Labshake search
Citations for PNA Bio :
101 - 150 of 201 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2021Quote: ... we injected a mixture of guide RNA (200pg/nl total final concentration) and Cas9 protein (PNA Bio; 400 pg/nl final concentration) into one-cell stage AB embryos ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... Cas9 (PNA Bio, CP01) (500 ng/μl ...
-
bioRxiv - Developmental Biology 2021Quote: dio2vp42rc1 and tbx6vp43rc1 mutant lines were produced by injecting in-vitro transcribed single guide RNAs and recombinant Cas9 protein (PNA Bio) as previously described (Saunders et al. ...
-
bioRxiv - Developmental Biology 2021Quote: A mixture of the pooled guide RNA cocktail (400pg/μl total final concentration) and Cas9 protein (PNA Bio; 200 pg/μl final concentration) was injected into one-cell stage AB embryos ...
-
bioRxiv - Neuroscience 2021Quote: ... purified recombinant Cas9 protein (PNA Bio, 300ng/ul) and donor plasmid (500ng/ul ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... and injected (300 ng/uL) along with Cas9 protein (PNA Bio Lab: CP01, 500 ng/uL) into embryos from the BDSC 25710 line (Bassett and Liu ...
-
bioRxiv - Neuroscience 2021Quote: ... the selected gRNA pair was mixed with SpCas9 protein (PNA Bio, Thousand Oaks, CA) along with a synthetic single-strand donor DNA oligo template as described above ...
-
bioRxiv - Cell Biology 2021Quote: ... was injected in one-cell stage embryos in a solution containing Nls-CAS9 protein (PNA BIO). The mutagenesis efficacy was evaluated on pools of 30 injected embryos ...
-
bioRxiv - Genetics 2021Quote: Cas9 protein (PNA Bio, Inc.) was mixed to a final concentration of 700 ng/ul with sgRNA (37.5 ng/ul ...
-
bioRxiv - Plant Biology 2021Quote: ... and 1 μM blocking primers (mPNA and pPNA, PNA BIO, Inc., Newbury Park, CA). PCR was performed using the following specifications ...
-
bioRxiv - Cell Biology 2021Quote: ... containing 10 g of each sgRNA (#1 and #2 modified sgRNAs, Synthego) and 15 g Cas9 protein (PNA Bio) and were electroporated using program A23 on the Nucleofector™ 2b Device (Lonza) ...
-
bioRxiv - Developmental Biology 2021Quote: ... 2nL of a mixture containing 50pg/nl sgRNA with 0.5ng/nl Cas9 protein (PNA Bio CP01-20) was injected on either side of the sperm entry point at the 1-cell stage (total of 200pg sgRNA and 2ng Cas9 protein per embryo).
-
bioRxiv - Neuroscience 2021Quote: ... fertilized eggs were injected with precomplexed RNP (ribonucleoprotein)-Cas9 protein (PNA Bio, 50 ng/μl) and sgRNA (30 ng/μl) ...
-
bioRxiv - Neuroscience 2021Quote: ... sgRNA activity was confirmed by in vitro cleavage assays with purified recombinant Cas9 protein (PNA Bio, Inc., CP01-200) following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... The selected clamp (AbiesV9_01, with the sequence GTTCGCCGTCTTCGACG) was synthesized by PNA Bio, Inc ...
-
bioRxiv - Genetics 2021Quote: ... Cas9 enzyme that was injected into B6SJLF2 embryos (described below) was purchased from (PNA Bio) while the guide RNA (sgRNA ...
-
bioRxiv - Developmental Biology 2021Quote: ... one dorsal blastomere was injected with 1ng Cas9 protein (PNA Bio), 250pg shroom3-targeted sgRNA (Synthego)(Methods Appendix Fig ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... Injection solutions consisted of combinations of Cas9 recombinant protein (PNA Bio, CP-01) 1 μg μl−1 ...
-
bioRxiv - Cell Biology 2021Quote: ... Purified sgRNA (0.5 μg) was incubated with Cas9 protein (1 μg, PNA Bio) for 10 min at room temperature ...
-
bioRxiv - Developmental Biology 2021Quote: ... The concentration of Cas9 (PNA Bio) and sgRNAs varied between 125 ng/µl−250 ng/µl and 83 ng/µl−125 ng/μl ...
-
bioRxiv - Cancer Biology 2021Quote: ... containing an Alexa Fluor 488-labeled TelG PNA probe (PNA BIO F1008) at a 1:500 concentration for 2 hours at RT ...
-
bioRxiv - Genetics 2021Quote: ... ubi:delta-EGFP was generated by injecting in vitro-transcribed sg RNA and recombinant Cas9 protein (PNA Bio) into ubi:Switch zygotes (Burger et al. ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... The sgRNA mix and Cas9 endonuclease (PNA Bio, PC15111, Thousand Oaks, CA, USA) were co-injected into fertilized eggs at concentrations of 500 ng/µl and 1000 ng/µl ...
-
bioRxiv - Cancer Biology 2021Quote: ... and Alexa-Fluor 488 conjugated telG FISH probe was used (PNA BIO). For the FISH hybridization step ...
-
bioRxiv - Developmental Biology 2021Quote: ... sgRNA was mixed with Cas9 Protein (PNA Bio) and Texas Red Dextran ...
-
bioRxiv - Molecular Biology 2021Quote: ... but upstream (in frame) of the Fok1 nuclease and bGH poly(A) signals (PNA Bio Inc). In addition ...
-
bioRxiv - Developmental Biology 2021Quote: ... All plasmids were obtained from PNA Bio Inc ...
-
bioRxiv - Biophysics 2021Quote: PNAs were purchased from PNA Bio, Inc ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... A positive control Cas9 sample was made by diluting purified Cas9 protein (PNA Bio CP01) in homogenization buffer ...
-
bioRxiv - Molecular Biology 2020Quote: ... and 0.5 µg/ml CY-3 (CCCTAA)3 (PNA BIO), denatured at 75°C for 5 minutes and incubated at room temperature for 16hr ...
-
bioRxiv - Cancer Biology 2020Quote: ... Hybridization with 333ng/mL of a TelC-Cy5-labeled peptide nucleic acid (PNA) oligonucleotide telomere probe (N-CCTAACCTAACCTAA-C, PNA BIO) in PNA buffer (10mM Tris pH 7.5 ...
-
bioRxiv - Developmental Biology 2020Quote: ... Recombinant Cas9 protein was obtained commercially (PNA Bio Inc., Newbury Park, California, USA). Ribonucleoprotein complexes (RNPs ...
-
bioRxiv - Developmental Biology 2020Quote: ... Recombinant Cas9 protein with NLS sequence (800 ng/μl; PNA Bio, #CP01-20) was co-injected with each gRNA (500ng/μl ...
-
bioRxiv - Developmental Biology 2020Quote: ... Cas9 (PNA Bio, CP01) (500 ng/μl ...
-
bioRxiv - Developmental Biology 2020Quote: ... Cas9 (PNA Bio, CP01) (500 ng/μl ...
-
bioRxiv - Molecular Biology 2020Quote: ... 2018). Telomeric FISH was performed as previously described (Sfeir et al. 2009) using an Alexa488-labeled TelC PNA probe (PNA Bio Inc.). The cell cycle profiles of untreated vs ...
-
bioRxiv - Molecular Biology 2020Quote: ... Incubation with a Cy3-labeled TTAGGG3 PNA probe (PNA Bio, F1006), 50 nM in 70% formamide (Thermo Scientific ...
-
bioRxiv - Molecular Biology 2020Quote: ... RNA-protein complexes (RNPs) were prepared by mixing Cas9 protein 30-60 ug (PNA Bio) and 15-30 ug of gRNA per aliquot followed by incubation of 10 minutes at room temperature ...
-
bioRxiv - Immunology 2020Quote: ... 1 μg cas9 (PNA Bio) and 500 ng of each sgRNA were incubated on ice for 20 minutes ...
-
bioRxiv - Immunology 2020Quote: ... Cas9 protein (PNA bio) and gRNA were incubated at room temperature for 15 min in a 3:1 reaction with 15μg Cas9 and 5000 ng gRNA (2500ng of sgRNA#1 and 2500ng of sgRNA#2).47 The incubation product (gRNA and Cas9 complex ...
-
bioRxiv - Genetics 2020Quote: ... lyophilized Cas9 protein (PNA Bio Inc, CP01) was reconstituted to a stock concentration of 1 μg/μl in 20 mM Hepes ...
-
bioRxiv - Developmental Biology 2020Quote: ... esrp2 and irf6 CRISPR sgRNAs were generated by in vitro transcription from a SP6 promoter as described.(Gagnon et al., 2014) Lyophilized Cas9 protein (PNA Bio) was resuspended in ddH2O to a stock concentration of 1 μg/μl and stored in single-use aliquots in −80°C to avoid freeze-thaw inactivation and kept for 6 months ...
-
bioRxiv - Molecular Biology 2020Quote: ... CO-FISH was performed as described67 using a TelC-Alexa488-conjugated PNA probe (PNA Bio F1004) followed by a TelG-Cy3-conjugated PNA probe (PNA Bio F1006) ...
-
bioRxiv - Molecular Biology 2020Quote: ... Telomeres were detected using TelG-Alexa488-conjugated PNA probe (PNA Bio F1008) with a 1:25 dilution in hybridization solution (35% formamide ...
-
bioRxiv - Molecular Biology 2020Quote: ... followed by a TelG-Cy3-conjugated PNA probe (PNA Bio F1006). Images were captured using a Zeiss Spinning Disk confocal microscope ...
-
bioRxiv - Molecular Biology 2020Quote: FISH was performed with TelC-Cy3 (PNA Bio F1002) and CENPB-Alexa488 (F3004 ...
-
bioRxiv - Neuroscience 2020Quote: ... and donor vectors (generated by PNA Bio). The human TREM2 cDNA sequence was flanked on each side by 1-kb homology arms for the mTrem2 ...
-
bioRxiv - Neuroscience 2020Quote: ... We directly mixed recombinant Cas9 protein (300 ng/µL; PNA Bio, CP01-200), sgRNA (80 ng/µL ...
-
bioRxiv - Neuroscience 2020Quote: ... before adding recombinant Cas9 protein (300 ng/µL; PNA Bio, CP01-200) for embryo injection.
-
bioRxiv - Neuroscience 2020Quote: ... each targeting a different gene (80 ng/uL each) with Cas9 protein (300 ng/µL; PNA Bio, CP01-200) and injected into ORL embryos ...