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Citations for Promega :
5201 - 5250 of 6175 citations for Mouse Caprin 1 CAPRIN1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... caspase-1 biosensor mice were weighed and injected intraperitoneally with 150 mg/kg VivoGlo Luciferin (Promega). Mice were anesthetized with 2% isoflurane/air mixture and imaged 10 minutes following administration of the luciferase substrate using the IVIS 100 Imaging system (Xenogen) ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were diluted to 1 M urea with 50mM ammonium bicarbonate and digested with chymotrypsin (Promega) at 25°C overnight ...
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bioRxiv - Cancer Biology 2021Quote: ... Total RNA from cell lines was isolated using Maxwell® 16 Instrument and the Maxwell® 16 LEV simplyRNA Cells Kit (Promega Cat.# AS1270) using the protocol provided ...
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bioRxiv - Microbiology 2019Quote: Silica-dried samples were processed using a combination of bead beating (Retsch MM400, Haan, Germany) and the Maxwell® 16 DNA Purification Kit (Promega, Madison, WI, USA) and are described in detail in supplementary information ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Individual genotyping at every generation was performed via PCR on genomic DNA extracted from larvae pseudopods (Wizard® Genomic DNA Purification Kit, Promega, Madison, WI, USA) using gene-specific primers (forward ...
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bioRxiv - Cancer Biology 2019Quote: ... DNA was extracted from the tumor tissue and blood samples previously frozen using Maxwell® RSC Tissue DNA Kit (cat# AS1610, Promega, Madison, WI, USA) and Maxwell® RSC Blood DNA Kit (cat# AS1400 ...
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bioRxiv - Physiology 2019Quote: Total RNA in the root tips (0-10 mm) was extracted using Eastep® Super Total RNA Extraction Kit (Shanghai Promega Biological Products Ltd). Gene-specific primers were designed by the National Center for Biotechnology Information (NCBI ...
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The Ets protein Pointed P1 represses Asense expression in type II neuroblasts by activating TaillessbioRxiv - Developmental Biology 2021Quote: ... Proteins were expressed from the pcDNA™3.1/His construct by the TNT® T7 Quick Coupled Transcription/Translation kit (Catalog #L1170, Promega Co., Madison, WI) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... was reverse transcribed with a GoScript™ Reverse Transcriptase cDNA reverse transcription kit according to the manufacturer’s instructions (Promega Corporation, an affiliate of Promega Biotecnologia do Brasil, Ltda). Primers were designed and synthesized by IDT-DNA (San Diego ...
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bioRxiv - Microbiology 2022Quote: ... The resulting suspension was then centrifuged at 14,000 rpm at 4°C to remove cellular fragments and the genomic DNA finally was extracted using the Wizard® Genomic DNA Purification Kit reference A1125 (Promega, Madison, WI, USA) following the bulla guidelines ...
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bioRxiv - Microbiology 2022Quote: ... then purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, USA) and quantified using a Quantus™ fluorometer (Promega, Madison, WI, USA).
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bioRxiv - Microbiology 2019Quote: ... genomic DNA were isolated from both the parental UA159 and each of the seven RSV3s using Wizard® Genomic DNA Purification Kit (Promega, Madison, WI, USA) with some modifications ...
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bioRxiv - Microbiology 2019Quote: Genomic DNA was extracted from overnight bacteria cultures from resistant and wild type parental strains using the Wizard® Genomic DNA Purification Kit (Promega, Madison, WI, USA), quantified and measured for DNA quality by Qubit High Sensitive dsDNA assay (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... faginata MAT1-1 isolate using a CTAB-chloroform DNA extraction method (van Diepen et al. 2017) and a MAT1-2 isolate using a Wizard® kit (Promega, Madison, WI, USA) and suspended in 75 µl Tris-EDTA (TE ...
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bioRxiv - Molecular Biology 2020Quote: ... purified amplicons from each single-plex TaqMan® qPCR were cloned into a plasmid vector using the pGEM®-T Easy Vector System I kit (Cat.# A1360, Promega, Walldorf, Germany) and One Shot® TOP10 (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2021Quote: ... AccuPrep® Plasmid Mini Extraction Kit (Bioneer Corporation, Republic of Korea) and Wizard® SV Gel and PCR Clean-Up System (Promega Corporation, USA) were used for plasmid and PCR product extraction and purification ...
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bioRxiv - Pathology 2020Quote: Messenger RNA was isolated from cells or slices (three slices per sample, pooled, homogenized prior to extraction) using Maxwell® LEV Simply RNA Cells/Tissue kit (Promega, Madison, Wisconsin, US). A NanoDrop® ND-1000 Spectrophotometer (Thermo Scientific ...
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted from each sample taken from captured birds and carcasses using the Wizard Genomic DNA Purification kit (Promega Corp., Madison, Wisconsin, USA) according to the manufacturer’s instructions and eluted in 125 µl of DEPC-treated water ...
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bioRxiv - Plant Biology 2020Quote: Degenerate primers specific to each gene were designed using Primer3plus software [https://primer3plus.com/] for all 17 identified CKX genes following the guidelines for primer design provided by the PCR amplification kit manufacturer (Promega Corporation, USA; Table S2). PCR amplification of the identified CKX GFMs was performed using the reaction kits selected based on the gene size ...
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bioRxiv - Physiology 2020Quote: ... were isolated from cycling Pdgfrb-BAC-eGFP mice by FACS and RNA extracted using the Maxwell® SimplyRNA cells kit (Promega, Cat. No. AS1270). cDNA was amplified using the NuGEN Ovation V2 system and all purified samples were judged to be of high quality and sufficient quantity for next generation mRNA sequencing using the LabChip method (Perkin Elmer) ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... One microgram of total RNA from each sample was reverse transcribed into first-strand cDNA by using the M-MLV kit (Promega Corporation, Madison, WI, USA) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... Primers flanking the upstream and downstream regions of each gene of interest were amplified from PA14 genomic DNA (Promega Wizard Genomic DNA Purification Kit) and extracted with GeneJet Gel Extraction Kit (ThermoFisher) ...
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bioRxiv - Microbiology 2022Quote: ... Colonies were collected and subjected to total DNA extraction using the Maxwell® 16 Tissue DNA Purification Kit in the Maxwell 16 Research Instrument (Promega, Madison, WI, USA) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA isolation was performed by Maxwell® 16 Instrument configured with the LEV High Strength Magnetic Rod and Plunger Bar Adaptor and Maxwell® 16 LEV simplyRNA Purification Kit (PROMEGA, WI, The US) by following the supplier’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... DNA fragments such as PCR products or DNA fragment as an agarose gel slices were purified using the Wizard® SV Gel and PCR Clean-Up System kit from Promega (Madison, WI, USA). DNA was digested with the appropriate restriction endonucleases (FastDigest ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: We extracted total RNA by combining a TRIZOL-chloroform approach with a spin column kit (SV Total RNA Isolation System, Promega (Peuß et al., 2016)) ...
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bioRxiv - Microbiology 2023Quote: ... The value of relative light unit of luciferase was determined using the PicaGene Luminescence Kit (TOYO B-Net Co., Ltd., Tokyo, Japan) and GloMax Navigator System G2000 (Promega Corporation, Madison, WI, USA), according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA from the bacterial strains used as positive controls was extracted using the Wizard® Genomic DNA Purification Kit (Promega, Madison, WI, U.S.) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: Cell pellets resulting from later experiments (optimization of medium composition and fiber- and drug-testing) were pre-processed using the Maxwell® HT 96 gDNA Blood Isolation Kit (Promega AG, Dübendorf, Switzerland) combined with a FastPrep homogenizer (FastPrep-24TM ...
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bioRxiv - Cancer Biology 2023Quote: ... DNA and RNA were extracted from cell pellets using the Maxwell® RSC Cultured Cells DNA and RNA Kit (Promega, AS1620 and AS1390, respectively) with the Maxwell® RSC 48 Instrument (Promega ...
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bioRxiv - Genomics 2023Quote: ... DNA extraction from pre-enrichments and selective enrichments was done using the Promega Maxwell® RSC cultured cells DNA kit (Promega, WI, USA, AS1620) according to the manufacturer’s specifications on the Promega Maxwell® RSC 48 Instrument ...
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bioRxiv - Evolutionary Biology 2024Quote: ... fruticulosa plants hydroponically cultivated under 0 mM or 150 mM NaCl was extracted using the Maxwell plant RNA kit (Promega Corporation, Madison, WI, USA) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were diluted to 1 M urea with 50 mM ambic and digested with Trypsin (Promega #V5111) for 10 hours at 1:125 enzyme:protein at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... ribosome positioning was determined by primer extension/reverse transcription using AMV reverse transcriptase (1 U/μl) (Promega) in the presence of 125 μM of each of dTTP ...
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bioRxiv - Microbiology 2019Quote: ... The pellet was dissolved in 1% (w/v) RapiGest (Waters) (10 µL) and trypsin (Sequencing grade, Promega) solution was added (90 µL ...
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bioRxiv - Molecular Biology 2021Quote: ... lysed in 1 × passive lysis buffer and analyzed using the Dual-Luciferase Reporter Assay System (Promega Corporation) and a SpectraMax L Microplate Reader (Molecular Devices ...
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bioRxiv - Microbiology 2020Quote: ... the blots were incubated for 1 hour with secondary antibody (goat anti-rabbit IgG-HRP, Promega W4018) that was diluted 1:10000 in blocking buffer ...
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bioRxiv - Microbiology 2019Quote: ... The reaction mixture (25 μL) used for cDNA synthesis contained 1⋅25 μM random hexamer primers (Promega), 250 μM of each deoxynucleoside triphosphate (dNTP) ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein digestion on beads was carried out using a ratio 1:50 sequencing grade modified trypsin (Promega): protein extract in 100 mM ammonium bicarbonate at 37 °C overnight ...
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bioRxiv - Plant Biology 2019Quote: ... using 1 - 2 µg of total RNA and 250 ng of a mixture of random hexanucleotides (Promega) and incubated for 50 min at 50°C ...
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bioRxiv - Immunology 2019Quote: ... In vitro pmel-1 T cell-mediated cytotoxicity assay with CytoTox-ONE Homogeneous Membrane Integrity Assay (Promega). protocols.io dx.doi.org/10.17504/protocols.io.6j4hcqw
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bioRxiv - Developmental Biology 2021Quote: ... the reaction mixtures for in vitro transcription were treated with RQ-1 RNase-free DNase I (Promega). Synthesized mRNAs were treated with phenol-chloroform to remove protein components ...
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bioRxiv - Biophysics 2021Quote: ... the cells were transfected with 1 – 2 μg EphA2-mTurquoise plasmid DNA using FuGene HD (Promega, #E2311) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... cells were transfected with 500 ng of either HDM-SARS-Spike-delta21 or HDM_Spike_RBD_B7-1 plasmids and 1.5 ng of Transfection Carrier DNA (Promega, E4881) using BioT reagent (Bioland Sci ...
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bioRxiv - Microbiology 2020Quote: ... RNA (1 μg) was converted to cDNA using the GoScript™ Reverse Transcription System (Promega, Madison, WI). The cDNA was synthesised was used for qPCR using the iQTM SYBR® Green Supermix (Bio-Rad Laboratories ...
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bioRxiv - Plant Biology 2021Quote: ... Dilutions of antibodies applied were: rabbit anti-ferritin 1:10000 (primary) and anti-rabbit HRP conjugated (Promega) 1:10000 (secondary).
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bioRxiv - Microbiology 2021Quote: ... At 1 × s after injection of 20 µl of the substrate solution (Renilla Luciferase Assay System, Promega), relative light units (RLUs ...
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bioRxiv - Cell Biology 2020Quote: 1 µg of total RNAs were reverse transcribed with Moloney murine leukemia virus Reverse Transcriptase (M170B, Promega). 100 ng cDNA and FastStart Universal SYBR Green Master (04913850001 ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were digested in-gel using trypsin (Gold Promega, 1 μg per band, overnight at 30°C,) as previously described (Thouvenot et al. ...
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bioRxiv - Microbiology 2022Quote: ... Larvae were immobilized in the dish using a drop of 1% low-melting-point agarose (V2111, Promega) solution in 80% glycerol ...