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Citations for Promega :
5401 - 5450 of 6175 citations for Mouse Caprin 1 CAPRIN1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: Real-time PCR was carried out using the Promega GoTaq® Probe 1-Step RT-qPCR System (Promega) with Sarbecovirus-specific envelope gene primers from bat RNA samples as published by (Corman ...
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bioRxiv - Biochemistry 2024Quote: ... were digested overnight at 37 °C using trypsin with a 1:20 trypsin to sample ratio (Promega, #V5117). The next day ...
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bioRxiv - Microbiology 2023Quote: ... the supernatants were aspirated from the wells and 50μl of a 1:8000 concentration of ViviRen (Promega, #E6491) in viral media were added to each well ...
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bioRxiv - Biophysics 2023Quote: The activity of the masked antagonists was analyzed using a PD-1/PD-L1 Blockade Bioassay (Promega, J1250) according to a modified manufacturer’s protocol as described previously(32) ...
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bioRxiv - Cell Biology 2023Quote: ... the gel plugs were digested by using 1:50 (Trypsin: Protein amount ratio) Sequencing Grade Modified Trypsin (Promega) at 37 °C overnight ...
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bioRxiv - Systems Biology 2023Quote: ... Proteins were digested off the beads by adding 6 μL of 1 μg/μL sequencing grade Trypsin (Promega), diluted in 50 mM acetic acid ...
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bioRxiv - Immunology 2022Quote: ... Cell pellets were solubilized with 10 μL of 8M urea and 10 μL of 1% Protease MAX (Promega) in 100 mM Tris-HCl ...
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bioRxiv - Microbiology 2023Quote: Real-time PCR was carried out using the Promega GoTaq® Probe 1-Step RT-qPCR System (Promega) with Sarbecovirus-specific envelope gene primers from bat RNA samples as published by (Corman ...
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bioRxiv - Microbiology 2023Quote: ... and 1:10000) were established using the genes previously cloned in pGEM-T Easy Vector (Promega, Madison, USA). The data were determined in terms of copies of the nisA and nisRK genes / μL of qPCR reaction ...
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bioRxiv - Molecular Biology 2023Quote: ... proteins were directly digested by incubation with sequencing-grade modified trypsin (1/50, w/w; Promega, Madison, Wisconsin) overnight at 37°C ...
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bioRxiv - Systems Biology 2023Quote: ... Samples were then digested by a 1:50 (trypsin to protein) ratio of sequencing grade modified trypsin (Promega) for 16 hours at 600 rpm and 25°C ...
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bioRxiv - Microbiology 2023Quote: ... beads were suspended in digestion buffer (Tris 50 mM pH 7.5, urea 2 M, 1 mM DTT and 5 µg.µl of trypsin (Promega)) for 3 min at 30°C ...
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bioRxiv - Microbiology 2023Quote: ... The pellet was resuspended in 0.1M TEAB and overnight (∼16 h) digestion with trypsin/Lys-C mix (1:25 protease:protein; Promega) at 37° C was performed with constant mixing using a thermomixer ...
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bioRxiv - Microbiology 2023Quote: ... Trypsin digest was allowed overnight at 37°C by the addition of 1 μg of trypsin (#V5111, Promega) to each sample ...
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bioRxiv - Genomics 2023Quote: ... 1 μL of each primer (10 μM) and 12.5 μL of GoTaq Green Master mix (Promega®, USA). The PCR was run in a thermocycler (BioRad ...
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bioRxiv - Microbiology 2023Quote: ... dissolved in 2 volumes of 0.1 M Tris-HCl (pH 8.0) with the addition of 1 μg of trypsin (Sequencing Grade Modified, Promega) and incubated for 12 hours at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... After that pancreatic enzyme was added at a ratio of 1:50 (Trypsin Gold, Promega, Fitchburg, WI, USA), and incubated overnight at 37°C ...
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bioRxiv - Plant Biology 2023Quote: ... Total proteome peptide pools were generated by sample digestion overnight with 1:100 sequencing grade trypsin (V5113; Promega). Generated peptide pools were quantified by Nanodrop ...
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bioRxiv - Plant Biology 2023Quote: ... 1 μg protein was used for dual luciferase transactivation assay using Dual-Luciferase® Reporter Assay System (Promega) following manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... 20,000 to 50,000 cells were lysed in the transposase reaction mix (12.5 μl 2xTD buffer, 2 μl TDE1 [Illumina], 10.25 μl nuclease-free water, and 0.25 μl 1% digitonin [Promega]) for 30 min at 37 °C ...
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bioRxiv - Systems Biology 2023Quote: ... Samples were diluted 1:4 with 50 mM Tris/HCl (pH 8.0) and sequencing grade modified trypsin (Promega) was added in an enzyme-to-substrate ratio of 1:50 ...
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bioRxiv - Systems Biology 2023Quote: ... and digested according to the manafacturer’s protocol for 1 hour at 50 °C by Trypsin Platinum (Promega, WV), digested material was dried in speedvac (Eppendorf ...
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bioRxiv - Microbiology 2023Quote: ... Standard curves were generated using purified genomic stocks (HSV-1 bacterial artificial chromosome and human genome Promega #G1471). Absolute copy number of genomic stocks was determined using droplet digital PCR (Biorad QX600) ...
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bioRxiv - Biochemistry 2023Quote: ... Tryptic digestion was performed by incubating the beads with 1 µg MS-grade TPCK trypsin (Promega, Madison, WI) dissolved in 200 µL of 50 mM ammbic (pH 8.3 ...
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bioRxiv - Microbiology 2023Quote: ... AePer50 reverse transcription (RT) and qPCR reactions were performed using GoTaq® 1-Step RT-qPCR System (Promega), with 10 µl of 2X qPCR Master Mix ...
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bioRxiv - Microbiology 2023Quote: ... and then incubated with anti-rabbit horseradish peroxidase (HRP) conjugate antibodies (1:20000 dilution) (Promega, Madison, WI, USA) for two hours at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... A 90 μl volume of each culture and 10 μl of the furimazine (1 in 200 dilution; Promega) were incubated in a Cellstar non-transparent white 96-well microplate for one h and the bioluminescence intensity was monitored with a Tecan Infinite 200 PRO plate reader every 30 s ...
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bioRxiv - Bioengineering 2024Quote: The primary antibodies used in this study were the following: rb-α-Nanoluc (non-commercial, Promega, 1:1000); rb-α-CD63 (ab68418 ...
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bioRxiv - Molecular Biology 2024Quote: ... samples were incubated with 80 μL of 2 M urea in 50 mM Tris-HCl (pH 7.5) containing 1 mM DTT and 0.5 μg trypsin (Promega) at 25°C with shaking for pre-digestion ...
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bioRxiv - Developmental Biology 2024Quote: ... From there on all buffers were supplemented with 1:100 RNasin Plus RNase inhibitor (Promega, Cat. No. N2615).
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bioRxiv - Cancer Biology 2024Quote: ... G17-1 cell proliferation was measured by MTS assay (Cell Titer 96 Aqueous One, Promega, Madison, WI, USA).
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bioRxiv - Synthetic Biology 2024Quote: ... pre-ran reaction solutions were incubated with 1 µL RNase A (4 mg/mL RNAse A solution, Promega), and 1 µL RNase One (10 units/µL RNase ONE Ribonuclease solution ...
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bioRxiv - Genomics 2019Quote: mRNA was isolated from frozen samples using the Maxwell® 16 AS2000 instrument with a Maxwell® 16 LEV simplyRNA Tissue Kit (both Promega Corporation, Madison, WI, USA). The mRNA was isolated according to protocol with a modified lysis step as described in Snoek et al ...
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bioRxiv - Bioengineering 2022Quote: ... cells were lysed with 60 μL of either Total Glutathione Lysis Reagent or Oxidized Glutathione Lysis Reagent (provided in Promega GSH/GSSG-Glo Assay kit, V6611). After lysis ...
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bioRxiv - Zoology 2023Quote: ... Skeletal architecture was examined by hand cutting tissue sections and digesting them with a mixture of 97% Nuclei Lysis Solution (Promega; from the Wizard DNA isolation kit) and 3% 20 mg/ml Proteinase K (Promega) ...
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bioRxiv - Zoology 2022Quote: ... Skeletal architecture was investigated by hand cutting tissue sections and digesting them with a mixture of 97% Nuclei Lysis Solution (Promega; from the Wizard DNA isolation kit) and 3% 20mg/ml Proteinase K (Promega) ...
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bioRxiv - Pathology 2022Quote: ... The infectious BSMV RNAs were transcribed in vitro from linearized plasmids γ-TaPDS, γ-TaCBS, γ-TaGSTU6, γ, α, and β (Petty et al., 1989) using the RiboMAX Large Scale RNA Production System-T7 kit (Promega, USA) and the Ribo m7G Cap Analog (Promega) ...
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bioRxiv - Plant Biology 2020Quote: ... First-strand cDNA was synthesized from 1 μg total RNA using the M-MLV Reverse Transcriptase (Promega, Cat. #M1705) (primers are listed in Supplementary Table 8) ...
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bioRxiv - Cancer Biology 2021Quote: ... The digested vector (200 ng) was ligated with 1 µL of the oligonucleotides (0.45nmol/μl) using T4 DNA ligase (Promega) for 3 hrs ...
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bioRxiv - Microbiology 2020Quote: ... ratio 50:1 (protein:rLysC) for 3h/37°C and then digested with Sequencing Grade Modified Trypsin (#V5111, Promega, France) ratio 50:1 (protein:trypsin ...
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bioRxiv - Microbiology 2020Quote: ... Cytosine tails (C-tails) were added to 1 µg sheared DNA using terminal deoxynucleotidyl transferase (TdT, Promega, Madison, WI). Transposon containing fragments were amplified in a PCR containing DNA from the TdT reaction as template and primers specific to the ColE1 site on the 5’ end of the transposon ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were washed twice (3000 x g for 5 min at 4°C) and resuspended in RNAse-free PBS with 1% BSA and 0.5 U/ul RNase inhibitor (Cat. N2615, Promega). Cells were then used for the 10x Genomics Chromium Single Cell System (10x Genomics ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.05% (v/v) NP-40 and 0.5% (v/v) Triton X-100) containing 20 U ml−1 of RNasin (Promega), 1 mM DTT ...
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bioRxiv - Neuroscience 2021Quote: ... cells were washed twice (3000 x g for 5 min at 4°C) and resuspended in RNAse-free PBS with 1% BSA and 0.5 U/ul RNase inhibitor (Cat. N2615, Promega). Cells were then used for the 10x Genomics Chromium Single Cell System (10x Genomics ...
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bioRxiv - Molecular Biology 2021Quote: ... Fujifilm) and then incubated for 8 h at 37 °C with sequence-grade trypsin (1□g; Promega, Madison, Wisconsin). Peptides were desalted on Ultra Micro Spin Columns C18 (The Nest Group Inc. ...
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bioRxiv - Molecular Biology 2020Quote: Gently re-suspend cells in 100 μl of 3% Glyoxal fixation solution with 1:25 RNasin Plus (Promega N261B) and incubate for 15 minutes on ice.
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bioRxiv - Molecular Biology 2021Quote: ... Total RNA was quantified to 3 μg to react 1 μg/μL random hexamer (C1181; Promega, Madison, WI, USA) at 70°C for 5 min ...
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bioRxiv - Bioengineering 2019Quote: ... Samples were then digested with mass-spectrometry grade trypsin (1:20, Trypsin:Substrate, Promega Gold Trypsin, San Luis Obispo, CA) overnight at 37°C [66] ...
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bioRxiv - Molecular Biology 2019Quote: ... Complementary DNA (cDNA) was synthesized from 1 μg of total RNA using random primers and Reverse Transcriptase System (Promega). The cDNA was used as a template for the real-time PCR amplification using PowerUp SYBR® Green Master Mix and a StepOne Plus system (Applied Biosystem ...
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bioRxiv - Immunology 2019Quote: ... 0.4 μg envelope vector (pMD2.G) and 1.6 μg hairpin-pLKO.1 vector (SHC016 control or gene specific shRNA. Fugene-6 (Promega) was used as transfection reagent ...