENE reagent (Promega E269A) in 200 μl serum free DMEM and incubated for 30 min before adding the transfection mix to the cells ...
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bioRxiv - Biochemistry 2023Quote: ... and mixed with the nanoluciferase substrate Nano-Glo (Promega, N1120, final dilution 1:200) before measuring luminescence ...
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bioRxiv - Cell Biology 2022Quote: ... and 1-2 µg of RNA was reverse transcribed into cDNA using GoScript (Promega).
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bioRxiv - Biochemistry 2023Quote: ... and mixed with the nanoluciferase substrate Nano-Glo (Promega, N1120, final dilution 1:200) before measuring luminescence ...
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bioRxiv - Biochemistry 2023Quote: ... and mixed with the nanoluciferase substrate Nano-Glo (Promega, N1120, final dilution 1:200) before measuring luminescence ...
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bioRxiv - Developmental Biology 2023Quote: ... on embryos/larvae using mouse anti-β-gal (Promega catalog number Z3781, 1:1000) and rabbit anti-mCherry (BioVision ...
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bioRxiv - Developmental Biology 2023Quote: ... rabbit and mouse anti-β-Gal (MP Biomedics #559761 and Promega #Z378A, 1:1000), rabbit and mouse anti-pH3 (Merck Millipore #06-570 and Cell signal technology #9796 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Total RNA extract (1 µg) was incubated with random primers (Promega, Madison, WI, USA) at 70°C for 5 min and subsequently cooled on ice ...
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bioRxiv - Biochemistry 2023Quote: ... 1 μL of buffer containing 35 mM iodoacetamide and Trypsin/Lys-C (Promega, V5072) was added to each sample ...
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bioRxiv - Genetics 2023Quote: ... the injected leaf cells were smeared with 1 mM luciferin (Promega, catalogue no. E1605), and the LUC signal was detected using a cooled CCD imaging device.
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bioRxiv - Neuroscience 2023Quote: ... Secondary antibodies used for western blot include anti-Rabbit-HRP (1:20000, Promega W4011); anti-Mouse-HRP (1:20000 ...
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bioRxiv - Genetics 2023Quote: ... then diluted to 1M urea and digested with trypsin (Promega, 1:50 w/w) overnight at 37°C ...