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Citations for Promega :
5351 - 5400 of 6175 citations for Mouse Caprin 1 CAPRIN1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... The urea concentration was then diluted with 17 μl of 50 mM HEPES pH 8.5 and after adding 1 μl of 0.2 mg/ml trypsin (Promega) in 0.2 mM HCl the digestion continued overnight at 37°C with interval mixing at 2000 RPM for 30 s every 15 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... the beads with bound recombinant proteins were blocked for 1 h at 4°C using reticulocyte lysate (Promega). Next ...
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bioRxiv - Cancer Biology 2022Quote: ... pMD2.G and the lentiviral gRNA plasmid at a 3:1:5 mass ratio using FuGENE HD (Promega) in Opti-MEM (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... sample-filters were incubated in a wet-cell adding 1:50 w/w trypsin: protein ratio (Promega, V5111) solubilized in 120 µl 50 mM TEAB ...
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bioRxiv - Plant Biology 2021Quote: ... filled with 175 μL 0.5x MS-agar medium 1% sucrose and 35μL (50 μg/mL) of Beetle Luciferin (Promega) dissolved in DMSO ...
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bioRxiv - Molecular Biology 2020Quote: ... 293T cells were transfected in 12 well plates with 1 ug of pcDNA3.4 POLGARF or pcDNA3.4 Timer (as a negative control) with Fugene transfection reagent (Promega) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... were transfected with 4.5 µg of the pBabe-puro-NCAM140 mApple construct (NCBI reference sequence: NM_000615.6, transcript variant 1) using Fugene 6 Transfection Reagent (Promega). Supernatant containing viral particles was collected and concentrated using PEG-IT (System Biosciences ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were digested by incubation with either sequencing-grade modified trypsin (1/50, w/w; Promega, Madison, Wisconsin), chymotrypsin sequencing grade (1/50 ...
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bioRxiv - Biochemistry 2021Quote: ... The sample was four-fold diluted and the protein digested in presence of 0.2 M Tris-HCl buffer pH 8.0 and 1 μg of sequencing grade trypsin (Promega) previously dissolved in 20 mM acetic acid ...
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bioRxiv - Biochemistry 2020Quote: ... and added to 50-100 µL ribosome-coupled anti-Flag M2 agarose beads and 1 µL RNasin (Promega) per reaction ...
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bioRxiv - Neuroscience 2022Quote: ... then digested at a 1:25 enzyme:protein ratio with proteomics grade trypsin/rLysC (V5071; Promega, Madison, WI, USA) overnight at 37°C according to the SP3 method (Hughes et al ...
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bioRxiv - Molecular Biology 2022Quote: ... the proteins were digested overnight at 37 °C with 6 ng μL-1 of Asp-N (Promega, UK). Peptides were extracted in 2 % v/v formic acid ...
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bioRxiv - Cancer Biology 2022Quote: ... The first step of PCR was carried out with 1 µl of cDNA and DNA polymerase GoTaq (Promega) according to the manufacturer’s instructions and consisted of 95 °C for 5 min and 15 cycles of 95 °C for 30 seconds ...
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bioRxiv - Biophysics 2019Quote: ... The sample solutions were diluted to 1 M urea with 50 mM ammonium hydrogen carbonate and trypsin (Promega) was added at an enzyme-to-substrate ratio of 1:50 ...
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bioRxiv - Molecular Biology 2019Quote: ... was transfected at a ratio of 9:1 for the candidate enhancer:constitutive promoter driving Renilla luciferase (pGL4.75, Promega). Luciferase activity was determined using the Promega Dual-Luciferase® Reporter Assay System according to manufacturer specifications on a Synergy HI plate reader (Bio-Tek).
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bioRxiv - Microbiology 2020Quote: ... A DENV-1 3’UTR specific probe was generated by PCR reaction with GoTaq Polymerase (Promega, Wisconsin, USA) containing DIG DNA-labelling mix (Roche ...
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bioRxiv - Molecular Biology 2020Quote: ... 8 μl 5x PNK pH 6.5 buffer [350 mM Tris-HCl pH 6.5, 50 mM MgCl2, 5 mM DTT], 1 μl PNK enzyme [NEB], 0.5 μl RNasin [Promega] ...
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bioRxiv - Plant Biology 2021Quote: ... The powder was resuspended in 30 mL of Extraction Buffer 1 (10mM Tris-HCl pH8; 0.4M sucrose; 10 mM MgCl2; 5 mM BME; 0.2 mM PMSF; RNasin PROMEGA) and was filter thought a Nylon membrane of 150 μm and centrifuged at 2000 g for 20 min at 4 °C ...
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bioRxiv - Immunology 2020Quote: ... Cells were then incubated with CellTiter 96 Aqueous One Solution Proliferation Solution for 1 hour (Promega, Fitchburg, WI). Absorbance of the solution was then read at 490 nm ...
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bioRxiv - Biochemistry 2020Quote: ... The eluent was equally divided into three for digestion with either: 10:1 (w/w) Trypsin Gold (Promega), 7.5:1 (w/w ...
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bioRxiv - Cancer Biology 2019Quote: ... ATP solution (5 μL of a 100 μM solution containing 0.1 μg 4:1 glycine:tyrosine peptide substrate (Promega)) was added and incubated at 37 °C for 20 min before the addition of ADP-Glo reagent (5μL ...
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bioRxiv - Immunology 2020Quote: ... the membrane was incubated with HRP-conjugated goat anti-rabbit IgG diluted 1:2000 (Promega, Madison, Wisconsin, USA) for 2 h at RT ...
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bioRxiv - Immunology 2021Quote: ... qRT-PCR was performed in 12 μL reactions using GoTaq Probe 1-step RT-qPCR System (Promega, US) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... and sequentially digested with Lys-C (Wako) (protein:enzyme ratio 1:50, o/n at RT) and trypsin (Promega) (protein:enzyme ratio 1:50 ...
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bioRxiv - Cell Biology 2019Quote: ... Samples were further diluted to 1 M urea using 50 mM HEPES (pH8) and trypsin (Promega, Madison, WI) was added at a ratio of 1:20 enzyme ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 μg of DNA-free RNA was then used for reverse transcription using M-MLV Reverse Transcriptase (Promega) in the presence of RiboLock ...
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bioRxiv - Cell Biology 2021Quote: ... was then used to transcribe 1 µg of total RNA to cDNA using Oligo(dT)15 primers (Promega). KAPA Probe Fast qPCR Master Mix (2X ...
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bioRxiv - Molecular Biology 2021Quote: 1 uL of pUG cDNA was then input into a 20 uL GoTaq PCR reaction (Promega; Cat # M7123) with the first adapter specific primer (Shukla et al. ...
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bioRxiv - Immunology 2020Quote: ... the indicated NLRP1 construct (0.02 μg) and RFP (to 1 µg) with FuGENE HD according to manufacturer’s instructions (Promega). At 24 h cells were treated with DMSO or VbP (10 µM) ...
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bioRxiv - Cancer Biology 2020Quote: pET20b-6xHis-ARF5D17 or pGEX-5x-1-hGGA3 [VHS-GAT] were transformed into BL21 (DE3) pLysS bacteria (Promega). Single colonies were grown in lysogeny broth (LB ...
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bioRxiv - Cell Biology 2020Quote: ... Isolated RNA (1 μg) was used to synthesize cDNAs using Murine Moloney Leukemia Virus reverse transcriptase (MMLV, Promega). Subsequently ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were transfected with the Piggybac plasmid plus transposase at a 3:1 ratio using Fugene HD (Promega) and selected with G418 (300 µg/mL ...
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bioRxiv - Developmental Biology 2022Quote: ... were transfected into NCCIT cells (ATCC) at ∼70% confluency using a 6:1 ratio of Fugene HD (Promega) for 48 hours in ATCC-formulated RPMI media ...
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bioRxiv - Biochemistry 2022Quote: ... Elution was conducted in 3 beads volume of proteasome buffer containing 1 μL of TEV protease (Promega, PRV6101) for 1 hr at 37°C.
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bioRxiv - Neuroscience 2023Quote: ... Digestion reaction for proteins was initially set up with 1 μg of MS grade LysC (Promega, Cat# PI90307) for 3 hours at RT and then continued overnight with addition of 2 μg of trypsin (Promega ...
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bioRxiv - Molecular Biology 2024Quote: ... The samples were diluted with 17 µL of 50 mM HEPES (pH 8.5) and digested with 1 µL of 0.2 mg/mL trypsin (Promega) in 0.2 mM HCl at 37°C with interval mixing at 2000 RPM for 30 sec every 15 min.
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bioRxiv - Biochemistry 2023Quote: ... then reverse transcribed for 1 hr at 37 °C using M-MLV reverse transcriptase (Promega, Madison, WI; M1705) in the presence of RNase inhibitor (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... HaloTag-receptor expressing mESCs were fluorescently labeled with either 1 µM of HaloTag AlexaFluor 488 ligand (Promega, G1001) or 3.5µM of HaloTag AlexaFluor 660 ligand (Promega ...
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bioRxiv - Plant Biology 2024Quote: ... and incubated for 1 h at room temperature before measuring luminescence using a GloMax Explorer plate reader (Promega). A standard curve was used for quantification of the UDP produced ...
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bioRxiv - Neuroscience 2024Quote: ... Specific amplicons were prepared using several primer pairs (Table 1) in combination with GoTaq© DNA Polymerase (Promega).
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bioRxiv - Cell Biology 2024Quote: ... Tryptic digestion was performed by incubating the beads with 1 µg MS-grade TPCK trypsin (Promega, Madison, WI) dissolved in 200 μL of 50 mM ammonium bicarbonate (pH 8.3 ...
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bioRxiv - Genetics 2024Quote: ... 1 μL cDNA was used in 10-μL reactions containing Brilliant III SYBR Green qPCR Master Mix (Promega). Biological replicates were analyzed as duplicates ...
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bioRxiv - Immunology 2024Quote: ... 100mM D-luciferin was added to Atmospheric Media at 1:1000 for 100μM D-luciferin (Promega, CAT#E1602). Cells cultured in Atmospheric Media were kept at 37⁰C in atmospheric conditions ...
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bioRxiv - Cancer Biology 2024Quote: ... then washed with 1 ml of PBS and lysed using 60 μl of Cell Culture Lysis Reagent (Promega). 20 μl of cell lysate was added to 20 μl of Luciferase Substrate (Promega) ...
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bioRxiv - Microbiology 2024Quote: ... 50 to 100 ng of template were used per reaction with 1.5 µl rRNasin 40 U.ml-1 (Promega), 5 µl 5x optimized transcription buffer (Promega) ...
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bioRxiv - Systems Biology 2023Quote: ... Proteins were digested off the beads by adding 6 μL of 1 μg/μL sequencing grade Trypsin (Promega), diluted in 50 mM acetic acid ...
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bioRxiv - Immunology 2022Quote: ... Cell pellets were solubilized with 10 μL of 8M urea and 10 μL of 1% Protease MAX (Promega) in 100 mM Tris-HCl ...
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bioRxiv - Microbiology 2023Quote: Real-time PCR was carried out using the Promega GoTaq® Probe 1-Step RT-qPCR System (Promega) with Sarbecovirus-specific envelope gene primers from bat RNA samples as published by (Corman ...
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bioRxiv - Microbiology 2023Quote: ... and 1:10000) were established using the genes previously cloned in pGEM-T Easy Vector (Promega, Madison, USA). The data were determined in terms of copies of the nisA and nisRK genes / μL of qPCR reaction ...
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bioRxiv - Microbiology 2023Quote: ... the supernatants were aspirated from the wells and 50μl of a 1:8000 concentration of ViviRen (Promega, #E6491) in viral media were added to each well ...