Citations for PNA Bio :
101 - 150 of 167
citations
since 2017
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... the same protocol was carried out using a centromere probe (CENPB-AF488, PNA Bio F3004).
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bioRxiv - Genomics 2020Quote: ... and dehydrated in an ethanol series before co-denaturation of slide and PNA probes (TelG-Cy3 PNA Bio F1006, CENPB-AF488 PNA Bio F3004) for 3 mins at 80°C in hybridization solution (10 mM Tris-HCl pH 7.2 ...
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bioRxiv - Genomics 2020Quote: ... and dehydrated in an ethanol series before co-denaturation of slide and PNA probes (TelG-Cy3 PNA Bio F1006, CENPB-AF488 PNA Bio F3004) for 3 mins at 80°C in hybridization solution (10 mM Tris-HCl pH 7.2 ...
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bioRxiv - Genomics 2020Quote: ... Slides were co-denatured for 2 mins at 80°C with TelG-647 (PNA Bio F1014) in hybridization solution (10mM Tris-HCl pH 7.2 ...
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bioRxiv - Bioengineering 2020Quote: Mutation rate for each guide was determined by laser-assisted cytoplasmic injection36 of in vitro fertilized embryos with 6pL of a solution containing 67ng/μL of in vitro transcribed gRNA alongside 133ng/μL of Cas9 mRNA or 167ng/μL of Cas9 protein (PNA Bio, Inc., Newbury Park, CA) incubated at room temperature for 30 minutes prior to injection ...
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bioRxiv - Evolutionary Biology 2020Quote: ... A positive control Cas9 sample was made by diluting purified Cas9 protein (PNA Bio CP01) in homogenization buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... and 0.5 µg/ml CY-3 (CCCTAA)3 (PNA BIO), denatured at 75°C for 5 minutes and incubated at room temperature for 16hr ...
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bioRxiv - Cancer Biology 2020Quote: ... Hybridization with 333ng/mL of a TelC-Cy5-labeled peptide nucleic acid (PNA) oligonucleotide telomere probe (N-CCTAACCTAACCTAA-C, PNA BIO) in PNA buffer (10mM Tris pH 7.5 ...
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bioRxiv - Developmental Biology 2020Quote: ... Recombinant Cas9 protein was obtained commercially (PNA Bio Inc., Newbury Park, California, USA). Ribonucleoprotein complexes (RNPs ...
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bioRxiv - Developmental Biology 2020Quote: ... Recombinant Cas9 protein with NLS sequence (800 ng/μl; PNA Bio, #CP01-20) was co-injected with each gRNA (500ng/μl ...
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bioRxiv - Developmental Biology 2020Quote: ... Cas9 (PNA Bio, CP01) (500 ng/μl ...
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bioRxiv - Developmental Biology 2020Quote: ... Cas9 (PNA Bio, CP01) (500 ng/μl ...
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bioRxiv - Molecular Biology 2020Quote: ... 2018). Telomeric FISH was performed as previously described (Sfeir et al. 2009) using an Alexa488-labeled TelC PNA probe (PNA Bio Inc.). The cell cycle profiles of untreated vs ...
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bioRxiv - Molecular Biology 2020Quote: ... Incubation with a Cy3-labeled TTAGGG3 PNA probe (PNA Bio, F1006), 50 nM in 70% formamide (Thermo Scientific ...
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bioRxiv - Genetics 2019Quote: ... The TelG-FAM PNA-probe (TTAGGG repeats, PNA Bio) was used as telomere specific probe ...
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bioRxiv - Bioengineering 2019Quote: ... PNAs used in MDR clinical isolate growth experiments were ordered from PNA Bio Inc ...
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bioRxiv - Genomics 2019, published in Nature Communications doi: 10.1038/s41467-019-13596-2Quote: ... They were then mixed with Cas9-NLS protein (PNA Bio, Newbury Park, CA, USA) and diluted to a final concentration of 125-250 ng/μl ...
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bioRxiv - Bioengineering 2019, published in eLife doi: 10.7554/eLife.51701Quote: ... Synthetic gRNAs (Synthego) and recombinant Streptococcus pyogenes Cas9 protein (PNA Bio Inc.) were obtained commercially and diluted to 1,000 ng/µL in nuclease-free water and stored in aliquots at –80°C ...
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bioRxiv - Synthetic Biology 2019Quote: ... PNAs were purchased from PNA Bio, and their sequences are presented in Supplementary Table S2.
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bioRxiv - Developmental Biology 2019Quote: ... One nL of injection cocktail (100 pg/nl gRNA and 200 pg/nl Cas9 protein (PNA Bio) in water ...
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bioRxiv - Microbiology 2019Quote: ... FISH was performed using a PNA probe specific to the telomeric sequence (CCCTAA)3 (TelC-Cy5, PNA BIO).
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bioRxiv - Molecular Biology 2019Quote: ... 70% formamide and 0.1 µM Cy3 labeled (CCCTAA)3 PNA probe (PNA Bio, #F1002)) and denatured at 80°C for 3 min in a hybridization oven ...
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bioRxiv - Genetics 2019, published in Nucleic Acids Research doi: 10.1093/nar/gkz748Quote: ... and trans-activating crRNA (tracrRNA) (IDT) or single guide RNAs (sgRNA) transcribed in vitro were combined with Cas9 protein (PNA Bio Inc. or IDT) to generate complexes used for genome editing ...
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bioRxiv - Plant Biology 2019, published in Phytobiomes Journal doi: 10.1094/PBIOMES-06-19-0028-RQuote: ... and the addition of a Peptide Nucleic Acid (PNA) blocker (PNA Bio, Newbury Park, United States) at a concentration of 0.5 μM/reaction to inhibit plastidial amplification ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Injection solutions consisted of combinations of: 1 μg/μl Cas9 recombinant protein (PNA Bio, CP-01) or 400 ng/μl Cas9 mRNA (Addgene plasmid #51307 (Guo et al. ...
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bioRxiv - Microbiology 2019Quote: ... 0.375 μl mixed rRNA gene-blocking peptide nucleic acids (PNAs; 1:1 mix of 100 μM plastid PNA and 100 μM mitochondrial PNA; PNA Bio), 0.5 μl Kapa dNTPs ...
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bioRxiv - Developmental Biology 2019Quote: ... 200 pg/nl Cas9 protein (PNA Bio, Newbury Park, CA), or a combination of both reagents ...
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bioRxiv - Genomics 2019Quote: ... 10 ug Cas9 protein (PNA Bio CP01-20) was mixed with 10 ug modified guideRNA (Synthego ...
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bioRxiv - Genetics 2019Quote: ... Purified Cas9 protein (400 ng/μl) (PNA Bio, Thousand Oaks, CA) and sgRNAs (100 ng/μl ...
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bioRxiv - Microbiology 2018, published in mBio doi: 10.1128/mBio.02400-18Quote: ... Probe sequences were based on the probes used for qPCR (efficiency of hybridization: rrsH: 81; cydA: 107; cyoA: 115; appC: 73) and were synthesized by PNA Bio (Newbury Park, CA).
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bioRxiv - Genetics 2018, published in Insect Molecular Biology doi: 10.1111/imb.12528Quote: ... Each construct was pre-mixed with Cas9 protein (PNA Bio Inc., cat. # CP01-20) and in vitro transcribed sgRNAs at the following concentrations ...
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bioRxiv - Developmental Biology 2018, published in eLife doi: 10.7554/eLife.36740Quote: ... Lyophilized Cas9 (PNA Bio., Inc. Cat.# CP01) was reconstituted in nuclease-free water with 20% glycerol to a final concentration of 2µg/µl ...
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bioRxiv - Cell Biology 2018Quote: ... Validated LINGO2 guide RNA obtained commercially (PNA Bio). Briefly ...
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bioRxiv - Immunology 2018, published in Nature Medicine doi: 10.1038/s41591-018-0204-6Quote: ... at 37 °C in humidified incubators and 5% CO2 for 16 h with the following antigens: 8 μg/ml Streptococcus pyogenes (Sp) CRISPR associated protein 9 (Cas9) (SpCas9) (PNA Bio Inc., CA, USA), 1 μSEB (Sigma ...
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bioRxiv - Bioengineering 2018, published in Journal of Biological Chemistry doi: 10.1074/jbc.RA118.004554Quote: ... Cas9 protein was purchased from PNA BIO, INC ...
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bioRxiv - Developmental Biology 2018, published in Frontiers in Physiology doi: 10.3389/fphys.2019.00143Quote: ... Cas9 protein (CP01; PNA Bio) and sgRNA were incubated together at room temperature for at least 5 min prior to microinjection (DeLay et al. ...
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bioRxiv - Molecular Biology 2018, published in BMC Genomics doi: 10.1186/s12864-018-5032-zQuote: ... Recombinant Cas9 protein (PNA Bio) and a synthetic sgRNA (Synthego ...
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bioRxiv - Synthetic Biology 2018Quote: Cas9 and AAV: Recombinant SpCas9 protein (PNA Bio, cat. no. CP01), or SaCas9 protein (ABM good ...
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bioRxiv - Neuroscience 2018, published in Nature Communications doi: 10.1038/s41467-019-09074-4Quote: ... mating were co-injected with Cas9 protein (PNA Bio, 50 ng/μl) and sgRNA (30 ng/μl ...
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bioRxiv - Genetics 2018, published in PLOS Genetics doi: 10.1371/journal.pgen.1007730Quote: PNA telomere probes TelC-Alexa647 and TelC-Cy3 were acquired from PNA Bio Inc ...
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bioRxiv - Biochemistry 2018, published in Nature Communications doi: 10.1038/s41467-019-11047-6Quote: ... One-cell fertilized C57BL/6J embryos derived from mice obtained from Jackson laboratories were microinjected with a mixture of both gRNAs (25 ng/ul each) and Cas9 protein (PNA Bio, 40 ng/ul), and then implanted into pseudopregnant B6 recipients ...
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bioRxiv - Developmental Biology 2018Quote: ... tracrRna (IDT) and Cas9 protein (PNA Bio) and complexed for 10 minutes at room temperature then placed on ice ...
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bioRxiv - Developmental Biology 2018, published in eLife doi: 10.7554/eLife.42930Quote: ... The hybridised gRNAs were then incubated with the Cas9 protein (PNA bio) at 30 ng/μl for 15 min at RT and microinjected into the pronuclei of (CBAxC57 ...
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bioRxiv - Genomics 2018Quote: Double knockout dCas9 protein D10A/H840A (no cleavage activity) was purchased from PNA Bio (cat #CD01) and was diluted to a stock concentration of 1 mg/mL or 6 μM with the provided diluent.
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bioRxiv - Cell Biology 2018Quote: ... Validated Lingo3 guide RNA obtained commercially (PNA Bio). Briefly ...
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bioRxiv - Cell Biology 2018Quote: ... 1μg validated Lingo3 sgRNA (PNA Bio), 6μg Lingo3 donor vector (PNA Bio ...
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bioRxiv - Cell Biology 2018Quote: ... 6μg Lingo3 donor vector (PNA Bio) and 1μg Cas9 plasmid (PNA Bio ...
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bioRxiv - Cell Biology 2018Quote: ... and 1μg Cas9 plasmid (PNA Bio) were cotransfected into the cells using X-tremeGENE HP DNA transfection reagent (Roche) ...
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bioRxiv - Genetics 2018, published in Molecular Biology of the Cell doi: 10.1091/mbc.E19-08-0447Quote: ... and then hybridized overnight with a Cy3 Tel-C PNA probe (PNA Bio) at room temperature in a hybridization chamber ...
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bioRxiv - Cell Biology 2018, published in The FASEB Journal doi: 10.1096/fj.201902376rQuote: ... 5% maleic blocking reagent and 25µg/ml Cy3-conjugated PNA Tel-C (CCCTAA)3 probe (PNA Bio Inc.) and incubated O.N ...